Abstract:
:We report the preparation of aminopropyl-functionalized magnesium phyllosilicate (Mg-organoclay) conjugated to fluorescein isothiocyanate (FITC) functionality (FITC-organoclay) by one-pot sol-gel synthesis. The physical characteristics of the Mg- and FITC-organoclays were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), FT-IR and UV-Vis spectrophotometry. X-ray fluorescence and elemental analysis were conducted to confirm the composition of the Mg-organoclay. The FITC-organoclay particles were polydispersed with an average particle size of approximately 50 nm, as determined from SEM images. The XRD patterns of FITC-organoclay exhibited broad peaks and reduced basal d(001) indicating a less condensed and more disordered structure than was observed for Mg-organoclay. The conjugation between FITC- and Mg-organoclay was characterized by FT-IR spectroscopy. Fluorescence excitation and emission spectral data demonstrated the successful conjugation of FITC dye molecules to the Mg-organoclay. The time-resolved fluorescence measurements revealed that FITC had a lifetime of 4.58 ns, whereas the lifetime of FITC-organoclay required a double exponential fit (tau(1,2) = 0.72 and 2.68 ns). As a result, the lifetime of the FITC-organoclay was shorter than that of FITC in ethanol and indicated moderate photostability in the solution state. The cellular uptake of FITC-organoclay in human lung alveolar carcinoma epithelial cells (A549) was quantified and characterized.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Lee YC,Lee TH,Han HK,Go WJ,Yang JW,Shin HJdoi
10.1111/j.1751-1097.2009.00701.xsubject
Has Abstractpub_date
2010-05-01 00:00:00pages
520-7issue
3eissn
0031-8655issn
1751-1097pii
PHP701journal_volume
86pub_type
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