Abstract：
:In eukaryotic cells helix-distorting DNA lesions like cyclobutane pyrimidine dimers (CPDs) and 6-4 pyrimidine-pyrimidone photoproducts (6-4 PPs) are efficiently removed by nucleotide excision repair (NER). NER is a multistep process where in the end, subsequent to replication over the gap, the remaining nick is sealed by a DNA ligase. Lig1 has been implicated as the major DNA ligase in NER. Recently, Lig3 has been implicated as a component of a NER subpathway that operates in dividing cells, but which becomes particularly important in nondividing cells. Here, we use DT40 cells and powerful gene targeting approaches for generating DNA ligase mutants to examine the involvement and contribution of Lig1 and Lig3 in NER using cell survival measured by colony formation, and repair kinetics of CPD by immunofluorescence microscopy and immuno-slot-blotting. Our results demonstrate an impressive and previously undocumented potential of Lig3 to substitute for Lig1 in removing helix-distorting DNA lesions by NER in proliferating cells. We show for the first time in a clean genetic background a functional redundancy in NER between Lig1 and Lig3, which appears to be cell cycle independent and which is likely to contribute to the stability of vertebrate genomes.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Paul-Konietzko K,Thomale J,Arakawa H,Iliakis Gdoi
10.1111/php.12487subject
Has Abstractpub_date
2015-09-01 00:00:00pages
1173-80issue
5eissn
0031-8655issn
1751-1097journal_volume
91pub_type
杂志文章abstract：:Ultraviolet-A irradiation of a suprofen (2-[4-2(2-thenoyl) phenyl]propionic acid) (SPF) buffered solution (pH 7.4) in the presence of supercoiled pBR322 DNA leads to single strand breaks with the formation of an open circular form and subsequent linearization of the plasmid. On the basis of agarose gel electrophoresis...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb05252.x
更新日期：1995-07-01 00:00:00
abstract：:In recent years the class of known fluorescent proteins (FPs) has dramatically expanded as an ever-increasing numbers of variants and homologs of the green fluorescent protein (GFP) from Aequorea jellyfish have been either engineered in the lab or discovered in other marine organisms. The red fluorescent protein (RFP)...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00206.x
更新日期：2008-01-01 00:00:00
abstract：:Angelica archangelia (AA), a traditional herb, has attracted attention as an agent with potential for use in the prevention of chronic skin diseases. This study examined the photoprotective effects of AA on the inhibition of matrix metalloproteinases (MMPs) and collagen degradation in UVB-irradiated normal human derma...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12595
更新日期：2016-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12671
更新日期：2017-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2008.00459.x
更新日期：2008-11-01 00:00:00
abstract：:Elemental sulfur is a low-cost and abundant substance as one of the largest by-products of the oil industry which was widely used in many industrial activities. Cyclo-octasulfur (S8 ) is one of the sulfur allotropes that is a very stable substance in standard conditions. In this study, we report a low-cost and fast me...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13301
更新日期：2020-11-01 00:00:00
abstract：:Fabrea salina is a marine ciliate that shows photomotile responses such as positive phototaxis and a step-down photophobic reaction. We found that preilluminated F. salina cells show a phototactic response significantly greater than that of dark-adapted cells when exposed to the same phototactic light stimulus. In par...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)077<0204:eopopr>2.0.co;2
更新日期：2003-02-01 00:00:00
abstract：:The tetraruthenated porphyrin, mu-[meso-5,10,15,20-tetra(pyridyl)porphyrin]tetrakis[bis-(bipyridine) chloride ruthenium(II)] (TRP) is a supramolecular cationic species. The aim of the present investigation was to evaluate the photodynamic properties of TRP and Zn-TRP to damage DNA with emphasis on the mechanistic aspe...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:A visual chromophore analogue, 10-methyl-13-demethyl (dm) retinal, was synthesized and reconstituted with bleached bovine rhodopsin to form a visual pigment derivative with absorbance maximum at 505 nm. The investigations with this new compound were stimulated from recent results using 13-dm retinal as a chromophore t...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb01896.x
更新日期：1997-01-01 00:00:00
abstract：:Photodynamic therapy (PDT), carried out at low fluence rates, may enhance tumor response as well as affect treatment selectivity. We have studied the effects of fluence rate on the response of the murine radiation-induced fibrosarcoma (RIF) to PDT using Photofrin (5 mg/kg). Tumor response was tested over a large range...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05080.x
更新日期：1994-08-01 00:00:00
abstract：:Excessive sun exposure can cause sunburn, suntan, skin photoaging, and even skin cancer. Skin photoaging conflicts with the human pursuit of a young and beautiful appearance. Some research data indicate that the incidence of skin cancer in the Chinese has been increasing, although it remains lower than in whites. To e...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12623
更新日期：2016-09-01 00:00:00
abstract：:The proton channels of the bacteriorhodopsin (BR) proton pump contain bound water molecules. The channels connect the purple membrane surfaces with the protonated retinal Schiff base at the membrane center. Films of purple membrane equilibrated at low relative humidity display a shift of the 570 nm retinal absorbance ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0714:wacgei>2.0.co;2
