Abstract：
:Color variants of Ca2+ -regulated photoprotein obelin were shown to be an important tool for dual-analyte binding assay. To provide site-directed conjugation with biospecific molecules, several obelin color mutants carrying unique cysteine residues were obtained and characterized for their novel properties. A pair of obelins Y138F,A5C and W92F,H22E,D12C was found to be most suitable (in terms of high bioluminescent activity and stability) as reporters in simultaneous assay of two targets in a sample. Availability of SH-groups, accessible for chemical modification, essentially simplifies the synthesis of biospecific conjugates, increases their yield and conserves obelins' bioluminescence activity. Conjugates with immunoglobulin and oligonucleotide were produced and successfully applied in single nucleotide polymorphism genotyping.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Krasitskaya VV,Burakova LP,Komarova AA,Bashmakova EE,Frank LAdoi
10.1111/php.12712subject
Has Abstractpub_date
2017-03-01 00:00:00pages
553-557issue
2eissn
0031-8655issn
1751-1097journal_volume
93pub_type
杂志文章,评审abstract：:Efficient phagocytosis of photoreceptor outer segments (POS) membranes by retinal pigment epithelium (RPE) plays a key role in biological renewal of these highly peroxidizable structures. Here, we tested whether photodynamic treatment, mediated by merocyanine 540 (MC 540), rose Bengal or a zinc-substituted chlorophyll...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00727.x
更新日期：2010-07-01 00:00:00
abstract：:In this manuscript, we demonstrate the control of film thickness and surface wettability in the photochemical immobilization of poly (vinyl alcohol) (PVA) to a self-assembled monolayer (SAM) containing a phthalimide chromophore. Surface attachment is characterized by ellipsometry and contact angle measurements. The we...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12751
更新日期：2017-10-01 00:00:00
abstract：:Molecular cloning techniques were employed to obtain hybrid luciferases with their alpha and beta subunits encoded by luxA and luxB genes, respectively, from Xenorhabdus luminescens strain HW or Vibrio fischeri. Although the two wild-type luminous bacteria are phylogenetically diverged, the hybrid luciferase Xf compri...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02943.x
更新日期：1993-04-01 00:00:00
abstract：:Exploration of environmental dynamics using intrinsic biological probe tryptophan is very important; however, it suffers from various difficulties. An alternative probe, kynurenine (KN), has been found to be an efficient probe for the ultrafast dynamics in the biological environment (Goswami et al., [2010] J. Phys. Ch...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01007.x
更新日期：2012-01-01 00:00:00
abstract：:We compared mutagenesis and sister chromatid exchange (SCE) induction by 193 nm and 308 nm pulsed excimer laser radiation with 254 nm low intensity continuous wave UV light in Chinese hamster ovary (CHO) cells in culture. The 254 nm radiation was most mutagenic of the radiations, in accordance with expectation, and al...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb09188.x
更新日期：1989-04-01 00:00:00
abstract：:In recent years the class of known fluorescent proteins (FPs) has dramatically expanded as an ever-increasing numbers of variants and homologs of the green fluorescent protein (GFP) from Aequorea jellyfish have been either engineered in the lab or discovered in other marine organisms. The red fluorescent protein (RFP)...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00206.x
更新日期：2008-01-01 00:00:00
abstract：:High-altitude Andean lakes (HAAL) are a treasure chest for microbiological research in South America. Their indigenous microbial communities are exposed to extremely high UV irradiation and to multiple chemical extremes (Arsenic, high salt content, alkalinity). Microbes are found both, free-living or associated into m...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.12555
更新日期：2016-01-01 00:00:00
abstract：:Although strong fluorescence makes the R-phycoerythrin (R-PE) proteins increasingly useful in biological and clinical assays, they are subject to nonlinear effects including transitions to collective dark states and photodegradation, which complicate quantitative applications. We report measurements of R-PE fluorescen...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-05-26-RA-544
更新日期：2006-05-01 00:00:00
abstract：:Dermatophytes are fungi that can cause infections (known as tinea) of the skin, hair and nails because of their ability to use keratin. Superficial mycoses are probably the most prevalent of infectious diseases worldwide. One of the most distinct limitations of the current therapeutic options is the recurrence of the ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)077<0556:PIOTDT>2.0.CO;2
更新日期：2003-05-01 00:00:00
abstract：:The two-photon photolysis of liquid CCl4 with 25 ps pulses of 266 nm light has been studied and compared with similar studies with high energy radiation. Both neutral and ionic species are produced from excited states and ionization. The emphasis of the study is on the ionic processes, while some data related to excit...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-06-03-RA-563
更新日期：2006-01-01 00:00:00
abstract：:Photolysis of 6-chloro-1,3-dimethyluracil and mesitylene in the presence of trifluoroacetic acid (TFA) at low temperature gave 1,3,5,7,9- and 1,3,6,8,10-pentamethylcyclooctapyrimidine-2,4-diones (1b, 1c). Sequential photoreaction of the former (1b) resulted in the formation of 9,11-diazapentacyclo[6.4.0.0(1,3).0(2,5)....
