当前位置： SCI文献检索 > PHOTOCHEMISTRY AND PHOTOBIOLOGY期刊下所有文献 > Why is rose bengal more phototoxic to fibroblasts in vitro than in vivo?

Why is rose bengal more phototoxic to fibroblasts in vitro than in vivo?

Abstract：

:Photosensitized protein cross-linking has been recently developed to seal wounds and strengthen tissue. Although the photosensitizing dye, Rose Bengal (RB), is phototoxic to cultured cells, cytotoxicity does not accompany RB-photosensitized tissue repair in vivo. We investigated whether the environment surrounding cells in tissue or the high irradiances used for photo-cross-linking inhibited RB phototoxicity. Fibroblasts (FB) grown within collagen gels to mimic a tissue environment and monolayer cultured FB were treated with RB (0.01-1 mm) and the high 532 nm laser irradiances used in vivo for tissue repair (0.10-0.50 W cm(-2)). Monolayer FB were substantially more sensitive to RB photosensitization: the LD50 was >200-fold lower than that in collagen gels. Collagen gel protection was associated with increased Akt phosphorylation, a prosurvival pathway. RB phototoxicity in collagen gels was 25-fold greater at low (0.030 W cm(-2)) that at high (0.50 W cm(-2)) irradiances. Oxygen depletion at high irradiance only partially accounted for the irradiance dependence of phototoxicity as replacing air with nitrogen only increased the LD50 by four-fold in monolayers. These results indicate that the lack of RB phototoxicity during in vivo tissue repair results from upregulation of prosurvival pathways in tissue cells, oxygen depletion and irradiance-dependent RB photochemistry.

journal_name

Photochem Photobiol

journal_title

Photochemistry and photobiology

authors

Yao M,Gu C,Doyle FJ Jr,Zhu H,Redmond RW,Kochevar IE

doi

10.1111/php.12215

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

297-305

issue

2

eissn

0031-8655

issn

1751-1097

journal_volume

90

pub_type

杂志文章

相关文献

PHOTOCHEMISTRY AND PHOTOBIOLOGY文献大全
  • Simplified calibration for broadband solar ultraviolet radiation measurements.

    abstract：:Aspects of different calibration procedures for erythemally weighing broadband radiometers are presented in this study. These instruments are common in projects dealing with ultraviolet radiation effects on humans. Many erythemally weighing broadband radiometers are still operated using a single calibration factor (cf...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2003)078<0603:SCFBSU>2.0.CO;2

    authors： Oppenrieder A,Hoeppe P,Koepke P,Reuder J,Schween J,Schreder J

    更新日期：2003-12-01 00:00:00

  • In vitro Studies of Antitumor Effect, Toxicity/Cytotoxicity and Skin Permeation/Retention of a Green Fluorescence Pyrene-based Dye for PDT Application.

    abstract：:Photosensitizers (PS) are compounds that can generate reactive oxygen species under irradiation of appropriate light and are widely used in photodynamic therapy (PDT). Currently, topical PDT is an effective treatment for several skin diseases, including bacterial infections, fungal mycoses and psoriasis. In addition, ...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.13335

    authors： de França BM,Ghasemishahrestani Z,de Souza GFM,da Silva RN,Queiroz DD,Pierre MBR,Pereira MD,Forero JSB,Corrêa RJ

    更新日期：2020-09-23 00:00:00

  • Parabolic quantitative structure-activity relationships and photodynamic therapy: application of a three-compartment model with clearance to the in vivo quantitative structure-activity relationships of a congeneric series of pyropheophorbide derivatives u

    abstract：:An open three-compartment pharmacokinetic model was applied to the in vivo quantitative structure-activity relationship (QSAR) data of a homologous series of pyropheophorbide photosensitizers for photodynamic therapy (PDT). The physical model was a lipid compartment sandwiched between two identical aqueous compartment...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：

    authors： Potter WR,Henderson BW,Bellnier DA,Pandey RK,Vaughan LA,Weishaupt KR,Dougherty TJ

    更新日期：1999-11-01 00:00:00

  • New 5-aminolevulinic acid esters--efficient protoporphyrin precursors for photodetection and photodynamic therapy.

