Abstract：
:We have studied the damage of alcohol dehydrogenase (ADH) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) induced by Fe++/EDTA + H2O2 in combination with UV-A (main output at 365 nm). Enzyme inactivation, formation of hydroxyl radicals (measured in the absence of enzymes), increase in protein carbonyls, oxidation of sulfhydryl (SH) groups, loss of native protein fluorescence, and enhanced protease degradation were used to determine protein damage. Hydroxyl radical production was greatly enhanced by the combination of UV-A with Fe++/EDTA + H2O2. The combined treatment increased protein carbonyls but decreased native protein fluorescence and SH groups. The combined treatment caused turbidity in GAPD but not in ADH, whereas trypsin susceptibility was increased more in ADH than in GAPD. These measurements of protein oxidation correlated well with enzyme activities. Glyceraldehyde 3-phosphate dehydrogenase and dithiothreitol were most protective against such damage, while hydroxyl radical and singlet oxygen scavengers were partially effective. Superoxide dismutase had no effect. Thus, UV-A potentiation of protein damage induced by FE++/EDTA + H2O2 appeared to involve hydroxyl radicals and perhaps singlet oxygen but not superoxide radicals. The damage to proteins induced by combination of UV-A with physiological oxidants, iron ions and H2O2 may be relevant to UV-A-induced skin and tissue damage.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Hu ML,Tappel ALdoi
10.1111/j.1751-1097.1992.tb02171.xsubject
Has Abstractpub_date
1992-09-01 00:00:00pages
357-63issue
3eissn
0031-8655issn
1751-1097journal_volume
56pub_type
杂志文章abstract：:Polysulphone (PS) dosimetry has been a widely used technique for more than 30 years to quantify the erythemally effective UV dose received by anatomic sites (personal exposure). The calibration of PS dosimeters is an important issue as their spectral response is different from the erythemal action spectrum. It is perf...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01006.x
更新日期：2012-01-01 00:00:00
abstract：:Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD-MA)-PDT of MAT-LyLu cells in vitro. Cells were incubated with BPD-MA concentrations of 0.1, 0.5 or 2.5 μg mL(-1) for 2 h and t...
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pub_type： 杂志文章
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更新日期：2011-09-01 00:00:00
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doi：10.1111/php.12301
更新日期：2015-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01234.x
更新日期：2012-11-01 00:00:00
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更新日期：2006-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 评论,杂志文章
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pub_type： 杂志文章
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更新日期：2012-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1995-06-01 00:00:00
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更新日期：2001-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2006-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb09598.x
更新日期：1992-07-01 00:00:00
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更新日期：2009-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb02460.x
更新日期：1996-08-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb02330.x
更新日期：1993-03-01 00:00:00
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更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb08656.x
更新日期：1997-08-01 00:00:00
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更新日期：2001-08-01 00:00:00
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更新日期：1990-09-01 00:00:00
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更新日期：2003-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1992-12-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1992-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2006-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.13007
更新日期：2019-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
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