Abstract:
:A structural subunit (called B816) has been isolated from the B875 light-harvesting complex of Rhodobacter capsulatus using a detergent-mediated dissociation of chromatophores. Rb. capsulatus MW442 (B800-850- B875+ car+) chromatophores were extracted with benzene and titrated with octyl glucoside (OG) to shift the near-infrared absorption maximum from 873 to 816 nm. Gel filtration chromatography was then used to separate B816 from reaction centers. B816 could be quantitatively shifted back to a B875-like form (lambda max = 875 nm) by decreasing the OG concentration. A similar B816 species could be isolated in low yield from wildtype (B800-850+ B875+ car+) cells but not from SB203E (B800-850- B875+ car-). In the latter case, the B816 subunit seemed too unstable to be isolated under equivalent conditions. The alpha:beta polypeptide ratio, the CD spectrum, and the ability to reversibly dissociate B816 to free bacteriochlorophyll and alpha- and beta-polypeptides were found to be similar to those of the B820 subunit of Rs. rubrum previously reported by our laboratory.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Heller BA,Loach PAdoi
10.1111/j.1751-1097.1990.tb01975.xsubject
Has Abstractpub_date
1990-05-01 00:00:00pages
621-7issue
5eissn
0031-8655issn
1751-1097journal_volume
51pub_type
杂志文章abstract::Self-aggregation of naturally occurring bacteriochlorophyll (BChl)-e in nonpolar organic solvents was investigated by visible absorption, fluorescence emission and circular dichroism spectra. Cultured brown-colored photosynthetic bacteria have several BChl-e as light-harvesting antenna pigments. Three major BChl-e hom...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)074<0072:ssosao>2.0.co;2
更新日期:2001-07-01 00:00:00
abstract::A noninvasive in situ fluorescence-based method for the quantification of the photosensitizer chloroaluminum disulfonated phthalocyanine was compared to the highly accurate but nonreal time ex vivo spectrofluorometry method. Our in vivo fluorescence technique is designed to allow real-time assessment of photosensitize...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)074<0453:copaqt>2.0.co;2
更新日期:2001-09-01 00:00:00
abstract::Quantum yields for the formation of superoxide ions, O2-., and singlet oxygen, 1O2, were determined during the photolyses of gilvocarcin M (GM) in air-saturated dry dimethylsulfoxide (DMSO) and in 45:55 (vol/vol) DMSO-water mixtures. The quantum yield for the photoreduction of methyl viologen by GM in nitrogen-saturat...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb02361.x
更新日期:1995-09-01 00:00:00
abstract::In current clinical practice, photodynamic therapy (PDT) is carried out with prescribed drug doses and light doses as well as fixed drug-light intervals and illumination fluence rates. This approach can result in undesirable treatment outcomes of either overtreatment or undertreatment because of biological variations ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0398:mpfapb>2.0.co;2
更新日期:2002-04-01 00:00:00
abstract::This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00570.x
更新日期:2009-09-01 00:00:00
abstract::Pyocyanin (PyO-) (1-hydroxy-5-methylphenazine) is a cytotoxic compound secreted by Pseudomonas aeruginosa, an omnipresent bacterium and a human pathogen. We report that visible light illumination in the presence of rose bengal, or riboflavin, in aerated solutions (pH 7.0-7.2) induces irreversible loss of the pigment's...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-07-29-RA-626
更新日期:2006-03-01 00:00:00
abstract::A novel Cs0.33 WO3 /LDHs (CWLDH) composite was synthesized by simple two steps solvothermal method and first investigated as the photocatalyst for tetracycline (TC) and Congo red (CR) degradation under visible light irradiation. The CWLDH heterostructures catalysts were characterized by XRD, UV-Vis, SEM, XPS and BET. ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12838
更新日期:2018-03-01 00:00:00
abstract::A model of UV-induced DNA damage in oceanic bacterioplankton was developed and tested against previously published and novel measurements of cyclobutane pyrimidine dimers (CPD) in surface layers of the ocean. The model describes the effects of solar irradiance, wind-forced mixing of bacterioplankton and optical proper...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)072<0062:dtdiba>2.0.co;2
更新日期:2000-07-01 00:00:00
