Abstract:
:Recent developments in optical technologies have the potential to improve the speed and accuracy of screening and diagnosis of curable precancerous lesions and early cancer, thereby decreasing the costs of detection and management of epithelial malignancies. The development of molecular-specific contrast agents for markers of early neoplastic transformation could improve the detection and molecular characterization of premalignant lesions. In the oral cavity, epidermal growth factor receptor (EGFR) overexpression has been identified in early stages of premalignant lesions of the oral squamous cell carcinoma; therefore, real-time assessment of EGFR expression could serve as a biomarker for oral neoplasia. The purpose of our study was to develop a molecular-specific optical contrast agent targeted against EGFR for in vivo assessment of epithelial neoplasia using a monoclonal antibody and the far-red fluorescent dye, Alexa Fluor 660 streptavidin. In addition to demonstrating the specificity of the contrast agent for EGFR in cell lines, we document the ability to achieve penetration through 500 microm thick epithelial layers using multilayer tissue constructs and permeability-enhancing agents. Finally, using the fluorescence intensity of the contrast agent on fresh oral cavity tissue sections, we were able to distinguish abnormal from normal oral tissue. This contrast agent should have important clinical applications for use in conjunction with fluorescence spectroscopy or imaging (or both) to facilitate tumor detection and demarcation.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Hsu ER,Anslyn EV,Dharmawardhane S,Alizadeh-Naderi R,Aaron JS,Sokolov KV,El-Naggar AK,Gillenwater AM,Richards-Kortum RRdoi
10.1562/fr-03-15.1subject
Has Abstractpub_date
2004-03-01 00:00:00pages
272-9issue
3eissn
0031-8655issn
1751-1097journal_volume
79pub_type
杂志文章abstract::Activation of expression of the heme oxygenase (HO) gene appears to be involved in a cellular defense system in mammalian cells. We now demonstrate that while HO-1 mRNA levels are strongly inducible in dermal fibroblasts they are barely inducible in human epidermal keratinocytes following oxidative stress (UVA radiati...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb03973.x
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1997.tb03218.x
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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更新日期:1993-10-01 00:00:00
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journal_title:Photochemistry and photobiology
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doi:
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