Abstract：
:An important step in predicting the effects of future increases in UV radiation (UVR) is to evaluate the mechanisms that organisms use to prevent and repair DNA damage and determine how those mechanisms influence UVR sensitivity. Damage is prevented to varying degrees through photoprotection and repaired via two main pathways: nucleotide excision repair and photoenzymatic repair. At present, little is known about the generality or similarity of these defenses among temperate freshwater fishes. We used laboratory experiments to compare UVR defense mechanisms among five freshwater fish species representing four families and three orders. Purified DNA, freeze-killed larvae and live larvae were exposed to UVB radiation for 12 h in the presence or absence of photorepair radiation. After exposure, we quantified frequencies of cyclobutane pyrimidine dimers in each exposure treatment. All five species used photoprotection and proportional decreases in dimer frequency were similar for all species. Evidence of excision repair was also found for all species but proportional decreases in photoproduct frequencies varied among species. Finally, evidence of photoenzymatic repair was found for only two of the five species.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Olson MH,Mitchell DLdoi
10.1562/2005-08-23-RA-656subject
Has Abstractpub_date
2006-03-01 00:00:00pages
606-10issue
2eissn
0031-8655issn
1751-1097pii
2005-08-23-RA-656Rjournal_volume
82pub_type
杂志文章abstract：:Stratospheric ozone depletion has caused an increase in the amount of ultraviolet-B (UV-B) radiation reaching the earth's surface. Numerous investigations have demonstrated that the effect of UV-B enhancements on plants includes reduction in grain yield, alteration in species competition, susceptibility to disease and...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-05-03-RA-156.1
更新日期：2004-07-01 00:00:00
abstract：:Patients infected with human immunodeficiency virus (HIV) have a high prevalence of UV radiation-responsive skin diseases including psoriasis, pruritus, eosinophilic folliculitis and eczemas. On the other hand, UV has been shown to suppress T cell-mediated immune responses and to induce activation and replication of H...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.1996.tb02450.x
更新日期：1996-08-01 00:00:00
abstract：:Tetracycline molecules offer several sites for peroxidative metabolism of the type known to lead to oxygen consumption and electronic excitation. Accordingly, when tetracycline and chlortetracycline were exposed to horseradish peroxidase in the presence of hydrogen peroxide, oxygen was taken up and light emission was ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb09588.x
更新日期：1993-09-01 00:00:00
abstract：:Solar light leads to thymine dimers that are mutagenic and primary cause of skin cancer. Here, we report absorption and synchrotron radiation circular dichroism (CD) spectra of Tn single strands with different number n of bases (n = 2-7, 10, 11) recorded after various 254 nm irradiation times. From a principal compone...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12183
更新日期：2014-01-01 00:00:00
abstract：:Detergent solubilized bacteriorhodopsin (BR) proteins which contain alterations made by site-directed mutagenesis (Asp-96----Asn, D96N; Asp-85----Asn, D85N; and Arg-82----Gln, R82Q) have been studied with resonance Raman spectroscopy. Raman spectra of the light-adapted (BRLA) and M species in D96N are identical to tho...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1991.tb03638.x
更新日期：1991-03-01 00:00:00
abstract：:Eumelanin (brown/black melanin) and pheomelanin (red/yellow melanin) in human hair can be quantified using chemical methods or approximated using spectrophotometric methods. Chemical methods consume greater resources, making them less attractive for epidemiological studies. This investigation sought to identify the sp...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00237.x
更新日期：2008-05-01 00:00:00
abstract：:We have studied the damage of alcohol dehydrogenase (ADH) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) induced by Fe++/EDTA + H2O2 in combination with UV-A (main output at 365 nm). Enzyme inactivation, formation of hydroxyl radicals (measured in the absence of enzymes), increase in protein carbonyls, oxidation ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb02171.x
更新日期：1992-09-01 00:00:00
abstract：:δ-Aminolevulinic acid (ALA)-induced porphyrin accumulation is widely used in the treatment of cancer, as photodynamic therapy. To clarify the mechanisms of the tumor-preferential accumulation of protoporphyrin, we examined the effect of the expression of heme-biosynthetic and -degradative enzymes on the ALA-induced ac...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.00950.x