更新日期：2000-11-01 00:00:00
abstract：:Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0060:cbramu>2.0.co;2
更新日期：2000-01-01 00:00:00
abstract：:Owing to the accessibility of skin to light, many applications of photodynamic treatment (PDT) have been developed within dermatology. The recent increase of dermatological antimicrobial PDT investigations is related to the growing problem of bacterial and fungal resistance to antibiotics. This review focuses on the s...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2010.00848.x
更新日期：2011-01-01 00:00:00
abstract：:Several studies in the literature indicate that histones (lysine rich proteins found associated with DNA in eukaryotic chromatin), as well as poly-L-lysine, can be photocross-linked by ultraviolet (UV) light to DNA in which 5-bromo-2'-deoxyuridine has been substituted for thymidine. To gain some insight into the possi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1991.tb08486.x
更新日期：1991-05-01 00:00:00
abstract：:Proton magnetic resonance imaging (MRI) and histological methods were used to evaluate photodynamic therapy (PDT)-induced hemorrhagic necrosis in the murine M1 tumor within 72 h of treatment of male DBA/2 mice. The effects of three photosensitizing drugs were investigated: Photofrin (n = 4), Zn (II) phthalocyanine (n ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb03236.x
更新日期：1997-12-01 00:00:00
abstract：:The efficacy of photodynamic therapy (PDT) depends upon the amount of photosensitizer accumulated in the malignant tissues. Radachlorin is a popular photosensitizer used in photodynamic therapy to treat various types of cancer. In this study, we have studied the main organelles responsible for the accumulation of rada...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12273
更新日期：2014-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0527:cwmine>2.0.co;2
更新日期：2002-05-01 00:00:00
abstract：:The psaI and psaL genes were characterized from the cyanobacterium Synechococcus sp. strain PCC 7002. The gene organization was different from that reported for other cyanobacteria with psaI occurring upstream and being divergently transcribed from the psaL gene. Mutants lacking PsaI or PsaL were generated by interpos...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb02421.x
更新日期：1996-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:The photophysical properties of 1,1'-diethyl-4,4'-carbocyanine chloride (kryptocyanine) have been measured in methanol solution and for the dye bound to human serum albumin, incorporated in neutral micelles and after incubation with leukemia cells. In all cases, it is found that formation of the triplet state of the d...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb08674.x
更新日期：1990-10-01 00:00:00
abstract：:On direct photoexcitation, subpicosecond time-resolved absorption spectroscopy revealed that the 1B(u)-type singlet excited state of all-trans-lycopene in chloroform was about seven times more efficient than all-trans-beta-carotene in generating the radical cation. The time constant of radical cation generation from t...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-04-22-RA-147
更新日期：2004-09-01 00:00:00
abstract：:The energy and oscillator strength of electronic transitions of chlorophyll (Chl)-amino acid complexes were calculated by using molecular orbital methods. The energies varied widely with coordinated amino acids and the difference between the maximum and minimum energy was about 830 cm-1. This energy difference was com...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0245:aomoct>2.0.co;2
更新日期：2001-03-01 00:00:00
abstract：:Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expres...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01232.x
更新日期：2013-03-01 00:00:00
abstract：:Photodynamic treatment of promyelocytic K562 cells in the presence of a monoglucosylporphyrin or hematoporphyrin leads to a sequence of events recognized as hallmarks of apoptosis: a drop in mitochondrial potential, concurrent with a drop in ATP level and a decrease in cell respiration, translocation of phosphatidylse...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The effect of pretreatment with vitamin E on cytotoxicity, DNA single strand breaks, and chromosomal aberrations as well as on mutation induced by ultraviolet-B light (UV-B) was investigated in Chinese hamster V-79 cells. Cellular pretreatment with non-toxic levels of 25 microM alpha-tocopherol succinate (vitamin E) f...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb09598.x
更新日期：1992-07-01 00:00:00
abstract：:In current clinical practice, photodynamic therapy (PDT) is carried out with prescribed drug doses and light doses as well as fixed drug-light intervals and illumination fluence rates. This approach can result in undesirable treatment outcomes of either overtreatment or undertreatment because of biological variations ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0398:mpfapb>2.0.co;2
更新日期：2002-04-01 00:00:00
abstract：:A structural subunit (called B816) has been isolated from the B875 light-harvesting complex of Rhodobacter capsulatus using a detergent-mediated dissociation of chromatophores. Rb. capsulatus MW442 (B800-850- B875+ car+) chromatophores were extracted with benzene and titrated with octyl glucoside (OG) to shift the nea...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01975.x
更新日期：1990-05-01 00:00:00
