Are the fluorescent properties of the cyan fluorescent protein sensitive to conditions of oxidative stress?

Abstract:

:The modifications induced by reactive oxygen species (ROS) on fluorescent proteins (FPs) may have important implications for live cell fluorescence imaging. Using quantitative gamma-radiolysis, we have studied the ROS-induced biochemical and photophysical perturbations on recombinant cyan fluorescent protein (CFP). After oxidation by the OH radical, the protein displays a modified RP-HPLC elution profile, while the CFP fluorescence undergoes pronounced decreases in intensity and lifetime, without changes in its excitation and emission spectra. Meanwhile, the Förster resonant energy transfer (FRET) between the single W(57) and the chromophore remains unperturbed. These results rule out a direct oxidation of the CFP chromophore and of W(57) as well as major changes in the protein 3D structure, but show that new fluorescent forms associated to a higher level of dynamic quenching have been generated. Thus, strict in situ controls are required when CFP is to be used for FRET studies in situations of oxidative activity, or under strong illumination.

journal_name

Photochem Photobiol

authors

Alvarez L,Levin CH,Merola F,Bizouarn T,Pasquier H,Baciou L,Rusconi F,Erard M

doi

10.1111/j.1751-1097.2009.00617.x

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

55-61

issue

1

eissn

0031-8655

issn

1751-1097

pii

PHP617

journal_volume

86

pub_type

杂志文章
  • Chronology of the apoptotic events induced in the K562 cell line by photodynamic treatment with hematoporphyrin and monoglucosylporphyrin.

    abstract::Photodynamic treatment of promyelocytic K562 cells in the presence of a monoglucosylporphyrin or hematoporphyrin leads to a sequence of events recognized as hallmarks of apoptosis: a drop in mitochondrial potential, concurrent with a drop in ATP level and a decrease in cell respiration, translocation of phosphatidylse...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Carré V,Jayat C,Granet R,Krausz P,Guilloton M

    更新日期:1999-01-01 00:00:00

  • Excited state processes in 1-deazariboflavin studied by ultrafast fluorescence kinetics.

    abstract::The photochemical reactions of the 1-deaza derivative of riboflavin (RF) have been studied by sub-ps time-resolved fluorescence spectroscopy. A potential effect of the solvent polarity and hydrogen bonding capability on the excited state (ES) dynamics was tested by measuring 1-deaza RF in water and, after acetylation ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00646.x

    authors: Slavov C,Mansurova M,Holzwarth AR,Gärtner W

    更新日期:2010-01-01 00:00:00

  • Increasing Temperature Counteracts the Negative Effect of UV Radiation on Growth and Photosynthetic Efficiency of Microcystis aeruginosa and Raphidiopsis raciborskii.

    abstract::High temperature can promote cyanobacterial blooms, whereas ultraviolet radiation (UVR) can potentially depress cyanobacterial growth by damaging their photosynthetic apparatus. Although the damaging effect of UVR has been well documented, reports on the interactive effects of UV radiation exposure and warming on cyan...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13377

    authors: Noyma NP,Mesquita MCB,Roland F,Marinho MM,Huszar VLM,Lürling M

    更新日期:2021-01-04 00:00:00

  • Wavelength dependence of histological, physical, and visible changes in chronically UV-irradiated hairless mouse skin.

    abstract::Albino hairless mice (Skh: HR-1) exposed chronically to sub-erythemal doses of UV radiation display physical, visible and histological alterations. Using narrow bandwidth radiation covering the UV radiation spectrum from 280-380 nm, the wavelength dependence of these alterations was determined. The wavelength dependen...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb02907.x

    authors: Bissett DL,Hannon DP,Orr TV

    更新日期:1989-12-01 00:00:00

  • Comparative analysis of GPCR crystal structures.

    abstract::The phototransduction cascade is perhaps the best understood model system for G protein-coupled receptor (GPCR) signaling. Phototransduction links the absorption of a single photon of light to a decrease in cytosolic cGMP. Depletion of the cGMP pool induces closure of cGMP-gated cation channels resulting in the hyperp...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1751-1097.2008.00516.x

    authors: Lodowski DT,Angel TE,Palczewski K

    更新日期:2009-03-01 00:00:00

  • Photoinduced degradation of the herbicide clomazone model reactions for natural and technical systems.

    abstract::The photodegradation of the herbicide clomazone in the presence of S(2)O(8) (2-) or of humic substances of different origin was investigated. A value of (9.4 +/- 0.4) x 10(8) m(-1) s(-1) was measured for the bimolecular rate constant for the reaction of sulfate radicals with clomazone in flash-photolysis experiments. ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00467.x

    authors: David Gara PM,Bosio GN,Arce VB,Poulsen L,Ogilby PR,Giudici R,Gonzalez MC,Mártire DO