更新日期：2011-09-01 00:00:00
abstract：:Phage T7 can be used as a biological UV dosimeter. Its reading is proportional to the inactivation rate expressed in HT7 units. To understand the influence of phage proteins on the formation of DNA UV photoproducts, cyclobutane pyrimidine dimers (CPD) and (6-4)photoproducts ((6-4)PD) were determined in T7 DNA exposed ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:The significance of human cutaneous pigmentation lies in its protective role against sun-induced DNA damage and photocarcinogenesis. Fair skin and red hair are characterized by a low eumelanin to pheomelanin ratio, and have been associated with increased risk of skin cancer. Cutaneous pigmentation is a complex genetic...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2011.00970.x
更新日期：2011-09-01 00:00:00
abstract：:Limited trypsinization of large pea (Pisum sativum cv. Alaska) phytochrome and subsequent size-exclusion chromatography (SEC) in 0.1 M Na phosphate, pH 7.8, yielded a high-molecular-mass aggregate of tryptic fragments of phytochrome. Further SEC in 0.1 M Tris-HCl, pH 7.5, plus various concentrations of NaSCN, indicate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb04136.x
更新日期：1989-07-01 00:00:00
abstract：:Photosynthetic bacteria are attractive for biotechnology because they produce no oxygen and so H2 -production is not inhibited by oxygen as occurs in oxygenic photoorganisms. Rhodopseudomonas palustris and Afifella marina containing BChl a can use irradiances from violet near-UV (VNUV) to orange (350-650 nm) light and...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12124
更新日期：2013-09-01 00:00:00
abstract：:Changes in the emission fluorescence intensity of pheophorbide-a (PHEO) in the presence of carboquone (CARBOQ) were used to obtain the association constant, the number of CARBOQ molecules interacting with PHEO, and the fluorescence quantum yield of the complex. Excitation spectra of mixtures of PHEO and CARBOQ in etha...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00869.x
更新日期：2011-03-01 00:00:00
abstract：:Pharaonis phoborhodopsin (ppR or sensory rhodopsin II) is a negative phototaxis receptor of Natronomonas pharaonis, and forms a complex, which transmits the photosignal into cytoplasm, with its cognate transducer (pHtrII). We examined a possible local dynamics change of ppR and its D75N mutant complexed with pHtrII, u...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00326.x
更新日期：2008-07-01 00:00:00
abstract：:On direct photoexcitation, subpicosecond time-resolved absorption spectroscopy revealed that the 1B(u)-type singlet excited state of all-trans-lycopene in chloroform was about seven times more efficient than all-trans-beta-carotene in generating the radical cation. The time constant of radical cation generation from t...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-04-22-RA-147
更新日期：2004-09-01 00:00:00
abstract：:In photodynamic therapy with topically applied delta-aminolevulinic acid porphyrins are acting as photosensitizers. The profile of porphyrin metabolites in normal or in neoplastic skin after administration of delta-aminolevulinic acid has not been determined in detail yet. Thus, to study porphyrin biosynthesis in huma...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb03146.x
更新日期：1997-07-01 00:00:00
abstract：:Although histochemical and immunohistochemical methods are the standard procedures in diagnosis of lymphoproliferative disorders, useful improvements in evidencing histopathologic manifestations can be obtained with the introduction of tissue autofluorescence analyses. We used microspectrofluorometry and a Multispectr...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)071<0737:miamft>2.0.co;2
更新日期：2000-06-01 00:00:00
abstract：:This review focuses on damage photosensitized by the fluoroquinolone (FQ) antibacterial drugs. Different models are employed to study biosubstrate photodamage mediated by FQs (organisms, cells, isolated biomolecules and super molecules). Being that the effect of environment (polarity of the medium, ions, pH, binding w...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2011.00978.x
更新日期：2011-11-01 00:00:00
abstract：:We have previously demonstrated the ability of UVC (254 nm) radiation to induce asynchronous polyoma replication in rat fibroblast cells (H3 line) that contain an integrated copy of polyoma virus. In the present study we show that general purpose lamps can induce polyoma replication in these cells as well. The amount ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb09559.x
