Abstract:
:The activity of the bimodal fluorescent protein (bmFP) (lambda max, 488 and 517 nm) in the in vitro luciferase reaction has been studied. The bmFP that is produced by Photobacterium phosphoreum strain bmFP is a dimer of two homologous subunits binding four riboflavin 5'-phosphate (FMN)-myristate chromophores. The addition of bmFP to the luciferase reaction in the presence of the lumazine protein prevented the lumazine protein-induced blue shift in the emission band. The bmFP reduced electrochemically serves as a substrate in the luciferase reaction in the absence of added FMN, resulting in light emission with a single maximum at about 487 nm. The bmFP was also active in lieu of FMN in the NADH/FMN oxidoreductase (flavin reductase)-luciferase coupled bioluminescence reaction in the absence of added FMN. In the coupled reaction, bioluminescence with the isolated bmFP chromophore was weaker than that with the holo-bmFP. After bmFP was used in luciferase reactions initiated either chemically or electrochemically, it was still capable of emitting bimodal fluorescence.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Karatani H,Konaka Tdoi
10.1562/0031-8655(2000)071<0237:aotbfp>2.0.co;2subject
Has Abstractpub_date
2000-02-01 00:00:00pages
237-42issue
2eissn
0031-8655issn
1751-1097journal_volume
71pub_type
杂志文章abstract::Lutetium texaphyrin, PCI-0123, is a pure, water-soluble photosensitizer with a large broad absorption band centered at 732 nm. The compound was tested for photodynamic therapy (PDT) effectiveness in a murine mammary cancer model. The texaphyrin macrocycle as illustrated by magnetic resonance imaging and 14C-radiolabel...
journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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doi:
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doi:10.1562/0031-8655(2004)080<0456:EONOOO>2.0.CO;2
更新日期:2004-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00782.x
更新日期:2010-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb09154.x
更新日期:1995-11-01 00:00:00
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doi:10.1111/php.12499
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1989-05-01 00:00:00
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01201.x
更新日期:2013-01-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/2006-02-28-IR-820
更新日期:2007-01-01 00:00:00
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journal_title:Photochemistry and photobiology
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doi:10.1111/php.12404
更新日期:2015-03-01 00:00:00