    更新日期:2009-05-01 00:00:00

  • Rational Design, Synthesis and Evaluation of Indole Nitrogen Hybrids as Photosystem II Inhibitors.

    abstract::We report the synthesis of twelve indole derivatives bearing nitro or amide groups via Fischer indole methodology followed by reduction/acetylation and amidation reactions. After thorough characterization, these indoles were subjected to a number of studies in order to evaluate their bioactive potential as photosynthe...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13295

    authors: de Souza JM,Fazolo BR,Lacerda JWF,Moura MS,Dos Santos ACR,de Vasconcelos LG,de Sousa Junior PT,Dall'Oglio EL,Ali A,Sampaio OM,Vieira LCC

    更新日期:2020-11-01 00:00:00

  • Fluorescence lifetimes of protochlorophyllide in plants with different proportions of short-wavelength and long-wavelength protochlorophyllide spectral forms.

    abstract::Dark-grown leaves of maize (Zea mays), wheat (Triticum aestivum), wild-type pea (Pisum sativum) and its light-independent photomorphogenesis mutant (lip1) have different proportions of protochlorophyllide (Pchlide) forms as revealed by low-temperature fluorescence emission spectra. Four discrete spectral forms of Pchl...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)078<0205:flopip>2.0.co;2

    authors: Myśliwa-Kurdziel B,Amirjani MR,Strzałka K,Sundqvist C

    更新日期:2003-08-01 00:00:00

  • Ag Nanoparticles Connected to the Surface of TiO2 Electrostatically for Antibacterial Photoinactivation Studies.

    abstract::Supported silver nanoparticles (Ag NPs) were prepared by chemical reduction method with a sol-gel method. The structure, morphology, and interconnectivity of Ag/TiO2 nanocomposites (NCs) were analyzed using different instrumental techniques. Transmission electron microscopy reveals the Ag NPs have uniformly distribute...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12983

    authors: Deshmukh SP,Mullani SB,Koli VB,Patil SM,Kasabe PJ,Dandge PB,Pawar SA,Delekar SD

    更新日期:2018-11-01 00:00:00

  • Photoaffinity labeling of the N-methyltransferase domains of cyclosporin synthetase.

    abstract::The multifunctional polypeptide cyclosporin synthetase (CySyn) remains one of the most complex nonribosomal peptide synthetase described. In this study we used a highly specific photoaffinity labeling procedure with the natural cofactor S-adenosyl-L-methionine (AdoMet), 14C-isotopically labeled at the Sdelta methyl gr...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)077<0129:plotnm>2.0.co;2

    authors: Velkov T,Lawen A

    更新日期:2003-02-01 00:00:00

  • Fluence Rate Determines PDT Efficiency in Breast Cancer Cells Displaying Different GSH Levels.

    abstract::Photodynamic therapy (PDT) appears as a promising alternative in the treatment of breast cancer since it can be highly effective in curing cancer while preserving normal tissue. However, predicting outcomes in PDT still constitutes a great challenge. One of the parameters that are usually empirically determined is the...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13182

    authors: Dos Santos AF,de Almeida DRQ,Terra LF,Wailemann RAM,Gomes VM,Arini GS,Ravagnani FG,Baptista MS,Labriola L

    更新日期:2020-05-01 00:00:00

  • Bringing It All Together: Coupling Excision Repair to the DNA Damage Checkpoint.

    abstract::Nucleotide excision repair and the ATR-mediated DNA damage checkpoint are two critical cellular responses to the genotoxic stress induced by ultraviolet (UV) light and are important for cancer prevention. In vivo genetic data indicate that these global responses are coupled. Aziz Sancar et al. developed an in vitro co...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12667

    authors: Lindsey-Boltz LA

    更新日期:2017-01-01 00:00:00

  • Metformin, an antidiabetic agent reduces growth of cutaneous squamous cell carcinoma by targeting mTOR signaling pathway.

    abstract::The biguanide metformin is widely used for the treatment of Type-II diabetes. Its antiproliferative and pro-apoptotic effects in various tumor cells suggest its potential candidacy for cancer chemoprevention. Herein, we report that metformin significantly inhibited human epidermoid A431 tumor xenograft growth in nu/nu...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01165.x

    authors: Chaudhary SC,Kurundkar D,Elmets CA,Kopelovich L,Athar M

    更新日期:2012-09-01 00:00:00

  • Photoactivation of hypericin generates singlet oxygen in mitochondria and inhibits succinoxidase.

