A comparison of fluorescence methods used in the pharmacokinetic studies of Zn(II)phthalocyanine in mice.

Abstract:

:The pharmacokinetics of Zn phthalocyanine (ZnPc) encapsulated in dipalmitoyl-phosphatidylcholine (DPPC) liposomes, injected intravenously in Skh:HR-1 nude mice, was monitored by two in vitro techniques and one in vivo technique, all based on fluorescence spectroscopy. The in vitro methods involve either fluorescence measurements on thin tissue sections or on extracts from these tissues. The in vivo method involves the fluorescence measurement at the skin surface. Both in vitro techniques gave similar results which are consistent with previous findings on the pharmacokinetic behavior of ZnPc. The liver and spleen showed rapid ZnPc concentration increases, reaching a maximum level in 30 min. or less post drug administration. Relatively little ZnPc was detected in the skin, fat or muscle, the maximum concentration occurring at 12 h. In vivo fluorescence reached a maximum intensity approx. 6 h post injection at the mid-chest analysis site and at 12 h in the thigh. The in vivo measurements at two different anatomical sites showed pharmacokinetic behavior that reflects an overall integrated fluorescence originating from several tissue sites.

journal_name

Photochem Photobiol

authors

Biolo R,Jori G,Kennedy JC,Nadeau P,Pottier R,Reddi E,Weagle G

doi

10.1111/j.1751-1097.1991.tb08475.x

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

113-8

issue

1

eissn

0031-8655

issn

1751-1097

journal_volume

53

pub_type

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