Abstract：
:Photodynamic therapy (PDT) oxygen consumption, clonogenic cell survival, fluorescence photobleaching and photoproduct formation were investigated during benzoporphyrin derivative monoacid (BPD-MA)-PDT of MAT-LyLu cells in vitro. Cells were incubated with BPD-MA concentrations of 0.1, 0.5 or 2.5 μg mL(-1) for 2 h and then treated with 405 nm light under oxygenated and hypoxic conditions. Fluorescence spectra were acquired during treatment, and photobleaching and photoproduct generation were quantified using singular value decomposition of the spectra. Cell survival was measured at set times during the treatment using a colony-forming assay. The amount of oxygen consumed by PDT per photon absorbed decreased with BPD-MA intracellular concentration. Survival was correlated with the total amount of oxygen consumed by PDT per unit volume, which is assumed to be equivalent to the amount of singlet oxygen that reacted. A photobleaching-based singlet oxygen dose metric was also found to predict survival independent of intracellular BPD-MA concentration. The BPD-MA photoproduct was bleached during the treatment. Two singlet oxygen dose metrics based on photoproduct kinetics could not be correlated with cell survival over the full range of intracellular BPD-MA concentrations used.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Weston MA,Patterson MSdoi
10.1111/j.1751-1097.2011.00942.xsubject
Has Abstractpub_date
2011-09-01 00:00:00pages
1129-37issue
5eissn
0031-8655issn
1751-1097journal_volume
87pub_type
杂志文章abstract：:The thermodynamics of binding of Me-alpha(-GalNAc, Gal-beta-1-3GalNAc-alpha-O-Me (T-antigen-alpha), Gal-beta-1-3GalNAc and Gal-alpha-1-6Glc (mellibiose) to Artocarpus hirsuta lectin was studied using fluorescence spectroscopy. The binding affinities of the saccharides are in the order Gal-beta-1-3GalNAc-alpha-O-Me > M...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2006-05-04-RN-892
更新日期：2006-09-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01975.x
更新日期：1990-05-01 00:00:00
abstract：:This work evaluated the photoprotective and antigenotoxic effects against ultraviolet B (UVB) radiation of flavonoid compounds apigenin, naringenin and pinocembrin. The photoprotective efficacy of these compounds was estimated using in vitro photoprotection indices, and the antigenotoxicity against UVB radiation was e...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13085
更新日期：2019-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00699.x
更新日期：2010-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0493:SCMIBU>2.0.CO;2
更新日期：2001-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12157
更新日期：2014-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0398:mpfapb>2.0.co;2
更新日期：2002-04-01 00:00:00
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更新日期：2010-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0318:uaicfm>2.0.co;2
更新日期：2001-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1991.tb02011.x
更新日期：1991-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb05673.x
更新日期：1996-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00880.x
更新日期：2011-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05139.x
更新日期：1994-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb02194.x
更新日期：1992-10-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13245
更新日期：2020-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb02409.x
更新日期：1995-12-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
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更新日期：2002-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05052.x
更新日期：1994-03-01 00:00:00
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doi：
更新日期：1999-01-01 00:00:00
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journal_title：Photochemistry and photobiology
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更新日期：2008-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
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更新日期：2019-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.1996.tb02450.x
更新日期：1996-08-01 00:00:00
abstract：:The quantum yield, phi ZE, for configurational photoisomerization (4Z,15Z----4Z,15E) of bilirubin bound non-covalently to human serum albumin was determined (at 23 +/- 2 degrees C) by laser excitation and chromatographic analysis of products. Values obtained for photoexcitation at 465 nm were about one-half those prev...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb04226.x
更新日期：1992-02-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb08510.x
更新日期：1992-05-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1989.tb08436.x
更新日期：1989-05-01 00:00:00
abstract：:DNA UVB dosimeters, consisting of minidots of dried bacteriophage DNA placed on a UV-transparent polymer film, were analyzed by polymerase chain reactions (PCR). Ultraviolet-B dosimetry obtained with amplification of phage lambda DNA of segments of 1.08 kilobase pairs (kbp) and 2.24 kbp was compared with that obtained...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1997.tb03205.x
更新日期：1997-11-01 00:00:00
abstract：:We have used the complex of antigen with class II major histocompatibility proteins (Ia) in membrane-bound form to target a phototoxic compound to antigen-specific T cell hybridomas in vitro. The iodoacetamidyl ester of phototoxic pyrene was bound covalently to antigen-presenting cells (APC), and protein antigens were...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb02352.x
更新日期：1995-05-01 00:00:00
abstract：:Cultured cells accumulate acridine orange (AO), which is a weak basic dye and a photosensitizer, in lysosomes and other acidic compartments. During exposure to blue light, AO-loaded macrophages show decreasing red granular fluorescence and increasing green diffuse fluorescence. This is hypothesized to represent peroxi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01685.x
更新日期：1990-01-01 00:00:00
