Genetic variability in the response of normal murine hematopoietic progenitor cells to extracorporeal photochemotherapy.

Abstract:

:Normal hematopoietic progenitor cells from 129S6/SvEv mice are substantially less sensitive to Merocyanine 540 (MC540)-mediated photodynamic therapy (PDT) than hematopoietic progenitors from sex- and age-matched C57BL/6 mice. When exposed to a combination of MC540 and light commonly used for the extracorporeal purging of hematopoietic stem cells, granulocyte/macrophage progenitors (CFU-GM) from C57BL/6 mice are depleted 7.9-fold whereas CFU-GM from 129S6/SvEv and (C57BL/6 x 129S6/SvEv) F1 mice are depleted 1.4- and 2-fold, respectively. The same rank order of sensitivity is also found with regard to unipotent progenitors of granulocytes and macrophages and with regard to early and late erythroid progenitors. The resistance of hematopoietic progenitors from 129S6/SvEv mice to MC540-PDT appears to be the result of reduced dye binding rather than the result of high levels of intracellular glutathione. These findings have practical implications for the design of preclinical tests of PDT in animal models. They may also provide a useful tool for future investigations into the molecular determinants of sensitivity to MC540-PDT.

journal_name

Photochem Photobiol

authors

Tsujino I,Sieber F

doi

10.1562/0031-8655(2000)072<0810:gvitro>2.0.co;2

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

810-4

issue

6

eissn

0031-8655

issn

1751-1097

journal_volume

72

pub_type

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