Isolation and characterization of a subunit form of the B875 light-harvesting complex from Rhodobacter capsulatus.

Abstract:

:A structural subunit (called B816) has been isolated from the B875 light-harvesting complex of Rhodobacter capsulatus using a detergent-mediated dissociation of chromatophores. Rb. capsulatus MW442 (B800-850- B875+ car+) chromatophores were extracted with benzene and titrated with octyl glucoside (OG) to shift the near-infrared absorption maximum from 873 to 816 nm. Gel filtration chromatography was then used to separate B816 from reaction centers. B816 could be quantitatively shifted back to a B875-like form (lambda max = 875 nm) by decreasing the OG concentration. A similar B816 species could be isolated in low yield from wildtype (B800-850+ B875+ car+) cells but not from SB203E (B800-850- B875+ car-). In the latter case, the B816 subunit seemed too unstable to be isolated under equivalent conditions. The alpha:beta polypeptide ratio, the CD spectrum, and the ability to reversibly dissociate B816 to free bacteriochlorophyll and alpha- and beta-polypeptides were found to be similar to those of the B820 subunit of Rs. rubrum previously reported by our laboratory.

journal_name

Photochem Photobiol

authors

Heller BA,Loach PA

doi

10.1111/j.1751-1097.1990.tb01975.x

subject

Has Abstract

pub_date

1990-05-01 00:00:00

pages

621-7

issue

5

eissn

0031-8655

issn

1751-1097

journal_volume

51

pub_type

杂志文章
  • Spectroscopic studies on self-aggregation of bacteriochlorophyll-e in nonpolar organic solvents: effects of stereoisomeric configuration at the 3(1)-position and alkyl substituents at the 8(1)-position.

    abstract::Self-aggregation of naturally occurring bacteriochlorophyll (BChl)-e in nonpolar organic solvents was investigated by visible absorption, fluorescence emission and circular dichroism spectra. Cultured brown-colored photosynthetic bacteria have several BChl-e as light-harvesting antenna pigments. Three major BChl-e hom...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)074<0072:ssosao>2.0.co;2

    authors: Saga Y,Matsuura K,Tamiaki H

    更新日期:2001-07-01 00:00:00

  • Comparison of photosensitizer (AIPcS2) quantification techniques: in situ fluorescence microsampling versus tissue chemical extraction.

    abstract::A noninvasive in situ fluorescence-based method for the quantification of the photosensitizer chloroaluminum disulfonated phthalocyanine was compared to the highly accurate but nonreal time ex vivo spectrofluorometry method. Our in vivo fluorescence technique is designed to allow real-time assessment of photosensitize...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)074<0453:copaqt>2.0.co;2

    authors: Lee CC,Pouge BW,Strawbridge RR,Moodie KL,Bartholomew LR,Burke GC,Hoopes PJ

    更新日期:2001-09-01 00:00:00

  • A comparative study of the visible light photochemistry of gilvocarcins V and M.

    abstract::Quantum yields for the formation of superoxide ions, O2-., and singlet oxygen, 1O2, were determined during the photolyses of gilvocarcin M (GM) in air-saturated dry dimethylsulfoxide (DMSO) and in 45:55 (vol/vol) DMSO-water mixtures. The quantum yield for the photoreduction of methyl viologen by GM in nitrogen-saturat...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1995.tb02361.x

    authors: Alegría AE,Zayas L,Guevara N

    更新日期:1995-09-01 00:00:00

  • Monitoring photoproduct formation and photobleaching by fluorescence spectroscopy has the potential to improve PDT dosimetry with a verteporfin-like photosensitizer.

    abstract::In current clinical practice, photodynamic therapy (PDT) is carried out with prescribed drug doses and light doses as well as fixed drug-light intervals and illumination fluence rates. This approach can result in undesirable treatment outcomes of either overtreatment or undertreatment because of biological variations ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2002)075<0398:mpfapb>2.0.co;2

    authors: Zeng H,Korbelik M,McLean DI,MacAulay C,Lui H

    更新日期:2002-04-01 00:00:00

  • Infrared radiation influence on molt and regeneration of Neohelice granulata Dana, 1851 (Grapsidae, Sesarminae).

    abstract::This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00570.x

    authors: Gonzalez VC,Lena AB,Espindola WA,de Souza Votto AP,Jorge MB,de Moraes Vaz Batista Filgueira D,Bianchini A,Sérgio Varela Júnior A,Nery LE,Trindade GS

    更新日期:2009-09-01 00:00:00

  • Photosensitized oxidation and inactivation of pyocyanin, a virulence factor of Pseudomonas aeruginosa.

