Purification and initial characterization of a putative blue light-regulated phosphodiesterase from Escherichia coli.


:The Escherichia coli protein YcgF contains a photosensory flavin adenine dinucleotide (FAD)-binding BLUF domain covalently linked to an EAL domain, which is predicted to have cyclic-di-guanosine monophosphate (GMP) phosphodiesterase activity. We have cloned, overexpressed and purified this protein, which we refer to as blue light-regulated phosphodiesterase (Blrp) for its putative activity. Blrp undergoes a reversible photocycle after exposure to light in which the spectrum of its photostationary state and kinetics of recovery of the dark state are similar to those of the isolated BLUF domain of the AppA protein. Unlike the AppA BLUF domain, the chromophore environment in the context of full-length Blrp is asymmetric, and the protein does not undergo any detectable global changes on exposure to blue light. When overexpressed in E. coli, Blrp copurifies with certain proteins which suggests that it plays a protective role in response to oxidative stress. Predicted proteins from Klebsiella pneumoniae and from a bacterium in the Sargasso Sea are similar to E. coli Blrp in both their BLUF and EAL domains, which suggests that blue light sensing in these bacteria may follow similar pathways.


Photochem Photobiol


Rajagopal S,Key JM,Purcell EB,Boerema DJ,Moffat K




Has Abstract


2004-11-01 00:00:00














  • Enhancement of palladium-porphyrin room temperature phosphorescence by alkaline earth metal in deoxycholate aggregates solution.

    abstract::Room temperature phosphorescence (RTP) of three palladium (Pd)-phorphyrins in air-saturated solution of sodium deoxycholate (NaDC) aggregates was measured. RTP of Pd-meso-tetrakis (4-carboxyphenyl) porphyrin (Pd-TCPP) was obviously enhanced in NaDC-aggregates mediated by alkaline earth metal (AEM). Under the same expe...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Wang YT,Wang XW,Zhang Y

    更新日期:2011-07-01 00:00:00

  • Photocatalytic degradation of toluene using a novel flow reactor with Fe-doped TiO2 catalyst on porous nickel sheets.

    abstract::A novel continuous-flow photocatalytic reactor was designed to decompose toluene by using porous nickel sheets that were coated with a Fe-doped TiO2 catalyst. While locating the UV lamp at the central axis, the catalyst sheets were located along the inner wall and positioned vertically with an equal space of 50 mm alo...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Dezhi S,Sheng C,Chung JS,Xiaodong D,Zhibin Z

    更新日期:2005-03-01 00:00:00

  • Low-level laser therapy ameliorates CCl4-induced liver cirrhosis in rats.

    abstract::This study investigated the effects of low-level laser therapy (LLLT) in the liver function, structure and inflammation in a experimental model of carbon tetrachloride (CCl(4))-induced liver cirrhosis. Wistar rats were divided into Control, LLLT, CCl(4) and CCl(4) +LLLT groups. CCl(4) groups received CCl(4) (0.4 g kg(...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Oliveira-Junior MC,Monteiro AS,Leal-Junior EC,Munin E,Osório RA,Ribeiro W,Vieira RP

    更新日期:2013-01-01 00:00:00

  • Do years or quanta age the retina?

    abstract::The progressive loss of visual faculties with advancing age is attributed, sometimes, mainly to allegedly inevitable optical deterioration, such as clouding of the media, or again to neurosenescence, as typified by cell-death. The relevant factors are assessed, and it is not thought likely that changes in normal ocula...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审


    authors: Weale RA

    更新日期:1989-09-01 00:00:00

  • Quenching of singlet molecular oxygen (1O2) by azide anion in solvent mixtures.

    abstract::The azide ion is a strong physical quencher of singlet molecular oxygen (1O2) and is frequently employed to show involvement of 1O2 in oxidation processes. Rate constants (k(q)) for the quenching of 1O2 by azide are routinely used as standards to calculate k(q) values for quenching by other substrates. We have measure...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Li MY,Cline CS,Koker EB,Carmichael HH,Chignell CF,Bilski P

    更新日期:2001-12-01 00:00:00

  • Water and carboxyl group environments in the dehydration blueshift of bacteriorhodopsin.

    abstract::The proton channels of the bacteriorhodopsin (BR) proton pump contain bound water molecules. The channels connect the purple membrane surfaces with the protonated retinal Schiff base at the membrane center. Films of purple membrane equilibrated at low relative humidity display a shift of the 570 nm retinal absorbance ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Renthal R,Gracia N,Regalado R

