UV induced (6-4) photoproducts are distributed differently than cyclobutane dimers in nucleosomes.

Abstract:

:We have compared the distributions of two stable UV photoproducts in nucleosome core DNA at the single-nucleotide level using a T4 polymerase-exonuclease mapping procedure. The distribution of pyrimidine-pyrimidone (6-4) dimers was uncovered by reversing the major UV photo-product, cis-syn cyclobutane pyrimidine dimer, with E. coli DNA photolyase and photoreactivating light. Whereas the distribution of total UV photoproducts in nucleosome core DNA forms a striking 10.3 base periodic pattern, the distribution of (6-4) dimers is much more random throughout the nucleosome core domain. Therefore, histone-DNA interactions in nucleosomes strongly modulate formation of the major class of UV-induced photoproducts, while having either a constant effect or no effect on (6-4) dimer formation.

journal_name

Photochem Photobiol

authors

Gale JM,Smerdon MJ

doi

10.1111/j.1751-1097.1990.tb01732.x

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

411-7

issue

4

eissn

0031-8655

issn

1751-1097

journal_volume

51

pub_type

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