    abstract::Pyocyanin (PyO-) (1-hydroxy-5-methylphenazine) is a cytotoxic compound secreted by Pseudomonas aeruginosa, an omnipresent bacterium and a human pathogen. We report that visible light illumination in the presence of rose bengal, or riboflavin, in aerated solutions (pH 7.0-7.2) induces irreversible loss of the pigment's...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-07-29-RA-626

    authors: Reszka KJ,Denning GM,Britigan BE

    更新日期:2006-03-01 00:00:00

  • Solvothermal Synthesis of Cs0.33 WO3 /LDHs Composite as a Novel Visible-Light-Driven Photocatalyst.

    abstract::A novel Cs0.33 WO3 /LDHs (CWLDH) composite was synthesized by simple two steps solvothermal method and first investigated as the photocatalyst for tetracycline (TC) and Congo red (CR) degradation under visible light irradiation. The CWLDH heterostructures catalysts were characterized by XRD, UV-Vis, SEM, XPS and BET. ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12838

    authors: Zhang D,Ji T,Yu J,Jiang X,Jiao F

    更新日期:2018-03-01 00:00:00

  • Damage to DNA in bacterioplankton: a model of damage by ultraviolet radiation and its repair as influenced by vertical mixing.

    abstract::A model of UV-induced DNA damage in oceanic bacterioplankton was developed and tested against previously published and novel measurements of cyclobutane pyrimidine dimers (CPD) in surface layers of the ocean. The model describes the effects of solar irradiance, wind-forced mixing of bacterioplankton and optical proper...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)072<0062:dtdiba>2.0.co;2

    authors: Huot Y,Jeffrey WH,Davis RF,Cullen JJ

    更新日期:2000-07-01 00:00:00

  • Radical cation generation from singlet and triplet excited states of all-trans-lycopene in chloroform.

    abstract::On direct photoexcitation, subpicosecond time-resolved absorption spectroscopy revealed that the 1B(u)-type singlet excited state of all-trans-lycopene in chloroform was about seven times more efficient than all-trans-beta-carotene in generating the radical cation. The time constant of radical cation generation from t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2004-04-22-RA-147

    authors: Han RM,Wu YS,Feng J,Ai XC,Zhang JP,Skibsted LH

    更新日期:2004-09-01 00:00:00

  • Ultraviolet light induces double-strand breaks in DNA of cultured human P3 cells as measured by neutral filter elution.

    abstract::Neutral filter elution at pH 7.2 and 9.6 was used to measure the induction of DNA lesions in human P3 teratocarcinoma cells by monochromatic 254-, 270-, 313-, 334-, 365-, and 405-nm radiation and by 60 gamma rays. In this assay DNA double-strand breaks (dsb) increase the rate of elution of DNA from cell lysates on a f...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1990.tb04194.x

    authors: Peak JG,Peak MJ

    更新日期:1990-08-01 00:00:00

  • Measurement of ultraviolet exposure in epidemiological studies of skin and skin cancers.

    abstract::As our understanding of the role of UV in causing skin cancer continues to expand, researchers and clinicians must continue to remain up to date on the various means by which UV exposure can be quantified. The purpose of this article was to review the current methods used to measure lifetime exposure to UV and to summ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00367.x

    authors: Worswick SD,Cockburn M,Peng D

    更新日期:2008-11-01 00:00:00

  • Simplified categorization of outdoor activities for male and female U.S. indoor workers-a feasibility study to improve assessment of ultraviolet radiation exposures in epidemiologic study questionnaires.

    abstract::Skin cancer studies depend on questionnaires to estimate exposure to ultraviolet light and subsequent risk but are limited by recall bias. We investigate the feasibility of developing a short checklist of categories comprising outdoor activities that can improve sun exposure questionnaires for use in epidemiologic stu...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00393.x

    authors: Kwok RK,Linet MS,Chodick G,Kleinerman RA,Freedman DM,Fears T,Johnson RE,Alexander BH

    更新日期:2009-01-01 00:00:00

  • Daily duration of vitamin D synthesis in human skin with relation to latitude, total ozone, altitude, ground cover, aerosols and cloud thickness.

    abstract::Vitamin D production in human skin occurs only when incident UV radiation exceeds a certain threshold. From simulations of UV irradiances worldwide and throughout the year, we have studied the dependency of the extent and duration of cutaneous vitamin D production in terms of latitude, time, total ozone, clouds, aeros...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2004-11-19-RN-375

    authors: Engelsen O,Brustad M,Aksnes L,Lund E

    更新日期:2005-11-01 00:00:00

  • Construction and characterization of hybrid luciferases coded by lux genes from Xenorhabdus luminescens and Vibrio fischeri.

