Abstract:
:Solar light leads to thymine dimers that are mutagenic and primary cause of skin cancer. Here, we report absorption and synchrotron radiation circular dichroism (CD) spectra of Tn single strands with different number n of bases (n = 2-7, 10, 11) recorded after various 254 nm irradiation times. From a principal component analysis of the CD spectra, we extract fingerprint spectra of both the cyclobutane pyrimidine dimer (CPD) and the pyrimidine (6-4) pyrimidone photoadduct (64PP). Extending the CD measurements to the vacuum ultraviolet region in combination with systematic examinations of size effects is a new approach to gain insight on the dimeric photoproducts. We find a simple linear correlation between n and average number of dimers formed after 1 h of irradiation. The probability for a thymine to engage in a dimer increases from 32% for n = 2 to 41% for n = 11, which implies limited effects of terminal thymines, i.e., the reaction does not occur preferentially at the extremities of the single strands as previously stated. It is even possible to form two dimers with only two bridging thymines. Finally, experiments conducted on calf thymus DNA provided a similar signature of the photodimer, but differences are also evident.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Baggesen LM,Hoffmann SV,Nielsen SBdoi
10.1111/php.12183subject
Has Abstractpub_date
2014-01-01 00:00:00pages
99-106issue
1eissn
0031-8655issn
1751-1097journal_volume
90pub_type
杂志文章abstract::Cationic polythiophenes have been shown to be potent antimicrobial compounds due to their ability to absorb visible light and sensitize the production of reactive oxygen species (ROS) as well as their ability to selectively associate with and damage negatively charged cell envelopes. This study demonstrates the abilit...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13013
更新日期:2018-11-01 00:00:00
abstract::Photodynamic therapy (PDT) is a way of treating malignant tumors and hyperproliferative diseases. It is based on the use of photosensitizer, herein the chlorophyll a (chl a), and a light of an appropriate wavelength. The interaction of the photosensitizer (PS) with the light produces reactive oxygen species (ROS), pow...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01238.x
更新日期:2013-03-01 00:00:00
abstract::C-Phycocyanin (PC) trimers associated with linker polypeptides were isolated from the phycobilisome (PBS) rods of Synechococcus sp. PCC 7002. LXY refers to a linker polypeptide (L) having an apparent mass of Y kDa, located at position X in the phycobilisome where X can be R (rod), C (core) or RC (rod-core junction). M...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)073<0556:ssotlh>2.0.co;2
更新日期:2001-05-01 00:00:00
abstract::The proton channels of the bacteriorhodopsin (BR) proton pump contain bound water molecules. The channels connect the purple membrane surfaces with the protonated retinal Schiff base at the membrane center. Films of purple membrane equilibrated at low relative humidity display a shift of the 570 nm retinal absorbance ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)072<0714:wacgei>2.0.co;2
更新日期:2000-11-01 00:00:00
abstract::We describe here the development of monoclonal antibodies to the hapten tryptophan-riboflavin, generated by irradiation of a solution of bovine serum albumin in the presence of riboflavin. The specificity of the three obtained monoclonal antibodies, named 1E6, 5H5, 5A8 all belonging to the IgG1 isotype, was assessed b...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1996.tb09628.x
更新日期:1996-06-01 00:00:00
abstract::The two-photon photolysis of liquid CCl4 with 25 ps pulses of 266 nm light has been studied and compared with similar studies with high energy radiation. Both neutral and ionic species are produced from excited states and ionization. The emphasis of the study is on the ionic processes, while some data related to excit...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-06-03-RA-563
更新日期:2006-01-01 00:00:00
abstract::Photoactive materials based on dye molecules incorporated into thin films or bulk solids are useful for applications as photosensitization, photocatalysis, solar cell sensitization and fluorescent labeling, among others. In most cases, high concentrations of dyes are desirable to maximize light absorption. Under these...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12107
更新日期:2013-11-01 00:00:00
abstract::Many viral infections reach clinical significance in winter, when it is cold, relative humidity is lowest and vitamin D production from solar ultraviolet-B irradiation is at its nadir. Several autoimmune diseases, such as multiple sclerosis, type 1 diabetes mellitus and asthma, are linked to viral infections. Vitamin ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.2007.00266.x
