Quenching enhancement of the singlet excited state of pheophorbide-a by DNA in the presence of the quinone carboquone.

Abstract:

:Changes in the emission fluorescence intensity of pheophorbide-a (PHEO) in the presence of carboquone (CARBOQ) were used to obtain the association constant, the number of CARBOQ molecules interacting with PHEO, and the fluorescence quantum yield of the complex. Excitation spectra of mixtures of PHEO and CARBOQ in ethanol (EtOH) show an unresolved doublet in the red-most excitation band of PHEO, indicating the formation of a loose ground-state complex. The 1:1 CARBOQ-PHEO complex shows a higher fluorescence quantum yield in EtOH (0.41 ± 0.02) than in buffer solution (0.089 ± 0.002), which is also higher than that of the PHEO monomer (0.28). Quenching of the PHEO fluorescence by DNA nucleosides and double-stranded oligonucleotides was also observed and the bimolecular quenching rate constants were determined. The quenching rate constant increase as the oxidation potential of the DNA nucleoside increases. Larger quenching constants were obtained in the presence of CARBOQ suggesting that CARBOQ enhances DNA photo-oxidation, presumably by inhibiting the back-electron-transfer reaction from the photoreduced PHEO to the oxidized base. Thus, the enhanced DNA-base photosensitized oxidation by PHEO in the presence of CARBOQ may be related to the large extent by which this quinone covalently binds to DNA, as previously reported.

journal_name

Photochem Photobiol

authors

Díaz-Espinosa Y,Crespo-Hernández CE,Alegría AE,García C,Arce R

doi

10.1111/j.1751-1097.2010.00869.x

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

275-83

issue

2

eissn

0031-8655

issn

1751-1097

journal_volume

87

pub_type

杂志文章
  • Effect of netropsin on one-electron oxidation of duplex DNA.

    abstract::The effect of netropsin on the oxidative reactions of duplex DNA was examined. One-electron oxidation of DNA creates a radical cation that migrates through duplex DNA and reacts primarily at GG steps. Netropsin is a dication that specifically binds primarily by hydrogen bonding in the minor groove at sites that have f...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2004)080<0456:EONOOO>2.0.CO;2

    authors: Roberts LW,Schuster GB

    更新日期:2004-11-01 00:00:00

  • Effects of the menstrual cycle on the red and near-infrared optical properties of the human breast.

    abstract::Time-resolved reflectance and transmittance spectroscopy was applied to measure in vivo the absorption and transport scattering spectra of the female breast from 610 to 1010 nm. Three measurement configurations were used to probe different breast regions, and data were collected two or three times in each of the five ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Cubeddu R,D'Andrea C,Pifferi A,Taroni P,Torricelli A,Valentini G

    更新日期:2000-09-01 00:00:00

  • Casting Away the Shadows: Elucidating the Role of Light-mediated Posttranscriptional Control in Plants.

    abstract::Light signals trigger precise changes in gene expression networks that activate distinctive developmental programs in plants. The transcriptome is shaped at different stages, both by the regulation of gene expression and also by posttranscriptional mechanisms that alter the sequence or abundance of the transcripts gen...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12762

    authors: Hernando CE,Garcia C,Mateos JL

    更新日期:2017-05-01 00:00:00

  • Effects of Combined Lysosomal and Mitochondrial Photodamage in a Non-small-Cell Lung Cancer Cell Line: The Role of Paraptosis.

    abstract::We previously reported that a low level of lysosomal photodamage potentiated the phototoxic effect of subsequent mitochondrial photodamage mediated by the benzoporphyrin derivative (BPD) in murine hepatoma 1c1c7 cells. This was attributed to release of Ca2+ from damaged lysosomes and a calpain-mediated conversion of t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12805

    authors: Kessel D,Reiners JJ Jr

    更新日期:2017-11-01 00:00:00

  • Chlorophyll fluorescence imaging for the noninvasive assessment of anthocyanins in whole grape (Vitis vinifera L.) bunches.

    abstract::The distribution of anthocyanins in grape (Vitis vinifera L.) bunches from the Sangiovese cultivar was measured nondestructively by chlorophyll fluorescence imaging using two excitation light bands at 550 and 650 nm in sequence. The pixel intensity in the derived logarithm of the fluorescence excitation ratio image wa...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00424.x

    authors: Agati G,Traversi ML,Cerovic ZG

    更新日期:2008-11-01 00:00:00

  • Characterization of the Blue-Light-Activated Adenylyl Cyclase mPAC by Flash Photolysis and FTIR Spectroscopy.