    abstract::Molecular cloning techniques were employed to obtain hybrid luciferases with their alpha and beta subunits encoded by luxA and luxB genes, respectively, from Xenorhabdus luminescens strain HW or Vibrio fischeri. Although the two wild-type luminous bacteria are phylogenetically diverged, the hybrid luciferase Xf compri...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1993.tb02943.x

    authors: Xi L,Tu SC

    更新日期:1993-04-01 00:00:00

  • Supramolecular cationic tetraruthenated porphyrin and light-induced decomposition of 2'-deoxyguanosine predominantly via a singlet oxygen-mediated mechanism.

    abstract::The tetraruthenated porphyrin, mu-[meso-5,10,15,20-tetra(pyridyl)porphyrin]tetrakis[bis-(bipyridine) chloride ruthenium(II)] (TRP) is a supramolecular cationic species. The aim of the present investigation was to evaluate the photodynamic properties of TRP and Zn-TRP to damage DNA with emphasis on the mechanistic aspe...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Ravanat JL,Cadet J,Araki K,Toma HE,Medeiros MH,Mascio PD

    更新日期:1998-11-01 00:00:00

  • Second Rhagophthalmid Luciferase Cloned from Chinese Glow-worm Menghuoius giganteus (Rhagophthalmidae: Elateroidea).

    abstract::The pH-insensitive beetle luciferases cloned from Rhagophthalmidae, Phengodidae, and Elateridae exhibit great potential application as reporter assays for monitoring gene expression. At present, however, only one luciferase has been reported from the enigmatic and predominantly Asian distributed luminous family Rhagop...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13172

    authors: Liu GC,Dong ZW,Hou QB,He JW,Zhao RP,Wang W,Li XY

    更新日期:2020-01-01 00:00:00

  • Promotion of photodynamic therapy-induced apoptosis by the mitochondrial protein Smac/DIABLO: dependence on Bax.

    abstract::Photodynamic therapy (PDT) using the second-generation photosensitizer phthalocyanine (Pc) 4 causes mitochondrial damage and induces apoptosis through the release of cytochrome c to the cytosol. Another protein of the mitochondrial intermembrane space, Smac/DIABLO (second mitochondria-derived activator of caspase/dire...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2002)076<0217:poptia>2.0.co;2

    authors: Usuda J,Chiu SM,Azizuddin K,Xue LY,Lam M,Nieminen AL,Oleinick NL

    更新日期:2002-08-01 00:00:00

  • Interaction between N-terminal loop and beta-scaffold of photoactive yellow protein.

    abstract::During the photoreaction cycle of photoactive yellow protein (PYP), a physiologically active intermediate (PYP(M)) is formed as a consequence of global protein conformational change. Previous studies have demonstrated that the photocycle of PYP is regulated by the N-terminal loop region, which is located across the ce...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00375.x

    authors: Harigai M,Kataoka M,Imamoto Y

    更新日期:2008-07-01 00:00:00

  • Cyclobutane dimers and (6-4) photoproducts in human cells are mended with the same patch sizes.

    abstract::The size of excision repair patches corresponding to excision of (6-4) pyrimidine-pyrimidone photoproducts and (5-5, 6-6) cyclobutane dimers have been independently determined by using bromodeoxyuridine substitution and density increases in isopycnic gradients of small DNA fragments. The two classes of photoproducts w...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1991.tb02033.x

    authors: Cleaver JE,Jen J,Charles WC,Mitchell DL

    更新日期:1991-09-01 00:00:00

  • Additional evidence for the cyclic GMP signaling pathway resulting in the photophobic behavior of Stentor coeruleus.

    abstract::We report that exo- and endogenous guanosine 3',5'-cyclic monophosphate (cGMP) specifically influenced the photophobic response. In behavioral experiments the slowly hydrolyzable and membrane-permeable analogs of cGMP (8-bromo-cGMP [Br-cGMP] and N6,2'-o-dibutyryl-cGMP) dramatically prolonged the time for ciliary stop ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2001)074<0829:aeftcg>2.0.co;2

    authors: Walerczyk M,Fabczak S

    更新日期:2001-12-01 00:00:00

  • Using computational fluid dynamics modeling to evaluate the design of hospital ultraviolet germicidal irradiation systems for inactivating airborne mycobacteria.

    abstract::This research was conducted to evaluate the design of hospital ultraviolet germicidal irradiation (UVGI) systems and to assess their effectiveness for inactivating airborne mycobacteria. A computational fluid dynamics (CFD) model was developed and tested by simulating previous experiments measuring the effectiveness o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12062

    authors: Xu P,Fisher N,Miller SL

    更新日期:2013-07-01 00:00:00

  • Photophysics and Rotational Dynamics of a Hydrophilic Molecule in a Room Temperature Ionic Liquid.

