Photochemically induced binding of Rh(phen)2Cl2+ to DNA.

Abstract:

:The photoinduced covalent binding of the title compound to native and heat denatured DNA is described. The level of binding has been measured by UV (for DNA) and atomic absorption (for Rh) analysis. Quantum efficiencies of 6.4 x 10(-4) mol Rh per mol photons and 1.6 x 10(-3) mol Rh per mol photons have been determined for binding to native and denatured calf thymus DNA, respectively. Levels of bound rhodium as high as 1 molecule per five bases have been achieved. There is no binding of the complex in the absence of light, and there is evidence that at least a portion of the binding may be due to the photolytic conversion of the complex into one or more stable intermediates. Studies with polyribonucleotides indicate a strong preference for binding to the purine bases.

journal_name

Photochem Photobiol

authors

Mahnken RE,Bina M,Deibel RM,Luebke K,Morrison H

doi

10.1111/j.1751-1097.1989.tb09204.x

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

519-22

issue

4

eissn

0031-8655

issn

1751-1097

journal_volume

49

pub_type

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