Assessment of DNA damage induced by broadband and narrowband UVB in cultured lymphoblasts and keratinocytes using the comet assay.

abstract：:The Escherichia coli protein YcgF contains a photosensory flavin adenine dinucleotide (FAD)-binding BLUF domain covalently linked to an EAL domain, which is predicted to have cyclic-di-guanosine monophosphate (GMP) phosphodiesterase activity. We have cloned, overexpressed and purified this protein, which we refer to a...

journal_title：Photochemistry and photobiology

authors： Rajagopal S,Key JM,Purcell EB,Boerema DJ,Moffat K

更新日期：2004-11-01 00:00:00

abstract：:Solar light leads to thymine dimers that are mutagenic and primary cause of skin cancer. Here, we report absorption and synchrotron radiation circular dichroism (CD) spectra of Tn single strands with different number n of bases (n = 2-7, 10, 11) recorded after various 254 nm irradiation times. From a principal compone...

journal_title：Photochemistry and photobiology

authors： Baggesen LM,Hoffmann SV,Nielsen SB

更新日期：2014-01-01 00:00:00

abstract：:Herein, we present a study of the pharmacokinetics and biodistribution of a butadiyne-linked conjugated porphyrin dimer (Oxdime) designed to have high near-infrared (NIR) 2-photon absorption cross-section for photodynamic therapy (PDT). Changes in biodistribution over time were monitored in mice carrying B16-F10 melan...

journal_title：Photochemistry and photobiology

authors： Khurana M,Ulrich S,Kim A,Moriyama Y,Netchev G,Akens MK,Anderson HL,Wilson BC

更新日期：2012-11-01 00:00:00

abstract：:High temperature can promote cyanobacterial blooms, whereas ultraviolet radiation (UVR) can potentially depress cyanobacterial growth by damaging their photosynthetic apparatus. Although the damaging effect of UVR has been well documented, reports on the interactive effects of UV radiation exposure and warming on cyan...

journal_title：Photochemistry and photobiology

authors： Noyma NP,Mesquita MCB,Roland F,Marinho MM,Huszar VLM,Lürling M

更新日期：2021-01-04 00:00:00

abstract：:Catalases are oxidized by singlet oxygen giving rise to more acidic conformers detected in zymograms after electrophoresis in polyacrylamide gels. This shift in catalase mobility can be indicative of singlet oxygen production in vivo. Catalase from human cells, as from many organisms, is susceptible to in vitro modifi...

journal_title：Photochemistry and photobiology

authors： Lledías F,Hansberg W

更新日期：1999-12-01 00:00:00

abstract：:Continuous red light controls starch degradation in turions of Spirodela polyrhiza [Dölger, K., U. K. Tirlapur and K.-J. Appenroth [1997] Photochem. Photobiol. 66, 126-127 (1997)]. This light could be replaced by repeated red light pulses with the reciprocity law fulfilled over a large range of fluence rates. The effe...

journal_title：Photochemistry and photobiology

authors： Appenroth KJ,Gabrys H

更新日期：2001-01-01 00:00:00

abstract：:Photosensitizer-functionalized reduced graphene oxide (rGO) nanoparticles are promising materials for photodynamic therapy in cancer management. In this study, rGO is synthesized by a green route employing glucose as the reducing agent and functionalized with photosensitizer, protoporphyrin IX (PPIX) in a convenient, ...

journal_title：Photochemistry and photobiology

authors： Shuvra Smita S,Das A,Barui A

更新日期：2020-11-01 00:00:00

abstract：:Seafarers working on decks of vessels at low latitudes are exposed to extremely high solar UV radiation. Their risk of developing skin cancer may be enhanced. Solar erythemal UV irradiance and exposure were measured for the first time on merchant vessels going along typical international routes at low latitudes. The m...

journal_title：Photochemistry and photobiology

authors： Feister U,Meyer G,Kirst U

更新日期：2013-11-01 00:00:00

abstract：:The energy and oscillator strength of electronic transitions of chlorophyll (Chl)-amino acid complexes were calculated by using molecular orbital methods. The energies varied widely with coordinated amino acids and the difference between the maximum and minimum energy was about 830 cm-1. This energy difference was com...

journal_title：Photochemistry and photobiology

authors： Nishigaki A,Ohshima S,Nakayama K,Okada M,Nagashima U

更新日期：2001-03-01 00:00:00

abstract：:An important step in predicting the effects of future increases in UV radiation (UVR) is to evaluate the mechanisms that organisms use to prevent and repair DNA damage and determine how those mechanisms influence UVR sensitivity. Damage is prevented to varying degrees through photoprotection and repaired via two main ...

