Photochemical linkage of antibodies to silicon chips.

Abstract:

:Antibodies and antigen binding fragments thereof were photochemically immobilized on surface-modified silicon chips of 5 x 5 mm size. Silicon surface-grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aqueous media was achieved on silicon chips by activating surface-grafted benzophenones. The presence of bovine serum albumin during irradiation reduced nonspecific adsorption of the immunoreagents and retained the immunoactivity of the photoimmobilized molecules.

journal_name

Photochem Photobiol

authors

Sundarababu G,Gao H,Sigrist H

doi

10.1111/j.1751-1097.1995.tb09867.x

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

540-4

issue

6

eissn

0031-8655

issn

1751-1097

journal_volume

61

pub_type

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