    abstract::The recently discovered photo-activated adenylyl cyclase (mPAC from Microcoleus chthonoplastes) is the first PAC that owes a light-, oxygen- and voltage-sensitive (LOV) domain for blue-light sensing. The photoreaction of the mPAC receptor was studied by time-resolved UV/vis and light-induced Fourier transform infrared...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12746

    authors: Kerruth S,Langner P,Raffelberg S,Gärtner W,Heberle J

    更新日期:2017-05-01 00:00:00

  • Dependence of fibroblast autofluorescence properties on normal and transformed conditions. Role of the metabolic activity.

    abstract::The dependence of autofluorescence properties on the metabolic and functional engagement and on the transformation condition was studied on single cells. Normal Galliera rat fibroblasts at low subculture passage (cell strain), at high subculture passage (stabilized cell line), and transformed cell line derived from a ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(1999)069<0364:dofapo>2.3.co;2

    authors: Croce AC,Spano A,Locatelli D,Barni S,Sciola L,Bottiroli G

    更新日期:1999-03-01 00:00:00

  • Photochemical and Photodynamical Properties of Sulfur-Substituted Nucleic Acid Bases.

    abstract::Sulfur-substituted nucleobases (a.k.a., thiobases) are among the world's leading prescriptions for chemotherapy and immunosuppression. Long-term treatment with azathioprine, 6-mercaptopurine and 6-thioguanine has been correlated with the photoinduced formation of carcinomas. Establishing an in-depth understanding of t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12975

    authors: Ashwood B,Pollum M,Crespo-Hernández CE

    更新日期:2019-01-01 00:00:00

  • Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium.

    abstract::Tissue inhibitors of metalloproteinases (TIMPs) are the major endogenous regulators of metalloproteinase activity in tissues. TIMPs are able to inhibit activity of all known matrix metalloproteinases (MMPs) and thus participate in controlling extracellular matrix synthesis and degradation. We showed previously elevate...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12612

    authors: Ardan T,Němcová L,Bohuslavová B,Klezlová A,Popelka Š,Studenovská H,Hrnčiarová E,Čejková J,Motlík J

    更新日期:2016-09-01 00:00:00

  • Lack of correlation between DNA strand breakage and p53 protein levels in human fibroblast strains exposed to ultraviolet lights.

    abstract::The contribution of DNA strand breaks accumulating in the course of nucleotide excision repair to upregulation of the p53 tumor suppressor protein was investigated in human dermal fibroblast strains after treatment with 254 nm ultraviolet (UV) light. For this purpose, fibroblast cultures were exposed to UV and incubat...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)072<0562:locbds>2.0.co;2

    authors: Enns L,Murray D,Mirzayans R

    更新日期:2000-10-01 00:00:00

  • Characterization of 3-Acetyl Chlorophyll a and 3-Acetyl Protochlorophyll a Accommodated in the B800 Binding Sites of Photosynthetic Light-Harvesting Complex 2 in the Purple Photosynthetic Bacterium Rhodoblastus acidophilus.

    abstract::We present the detailed characterization on the reconstitution of two cyclic tetrapyrrole pigments that have the same substituents but differ in the degree of hydrogenation in the macrocycles from bacteriochlorophyll (BChl) a (7,8,17,18-tetrahydroporphyrin) into the binding sites of B800 BChl a in light-harvesting com...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12919

    authors: Saga Y,Miyagi K

    更新日期:2018-07-01 00:00:00

  • 8-methoxypsoralen plus UVA induces the 72 kDa heat shock protein in organ-cultured normal human skin.

    abstract::The proteins induced by heat and other stressors, called heat shock proteins (HSP) or stress proteins, are considered to play a general role in protection from cellular injury. Exposure to UVA (320-400 nm) following application of 8-methoxypsoralen (8-MOP), termed PUVA is commonly used in the field of dermatology. In ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1993.tb04974.x

    authors: Muramatsu T,Yamashina Y,Tada H,Kobayashi N,Yamaji M,Ohno H,Shirai T,Takahashi A,Ohnishi T

    更新日期:1993-12-01 00:00:00

  • Novel water-soluble photosensitizer based on starch and containing porphyrin.

    abstract::Novel water-soluble polymeric photosensitizers (SPO) based on starch and containing porphyrin chromophores were synthesized and studied. The polymers were soluble in water and in dimethyl sulfoxide. Photophysical studies and solubilization of molecular probes proved the formation of hydrophobic, rigid microdomains in ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-03-07-RA-455

    authors: Nowakowska M,Sterzel M,Zapotoczny S

    更新日期:2005-09-01 00:00:00

  • Mechanism of lipid peroxidation photosensitized by tiaprofenic acid: product studies using linoleic acid and 1,4-cyclohexadienes as model substrates.