    abstract::Photosensitized inhibition of mitochondrial succinoxidase by hypericin was measured in vitro and found to be drug-dose, light-dose, and wavelength dependent. Singlet oxygen generation, monitored using the singlet oxygen trap tetramethylethylene, and oxygen consumption in isolated mitochondria sensitized by hypericin w...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1992.tb04208.x

    authors: Thomas C,MacGill RS,Miller GC,Pardini RS

    更新日期:1992-01-01 00:00:00

  • Immunological and biochemical evidence that blepharismin is not a prosthetic group.

    abstract::A polyclonal, multispecific antiserum was raised against a whole 3[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate-extract of nonirradiated Blepharisma japonicum cells. It was used to reveal the composition of solutions that were hypothesized to contain the photoreceptor of the ciliate. A Bio-Gel A 1.5 m fine ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0669:iabetb>2.0.co;2

    authors: Podestà A,Gioffré D,Grossi T,Montagnoli G

    更新日期:2000-05-01 00:00:00

  • Correlations between reported and measured ultraviolet radiation exposure of mothers and young children.

    abstract::Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0060:cbramu>2.0.co;2

    authors: O'Riordan DL,Stanton WR,Eyeson-Annan M,Gies P,Roy C

    更新日期:2000-01-01 00:00:00

  • Cyanine Modification Tuned for Amine Photorelease.

    abstract::Cyanines are emerging as useful agents for photoreleasing biological compounds because of their capability of utilizing near-infrared (NIR) light. Another benefit is their ability to self-sensitize to produce singlet oxygen for the release of aryl amines, a process that has not been as feasible in the past. Here, we h...

    journal_title:Photochemistry and photobiology

    pub_type: 评论,杂志文章

    doi:10.1111/php.13102

    authors: Mathew JP,Greer A

    更新日期:2019-09-01 00:00:00

  • Melanocortin 1 receptor variants: functional role and pigmentary associations.

    abstract::The significance of human cutaneous pigmentation lies in its protective role against sun-induced DNA damage and photocarcinogenesis. Fair skin and red hair are characterized by a low eumelanin to pheomelanin ratio, and have been associated with increased risk of skin cancer. Cutaneous pigmentation is a complex genetic...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1751-1097.2011.00970.x

    authors: Dessinioti C,Antoniou C,Katsambas A,Stratigos AJ

    更新日期:2011-09-01 00:00:00

  • Energy dissipation processes of singlet-excited 1-hydroxyfluorenone and its hydrogen-bonded complex with N-methylimidazole.

    abstract::Effects of solvent, pH and hydrogen bonding with N-methylimidazole (MIm) on the photophysical properties of 1-hydroxyfluorenone (1HOF) have been studied. Fluorescence lifetime, fluorescence quantum yield and triplet yield measurements demonstrated that intersystem crossing was the dominant process in apolar media and ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2004-01-27RA-067.1

    authors: Sebok-Nagy K,Biczók L,Morimoto A,Shimada T,Inoue H

    更新日期:2004-07-01 00:00:00

  • Photochemical Immobilization of Polymers on a Surface: Controlling Film Thickness and Wettability.

    abstract::In this manuscript, we demonstrate the control of film thickness and surface wettability in the photochemical immobilization of poly (vinyl alcohol) (PVA) to a self-assembled monolayer (SAM) containing a phthalimide chromophore. Surface attachment is characterized by ellipsometry and contact angle measurements. The we...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12751

    authors: Carroll GT,Turro NJ,Mammana A,Koberstein JT

    更新日期:2017-10-01 00:00:00

  • Characterization of psaI and psaL mutants of Synechococcus sp. strain PCC 7002: a new model for state transitions in cyanobacteria.

    abstract::The psaI and psaL genes were characterized from the cyanobacterium Synechococcus sp. strain PCC 7002. The gene organization was different from that reported for other cyanobacteria with psaI occurring upstream and being divergently transcribed from the psaL gene. Mutants lacking PsaI or PsaL were generated by interpos...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1996.tb02421.x

    authors: Schluchter WM,Shen G,Zhao J,Bryant DA

    更新日期:1996-07-01 00:00:00

  • Phototoxicity of some bromine-substituted rhodamine dyes: synthesis, photophysical properties and application as photosensitizers.