    更新日期:2000-11-01 00:00:00

  • Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis.

    abstract::Urinary cytology is a noninvasive and unconstraining technique for urothelial cancer diagnosis but lacks sensitivity for detecting low-grade lesions. In this study, the fluorescence properties of classical Papanicolaou-stained urothelial cytological slides from patients or from cell lines were monitored to investigate...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Steenkeste K,Lécart S,Deniset A,Pernot P,Eschwège P,Ferlicot S,Lévêque-Fort S,Briandet R,Fontaine-Aupart MP

    更新日期:2007-09-01 00:00:00

  • Photoactivated coumaryl-diazopyruvate fluorescent label for amine functional groups of tissues containing type-I collagen.

    abstract::The design, synthesis and application of a new fluorescent-labeling reagent for collagen has been developed as a prerequisite for the design of a photoactivated collagen-crosslinking compound for surgical wound closure. The amine groups in collagen are the targets of a rational design for a new fluorophore because nat...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Timberlake GT,Reddy GK,Stehno-Bittel L,Weber JF,Amslinger S,Givens RS

    更新日期:2002-11-01 00:00:00

  • Transient and stationary spectroscopy of cytochrome c: ultrafast internal conversion controls photoreduction.

    abstract::Photoreduction of cytochrome c (Cyt c) has been reinvestigated using femtosecond-to-nanosecond transient absorption and stationary spectroscopy. Femtosecond spectra of oxidized Cyt c, recorded in the probe range 270-1000 nm, demonstrate similar evolution upon 266 or 403 nm excitation: an ultrafast 0.3 ps internal conv...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Löwenich D,Kleinermanns K,Karunakaran V,Kovalenko SA

    更新日期:2008-01-01 00:00:00

  • Photodynamic inactivation of the dermatophyte Trichophyton rubrum.

    abstract::Dermatophytes are fungi that can cause infections (known as tinea) of the skin, hair and nails because of their ability to use keratin. Superficial mycoses are probably the most prevalent of infectious diseases worldwide. One of the most distinct limitations of the current therapeutic options is the recurrence of the ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Smijs TG,Schuitmaker HJ

    更新日期:2003-05-01 00:00:00

  • In vivo spectrophotometric evaluation of neoplastic and non-neoplastic skin pigmented lesions--I. Reflectance measurements.

    abstract::Reflectance spectrophotometry from 400 to 800 nm on different cutaneous pigmented lesions, including primary and metastatic malignant melanoma, pigmented nevi, lentigo and seborrhoeic keratosis, has been performed by using an external integrating sphere coupled to a spectrophotometer. Measurements show that reflectanc...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Marchesini R,Brambilla M,Clemente C,Maniezzo M,Sichirollo AE,Testori A,Venturoli DR,Cascinelli N

    更新日期:1991-01-01 00:00:00

  • Fluorescence quantum yields and their relation to lifetimes of rhodamine 6G and fluorescein in nine solvents: improved absolute standards for quantum yields.

    abstract::Absolute fluorescence quantum yields are reported for the rhodamine 6G cation and the fluorescein dianion dyes in nine solvents. This information is combined with previously reported fluorescence lifetimes to deduce radiative and nonradiative decay rates. Along the alcohol series from methanol to octanol, rhodamine 6G...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Magde D,Wong R,Seybold PG

    更新日期:2002-04-01 00:00:00

  • Role of xanthurenic acid 8-O-beta-D-glucoside, a novel fluorophore that accumulates in the brunescent human eye lens.

    abstract::We have been able to identify a blue fluorophore from the low-molecular weight soluble fraction of human adult nondiabetic brunescent cataract lenses as xanthurenic acid 8-O-beta-D-glucoside (XA8OG) (excitation = 338 nm and emission = 440 nm). To determine the role of this fluorophore in the lens, we have examined its...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Thiagarajan G,Shirao E,Ando K,Inoue A,Balasubramanian D

    更新日期:2002-09-01 00:00:00

  • In vivo near-infrared Raman spectroscopy: demonstration of feasibility during clinical gastrointestinal endoscopy.

    abstract::Raman spectroscopy (RS) has potential for disease classification within the gastrointestinal tract (GI). A near-infrared (NIR) fiber-optic RS system has been developed previously. This study reports the first in vivo Raman spectra of human gastrointestinal tissues measured during routine clinical endoscopy. This was a...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Shim MG,Song LM,Marcon NE,Wilson BC