abstract::On direct photoexcitation, subpicosecond time-resolved absorption spectroscopy revealed that the 1B(u)-type singlet excited state of all-trans-lycopene in chloroform was about seven times more efficient than all-trans-beta-carotene in generating the radical cation. The time constant of radical cation generation from t...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-04-22-RA-147
更新日期:2004-09-01 00:00:00
abstract::Neutral filter elution at pH 7.2 and 9.6 was used to measure the induction of DNA lesions in human P3 teratocarcinoma cells by monochromatic 254-, 270-, 313-, 334-, 365-, and 405-nm radiation and by 60 gamma rays. In this assay DNA double-strand breaks (dsb) increase the rate of elution of DNA from cell lysates on a f...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb04194.x
更新日期:1990-08-01 00:00:00
abstract::As our understanding of the role of UV in causing skin cancer continues to expand, researchers and clinicians must continue to remain up to date on the various means by which UV exposure can be quantified. The purpose of this article was to review the current methods used to measure lifetime exposure to UV and to summ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00367.x
更新日期:2008-11-01 00:00:00
abstract::Skin cancer studies depend on questionnaires to estimate exposure to ultraviolet light and subsequent risk but are limited by recall bias. We investigate the feasibility of developing a short checklist of categories comprising outdoor activities that can improve sun exposure questionnaires for use in epidemiologic stu...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00393.x
更新日期:2009-01-01 00:00:00
abstract::Vitamin D production in human skin occurs only when incident UV radiation exceeds a certain threshold. From simulations of UV irradiances worldwide and throughout the year, we have studied the dependency of the extent and duration of cutaneous vitamin D production in terms of latitude, time, total ozone, clouds, aeros...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-11-19-RN-375
更新日期:2005-11-01 00:00:00
abstract::Molecular cloning techniques were employed to obtain hybrid luciferases with their alpha and beta subunits encoded by luxA and luxB genes, respectively, from Xenorhabdus luminescens strain HW or Vibrio fischeri. Although the two wild-type luminous bacteria are phylogenetically diverged, the hybrid luciferase Xf compri...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb02943.x
更新日期:1993-04-01 00:00:00
abstract::The tetraruthenated porphyrin, mu-[meso-5,10,15,20-tetra(pyridyl)porphyrin]tetrakis[bis-(bipyridine) chloride ruthenium(II)] (TRP) is a supramolecular cationic species. The aim of the present investigation was to evaluate the photodynamic properties of TRP and Zn-TRP to damage DNA with emphasis on the mechanistic aspe...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::The pH-insensitive beetle luciferases cloned from Rhagophthalmidae, Phengodidae, and Elateridae exhibit great potential application as reporter assays for monitoring gene expression. At present, however, only one luciferase has been reported from the enigmatic and predominantly Asian distributed luminous family Rhagop...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13172
更新日期:2020-01-01 00:00:00
abstract::Photodynamic therapy (PDT) using the second-generation photosensitizer phthalocyanine (Pc) 4 causes mitochondrial damage and induces apoptosis through the release of cytochrome c to the cytosol. Another protein of the mitochondrial intermembrane space, Smac/DIABLO (second mitochondria-derived activator of caspase/dire...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)076<0217:poptia>2.0.co;2
更新日期:2002-08-01 00:00:00
abstract::During the photoreaction cycle of photoactive yellow protein (PYP), a physiologically active intermediate (PYP(M)) is formed as a consequence of global protein conformational change. Previous studies have demonstrated that the photocycle of PYP is regulated by the N-terminal loop region, which is located across the ce...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00375.x
更新日期:2008-07-01 00:00:00
abstract::The size of excision repair patches corresponding to excision of (6-4) pyrimidine-pyrimidone photoproducts and (5-5, 6-6) cyclobutane dimers have been independently determined by using bromodeoxyuridine substitution and density increases in isopycnic gradients of small DNA fragments. The two classes of photoproducts w...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1991.tb02033.x
更新日期:1991-09-01 00:00:00