更新日期：1993-08-01 00:00:00
abstract：:An approximate formula for the UV Index (UVI) under cloud-free, unpolluted, low surface albedo conditions is: UVI approximately 12.5mu(o)(2.42)(Omega/300)(-1.23) where mu(o) is the cosine of the solar zenith angle and Omega is the total vertical ozone column (in Dobson Units, DU). The dependence on mu(o) and Omega is ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00200.x
更新日期：2007-11-01 00:00:00
abstract：:Efficient phagocytosis of photoreceptor outer segments (POS) membranes by retinal pigment epithelium (RPE) plays a key role in biological renewal of these highly peroxidizable structures. Here, we tested whether photodynamic treatment, mediated by merocyanine 540 (MC 540), rose Bengal or a zinc-substituted chlorophyll...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00727.x
更新日期：2010-07-01 00:00:00
abstract：:Vitamin A (retinol), an essential human nutrient, plays an important role in cellular differentiation, regulation of epidermal cell growth and normal cell maintenance. In addition to these physiological roles, vitamin A has a rich photochemistry. Photoisomerization of vitamin A, involved in signal transduction for vis...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1562/2006-10-23-IR-1065
更新日期：2007-03-01 00:00:00
abstract：:We report here the design and synthesis of porphyrin-metallocene dyads consisting of a metallocene [either ferrocene or mixed sandwich η(5)-[C5H4(COOH)]Co(η(4)-C4Ph4) connected via an ester linkage at meso phenyl position of either free-base or zinc porphyrin. All these dyad systems were characterized by various spect...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12372
更新日期：2015-01-01 00:00:00
abstract：:We have compared the distributions of two stable UV photoproducts in nucleosome core DNA at the single-nucleotide level using a T4 polymerase-exonuclease mapping procedure. The distribution of pyrimidine-pyrimidone (6-4) dimers was uncovered by reversing the major UV photo-product, cis-syn cyclobutane pyrimidine dimer...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01732.x
更新日期：1990-04-01 00:00:00
abstract：:The photodegradation of the herbicide clomazone in the presence of S(2)O(8) (2-) or of humic substances of different origin was investigated. A value of (9.4 +/- 0.4) x 10(8) m(-1) s(-1) was measured for the bimolecular rate constant for the reaction of sulfate radicals with clomazone in flash-photolysis experiments. ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00467.x
更新日期：2009-05-01 00:00:00
abstract：:The kinetics of transients formed under photoexcitation of benzophenone (B) dissolved in three different polymers was studied by ns laser flash photolysis. These polymers were the soft rubbers poly (ethylene-co-butylene) (EB), polystyrene block-poly(ethylene-ran-butylene)-block-polystyrene (SEBS) and hard polystyrene ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12170
更新日期：2014-03-01 00:00:00
abstract：:The present work is aimed to synthesize CdTe/ZnSe core/shell quantum dots (QDs) in an easy way and to explore the possibilities of its application in in vitro imaging of chicken tissue and embryo. The QDs were prepared using microwave irradiation with different temperatures, which is a very easy and less time-consumin...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12398
更新日期：2015-03-01 00:00:00
abstract：:The individual tryptophanyl contributions to the near-ultraviolet dichroic activity of apomyoglobin in its native conformation have been resolved. This was accomplished by comparing the spectra of two classes of apomyoglobin with different aromatic residue contents and observing the effect of a specific modification o...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb09664.x
更新日期：1994-06-01 00:00:00
abstract：:The distribution of anthocyanins in grape (Vitis vinifera L.) bunches from the Sangiovese cultivar was measured nondestructively by chlorophyll fluorescence imaging using two excitation light bands at 550 and 650 nm in sequence. The pixel intensity in the derived logarithm of the fluorescence excitation ratio image wa...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00424.x
更新日期：2008-11-01 00:00:00
abstract：:Polarized steady-state fluorescence and fluorescence excitation spectra as well as time-resolved fluorescence for B-phycoerythrin (B-PE) from red algae, Porphyridium cruentum, embedded in polyvinyl stretched films were measured. The lifetimes of polarized fluorescence were analyzed using exponential components and fra...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