    更新日期:2000-07-01 00:00:00

  • A Photoprotective Effect by Cation-π-Interaction? Quenching of Singlet Oxygen by an Indole Cation-π Model System.

    abstract::We investigated the effect of the cation-π interaction on the susceptibility of a tryptophan model system toward interaction with singlet oxygen, that is, type II photooxidation. The model system consists of two indole units linked to a lariat crown ether to measure the total rate of removal of singlet oxygen by the i...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Arevalo GE,Cagan DA,Monsour CG,Garcia AC,McCurdy A,Selke M

    更新日期:2020-11-01 00:00:00

  • Action spectra of phthalocyanines with respect to photosensitization of cells.

    abstract::Human carcinoma cells (NHIK 3025 cells) and Chinese hamster cells (V79 cells) were incubated with AlPcS1, AlPcS2 and AlPcS4, phthalocyanines with different lipophilicity but with similar photochemical properties when in monomeric solutions. The absorption- and fluorescence spectra of the dyes in the cells were recorde...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Moan J,Berg K,Bommer JC,Western A

    更新日期:1992-08-01 00:00:00

  • Feasibility of using fluoresceinyl Cypridina luciferin analog in a novel chemiluminescence method for real-time photodynamic therapy dosimetry.

    abstract::Singlet oxygen ((1)O(2)) is the most important cytotoxic agent in photodynamic therapy (PDT). The feasibility of using a chemiluminescence (CL) probe, 3,7-dihydro-6-[4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl]-2-methylimidazo{1,2-a}pyrazin-3-one sodium salt (fluoresceinyl Cypridina luciferin analog, FCLA), to ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Qin Y,Xing D,Luo S,Zhou J,Zhong X,Chen Q

    更新日期:2005-11-01 00:00:00

  • Effects of demethylchlortetracycline phototoxicity on the structure and functions of rat liver mitochondria.

    abstract::The phototoxic effects of demethylchlortetracycline (DMCT) with UVA radiation on isolated rat liver mitochondria were studied. DMCT at concentration of 21.5 microM with 5.53 x 10(-2) J cm-3 of UVA was found to be a potent uncoupler of oxidative phosphorylation. DMCT alone also uncoupled mitochondria but at higher conc...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Takagi S,Mizuno N,Adachi K,Wakabayashi T

    更新日期:1990-02-01 00:00:00

  • Damage to DNA in bacterioplankton: a model of damage by ultraviolet radiation and its repair as influenced by vertical mixing.

    abstract::A model of UV-induced DNA damage in oceanic bacterioplankton was developed and tested against previously published and novel measurements of cyclobutane pyrimidine dimers (CPD) in surface layers of the ocean. The model describes the effects of solar irradiance, wind-forced mixing of bacterioplankton and optical proper...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Huot Y,Jeffrey WH,Davis RF,Cullen JJ

    更新日期:2000-07-01 00:00:00

  • Physicochemical studies of demetalation of light-harvesting bacteriochlorophyll isomers purified from green sulfur photosynthetic bacteria.

    abstract::Demetalation kinetics of bacteriochlorophylls (BChls) c, d and e from green sulfur photosynthetic bacteria were studied under weakly acidic conditions. Demetalation rate constants of BChl e possessing a formyl group at the 7-position were significantly smaller than those of BChls c and d, which had a methyl group at t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Hirai Y,Tamiaki H,Kashimura S,Saga Y

    更新日期:2009-09-01 00:00:00

  • Use of potassium iodide as a chemical actinometer.

    abstract::Aqueous solutions of KI were examined for use as chemical actinometers to measure 254 nm (germicidal) radiation. Irradiation results in electron ejection from iodide such that aqueous electrons and iodine atoms are formed. In the presence of N2O, an electron scavenger, recombination of these two reactive species is el...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Rahn RO

    更新日期:1993-12-01 00:00:00

  • Effects of UVR and UVR-induced cytokines on production of extracellular matrix proteins and proteases by dermal fibroblasts cultured in collagen gels%.

    abstract::Synthesis of extracellular matrix (ECM) proteins and their degradation by matrix metalloproteinases (MMP) are part of the dermal remodeling resulting from chronic exposure of skin to ultraviolet radiation (UVR). We have compared two alternative mechanisms for these responses, namely, a direct mechanism in which UV-B o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Kossodo S,Wong WR,Simon G,Kochevar IE

