General purpose lamps induce polyoma DNA replication in H3 cells.

Abstract:

:We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount of UV radiation emitted by three different light sources was determined and the effects of each source on the replication of polyoma DNA was assessed. Our findings indicate that a 100 W incandescent lamp had a minimal effect on replication, whereas a 90 s exposure to a halogen lamp or a 160 W mercury vapor lamp induced replication 1.5-fold and 2-fold, respectively, in comparison with nontreated controls. We have previously shown that asynchronous polyoma replication in H3 cells involves UV-inducible cellular protein factors. Our present results indicate that these factors are also activated by exposure to commonly used lamps that emit comparable doses of UV radiation.

journal_name

Photochem Photobiol

authors

Rutberg SE,Ronai Z

doi

10.1111/j.1751-1097.1993.tb09559.x

subject

Has Abstract

pub_date

1993-08-01 00:00:00

pages

265-9

issue

2

eissn

0031-8655

issn

1751-1097

journal_volume

58

pub_type

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