更新日期:2008-03-01 00:00:00
abstract::During the maturation of red wines, the anthocyanins of grapes are transformed into pyranoanthocyanins, which possess a pyranoflavylium cation as their basic chromophore. Photophysical properties of the singlet and triplet excited states of a series of synthetic pyranoflavylium cations were determined at room temperat...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12973
更新日期:2019-01-01 00:00:00
abstract::Haloarcula marismortui has been described to be nonmotile prior to the recent identification of flagellar filaments, suggesting the motile nature of H. marismortui. Here we observed the locomotion of freshly cultured H. marismortui cells and tracked the swimming trajectories via ImageJ. Trajectories of H. marismortui ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00768.x
更新日期:2010-09-01 00:00:00
abstract::Irradiation with 365 nm light of Wi26VA4 SV40-transformed human fibroblasts cultured for 24 h in the presence of low density lipoproteins loaded with the anticancer porphyrin mixture Photofrin II resulted in a near complete inhibition of [14C]oleic acid incorporation into triacylglycerols, cholesteryl esters and phosp...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1992.tb04209.x
更新日期:1992-01-01 00:00:00
abstract::A set of linear and asymmetric coumarin-imidazole hybrid compounds having a N,N-diethylamine at 7-position and imidazole at 3-position on the coumarin were synthesized. Insertion of thiophene π-spacer between coumarin and imidazole moieties (5b, 5d) leads to redshifted absorption and emission compared to 5a and 5c. Al...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13024
更新日期:2019-05-01 00:00:00
abstract::The psaI and psaL genes were characterized from the cyanobacterium Synechococcus sp. strain PCC 7002. The gene organization was different from that reported for other cyanobacteria with psaI occurring upstream and being divergently transcribed from the psaL gene. Mutants lacking PsaI or PsaL were generated by interpos...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1996.tb02421.x
更新日期:1996-07-01 00:00:00
abstract::This article presents a facile method to prepare CdS/SiO(2) composite microspheres and their good catalytic properties. In our method, monodispersed SiO(2) particles bearing amino groups (-NH(2)) were synthesized at first and then used as carriers to load nanosized CdS particles to form CdS/SiO(2) composite microspher...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01188.x
更新日期:2012-11-01 00:00:00
abstract::This paper reviews briefly the evidence for an association between various measures of UV radiation (UVR) exposure and the development of cancer. Issues such as data quality, study design, measurement variation, comparability of studies, and quantification of UV exposure in relation to skin cancer are discussed. A ran...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/2005-02-18-IR-445
更新日期:2005-11-01 00:00:00
abstract::The temperature dependence of the uptake phase of 5-aminolevulinic acid (ALA) and the following production phase of protoporphyrin IX (PpIX) in normal mouse skin was investigated. A cream containing 20% ALA was topically applied on the skin for 10 min. The amount of ALA-induced PpIX was evaluated by measuring the fluo...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::The broad-band normal incidence UVB beam radiation has been measured at Neve Zohar, Dead Sea basin, using a prototype tracking instrument composed of a Model 501A UV-Biometer mounted on an Eppley Solar Tracker Model St-1. The diffuse and beam fraction of the solar global UVB radiation have been determined using the co...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00842.x
更新日期:2011-01-01 00:00:00
abstract::Ultraviolet-A irradiation of a suprofen (2-[4-2(2-thenoyl) phenyl]propionic acid) (SPF) buffered solution (pH 7.4) in the presence of supercoiled pBR322 DNA leads to single strand breaks with the formation of an open circular form and subsequent linearization of the plasmid. On the basis of agarose gel electrophoresis...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb05252.x
更新日期:1995-07-01 00:00:00
abstract::As our understanding of the role of UV in causing skin cancer continues to expand, researchers and clinicians must continue to remain up to date on the various means by which UV exposure can be quantified. The purpose of this article was to review the current methods used to measure lifetime exposure to UV and to summ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00367.x
