Sub-lethal photodynamic damage to ARPE-19 cells transiently inhibits their phagocytic activity.


:Efficient phagocytosis of photoreceptor outer segments (POS) membranes by retinal pigment epithelium (RPE) plays a key role in biological renewal of these highly peroxidizable structures. Here, we tested whether photodynamic treatment, mediated by merocyanine 540 (MC 540), rose Bengal or a zinc-substituted chlorophyllide inhibited phagocytic activity of ARPE-19 cells in vitro. Specific phagocytosis of fluorescein-5-isothiocyanate-labeled POS isolated from cow retinas and nonspecific phagocytosis of fluorescent polystyrene beads were measured by flow cytometry. Photodynamic treatment, mediated by all three photosensitizers with sub-threshold doses, induced significant inhibition of the cell-specific phagocytosis. The nonspecific phagocytosis was inhibited by photodynamic treatment mediated only by MC 540. The inhibition of phagocytosis was a reversible phenomenon and after 24 h, the photodynamically treated cells exhibited phagocytic activity that was comparable with that of untreated cells. This study provides proof of principle that sub-threshold photodynamic treatment of ARPE-19 cells with appropriate photosensitizers is a convenient experimental approach for in vitro study of the effects of oxidative stress on specific phagocytic activity of RPE cells. We postulate that oxidative damage to key components of the cell phagocytic machinery may be responsible for severe impairment of its activity, which can lead to retinal degeneration.


Photochem Photobiol


Olchawa M,Szewczyk G,Zareba M,Piłat A,Bzowska M,Mikołajczyk T,Sarna T




Has Abstract


2010-07-01 00:00:00














  • New 5-aminolevulinic acid esters--efficient protoporphyrin precursors for photodetection and photodynamic therapy.

    abstract::Photodetection (PD) and photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PPIX) accumulation are approaches to detect and treat dysplasia and early cancer in the gastrointestinal tract and in the urinary bladder. Because ALA-induced PPIX production is limited, we synthesized ALA es...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Brunner H,Hausmann F,Knuechel R

    更新日期:2003-11-01 00:00:00

  • Photooxidation of tryptophan leading to 2-aminoacetophenone--a possible reason for the untypical aging off-flavor in wine.

    abstract::2-Aminoacetophenone (AAP) was recognized as the key compound for the so-called untypical aging off-flavor (UTA) in Vitis vinifera wines. In this study, it was shown that AAP can be formed by photooxidation of free and protein-bound tryptophan (TRP) in combination with a subsequent storage in model wine. Solutions of T...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Horlacher N,Schwack W

    更新日期:2014-11-01 00:00:00

  • Contact transcleral ciliary body photodynamic therapy with verteporfin in pigmented rabbits: effect of repeated treatments.

    abstract::We studied the effect on the intraocular pressure (IOP) and the ciliary body (CB) morphology after four consecutive contact transcleral photodynamic treatments of the ciliary body (CB-PDT) with verteporfin in pigmented rabbits. Twenty-two pigmented rabbits underwent CB-PDT (study group), performed once (six rabbits) o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Charisis SK,Naoumidi II,Ginis HS,Detorakis ET,Tsilimbaris MK

    更新日期:2010-01-01 00:00:00

  • Photophysical properties of the lowest excited singlet and triplet states of thio- and seleno-psoralens

    abstract::The decay processes of the lowest excited singlet and triplet states of five heteropsoralens (HPS) were investigated by steady-state and shift-phase fluorometry and by laser-flash photolysis in different solvents. The emission spectra of HPS are detectable only in trifluoroethanol (TFE), where fluorescence lifetimes (...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Aloisi GG,Elisei F,Moro S,Miolo G,Dall'Acqua F

    更新日期:2000-05-01 00:00:00

  • Oxidation of human catalase by singlet oxygen in myeloid leukemia cells.

    abstract::Catalases are oxidized by singlet oxygen giving rise to more acidic conformers detected in zymograms after electrophoresis in polyacrylamide gels. This shift in catalase mobility can be indicative of singlet oxygen production in vivo. Catalase from human cells, as from many organisms, is susceptible to in vitro modifi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Lledías F,Hansberg W

    更新日期:1999-12-01 00:00:00

  • Low-level laser therapy and sodium diclofenac in acute inflammatory response induced by skeletal muscle trauma: effects in muscle morphology and mRNA gene expression of inflammatory markers.

    abstract::Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expres...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: de Almeida P,Lopes-Martins RÁ,Tomazoni SS,Albuquerque-Pontes GM,Santos LA,Vanin AA,Frigo L,Vieira RP,Albertini R,de Carvalho Pde T,Leal-Junior EC

