Abstract：
:Persister bacteria tolerate bactericidal antibiotics due to transient and reversible phenotypic changes. As these bacteria can limit the effectiveness of antibiotics to eradicate certain infections, their elimination is a relevant issue. Photodynamic therapy seems suitable for this purpose, but phenotypic tolerance to it has also been reported for Pseudomonas aeruginosa. To test whether any phenotypic feature could confer tolerance against both antibiotics and photoinactivation, survivors from exposures to light in the presence of methylene blue were treated with ofloxacin, an antibiotic effective on nongrowing bacteria. Susceptibility to ofloxacin was normal in these bacteria in spite of their increased ability to survive photodynamic inactivation, suggesting the absence of cross-tolerance. It thus seemed possible to use one of these treatments to eliminate bacteria which had phenotypic tolerance to the other. To test this strategy, persister bacteria emerging from ofloxacin treatments were submitted to the action of light and methylene blue while the antibiotic remained in the bacterial suspension. Persisters lost their clonogenic ability under these conditions and the effects of the treatments seemed to be synergistic. These observations suggest that photodynamic antimicrobial therapy could be used as a complement to antibiotic treatments to eliminate persister bacteria from localized infections.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Oppezzo OJ,Forte Giacobone AFdoi
10.1111/php.12843subject
Has Abstractpub_date
2018-01-01 00:00:00pages
186-189issue
1eissn
0031-8655issn
1751-1097journal_volume
94pub_type
杂志文章abstract：:Several studies in the literature indicate that histones (lysine rich proteins found associated with DNA in eukaryotic chromatin), as well as poly-L-lysine, can be photocross-linked by ultraviolet (UV) light to DNA in which 5-bromo-2'-deoxyuridine has been substituted for thymidine. To gain some insight into the possi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1991.tb08486.x
更新日期：1991-05-01 00:00:00
abstract：:The design, synthesis and application of a new fluorescent-labeling reagent for collagen has been developed as a prerequisite for the design of a photoactivated collagen-crosslinking compound for surgical wound closure. The amine groups in collagen are the targets of a rational design for a new fluorophore because nat...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0473:pcdflf>2.0.co;2
更新日期：2002-11-01 00:00:00
abstract：:Melatonin (N-acetyl-5-methoxytryptamine) is a naturally occurring hormone with a structural resemblance to tryptophan. Its fluorescence is solvent dependent and strongly quenched in the presence of oxygen. Fluorescence quenching in aqueous and organic solutions have been studied in the presence of different quenchers....
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(1999)069<0294:pomide>2.3.co;2
更新日期：1999-03-01 00:00:00
abstract：:An alkyne-terminated anthracene and azide-terminated carbazole were joined through a copper-catalyzed cycloaddition to form a joined donor/acceptor pair. The photonic pair exhibited energy transfer when excited at the peak absorbance of carbazole and fluoresced with an anthracene spectral response. The fluorescent beh...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01023.x
更新日期：2012-01-01 00:00:00
abstract：:Oculocutaneous albinism (OCA) is a genetically inherited autosomal recessive condition. Individuals with OCA lack melanin and therefore are susceptible to the harmful effects of solar ultraviolet radiation, including extreme sun sensitivity, photophobia and skin cancer. OCA is a grave public health issue in sub-Sahara...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12359
更新日期：2015-01-01 00:00:00
abstract：:There is a need to improve the selectivity of photodynamic therapy and for better targeting of tumor cells within specific tumor compartments. Selective in vitro phototoxicity of a human bladder carcinoma cell line 647V has been achieved by targeting sulfonated aluminum phthalocyanines (AlSPc) with monoclonal antibodi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05139.x
更新日期：1994-11-01 00:00:00
abstract：:Polysulphone (PS) dosimetry has been a widely used technique for more than 30 years to quantify the erythemally effective UV dose received by anatomic sites (personal exposure). The calibration of PS dosimeters is an important issue as their spectral response is different from the erythemal action spectrum. It is perf...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01006.x
更新日期：2012-01-01 00:00:00
abstract：:Consecutive spectral analyses of ultraweak photon emission from sweet potato showing a defense response were conducted to observe the process of physiological transition. The spectrum showed a drastic transition from 2 to 10 h after inoculation with Fusarium oxysporum, during which the emission intensity increased slo...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0322:ssoupe>2.0.co;2
更新日期：2002-03-01 00:00:00
abstract：:Drug delivery systems (DDS) capable of releasing an active molecule at the appropriate site and at a rate that adjusts in response to the progression of the disease or to certain functions/biorhythms of the organism are particularly appealing. Biocompatible materials sensitive to certain physiological variables or ext...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2008.00530.x
更新日期：2009-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12499
更新日期：2015-09-01 00:00:00
abstract：:Cyanines are emerging as useful agents for photoreleasing biological compounds because of their capability of utilizing near-infrared (NIR) light. Another benefit is their ability to self-sensitize to produce singlet oxygen for the release of aryl amines, a process that has not been as feasible in the past. Here, we h...
