Abstract:
:The psaI and psaL genes were characterized from the cyanobacterium Synechococcus sp. strain PCC 7002. The gene organization was different from that reported for other cyanobacteria with psaI occurring upstream and being divergently transcribed from the psaL gene. Mutants lacking PsaI or PsaL were generated by interposon mutagenesis and characterized physiologically and biochemically. Mutant strains PR6307 (delta psaI), PR6308 (psaI-) and PR6309 (psaL-) had doubling times similar to that of the wild type under both high- and low-intensity white light, but all grew more slowly than the wild type in green light. Only monomeric photosystem I (PS I) complexes could be isolated from each mutant strain when Triton X-100 was used to solubilize thylakoid membranes; however, approximately 10% of the PS I complexes from the psaI mutants, but not the psaL mutant, could be isolated as trimers when n-dodecyl beta-D-maltoside was used. Compositional analyses of the mutant PS I complexes indicate that the presence of PsaL is required for trimer formation or stabilization and that PsaI plays a role in stabilizing the binding of both PsaL and PsaM to the PS I complex. Strain PR6309 (psaL-) was capable of performing a state 2 to state 1 transition approximately three times more rapidly than the wild type. Because the monomeric PS I complexes of this mutant should be capable of diffusing more rapidly than trimeric complexes, these data suggest that PS I complexes rather than phycobilisomes might move during state transitions. A "mobile-PS I" model for state transitions that incorporates these ideas is discussed.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Schluchter WM,Shen G,Zhao J,Bryant DAdoi
10.1111/j.1751-1097.1996.tb02421.xsubject
Has Abstractpub_date
1996-07-01 00:00:00pages
53-66issue
1eissn
0031-8655issn
1751-1097journal_volume
64pub_type
杂志文章abstract::Photoexcitation to generate triplet state has been proved to be the main photoreaction in homogeneous system for many benzoquinone derivatives, including oxidized coenzyme Q (CoQ) and its analogs. In the present study, microemulsion of CoQ, a heterogeneous system, is employed to mimic the distribution of CoQ in biomem...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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abstract::Limited trypsinization of large pea (Pisum sativum cv. Alaska) phytochrome and subsequent size-exclusion chromatography (SEC) in 0.1 M Na phosphate, pH 7.8, yielded a high-molecular-mass aggregate of tryptic fragments of phytochrome. Further SEC in 0.1 M Tris-HCl, pH 7.5, plus various concentrations of NaSCN, indicate...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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doi:
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
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更新日期:2009-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12743
更新日期:2017-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12044
更新日期:2013-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1562/fr-03-15.1
更新日期:2004-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1989-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12531
更新日期:2015-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2007.00289.x
更新日期:2008-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1751-1097.2007.00155.x
更新日期:2007-07-01 00:00:00
abstract::Solid-state NMR spectroscopy gives a powerful avenue for investigating G protein-coupled receptors and other integral membrane proteins in a native-like environment. This article reviews the use of solid-state (2)H NMR to study the retinal cofactor of rhodopsin in the dark state as well as the meta I and meta II photo...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.2008.00510.x
更新日期:2009-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13390
更新日期:2021-02-02 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13301
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pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:2000-08-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb05277.x
更新日期:1995-08-01 00:00:00
abstract::The wavelength dependence of an ultraviolet irradiation of the DNA substrate for a human endonuclease was determined. Sites of DNA incision for all UVB and UVC wavelengths examined were at cytosines which were neither cyclobutane pyrimidine dimers nor 6-4'-(pyrimidin-2-one)pyrimidines. The optimal wavelengths for form...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1989-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1991-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.13007
更新日期:2019-01-01 00:00:00
abstract::This paper focuses on the estimation of the UV Index (UVI) for all sky conditions using a simple analytical parameterization involving three independent variables: the solar zenith angle, the total ozone column and the clearness index. Measurements of the UVI made at Badajoz and Cáceres (Southwestern Spain) from Janua...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00860.x
更新日期:2011-03-01 00:00:00
abstract::We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb09559.x
更新日期:1993-08-01 00:00:00