    更新日期:2013-03-01 00:00:00

  • Protective Effect of BLED-exposed Conditioned Media on Cell Injury.

    abstract::Previous studies have reported that 450 nm blue light emitting diode (BLED) induces apoptosis through a mitochondria-mediated pathway in cancer cells and reduces the early stage tumor growth. This study was performed to determine the effects of BLED-irradiated cell metabolites on cell injury. Our results showed that c...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Oh PS,Kim EM,Kim M,Kim IS,Han YH,Lim S,Sohn MH,Ko MH,Jeong HJ

    更新日期:2018-05-01 00:00:00

  • Infrared radiation influence on molt and regeneration of Neohelice granulata Dana, 1851 (Grapsidae, Sesarminae).

    abstract::This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Gonzalez VC,Lena AB,Espindola WA,de Souza Votto AP,Jorge MB,de Moraes Vaz Batista Filgueira D,Bianchini A,Sérgio Varela Júnior A,Nery LE,Trindade GS

    更新日期:2009-09-01 00:00:00

  • Energy dissipation processes of singlet-excited 1-hydroxyfluorenone and its hydrogen-bonded complex with N-methylimidazole.

    abstract::Effects of solvent, pH and hydrogen bonding with N-methylimidazole (MIm) on the photophysical properties of 1-hydroxyfluorenone (1HOF) have been studied. Fluorescence lifetime, fluorescence quantum yield and triplet yield measurements demonstrated that intersystem crossing was the dominant process in apolar media and ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Sebok-Nagy K,Biczók L,Morimoto A,Shimada T,Inoue H

    更新日期:2004-07-01 00:00:00

  • Photodynamic activities and skin photosensitivity of the bis(dimethylthexylsiloxy)silicon 2,3-naphthalocyanine in mice.

    abstract::The photodynamic therapy (PDT) activity of the bis(dimethylthexylsiloxy)silicon 2,3-naphthalocyanine (SiNc 8) was evaluated against the EMT-6 tumor implanted intradermally in BALB/c mice. The SiNc 8 was formulated in aqueous emulsions based on Cremophor EL or Solutol HS 15. The formulation was shown to affect plasma c...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Brasseur N,Ouellet R,Lewis K,Potter WR,van Lier JE

    更新日期:1995-12-01 00:00:00

  • Comparative study of photodynamic properties of 13,15-N-cycloimide derivatives of chlorin p6.

    abstract::Comparative study of 13,15-[N-(2-hydroxyethyl)]cycloimide chlorin p6 (2), 13,15-(N-acetoxy)cycloimide chlorin p6 (3), 13,15-(N-hydroxy)cycloimide chlorin p6 methyl ester (4) and 13,15-(N-methoxy)cycloimide chlorin p6 methyl ester (5) together with the previously investigated 13,15-[N-(3-hydroxypropyl)]cycloimide chlor...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Feofanov A,Sharonov G,Grichine A,Karmakova T,Pljutinskaya A,Lebedeva V,Ruziyev R,Yakubovskaya R,Mironov A,Refregier M,Maurizot JC,Vigny P

    更新日期:2004-02-01 00:00:00

  • Mutation and sister chromatid exchange induction in Chinese hamster ovary (CHO) cells by pulsed excimer laser radiation at 193 nm and 308 nm and continuous UV radiation at 254 nm.

    abstract::We compared mutagenesis and sister chromatid exchange (SCE) induction by 193 nm and 308 nm pulsed excimer laser radiation with 254 nm low intensity continuous wave UV light in Chinese hamster ovary (CHO) cells in culture. The 254 nm radiation was most mutagenic of the radiations, in accordance with expectation, and al...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Rasmussen RE,Hammer-Wilson M,Berns MW

    更新日期:1989-04-01 00:00:00

  • Influence of the photostabilizer in the photoprotective effects of a formulation containing UV-filters and vitamin A.

    abstract::Vitamin A palmitate has been used in cosmetics; however, studies report that this substance shows photoreactivity that can lead to loss of safety and efficacy. On the other hand, photostabilizers have been used to increase sunscreen photostability and consequently their safety and effectiveness. Thus, this study aimed...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Benevenuto CG,Di Matteo MA,Maia Campos PM,Gaspar LR

    更新日期:2010-11-01 00:00:00

  • In vitro photodynamic effects of lysyl chlorin p6: cell survival, localization and ultrastructural changes.