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)074<0385:eropdr>2.0.co;2
更新日期：2001-09-01 00:00:00
abstract：:The total ozone column (TOC) is the most significant quantity for estimating the erythemally effective UV radiation under clear sky conditions. Uncertainties in TOC measurements and a limited spatial and temporal resolution therefore influence the quality of calculated erythemally effective radiation. The UV Index, th...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00285.x
更新日期：2008-09-01 00:00:00
abstract：:Solar ultraviolet B (UVB) radiation has been shown to induce inflammation, DNA damage, p53 mutations and alterations in signaling pathways eventually leading to skin cancer. In this study, we investigated whether fisetin reduces inflammatory responses and modulates PI3K/AKT/NFκB cell survival signaling pathways in UVB...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12337
更新日期：2015-01-01 00:00:00
abstract：:We recently reported that variations in cellular phototoxicity among a series of alkynyl-substituted zinc trisulfophthalocyanines (ZnPcS3Cn) correlates with their hydrophobicity, with the most amphiphilic derivatives showing the highest cell uptake and phototoxicity. In this study we address the role of the plasma mem...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-12-13-RA-752
更新日期：2006-11-01 00:00:00
abstract：:There is a need to improve the selectivity of photodynamic therapy and for better targeting of tumor cells within specific tumor compartments. Selective in vitro phototoxicity of a human bladder carcinoma cell line 647V has been achieved by targeting sulfonated aluminum phthalocyanines (AlSPc) with monoclonal antibodi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05139.x
更新日期：1994-11-01 00:00:00
abstract：:Inflammatory stimuli result in the production of cutaneous eicosanoids, which are known to contribute to the process of tumor promotion. Cyclooxygenase (COX), the rate-limiting enzyme for the production of prostaglandins (PG) from arachidonic acid, exists in at least two isoforms, COX-1 and COX-2. COX-1 is constitutiv...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0073:ceimah>2.0.co;2
更新日期：2002-07-01 00:00:00
abstract：:Structure-activity relationships have been widely reported for porphyrin and phthalocyanine photosensitizers, but not for phenothiazinium derivatives. Here, four phenothiazinium salts (methylene blue, toluidine blue O, 1,9-dimethyl methylene blue and the pentacyclic derivative DO15) were used to investigate how the ab...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12264
更新日期：2014-07-01 00:00:00
abstract：:The photophysical properties of two energy-transfer dyads that are potential candidates for near-infrared (NIR) imaging probes are investigated as a function of solvent polarity. The dyads (FbC-FbB and ZnC-FbB) contain either a free base (Fb) or zinc (Zn) chlorin (C) as the energy donor and a free base bacteriochlorin...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00532.x
更新日期：2009-07-01 00:00:00
abstract：:Cultured cells accumulate acridine orange (AO), which is a weak basic dye and a photosensitizer, in lysosomes and other acidic compartments. During exposure to blue light, AO-loaded macrophages show decreasing red granular fluorescence and increasing green diffuse fluorescence. This is hypothesized to represent peroxi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01685.x
更新日期：1990-01-01 00:00:00
abstract：:In aqueous solution, amphiphilic ionenes such as the [3,22]-ionene spontaneously adopt globular conformations and form microdomains that are highly micelle-like, i.e. are capable of solubilizing organic molecules, binding and exchanging counterions and accelerating or inhibiting the rates of bimolecular reactions. Tim...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2006-09-09-RA-1036
更新日期：2007-05-01 00:00:00