    abstract::The synthesis of some bromine-substituted rhodamine derivatives viz., 4,5-dibromorhodamine methyl ester (dye 2) and 4,5-dibromorhodamine n-butyl ester (dye 3) are reported. These dyes were synthesized to promote a more efficient cancer cell photosensitizer for potential use in in vitro bone marrow purging in preparati...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1996.tb03008.x

    authors: Pal P,Zeng H,Durocher G,Girard D,Li T,Gupta AK,Giasson R,Blanchard L,Gaboury L,Balassy A,Turmel C,Laperrière A,Villeneuve L

    更新日期:1996-02-01 00:00:00

  • Evaluation of Photoprotective Potential and Percutaneous Penetration by Photoacoustic Spectroscopy of the Schinus terebinthifolius Raddi Extract.

    abstract::Schinus terebinthifolius is a plant rich in phenolic compounds, which have antioxidant properties and can provide new opportunities for treatment and prevention of diseases mediated by ultraviolet radiation like photoaging and skin cancer. The aim of this study was to evaluate the photoprotective potential and ex vivo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12419

    authors: Bulla MK,Hernandes L,Baesso ML,Nogueira AC,Bento AC,Bortoluzzi BB,Serra LZ,Cortez DA

    更新日期:2015-05-01 00:00:00

  • Phototaxis of Haloarcula marismortui revealed through a novel microbial motion analysis algorithm.

    abstract::Haloarcula marismortui has been described to be nonmotile prior to the recent identification of flagellar filaments, suggesting the motile nature of H. marismortui. Here we observed the locomotion of freshly cultured H. marismortui cells and tracked the swimming trajectories via ImageJ. Trajectories of H. marismortui ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2010.00768.x

    authors: Lin YC,Fu HY,Yang CS

    更新日期:2010-09-01 00:00:00

  • Chlorin e6 derivative radachlorin mainly accumulates in mitochondria, lysosome and endoplasmic reticulum and shows high affinity toward tumors in nude mice in photodynamic therapy.

    abstract::The efficacy of photodynamic therapy (PDT) depends upon the amount of photosensitizer accumulated in the malignant tissues. Radachlorin is a popular photosensitizer used in photodynamic therapy to treat various types of cancer. In this study, we have studied the main organelles responsible for the accumulation of rada...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12273

    authors: Biswas R,Moon JH,Ahn JC

    更新日期:2014-09-01 00:00:00

  • Activation of the General Stress Response of Bacillus subtilis by Visible Light.

    abstract::A key challenge for microbiology is to understand how evolution has shaped the wiring of regulatory networks. This is amplified by the paucity of information of power-spectra of physicochemical stimuli to which microorganisms are exposed. Future studies of genome evolution, driven by altered stimulus regimes, will the...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12499

    authors: van der Steen JB,Hellingwerf KJ

    更新日期:2015-09-01 00:00:00

  • Diversity of Chlamydomonas channelrhodopsins.

    abstract::Channelrhodopsins act as photoreceptors for control of motility behavior in flagellates and are widely used as genetically targeted tools to optically manipulate the membrane potential of specific cell populations ("optogenetics"). The first two channelrhodopsins were obtained from the model organism Chlamydomonas rei...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2011.01027.x

    authors: Hou SY,Govorunova EG,Ntefidou M,Lane CE,Spudich EN,Sineshchekov OA,Spudich JL

    更新日期:2012-01-01 00:00:00

  • Substituent effects on the photophysical properties of pterin derivatives in acidic and alkaline aqueous solutions.

    abstract::Pterins are heterocyclic compounds with important biological functions, and most of them may exist in two acid-base forms in the pH range between 3 and 13 in aqueous solution. In this work, the photophysical properties of acid and basic forms of six compounds of the pterin family (6-hydroxymethylpterin [HPT], 6-methyl...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-05-10-RA-522

    authors: Cabrerizo FM,Petroselli G,Lorente C,Capparelli AL,Thomas AH,Braun AM,Oliveros E

    更新日期:2005-09-01 00:00:00

  • Iodouracil as a personal dosimeter for solar UVB.

    abstract::A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar ir...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Rahn R,Lee MA

    更新日期:1998-08-01 00:00:00

  • Analysis of the ethidium bromide bound to DNA by photoacoustic and FTIR spectroscopy.

    abstract::Under physiological conditions B-form DNA is an exceedingly stable structure. However, experimental evidences obtained through nuclear magnetic resonance and fluorescence anisotropy suggest that the structure of the double helix fluctuates substantially. We describe photoacoustic phase modulation frequency measurement...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)074<0512:aotebb>2.0.co;2

    authors: Bugs MR,Cornélio ML

    更新日期:2001-10-01 00:00:00