abstract::We report that exo- and endogenous guanosine 3',5'-cyclic monophosphate (cGMP) specifically influenced the photophobic response. In behavioral experiments the slowly hydrolyzable and membrane-permeable analogs of cGMP (8-bromo-cGMP [Br-cGMP] and N6,2'-o-dibutyryl-cGMP) dramatically prolonged the time for ciliary stop ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2001)074<0829:aeftcg>2.0.co;2
更新日期:2001-12-01 00:00:00
abstract::This research was conducted to evaluate the design of hospital ultraviolet germicidal irradiation (UVGI) systems and to assess their effectiveness for inactivating airborne mycobacteria. A computational fluid dynamics (CFD) model was developed and tested by simulating previous experiments measuring the effectiveness o...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12062
更新日期:2013-07-01 00:00:00
abstract::We have studied the photophysics and rotational diffusion of hydrophilic solute 7-(N, N'-diethylamino)coumarin-3-carboxylic acid (7-DCCA) in a room temperature ionic liquid methyltrioctylammonium bis(trifluoromethylsulfonyl) imide ([N1888 ][NTf2 ]). Comparison of activation energies of viscous flow and nonradiative de...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12472
更新日期:2015-09-01 00:00:00
abstract::The photophysical parameters for the photosensitizer Pd(II) meso-Tetra(4-carboxyphenyl) porphine (PdT790) acquired in a previous study were incorporated into the PDT oxygen diffusion models for cell suspensions and cell monolayers. The time-dependent phosphorescence signals generated by the diffusion models are shown ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12320
更新日期:2014-11-01 00:00:00
abstract::The retinal protonated Schiff base of bacteriorhodopsin is photoreactive to reducing agents such as NaBH4. In the present work we have studied the effect of different protein hydration levels on the photoreductive reaction, as well as the consequences of preventing isomerization around the critical C13=C14 retinal dou...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0668:pobsba>2.0.co;2
更新日期:2002-06-01 00:00:00
abstract::Although the first reactive oxygen species (ROS) formed during irradiation of photosensitized cells is almost invariably singlet molecular oxygen (1O2), other ROS have been implicated in the phototoxic effects of photodynamic therapy (PDT). Among these are superoxide anion radical (*O2(-)), hydrogen peroxide (H2O2) an...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00589.x
更新日期:2009-11-01 00:00:00
abstract::DNA UVB dosimeters, consisting of minidots of dried bacteriophage DNA placed on a UV-transparent polymer film, were analyzed by polymerase chain reactions (PCR). Ultraviolet-B dosimetry obtained with amplification of phage lambda DNA of segments of 1.08 kilobase pairs (kbp) and 2.24 kbp was compared with that obtained...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1997.tb03205.x
更新日期:1997-11-01 00:00:00
abstract::UV-induced toxicity is characterized by marked oxidative stress, accompanied by the depletion of key cellular antioxidants, particularly glutathione (GSH). Replenishing cellular GSH may represent a means of counteracting UV-induced toxicity: however, treatment with free GSH is not therapeutically effective due to its ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01231.x
更新日期:2013-03-01 00:00:00
abstract::UVR8 is the only known plant photoreceptor that mediates light responses to UV-B (280-315 nm) of the solar spectrum. UVR8 perceives a UV-B signal via light-induced dimer dissociation, which triggers a wide range of cellular responses involved in photomorphogenesis and photoprotection. Two recent crystal structures of ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12470
更新日期:2015-09-01 00:00:00
abstract::Accurate determination of the diurnal variability and daily insolation of surface (0(+) ) and subsurface (0(-) ) irradiance are essential to estimate several physical, chemical and biological processes occurring at the surface layer of marine environments. Natural downwelling PAR and spectral UVR were examined on eigh...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12474
更新日期:2015-09-01 00:00:00
abstract::Seafarers working on decks of vessels at low latitudes are exposed to extremely high solar UV radiation. Their risk of developing skin cancer may be enhanced. Solar erythemal UV irradiance and exposure were measured for the first time on merchant vessels going along typical international routes at low latitudes. The m...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12144
更新日期:2013-11-01 00:00:00