    abstract::The in vitro cell survival, localization and ultrastructural changes following irradiation were examined in 9L glioma cells sensitized with a new photosensitizer, lysyl chlorin p6 (LCP). In clonogenic assays, LCP was 10-100-fold more phototoxic than photofrin II on a microgram/mL basis. Lysyl chlorin p6 uptake was blo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Leach MW,Higgins RJ,Autry SA,Boggan JE,Lee SJ,Smith KM

    更新日期:1993-11-01 00:00:00

  • Time-resolved and steady-state fluorescence spectroscopy of eumelanin and indolic polymers.

    abstract::Eumelanin plays a variety of important physiological roles in human skin. However, its structure and fundamental properties still remain poorly understood. Although the absorbance of eumelanin is broad and reveals little about its structure, a variety of techniques have revealed the presence of a disordered array of c...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Nighswander-Rempel SP,Mahadevan IB,Rubinsztein-Dunlop H,Meredith P

    更新日期:2007-11-01 00:00:00

  • Chlorin-bacteriochlorin energy-transfer dyads as prototypes for near-infrared molecular imaging probes: controlling charge-transfer and fluorescence properties in polar media.

    abstract::The photophysical properties of two energy-transfer dyads that are potential candidates for near-infrared (NIR) imaging probes are investigated as a function of solvent polarity. The dyads (FbC-FbB and ZnC-FbB) contain either a free base (Fb) or zinc (Zn) chlorin (C) as the energy donor and a free base bacteriochlorin...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Kee HL,Diers JR,Ptaszek M,Muthiah C,Fan D,Lindsey JS,Bocian DF,Holten D

    更新日期:2009-07-01 00:00:00

  • Photoactivation of hypericin generates singlet oxygen in mitochondria and inhibits succinoxidase.

    abstract::Photosensitized inhibition of mitochondrial succinoxidase by hypericin was measured in vitro and found to be drug-dose, light-dose, and wavelength dependent. Singlet oxygen generation, monitored using the singlet oxygen trap tetramethylethylene, and oxygen consumption in isolated mitochondria sensitized by hypericin w...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Thomas C,MacGill RS,Miller GC,Pardini RS

    更新日期:1992-01-01 00:00:00

  • Early alterations of actin in cultured human keratinocytes and fibroblasts exposed to long-wavelength radiations. Possible involvement in the UVA-induced perturbations of endocytotic processes.

    abstract::Exposure of cultured MRC5 human fibroblasts or NCTC 2544 human keratinocytes to mild doses of ultraviolet A (UVA: 320-400 nm) radiations markedly decreased the actin reactivity with fluorescein-labeled phalloidin. This indicates a change in the degree of polymerization of actin and thus in the organization of actin fi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Djavaheri-Mergny M,Pieraggi MT,Mazière C,Santus R,Lageron A,Salvayre R,Dubertret L,Mazière JC

    更新日期:1994-01-01 00:00:00

  • The use of wavelength-selective plastic cladding materials in horticulture: understanding of crop and fungal responses through the assessment of biological spectral weighting functions.

    abstract::Plant responses to light spectral quality can be exploited to deliver a range of agronomically desirable end points in protected crops. This can be achieved using plastics with specific spectral properties as crop covers. We have studied the responses of a range of crops to plastics that have either (a) increased tran...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Paul ND,Jacobson RJ,Taylor A,Wargent JJ,Moore JP

    更新日期:2005-09-01 00:00:00

  • Diagnostic potential of autofluorescence for an assisted intraoperative delineation of glioblastoma resection margins.

    abstract::The intrinsic autofluorescence properties of biological tissues can be affected by the occurrence of histological and biochemical alterations induced by pathological processes. In this study the potential of autofluorescence to distinguish tumor from normal tissues was investigated with the view of a real-time diagnos...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Croce AC,Fiorani S,Locatelli D,Nano R,Ceroni M,Tancioni F,Giombelli E,Benericetti E,Bottiroli G

    更新日期:2003-03-01 00:00:00

  • Ab initio quantum chemical investigation of the first steps of the photocycle of phototropin: a model study.

    abstract::Phototropin is a blue light-activated photoreceptor that plays a dominant role in the phototropism of plants. The protein contains two subunits that bind flavin mononucleotide (FMN), which are responsible for the initial steps of the light-induced reaction. It has been proposed that the photoexcited flavin molecule ad...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Neiss C,Saalfrank P

    更新日期:2003-01-01 00:00:00

  • New triruthenium clusters as photoinduced DNA-binding and cleaving agents.