abstract：:Although histochemical and immunohistochemical methods are the standard procedures in diagnosis of lymphoproliferative disorders, useful improvements in evidencing histopathologic manifestations can be obtained with the introduction of tissue autofluorescence analyses. We used microspectrofluorometry and a Multispectr...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0737:miamft>2.0.co;2
更新日期：2000-06-01 00:00:00
abstract：:In the present work, we study the photodynamic action of cercosporin (cerco), a naturally occurring photosensitizer, on human cancer multicellular spheroids. U87 spheroids exhibit double the uptake of cerco than T47D and T98G spheroids as shown by flow cytometry on the single cell level. Moreover, cerco is efficiently...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13257
更新日期：2020-05-01 00:00:00
abstract：:Channelrhodopsins act as photoreceptors for control of motility behavior in flagellates and are widely used as genetically targeted tools to optically manipulate the membrane potential of specific cell populations ("optogenetics"). The first two channelrhodopsins were obtained from the model organism Chlamydomonas rei...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01027.x
更新日期：2012-01-01 00:00:00
abstract：:Normal hematopoietic progenitor cells from 129S6/SvEv mice are substantially less sensitive to Merocyanine 540 (MC540)-mediated photodynamic therapy (PDT) than hematopoietic progenitors from sex- and age-matched C57BL/6 mice. When exposed to a combination of MC540 and light commonly used for the extracorporeal purging...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0810:gvitro>2.0.co;2
更新日期：2000-12-01 00:00:00
abstract：:Confocal laser microspectrofluorometric measurements on human T47D mammary tumor cells have been performed to assess the intracellular distribution of hypericin within the various cell compartments: cytoplasmic membrane, cytoplasm and nucleus. Confocal fluorescence measurements obtained from microvolumes (approximatel...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb02382.x
更新日期：1995-09-01 00:00:00
abstract：:We analyzed the photoinactivation of the membrane functions of bacteria and erythrocytes induced by xanthene dyes. The dyes tested were rose bengal, phloxine B, erythrosine B and eosin B. These dyes induced the leakage of K(+) from Staphylococcus aureus cells within minutes of photoirradiation, in the order of rose be...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01080.x
更新日期：2012-03-01 00:00:00
abstract：:The ciliate Stentor coeruleus exhibits photodispersal, that is, these cells swim away from light sources and collect in dimly lighted areas. We imaged and reconstructed the tracks of 48 Stentor to determine which swimming behaviors produced their photodispersal. We observed that their photodispersal is not due to a ch...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2004)080<0401:BPPISC>2.0.CO;2
更新日期：2004-11-01 00:00:00
abstract：:We have tested the hypothesis that exposure to ultraviolet light would inhibit T helper-1 (Th1) responses and stimulate T helper-2 (Th2) responses, and that thus in a mouse model of allergic (i.e. extrinsic) asthma (using ovalbumin [OVA] as the allergen) increased symptoms would be observed, while in a model of Th1-de...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0253:uearar>2.0.co;2
更新日期：2000-08-01 00:00:00
abstract：:Quantitative and qualitative changes in dermal collagen and elastin occur in response to chronic ultraviolet (UV) irradiation. These changes have been implicated in the genesis of the wrinkling seen in chronically irradiated, or photoaged skin. We examined the relationship between wrinkle formation and changes in derm...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb02194.x
更新日期：1992-10-01 00:00:00
abstract：:Near-UV (337 nm) photoactivation of the 5-hydroxytryptophan decarboxylation reaction producing serotonin has been observed. The photoactivation effect was investigated as a function of fluence rate and fluence, and pH. Photoactivation of decarboxylase activity was found to occur at nearly neutral pH values (low activi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb09197.x
更新日期：1989-04-01 00:00:00
