Abstract:
:The Escherichia coli protein YcgF contains a photosensory flavin adenine dinucleotide (FAD)-binding BLUF domain covalently linked to an EAL domain, which is predicted to have cyclic-di-guanosine monophosphate (GMP) phosphodiesterase activity. We have cloned, overexpressed and purified this protein, which we refer to as blue light-regulated phosphodiesterase (Blrp) for its putative activity. Blrp undergoes a reversible photocycle after exposure to light in which the spectrum of its photostationary state and kinetics of recovery of the dark state are similar to those of the isolated BLUF domain of the AppA protein. Unlike the AppA BLUF domain, the chromophore environment in the context of full-length Blrp is asymmetric, and the protein does not undergo any detectable global changes on exposure to blue light. When overexpressed in E. coli, Blrp copurifies with certain proteins which suggests that it plays a protective role in response to oxidative stress. Predicted proteins from Klebsiella pneumoniae and from a bacterium in the Sargasso Sea are similar to E. coli Blrp in both their BLUF and EAL domains, which suggests that blue light sensing in these bacteria may follow similar pathways.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Rajagopal S,Key JM,Purcell EB,Boerema DJ,Moffat Kdoi
10.1562/2004-06-16-RA-203subject
Has Abstractpub_date
2004-11-01 00:00:00pages
542-7issue
3eissn
0031-8655issn
1751-1097pii
2004-06-16-RA-203journal_volume
80pub_type
杂志文章abstract::Betanin (betanidin 5-O-β-D-glucoside) is a water-soluble plant pigment used as a color additive in food, drugs and cosmetic products. Despite its sensitivity to light and heat, betanin maintains appreciable tinctorial strength in low acidic and neutral conditions, where the color of other plant pigments, such as antho...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12897
更新日期:2018-09-01 00:00:00
abstract::Ring-fused retinal analogs were designed to examine the hula-twist mode of the photoisomerization of the 9-cis retinylidene chromophore. Two 9-cis retinal analogs, the C11-C13 five-membered ring-fused and the C12-C14 five-membered ring-fused retinal derivatives, formed the pigments with opsin. The C11-C13 ring-fused a...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)076<0606:coosot>2.0.co;2
更新日期:2002-12-01 00:00:00
abstract::The efficacy of photodynamic therapy (PDT) depends upon the amount of photosensitizer accumulated in the malignant tissues. Radachlorin is a popular photosensitizer used in photodynamic therapy to treat various types of cancer. In this study, we have studied the main organelles responsible for the accumulation of rada...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12273
更新日期:2014-09-01 00:00:00
abstract::Alignment of the primary structures of the hydrozoan photoproteins, aequorin, mitrocomin, clytin and obelin showed very strong amino acid sequence identities. The Ca(2+)-binding sites of the proteins were found to be highly conserved. The Ca(2+)-binding sites were also homologous to the Ca(2+)-binding sites of other C...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb08713.x
更新日期:1995-10-01 00:00:00
abstract::Cyclooxygenase (COX)-2 inhibitors are known to be used as chemopreventative agents against certain malignancies. Thus far, there has been very limited information on whether COX-2 inhibitors protect against chronic narrow-band UVB (NB-UVB)-induced immunosuppression. The present study investigated the effect of nonsele...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2007.00289.x
更新日期:2008-03-01 00:00:00
abstract::Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expres...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01232.x
更新日期:2013-03-01 00:00:00
abstract::As a new class of sequence-specific regulators of gene expression, the microRNAs (miRNA) form a regulatory network with growth factors and transcription factors participating in various biological processes. It is now being recognized that the various key processes involved in cancer induction are under the control of...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12346
更新日期:2015-01-01 00:00:00
abstract::This paper reviews briefly the evidence for an association between various measures of UV radiation (UVR) exposure and the development of cancer. Issues such as data quality, study design, measurement variation, comparability of studies, and quantification of UV exposure in relation to skin cancer are discussed. A ran...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1562/2005-02-18-IR-445
更新日期:2005-11-01 00:00:00
abstract::We have demonstrated that it is possible to launch UVC LED light into bacterial contaminated polymer tubes/catheters and disinfect the intraluminal space of these tubes. This can be achieved by UVC treatment of the catheters on a regular basis. Catheters are in the distal end equipped with an exit hole for administrat...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2011.00887.x
更新日期:2011-03-01 00:00:00
abstract::Endogenous cellular chromophores absorb ultraviolet A radiation (UVA, 290-320 nm), the major UV component of terrestrial solar radiation, leading to the formation of reactive oxidizing species that initiate apoptosis, gene expression and mutagenesis. UVA-induced apoptosis of T helper cells is believed to underlie the ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)078<0061:uarira>2.0.co;2
更新日期:2003-07-01 00:00:00
abstract::Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb01736.x
更新日期:1990-04-01 00:00:00
abstract::Porous ZnS and ZnO nano-crystal films were fabricated via a three-step procedure. First, Zn(CH3 COO)2 /Silk Fibroin nanofiber mats were prepared by coaxial electrospinning. Second, Zn(CH3 COO)2 /Silk Fibroin mats were immersed in NaS solution to react with S2- to obtain ZnS/Silk Fibroin nanofiber mats. Finally, ZnO po...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12836
更新日期:2018-01-01 00:00:00
abstract::This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00570.x
更新日期:2009-09-01 00:00:00
abstract::A new type of photochromic molecule that can be used for diffusion coefficient (D) measurements of various proteins in solution is described. The absorption spectrum of this molecule is changed upon photoexcitation by the trans-cis isomerization reaction. Target proteins were labeled by this photochromic molecule in t...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00315.x
更新日期:2008-07-01 00:00:00