    abstract::1-Hydroxybenzotriazole and 1-hydroxypyridine-2-thione were incorporated as ligands with the cluster Ru3(CO)10 (NCMe)2 to give [(mu-H)Ru3(CO)10(mu2-2,3-eta2-NNN(O)C6 H4)] and [(mu-H)Ru3(CO)9(mu2-eta1 : eta2-C5H4N(O)S)], respectively. Irradiation of these two new triruthenium metal clusters individually with 350 nm UV l...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hwu JR,Kuang-Lieh L,Yu SF,Yu LJ,Kumaresan S,Lin KJ,Tsay SC

    更新日期:2002-05-01 00:00:00

  • Time-resolved singlet oxygen phosphorescence measurements from photosensitized experiments in single cells: effects of oxygen diffusion and oxygen concentration.

    abstract::Time-resolved singlet oxygen, O(2)(a(1)Delta(g)), phosphorescence experiments have been performed in single cells upon pulsed laser irradiation of a photosensitizer incorporated into the cell. Data recorded as a function of the partial pressure of ambient oxygen to which the cell is exposed reflect apparent values for...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hatz S,Poulsen L,Ogilby PR

    更新日期:2008-09-01 00:00:00

  • Ultraviolet radiation exposure, vitamin D, and cancer.

    abstract::This paper reviews briefly the evidence for an association between various measures of UV radiation (UVR) exposure and the development of cancer. Issues such as data quality, study design, measurement variation, comparability of studies, and quantification of UV exposure in relation to skin cancer are discussed. A ran...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Berwick M,Kesler D

    更新日期:2005-11-01 00:00:00

  • Photosensitization with bacteriochlorins.

    abstract::Biophysical and photobiological properties of a group of bacteriochlorins were compared with efficacy of these products for photodynamic therapy of murine tumors. Predictive factors for selective photosensitization in vivo include affinity binding to lipoproteins greater than albumin, extinction coefficient at the wav...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Kessel D,Smith KM,Pandey RK,Shiau FY,Henderson B

    更新日期:1993-08-01 00:00:00

  • Retarded photooxidation of cyamemazine in biomimetic microenvironments.

    abstract::Cyamemazine (CMZ) is a neuroleptic drug that mediates cutaneous phototoxicity in humans. Here, the photobehavior of CMZ has been examined within α1 -acid glycoproteins, β- and γ-cyclodextrins and SDS micelles. In all these microenvironments, CMZ emission was enhanced and blue-shifted, and its lifetime was longer. Irra...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Limones-Herrero D,Pérez-Ruiz R,Jiménez MC,Miranda MA

    更新日期:2014-09-01 00:00:00

  • Wavelength-dependent quantum yield for Z----E isomerization of bilirubin complexed with human serum albumin.

    abstract::The quantum yield, phi ZE, for configurational photoisomerization (4Z,15Z----4Z,15E) of bilirubin bound non-covalently to human serum albumin was determined (at 23 +/- 2 degrees C) by laser excitation and chromatographic analysis of products. Values obtained for photoexcitation at 465 nm were about one-half those prev...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Agati G,Fusi F,Pratesi R,McDonagh AF

    更新日期:1992-02-01 00:00:00

  • Characterization of endogenous protoporphyrin IX induced by delta-aminolevulinic acid in resting and activated peripheral blood lymphocytes by four-color flow cytometry.

    abstract::Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hryhorenko EA,Rittenhouse-Diakun K,Harvey NS,Morgan J,Stewart CC,Oseroff AR

    更新日期:1998-05-01 00:00:00

  • Supramolecular cationic tetraruthenated porphyrin and light-induced decomposition of 2'-deoxyguanosine predominantly via a singlet oxygen-mediated mechanism.

    abstract::The tetraruthenated porphyrin, mu-[meso-5,10,15,20-tetra(pyridyl)porphyrin]tetrakis[bis-(bipyridine) chloride ruthenium(II)] (TRP) is a supramolecular cationic species. The aim of the present investigation was to evaluate the photodynamic properties of TRP and Zn-TRP to damage DNA with emphasis on the mechanistic aspe...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Ravanat JL,Cadet J,Araki K,Toma HE,Medeiros MH,Mascio PD

    更新日期:1998-11-01 00:00:00

  • Fluence Rate Determines PDT Efficiency in Breast Cancer Cells Displaying Different GSH Levels.

    abstract::Photodynamic therapy (PDT) appears as a promising alternative in the treatment of breast cancer since it can be highly effective in curing cancer while preserving normal tissue. However, predicting outcomes in PDT still constitutes a great challenge. One of the parameters that are usually empirically determined is the...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Dos Santos AF,de Almeida DRQ,Terra LF,Wailemann RAM,Gomes VM,Arini GS,Ravagnani FG,Baptista MS,Labriola L

    更新日期:2020-05-01 00:00:00