    abstract::A careful study of the linoleic acid hydroperoxide (LOOH) profile obtained upon peroxidation of linoleic acid (LA) photosensitized by tiaprofenic acid (TPA) and analogous ketones has been undertaken to distinguish between type-I and type-II photoperoxidation mechanisms. 1,4-Cyclohexadiene and 1,2-dimethylcyclohexa-2,5...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)073<0359:molppb>2.0.co;2

    authors: Samadi A,Martínez LA,Miranda MA,Morera IM

    更新日期:2001-04-01 00:00:00

  • Activities of the bimodal fluorescent protein produced by Photobacterium phosphoreum strain bmFP in the luciferase reaction in vitro.

    abstract::The activity of the bimodal fluorescent protein (bmFP) (lambda max, 488 and 517 nm) in the in vitro luciferase reaction has been studied. The bmFP that is produced by Photobacterium phosphoreum strain bmFP is a dimer of two homologous subunits binding four riboflavin 5'-phosphate (FMN)-myristate chromophores. The addi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0237:aotbfp>2.0.co;2

    authors: Karatani H,Konaka T

    更新日期:2000-02-01 00:00:00

  • Water-mediated spectral shifts in rhodopsin and bathorhodopsin.

    abstract::The role of water molecules in spectral tuning of proteins has been left largely unexplored. This topic is important because changing hydrogen bond patterns during the activation process may lead to spectral shifts which can be of diagnostic value for the underlying structures. Arguments put forward in this article ar...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1751-1097.2008.00499.x

    authors: Sekharan S

    更新日期:2009-03-01 00:00:00

  • Relative induction of cyclobutane dimers and cytosine photohydrates in DNA irradiated in vitro and in vivo with ultraviolet-C and ultraviolet-B light.

    abstract::SV40 DNA was irradiated in vitro and in vivo with UV-C (240-280 nm) and UV-B (280-320 nm) light, and damaged sites sensitive to digestion with Escherichia coli endonuclease III (endo III) and bacteriophage T4 endonuclease V (endo V) were quantified. The frequency of endo III-sensitive sites (primarily cytosine photohy...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1991.tb02084.x

    authors: Mitchell DL,Jen J,Cleaver JE

    更新日期:1991-11-01 00:00:00

  • Influence of Heterogeneity on the Ultrafast Photoisomerization Dynamics of Pfr in Cph1 Phytochrome.

    abstract::Photoisomerization of a protein-bound chromophore is the basis of light sensing and signaling in many photoreceptors. Phytochrome photoreceptors can be photoconverted reversibly between the Pr and Pfr states through photoisomerization of the methine bridge between rings C and D. Ground-state heterogeneity of the chrom...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12743

    authors: Stensitzki T,Yang Y,Wölke AL,Knapp EW,Hughes J,Mroginski MA,Heyne K

    更新日期:2017-05-01 00:00:00

  • Do melanoidins induced by topical 9% dihydroxyacetone sunless tanning spray inhibit vitamin d production? A pilot study.

    abstract::We report here preliminary pilot study data of the effect of sunless tanning spray with 9% [Correction added after online publication (August 24th, 2009): The concentration of Dihydroxyacetone used in the study was 9% and not 3% as previously stated] dihydroxyacetone (DHA) on 25-hydroxyvitamin D [25(OH)D] serum levels...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00574.x

    authors: Armas LA,Fusaro RM,Sayre RM,Huerter CJ,Heaney RP

    更新日期:2009-09-01 00:00:00

  • Photophysical properties of the lowest excited singlet and triplet states of thio- and seleno-psoralens

    abstract::The decay processes of the lowest excited singlet and triplet states of five heteropsoralens (HPS) were investigated by steady-state and shift-phase fluorometry and by laser-flash photolysis in different solvents. The emission spectra of HPS are detectable only in trifluoroethanol (TFE), where fluorescence lifetimes (...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0506:ppotle>2.0.co;2

    authors: Aloisi GG,Elisei F,Moro S,Miolo G,Dall'Acqua F

    更新日期:2000-05-01 00:00:00

  • Ligand-dependent interaction of ruthenium(II) polypyridyl complexes with DNA probed by emission spectroscopy.

    abstract::The nature of the interaction in buffered aqueous solution of several homo and heteroleptic ruthenium(II) polypyridyl complexes containing 2,2'-bipyridine (bpy), 2,2'-bipyrazine (bpz), 1,10-phenanthroline (phen), 4,7-diphenyl-1,10-phenanthroline (dip), 3,4,7,8-tetramethyl-1,10-phenanthroline (tmp), 1,4,5,8-tetraazaphe...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1990.tb01787.x

    authors: Kirsch-De Mesmaeker A,Orellana G,Barton JK,Turro NJ

    更新日期:1990-09-01 00:00:00

  • Analysis of compact fluorescent lights for use by patients with photosensitive conditions.