abstract::The neonatal immune environment and the events that occur during this time have profound effects for the adult period. While protective immune responses can develop, the neonatal immune system, particularly the skin immune system (SIS), tends to promote tolerance. With this information we undertook a number of studies...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/j.1751-1097.2007.00246.x
更新日期:2008-01-01 00:00:00
abstract::Bacteriochlorophyll (BChl) e was coassembled with BChl c in Triton X-100 micelles in aqueous solutions. The Qy absorption bands of the coaggregates were positioned between those of aggregates consisting solely of BChl c or e. The electronic absorption spectra of the coaggregates could not be reproduced by linear combi...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12219
更新日期:2014-05-01 00:00:00
abstract::In clinical 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT) of skin tumors it is desirable to develop vehicles that minimize the penetration of ALA through normal stratum corneum and maximize it through the compromised stratum corneum of the tumors to improve tumor selectivity. We have designed a bioadhes...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-08-08-RA-641
更新日期:2006-05-01 00:00:00
abstract::This article presents a facile method to prepare CdS/SiO(2) composite microspheres and their good catalytic properties. In our method, monodispersed SiO(2) particles bearing amino groups (-NH(2)) were synthesized at first and then used as carriers to load nanosized CdS particles to form CdS/SiO(2) composite microspher...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01188.x
更新日期:2012-11-01 00:00:00
abstract::Cypridina luciferase (Cluc), a secreted luminescent protein identified from Cypridina noctiluca, has two N-glycosylation sites. In this study, we evaluated the effects of N-glycosylation on Cluc properties by creating site-directed mutagenic modifications at the consensus sequence for N-glycosylation (Asn-X-Ser/Thr). ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12847
更新日期:2018-03-01 00:00:00
abstract::In current clinical practice, photodynamic therapy (PDT) is carried out with prescribed drug doses and light doses as well as fixed drug-light intervals and illumination fluence rates. This approach can result in undesirable treatment outcomes of either overtreatment or undertreatment because of biological variations ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0398:mpfapb>2.0.co;2
更新日期:2002-04-01 00:00:00
abstract::The chloride effect on the photobleaching process of iodopsin, a chicken red-sensitive cone visual pigment, was studied in detail by time-resolved low-temperature spectroscopy at -40 degrees C to -10 degrees C. Decay-associated difference spectra obtained by kinetic analysis using the singular value decomposition meth...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00332.x
更新日期:2008-07-01 00:00:00
abstract::This research examines the behavior of ground-level solar UV radiation as measured by eight broadband meters in the continental United States during the period from late 1994 to late 1998. The goal is to define the variability that occurs in UV irradiance over time scales ranging from one to several years. The monthly...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)072<0488:aaibos>2.0.co;2
更新日期:2000-10-01 00:00:00
abstract::The process of photoinduced hydroxylaminolysis has been re-examined in different bacteriorhodopsin (BR)-based media using O-substituted hydroxylamines, in particular, O-(4-nitrobenzyl) hydroxylamine hydrochloride (NBHA), O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride (FBHA) and O-(t-butyl) hydroxylamine h...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00817.x
更新日期:2010-11-01 00:00:00
abstract::Cationic polythiophenes have been shown to be potent antimicrobial compounds due to their ability to absorb visible light and sensitize the production of reactive oxygen species (ROS) as well as their ability to selectively associate with and damage negatively charged cell envelopes. This study demonstrates the abilit...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13013
更新日期:2018-11-01 00:00:00
abstract::Photodynamic treatment is often thought to produce reactive oxygen species (ROS) that directly induce killing; the nomenclature and phrases revolve around such notions of light-dependency. Few studies reference the possible existence of oxidation products formed in secondary reactions, which bear cytotoxicity competit...
journal_title:Photochemistry and photobiology
pub_type: 评论,杂志文章
doi:10.1111/php.12984
更新日期:2019-01-01 00:00:00
abstract::The photodynamic effect of hypericin on EMT6 mouse mammary carcinoma cells was investigated in vitro under aerobic and hypoxic conditions. Under aerobic conditions, hypericin-induced photocytotoxicity was dose dependent within a 1-50 microM range. Under hypoxic conditions, cells were resistant to hypericin-induced pho...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1992.tb08531.x
更新日期:1992-06-01 00:00:00
abstract::Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD-MA)-PDT of MAT-LyLu cells in vitro. Cells were incubated with BPD-MA concentrations of 0.1, 0.5 or 2.5 μg mL(-1) for 2 h and t...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2011.00942.x
更新日期:2011-09-01 00:00:00
abstract::Albino hairless mice (Skh: HR-1) exposed chronically to sub-erythemal doses of UV radiation display physical, visible and histological alterations. Using narrow bandwidth radiation covering the UV radiation spectrum from 280-380 nm, the wavelength dependence of these alterations was determined. The wavelength dependen...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb02907.x
更新日期:1989-12-01 00:00:00
abstract::We investigated the hypothesis that the strength of the activation of the intra-S DNA damage checkpoint varies within the S phase. Synchronized diploid human fibroblasts were exposed to either 0 or 2.5 J m(-2) UVC in early, mid- and late-S phase. The endpoints measured were the following: (1) radio-resistant DNA synth...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12361
更新日期:2015-01-01 00:00:00
abstract::Herbal extracts including asiatic acid (AA) have become popular candidates of anti-photoaging agents due to their anti-inflammatory and antioxidant properties and minimal side effect. Nevertheless, low bioavailability due to poor solubility limits their practical application. In this study, a highly bioavailable form ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13160
更新日期:2020-01-01 00:00:00