journal_title：Photochemistry and photobiology
pub_type： 评论,杂志文章
doi：10.1111/php.13102
更新日期：2019-09-01 00:00:00
abstract：:In this study, water-soaked seeds of sunflower were exposed to He-Ne laser irradiation of different energies to determine whether or not He-Ne laser irradiation caused changes to seed thermodynamic and germination parameters as well as effects on the activities of germination enzymes. The experiment comprised four ene...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00782.x
更新日期：2010-09-01 00:00:00
abstract：:The photochemistry of caged compounds of the o-nitrobenzyl type has been investigated thoroughly in the past. However, even recently new side reactions have been discovered. Earlier, we reported [Bley, F., K. Schaper, and H. Görner (2008), Photochem. Photobiol.84 162-171] that we found long-lived triplet states which ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00560.x
更新日期：2009-09-01 00:00:00
abstract：:The distribution of anthocyanins in grape (Vitis vinifera L.) bunches from the Sangiovese cultivar was measured nondestructively by chlorophyll fluorescence imaging using two excitation light bands at 550 and 650 nm in sequence. The pixel intensity in the derived logarithm of the fluorescence excitation ratio image wa...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00424.x
更新日期：2008-11-01 00:00:00
abstract：:Changes in the emission fluorescence intensity of pheophorbide-a (PHEO) in the presence of carboquone (CARBOQ) were used to obtain the association constant, the number of CARBOQ molecules interacting with PHEO, and the fluorescence quantum yield of the complex. Excitation spectra of mixtures of PHEO and CARBOQ in etha...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00869.x
更新日期：2011-03-01 00:00:00
abstract：:Efficacy of ionizing radiation (I/R) was compared with phototoxic effects of photodynamic therapy (PDT) in vitro using two cell lines derived from patients with head and neck squamous cell carcinoma (HNSCC). A cell line derived from a donor with a human papilloma virus (HPV) infection was more responsive to I/R but si...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13150
更新日期：2020-05-01 00:00:00
abstract：:Solar ultraviolet B (UVB) radiation has been shown to induce inflammation, DNA damage, p53 mutations and alterations in signaling pathways eventually leading to skin cancer. In this study, we investigated whether fisetin reduces inflammatory responses and modulates PI3K/AKT/NFκB cell survival signaling pathways in UVB...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12337
更新日期：2015-01-01 00:00:00
abstract：:Neutrophils stimulated with formyl-methionyl-leucyl-phenylalanine (fMLP) or leukotriene B4 (LTB4) generated kinetically distinctive luminol augmented chemiluminescence (LCL). Inhibitors of .O2- [superoxide-dismutase (SOD) or tiron], H2O2 (catalase), myeloperoxidase, MPO, (NaN3), HOCl (taurine) and .OH (mannitol) hampe...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04099.x
更新日期：1989-02-01 00:00:00
abstract：:Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01736.x
更新日期：1990-04-01 00:00:00
abstract：:An ongoing study in our laboratories is to examine the relationship of DNA repair defects to human cancer. Our underlying hypothesis has been that human tumors may arise that lack interesting DNA repair pathways if these pathways are important in preventing cancer. In this study, we found that the UV-irradiated adenov...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-08-08-RA-641
更新日期：2006-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-12-13-RA-752
更新日期：2006-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.1989.tb04181.x
更新日期：1989-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12975
更新日期：2019-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)078<0603:SCFBSU>2.0.CO;2
更新日期：2003-12-01 00:00:00
abstract：:A 24 h preculture of HT29-18 human colonic adenocarcinoma cells with the sterol synthesis inhibitor lovastatin at concentrations of 0.1-0.5 microM markedly increased the photocytotoxic effect of photofrin II delivered to cells by low density lipoproteins. Under the same conditions, LDL binding and photofrin II (PII) u...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02303.x
更新日期：1993-02-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1562/2006-10-23-IR-1065
更新日期：2007-03-01 00:00:00
abstract：:The Photochemical Research Group from San Luis (Argentina) highlights the contributions of Dr. Norman Andino García to the development of the group as a way to show him our gratitude for all his support. ...
journal_title：Photochemistry and photobiology
pub_type： 社论
doi：10.1111/php.12988
更新日期：2018-11-01 00:00:00
abstract：:Because metallothionein (MT) is elevated and may be protective in UV-irradiated skin, we have studied the effects of UV and other agents on MT transcription using the sheep MT 1A promoter, linked to the beta-galactosidase gene and stably transfected into human cell lines. beta-galactosidase reporter activity was induc...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb08603.x
更新日期：1997-03-01 00:00:00
abstract：:Paracoccidioidomycosis is the most prevalent human mycosis in Latin America. Cutaneous lesions are extremely painful and sensitive, and current treatment with antifungal drugs is lengthy and may cause side effects to patients. In this perspective, the helium-neon (HeNe) laser emerges as a novel therapy form due to its...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00734.x
更新日期：2010-07-01 00:00:00