更新日期:2008-11-01 00:00:00
abstract::High intensity two-photon picosecond excitation at 266 nm (effective energy 9.2 eV) has been shown to cause efficient photolysis of thymine in aqueous solution. Experimental results for the quantum yield of solvated electrons, phi (eaq-), and the net consumption of thymine, phi(-Thy) have been interpreted (Oraevsky an...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1991.tb01996.x
更新日期:1991-07-01 00:00:00
abstract::Efficacy of ionizing radiation (I/R) was compared with phototoxic effects of photodynamic therapy (PDT) in vitro using two cell lines derived from patients with head and neck squamous cell carcinoma (HNSCC). A cell line derived from a donor with a human papilloma virus (HPV) infection was more responsive to I/R but si...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13150
更新日期:2020-05-01 00:00:00
abstract::The multifunctional polypeptide cyclosporin synthetase (CySyn) remains one of the most complex nonribosomal peptide synthetase described. In this study we used a highly specific photoaffinity labeling procedure with the natural cofactor S-adenosyl-L-methionine (AdoMet), 14C-isotopically labeled at the Sdelta methyl gr...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)077<0129:plotnm>2.0.co;2
更新日期:2003-02-01 00:00:00
abstract::We examined the influence of solar ultraviolet-B radiation (UV-B; 280-320 nm) on the growth, biomass production and phenylpropanoid concentrations of Deschampsia antarctica during the springtime ozone depletion season at Palmer Station, along the Antarctic Peninsula. Treatments involved placing filters on frames over ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-09-18-RA-321
更新日期:2005-09-01 00:00:00
abstract::The excited-state intramolecular H-atom transfer reactions of hypocrellins B and A are compared by using time-resolved absorption and fluorescence upconversion techniques. The hypocrellin B photophysics are well described by a simple model involving one ground-state species and excited-state forward and reverse H-atom...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::The quenching of the benzophenone triplet by lysozyme and its constituent amino acids in aqueous solutions have been studied. Native lysozyme quenches the benzophenone triplet with a high rate constant, 4 x 10(9) M-1 s-1. The quenching process takes place with production of significant amounts of free ketyl radicals, ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb08424.x
更新日期:1989-05-01 00:00:00
abstract::Phototherapy with broadband UVB is an effective treatment for inflammatory dermatoses. A newly developed fluorescent UVB lamp (Philips TL01) that emits a narrowband UVB around 311 nm was shown to be superior for the phototherapy of psoriasis. In order to contribute to the knowledge about the carcinogenic potential of ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::The synthesis, characterization and photophysical properties of two perfluoroalkyl (5,10,15,20-tetrakis-[trifluoromethyl]- and [heptafluoropropyl]-porphyrin) and two perfluoroaryl (5,10,15,20-tetrakis-[2,6-difluorophenyl]- and [pentafluorophenyl]-porphyrin) are described, with reference to their potential in both phot...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0249:ssapos>2.0.co;2
更新日期:2002-03-01 00:00:00
abstract::We investigated the hypothesis that the strength of the activation of the intra-S DNA damage checkpoint varies within the S phase. Synchronized diploid human fibroblasts were exposed to either 0 or 2.5 J m(-2) UVC in early, mid- and late-S phase. The endpoints measured were the following: (1) radio-resistant DNA synth...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12361
更新日期:2015-01-01 00:00:00
abstract::The retinal pigment epithelium (RPE) is a highly metabolic layer of postmitotic cells lining Bruch's membrane in the retina. While these cells contain endogenous photosensitizers that mediate blue light-induced damage, it has also been shown that blue light exposure damages mitochondrial DNA in RPE cells resulting in ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12683
更新日期:2017-03-01 00:00:00
abstract::Laser flash photolysis was used to determine the kinetics of electron transfer between membrane-bound triplet chlorophyll (3C), cytochrome c (cyt c) located in the external water phase, and vesicle-reconstituted cytochrome c oxidase (CCO). 2,5-Di-t-butyl benzoquinone (2,5 TBQ) was used as an electron transfer mediator...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb08697.x
更新日期:1990-10-01 00:00:00