journal_title：Photochemistry and photobiology

authors： Olson MH,Mitchell DL

更新日期：2006-03-01 00:00:00

abstract：:Towards our overall objectives of developing potent antimicrobial materials to combat the escalating threat to human health posed by the transmission of surface-adhering pathogenic bacteria, we have investigated the photobactericidal activity of cellulose nanocrystals that have been modified with a porphyrin-derived p...

journal_title：Photochemistry and photobiology

authors： Carpenter BL,Feese E,Sadeghifar H,Argyropoulos DS,Ghiladi RA

更新日期：2012-05-01 00:00:00

abstract：:Chlorprothixene (CPTX, Taractan) is a low potency antipsychotic mainly used for the treatment of psychotic disorders (e.g. schizophrenia) and acute mania occurring as part of bipolar disorders. As in the case of other numerous drugs used in the treatment of psychiatric disorders, CPTX presents geometric isomerism. The...

journal_title：Photochemistry and photobiology

authors： Piñero LE,García C,Lhiaubet-Vallet V,Oyola R,Miranda MA

更新日期：2009-07-01 00:00:00

abstract：:On direct photoexcitation, subpicosecond time-resolved absorption spectroscopy revealed that the 1B(u)-type singlet excited state of all-trans-lycopene in chloroform was about seven times more efficient than all-trans-beta-carotene in generating the radical cation. The time constant of radical cation generation from t...

journal_title：Photochemistry and photobiology

authors： Han RM,Wu YS,Feng J,Ai XC,Zhang JP,Skibsted LH

更新日期：2004-09-01 00:00:00

abstract：:The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity wa...

journal_title：Photochemistry and photobiology

authors： Mobilio S,Tondelli L,Capobianco M,Gia O

更新日期：1995-02-01 00:00:00

abstract：:Protoporphyrin IX dimethyl ester (PME), a dimethyl esterification of protoporphyrin IX (PpIX), exhibits higher intracellular uptake into NPC/CNE2 cells, a poorly differentiated human nasopharyngeal carcinoma, than does PpIX. Phototoxicity studies reveal PME to be a more potent photosensitizer than is PpIX, at the earl...

journal_title：Photochemistry and photobiology

authors： Yee KK,Soo KC,Bay BH,Olivo M

更新日期：2002-12-01 00:00:00

abstract：:The photoinduced covalent binding of the title compound to native and heat denatured DNA is described. The level of binding has been measured by UV (for DNA) and atomic absorption (for Rh) analysis. Quantum efficiencies of 6.4 x 10(-4) mol Rh per mol photons and 1.6 x 10(-3) mol Rh per mol photons have been determined...

journal_title：Photochemistry and photobiology

authors： Mahnken RE,Bina M,Deibel RM,Luebke K,Morrison H

更新日期：1989-04-01 00:00:00

abstract：:The photodegradation of the S(+)- and R(-)-ketoprofen (KP) enantiomers in the bovine serum albumin matrix was studied by steady-state photolysis with the use of lambda(irr) > 320 nm and transient absorption spectroscopy with lambda(exc) = 355 nm, at 1/1 and 2/1 KP/BSA molar ratios. R(-)-KP was found to be more labile ...

journal_title：Photochemistry and photobiology

authors： Monti S,Manet I,Manoli F,Morrone R,Nicolosi G,Sortino S

更新日期：2006-01-01 00:00:00

abstract：:The fluorescent pH probe carboxy-seminaphtorhodafluor-1 (C-Snarf-1) has been used for laser microspectro-fluorometric assays of intracellular pH in 3T3 mouse fibroblasts treated with hypocrellin A. These results are compared to those previously obtained with the structurally related hydroxylated polycyclic quinone, hy...

journal_title：Photochemistry and photobiology

authors： Chaloupka R,Sureau F,Kocisova E,Petrich JW

更新日期：1998-07-01 00:00:00

abstract：:We studied the effect on the intraocular pressure (IOP) and the ciliary body (CB) morphology after four consecutive contact transcleral photodynamic treatments of the ciliary body (CB-PDT) with verteporfin in pigmented rabbits. Twenty-two pigmented rabbits underwent CB-PDT (study group), performed once (six rabbits) o...

journal_title：Photochemistry and photobiology

authors： Charisis SK,Naoumidi II,Ginis HS,Detorakis ET,Tsilimbaris MK

更新日期：2010-01-01 00:00:00

abstract：:Polysulphone (PS) dosimetry has been a widely used technique for more than 30 years to quantify the erythemally effective UV dose received by anatomic sites (personal exposure). The calibration of PS dosimeters is an important issue as their spectral response is different from the erythemal action spectrum. It is perf...