    abstract::We have studied the photophysics and rotational diffusion of hydrophilic solute 7-(N, N'-diethylamino)coumarin-3-carboxylic acid (7-DCCA) in a room temperature ionic liquid methyltrioctylammonium bis(trifluoromethylsulfonyl) imide ([N1888 ][NTf2 ]). Comparison of activation energies of viscous flow and nonradiative de...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12472

    authors: Chatterjee A,Maity B,Ahmed SA,Seth D

    更新日期:2015-09-01 00:00:00

  • Validation and application of a model of oxygen consumption and diffusion during photodynamic therapy in vitro.

    abstract::The photophysical parameters for the photosensitizer Pd(II) meso-Tetra(4-carboxyphenyl) porphine (PdT790) acquired in a previous study were incorporated into the PDT oxygen diffusion models for cell suspensions and cell monolayers. The time-dependent phosphorescence signals generated by the diffusion models are shown ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12320

    authors: Weston MA,Patterson MS

    更新日期:2014-11-01 00:00:00

  • Photoreduction of bacteriorhodopsin Schiff base at low humidity. A study with C13=C14 nonisomerizable artificial pigments.

    abstract::The retinal protonated Schiff base of bacteriorhodopsin is photoreactive to reducing agents such as NaBH4. In the present work we have studied the effect of different protein hydration levels on the photoreductive reaction, as well as the consequences of preventing isomerization around the critical C13=C14 retinal dou...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2002)075<0668:pobsba>2.0.co;2

    authors: Aharoni A,Ottolenghi M,Sheves M

    更新日期:2002-06-01 00:00:00

  • A role for hydrogen peroxide in the pro-apoptotic effects of photodynamic therapy.

    abstract::Although the first reactive oxygen species (ROS) formed during irradiation of photosensitized cells is almost invariably singlet molecular oxygen (1O2), other ROS have been implicated in the phototoxic effects of photodynamic therapy (PDT). Among these are superoxide anion radical (*O2(-)), hydrogen peroxide (H2O2) an...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00589.x

    authors: Price M,Terlecky SR,Kessel D

    更新日期:2009-11-01 00:00:00

  • Solar UVB dosimetry by amplification of short and long segments in phage lambda DNA.

    abstract::DNA UVB dosimeters, consisting of minidots of dried bacteriophage DNA placed on a UV-transparent polymer film, were analyzed by polymerase chain reactions (PCR). Ultraviolet-B dosimetry obtained with amplification of phage lambda DNA of segments of 1.08 kilobase pairs (kbp) and 2.24 kbp was compared with that obtained...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1997.tb03205.x

    authors: Yoshida H,Regan JD

    更新日期:1997-11-01 00:00:00

  • Protective properties of novel S-acyl-glutathione thioesters against ultraviolet-induced oxidative stress.

    abstract::UV-induced toxicity is characterized by marked oxidative stress, accompanied by the depletion of key cellular antioxidants, particularly glutathione (GSH). Replenishing cellular GSH may represent a means of counteracting UV-induced toxicity: however, treatment with free GSH is not therapeutically effective due to its ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2012.01231.x

    authors: Wright D,Zampagni M,Evangelisti E,Conti S,D'Adamio G,Goti A,Becatti M,Fiorillo C,Taddei N,Cecchi C,Liguri G

    更新日期:2013-03-01 00:00:00

  • How Does Photoreceptor UVR8 Perceive a UV-B Signal?

    abstract::UVR8 is the only known plant photoreceptor that mediates light responses to UV-B (280-315 nm) of the solar spectrum. UVR8 perceives a UV-B signal via light-induced dimer dissociation, which triggers a wide range of cellular responses involved in photomorphogenesis and photoprotection. Two recent crystal structures of ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12470

    authors: Yang X,Montano S,Ren Z

    更新日期:2015-09-01 00:00:00

  • Estimating the Diurnal Cycle and Daily Insolation of Ultraviolet and Photosynthetically Active Radiation at the Sea Surface.

    abstract::Accurate determination of the diurnal variability and daily insolation of surface (0(+) ) and subsurface (0(-) ) irradiance are essential to estimate several physical, chemical and biological processes occurring at the surface layer of marine environments. Natural downwelling PAR and spectral UVR were examined on eigh...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12474

    authors: Kuwahara VS,Taguchi S

    更新日期:2015-09-01 00:00:00

  • Solar UV exposure of seafarers along subtropical and tropical shipping routes.

    abstract::Seafarers working on decks of vessels at low latitudes are exposed to extremely high solar UV radiation. Their risk of developing skin cancer may be enhanced. Solar erythemal UV irradiance and exposure were measured for the first time on merchant vessels going along typical international routes at low latitudes. The m...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12144

    authors: Feister U,Meyer G,Kirst U

    更新日期:2013-11-01 00:00:00