Determination by fluorimetric titration of the ionization constants of ciprofloxacin in solution and in the presence of liposomes.

Abstract:

:A fluorescence titration method was applied for the determination of pKa of ciprofloxacin (CPX) in solution. Values of 6.18 +/- 0.05 and 8.76 +/- 0.03 were obtained for pKa1 and pKa2, respectively. The method was used to determine the ionization constants in the presence of liposomes of dipalmitoylphosphatidylcholine (DPPC) and DPPC with 10 mol% of dipalmitoylphosphatidylglycerol. A dependence on the surface charge of liposomes was found which supported the existence of a basic electrostatic interaction between CPX and the phospholipid bilayer. Both pK values for the N-4 butyl-piperazinyl derivative (BCPX) of the parent compound were also determined in solution and in the presence of liposomes. The competition of both drugs for the same binding site as 1-anilino-8-naphtalene sulfonate demonstrate that the interaction is governed by electrostatic forces.

journal_name

Photochem Photobiol

authors

Vázquez JL,Berlanga M,Merino S,Domènech O,Viñas M,Montero MT,Hernández-Borrell J

doi

10.1562/0031-8655(2001)073<0014:dbftot>2.0.co;2

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

14-9

issue

1

eissn

0031-8655

issn

1751-1097

journal_volume

73

pub_type

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