journal_title：Photochemistry and photobiology

authors： Casale GR,Siani AM,Diémoz H,Kimlin MG,Colosimo A

更新日期：2012-01-01 00:00:00

abstract：:The thermodynamic parameters for the formation of the free radicals upon electron transfer quenching of the flavin triplet state (3FMN) by tryptophan and tyrosine, Delta(FR)H and Delta(FR)V, were obtained in aqueous solution by the application of laser-induced optoacoustic spectroscopy at various temperatures. The Del...

journal_title：Photochemistry and photobiology

authors： Crovetto L,Martínez-Junza V,Braslavsky SE

更新日期：2006-01-01 00:00:00

abstract：:Raman spectroscopy (RS) has potential for disease classification within the gastrointestinal tract (GI). A near-infrared (NIR) fiber-optic RS system has been developed previously. This study reports the first in vivo Raman spectra of human gastrointestinal tissues measured during routine clinical endoscopy. This was a...

journal_title：Photochemistry and photobiology

authors： Shim MG,Song LM,Marcon NE,Wilson BC

更新日期：2000-07-01 00:00:00

abstract：:Low-power lasers are commonly used in human medicine for treatment of various pathological conditions, but mechanisms of their healing effects are still poorly understood. The results of this study provide information related to these effects at the cellular level. Two different protozoan species, Euglena gracilis and...

journal_title：Photochemistry and photobiology

authors： Koutna M,Janisch R,Unucka M,Svobodnik A,Mornstein V

更新日期：2004-11-01 00:00:00

abstract：:Polyenic systems are involved in light perception of numerous living organisms. Although a π-conjugated backbone is a common feature of all polyenes, their photophysics may vary. We provide a comparative quantum mechanical study of low-lying S1 and S2 excited states in short (3-5 double bonds) symmetric all-trans line...

journal_title：Photochemistry and photobiology

authors： Dokukina I,Marian CM,Weingart O

更新日期：2017-11-01 00:00:00

abstract：:We previously reported that a low level of lysosomal photodamage potentiated the phototoxic effect of subsequent mitochondrial photodamage mediated by the benzoporphyrin derivative (BPD) in murine hepatoma 1c1c7 cells. This was attributed to release of Ca2+ from damaged lysosomes and a calpain-mediated conversion of t...

journal_title：Photochemistry and photobiology

authors： Kessel D,Reiners JJ Jr

更新日期：2017-11-01 00:00:00

abstract：:We study the fluorescence lifetime of the well-known 1-pyrene butyric acid (PBA) to assess oxygen concentrations in living cells. The behavior of the probe is first studied in water, ethanol, protein solution and liposome suspension. The Stern-Volmer plot of these solutions is linear, and the bimolecular reaction rate...

journal_title：Photochemistry and photobiology

authors： Ribou AC,Vigo J,Salmon JM

更新日期：2004-09-01 00:00:00

abstract：:We have studied the damage of alcohol dehydrogenase (ADH) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) induced by Fe++/EDTA + H2O2 in combination with UV-A (main output at 365 nm). Enzyme inactivation, formation of hydroxyl radicals (measured in the absence of enzymes), increase in protein carbonyls, oxidation ...

journal_title：Photochemistry and photobiology

authors： Hu ML,Tappel AL

更新日期：1992-09-01 00:00:00

abstract：:The UVA and UVB components of sunlight can produce three classes of bipyrimidine DNA photolesions [cyclobutane pyrimidine dimers (CPDs), pyrimidine (6-4) pyrimidone photoproducts (6-4PPs) and related Dewar valence isomers (DewarPPs)]. The UVA/UVB ratio of sunlight is high in winter and low in summer in the Northern He...

journal_title：Photochemistry and photobiology

authors： Nishimura K,Ikehata H,Douki T,Cadet J,Sugiura S,Mori T

更新日期：2020-12-03 00:00:00

abstract：:Topical 6,4,4'-trimethylangelicin (TMA) plus UVA was used to induce intense epidermal pigmentation in inbred HRA.HRII-c/+/Skh hairless pigmented mice over a 13 day period. Subsequent UVB/UVA exposure was used to assess the photoprotective properties of this tan using skin tumors as an endpoint. Comparisons were always...

journal_title：Photochemistry and photobiology

authors： Kipp C,Lewis EJ,Young AR

更新日期：1998-01-01 00:00:00

abstract：:The decay processes of the lowest excited singlet and triplet states of five heteropsoralens (HPS) were investigated by steady-state and shift-phase fluorometry and by laser-flash photolysis in different solvents. The emission spectra of HPS are detectable only in trifluoroethanol (TFE), where fluorescence lifetimes (...

journal_title：Photochemistry and photobiology

authors： Aloisi GG,Elisei F,Moro S,Miolo G,Dall'Acqua F

更新日期：2000-05-01 00:00:00