    更新日期:2004-01-01 00:00:00

  • Photooxidation products of merocyanine 540 formed under preactivation conditions for tumor therapy.

    abstract::In order to gain insight into the preactivation of merocyanine 540 (MC540) 1 for the photodynamic therapy (Gulliya et al., 1990a, Photochem. Photobiol. 52, 831-838) its photo-oxidation was investigated. After irradiation of MC540 1 on a preparative scale three main photodegradation products were isolated with 16-20% y...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Franck B,Schneider U

    更新日期:1992-08-01 00:00:00

  • Analysis of phosphorescence decay in heterogeneous systems: consequences of finite excitation flash duration.

    abstract::Analysis of phosphorescence lifetimes using the Stern-Volmer equation is a reliable means of determining quencher concentration for a uniform sample. Methods of analysis for heterogeneous systems are based on the assumption that the excitation is produced by a momentary flash. This condition is an idealization because...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Golub AS,Popel AS,Zheng L,Pittman RN

    更新日期:1999-06-01 00:00:00

  • Enhanced UV-B radiation increases glyphosate resistance in velvetleaf (Abutilon theophrasti).

    abstract::Depletion of the ozone layer leads to increasing UV-B radiation on the earth's surface, which may affect weeds and their responses to herbicides. However, the effect of increased UV-B radiation on weeds and the interaction of weeds and herbicides are still obscure. The objective of this study was to compare glyphosate...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Yin L,Zhang M,Li Z,Duan L,Wang S

    更新日期:2012-11-01 00:00:00

  • Psoralen Derivatives with Enhanced Potency.

    abstract::Psoralen is a furocoumarin natural product that intercalates within DNA and forms covalent adducts when activated by ultraviolet radiation. It is well known that this property contributes to psoralen's clinical efficacy in several disease contexts, which include vitiligo, psoriasis, graft-versus-host disease and cutan...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Buhimschi AD,Gooden DM,Jing H,Fels DR,Hansen KS,Beyer WF Jr,Dewhirst MW,Walder H,Gasparro FP

    更新日期:2020-09-01 00:00:00

  • Immunotoxic effects of cis-urocanic acid exposure in C57BL/6N and C3H/HeN mice.

    abstract::Exposure to ultraviolet radiation results in increased levels of intradermal cis-urocanic acid (cUCA) and alters cutaneous immunity by interfering with processing and presentation of antigen by Langerhans cells. Reports on effects of systemic immunotoxicity with 30 day cUCA exposure in laboratory rodents include thymi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Prater MR,Gogal RM Jr,De Fabo EC,Longstreth J,Holladay SD

    更新日期:2003-04-01 00:00:00

  • Protective properties of novel S-acyl-glutathione thioesters against ultraviolet-induced oxidative stress.

    abstract::UV-induced toxicity is characterized by marked oxidative stress, accompanied by the depletion of key cellular antioxidants, particularly glutathione (GSH). Replenishing cellular GSH may represent a means of counteracting UV-induced toxicity: however, treatment with free GSH is not therapeutically effective due to its ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Wright D,Zampagni M,Evangelisti E,Conti S,D'Adamio G,Goti A,Becatti M,Fiorillo C,Taddei N,Cecchi C,Liguri G

    更新日期:2013-03-01 00:00:00

  • Chemical Analysis of the Luminous Slime Secreted by the Marine Worm Chaetopterus (Annelida, Polychaeta).

    abstract::The marine annelid Chaetopterus variopedatus produces bioluminescence by an unknown and potentially novel mechanism. We have advanced the study of this fascinating phenomenon, which has not been investigated for nearly 60 years after initial studies were first reported for this species. Here, we show that the luminous...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Branchini BR,Behney CE,Southworth TL,Rawat R,Deheyn DD

    更新日期:2014-01-01 00:00:00

  • The hairless mouse model of photoaging: evaluation of the relationship between dermal elastin, collagen, skin thickness and wrinkles.

    abstract::Quantitative and qualitative changes in dermal collagen and elastin occur in response to chronic ultraviolet (UV) irradiation. These changes have been implicated in the genesis of the wrinkling seen in chronically irradiated, or photoaged skin. We examined the relationship between wrinkle formation and changes in derm...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章


    authors: Moloney SJ,Edmonds SH,Giddens LD,Learn DB

    更新日期:1992-10-01 00:00:00