    abstract::Ultraviolet radiation (UVR) is hazardous to patients with photosensitive skin disorders, such as lupus erythematosus, xeroderma pigmentosum and skin cancer. As such, these patients are advised to minimize their exposure to UVR. Classically, this is accomplished through careful avoidance of sun exposure and artificial ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00540.x

    authors: Klein RS,Werth VP,Dowdy JC,Sayre RM

    更新日期:2009-07-01 00:00:00

  • Immobilized organic photosensitizers with versatile reactivity for various visible-light applications.

    abstract::Various photosensitizers were grafted by conventional peptide coupling methods to functionalized silica with several macroscopic shapes (powders, films) or embedded in highly transparent and microporous silica xerogel monoliths. Owing to the transparency and free-standing shape of the monoliths, the transient species ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12166

    authors: Ronzani F,Saint-Cricq P,Arzoumanian E,Pigot T,Blanc S,Oelgemöller M,Oliveros E,Richard C,Lacombe S

    更新日期:2014-03-01 00:00:00

  • Subcellular localization of hematoporphyrin derivative in bladder tumor cells in culture.

    abstract::Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1990.tb01736.x

    authors: Shulok JR,Wade MH,Lin CW

    更新日期:1990-04-01 00:00:00

  • Daily Maximum Erythemal Dose Rates in the Tropics.

    abstract::The maximum erythemal dose rate (EDRmax ) at the Earth's surface tends to occur at local noon. However, clouds can make the timing of EDRmax significantly away from local noon. In fact, EDRmax and its time of occurrence depend mainly on the solar zenith angle at noon (SZAn ), site's altitude, the total ozone column (T...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13054

    authors: Silva AA,Yamamoto ALC,Corrêa MP

    更新日期:2019-05-01 00:00:00

  • Ab initio quantum chemical investigation of the first steps of the photocycle of phototropin: a model study.

    abstract::Phototropin is a blue light-activated photoreceptor that plays a dominant role in the phototropism of plants. The protein contains two subunits that bind flavin mononucleotide (FMN), which are responsible for the initial steps of the light-induced reaction. It has been proposed that the photoexcited flavin molecule ad...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)077<0101:aiqcio>2.0.co;2

    authors: Neiss C,Saalfrank P

    更新日期:2003-01-01 00:00:00

  • How do UV photomorphogenic responses confer water stress tolerance?

    abstract::Although ultraviolet-B (UV-B) radiation is potentially harmful, it is an important component of terrestrial radiation to which plants have been exposed since invading land. Since then, plants have evolved mechanisms to avoid and repair UV radiation damage; therefore, it is not surprising that photomorphogenic response...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1562/0031-8655(2003)078<0529:hduprc>2.0.co;2

    authors: Gitz DC,Liu-Gitz L

    更新日期:2003-12-01 00:00:00

  • Magnetic resonance imaging evaluation of photodynamic therapy-induced hemorrhagic necrosis in the murine M1 tumor model.

    abstract::Proton magnetic resonance imaging (MRI) and histological methods were used to evaluate photodynamic therapy (PDT)-induced hemorrhagic necrosis in the murine M1 tumor within 72 h of treatment of male DBA/2 mice. The effects of three photosensitizing drugs were investigated: Photofrin (n = 4), Zn (II) phthalocyanine (n ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1997.tb03236.x

    authors: Winsborrow BG,Grondey H,Savoie H,Fyfe CA,Dolphin D

    更新日期:1997-12-01 00:00:00

  • Light-induced starch degradation in non-dormant turions of Spirodela polyrhiza.

    abstract::Continuous red light controls starch degradation in turions of Spirodela polyrhiza [Dölger, K., U. K. Tirlapur and K.-J. Appenroth [1997] Photochem. Photobiol. 66, 126-127 (1997)]. This light could be replaced by repeated red light pulses with the reciprocity law fulfilled over a large range of fluence rates. The effe...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)073<0077:lisdin>2.0.co;2

    authors: Appenroth KJ,Gabrys H

    更新日期:2001-01-01 00:00:00

  • Construction and characterization of hybrid luciferases coded by lux genes from Xenorhabdus luminescens and Vibrio fischeri.

    abstract::Molecular cloning techniques were employed to obtain hybrid luciferases with their alpha and beta subunits encoded by luxA and luxB genes, respectively, from Xenorhabdus luminescens strain HW or Vibrio fischeri. Although the two wild-type luminous bacteria are phylogenetically diverged, the hybrid luciferase Xf compri...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1993.tb02943.x

    authors: Xi L,Tu SC

    更新日期:1993-04-01 00:00:00