    abstract：:Photodetection (PD) and photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PPIX) accumulation are approaches to detect and treat dysplasia and early cancer in the gastrointestinal tract and in the urinary bladder. Because ALA-induced PPIX production is limited, we synthesized ALA es...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2003)078<0481:naaepp>2.0.co;2

    authors： Brunner H,Hausmann F,Knuechel R

    更新日期：2003-11-01 00:00:00

  • Behaviors producing photodispersal in Stentor coeruleus.

    abstract：:The ciliate Stentor coeruleus exhibits photodispersal, that is, these cells swim away from light sources and collect in dimly lighted areas. We imaged and reconstructed the tracks of 48 Stentor to determine which swimming behaviors produced their photodispersal. We observed that their photodispersal is not due to a ch...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2004)080<0401:BPPISC>2.0.CO;2

    authors： Menzies E,Das N,Wood DC

    更新日期：2004-11-01 00:00:00

  • Ultraviolet light induces double-strand breaks in DNA of cultured human P3 cells as measured by neutral filter elution.

    abstract：:Neutral filter elution at pH 7.2 and 9.6 was used to measure the induction of DNA lesions in human P3 teratocarcinoma cells by monochromatic 254-, 270-, 313-, 334-, 365-, and 405-nm radiation and by 60 gamma rays. In this assay DNA double-strand breaks (dsb) increase the rate of elution of DNA from cell lysates on a f...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1990.tb04194.x

    authors： Peak JG,Peak MJ

    更新日期：1990-08-01 00:00:00

  • Effect of netropsin on one-electron oxidation of duplex DNA.

    abstract：:The effect of netropsin on the oxidative reactions of duplex DNA was examined. One-electron oxidation of DNA creates a radical cation that migrates through duplex DNA and reacts primarily at GG steps. Netropsin is a dication that specifically binds primarily by hydrogen bonding in the minor groove at sites that have f...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2004)080<0456:EONOOO>2.0.CO;2

    authors： Roberts LW,Schuster GB

    更新日期：2004-11-01 00:00:00

  • UVB radiation affects growth, reproduction and tissue structure of Daphnia magna across several temperatures.

    abstract：:We examined the effects of daily exposure to UVB on growth, reproduction and histological characteristics of Daphnia magna over two generations at 20, 22, 25 and 30°C. Animals were exposed to 16 h of UVA and photosynthetically active radiation daily. Treated animals received 6 h of UVB during the light phase. Parental...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2012.01197.x

    authors： Huebner JD,Loadman NL,Wiegand MD,Huebner E,Palitsky DJ,Husarewycz WH

    更新日期：2013-01-01 00:00:00

  • Analysis of two kinds of tree as physical barriers against erythemal UVB radiation received.

    abstract：:Differences between global radiation UVER (erythemal ultraviolet solar radiation) received under full sun and diffuse radiation received under the shadow of two types of tree are analyzed to check the importance of these components on human exposure to UV radiation. Blue Line spores dosimeters of VioSpor were used for...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12020

    authors： Ysasi GG,Ribera LJ

    更新日期：2013-05-01 00:00:00

  • Effects of low-power laser irradiation on cell locomotion in protozoa.

    abstract：:Low-power lasers are commonly used in human medicine for treatment of various pathological conditions, but mechanisms of their healing effects are still poorly understood. The results of this study provide information related to these effects at the cellular level. Two different protozoan species, Euglena gracilis and...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2004)080<0531:EOLLIO>2.0.CO;2

    authors： Koutna M,Janisch R,Unucka M,Svobodnik A,Mornstein V

    更新日期：2004-11-01 00:00:00

  • Formation of protein-bound 3,4-dihydroxyphenylalanine in collagen types I and IV exposed to ultraviolet light.

    abstract：:Collagen was exposed to an ultraviolet (UV) lamp that emitted predominantly in the UVB range. The cross-linking of collagen type I and type IV by UV irradiation was observed. Amino acid analyses revealed that Tyr residues in both collagen types I and IV were decreased by irradiation. In collagen type IV, losses of His...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1995.tb08624.x

    authors： Kato Y,Nishikawa T,Kawakishi S

    更新日期：1995-04-01 00:00:00

  • DNA damage and repair in Gorlin syndrome and normal fibroblasts after aminolevulinic acid photodynamic therapy: a comet assay study.

    abstract：:Using normal, untransformed, human fibroblasts, the effectiveness of aminolevulinic (ALA)-mediated photodynamic therapy (PDT) was investigated in terms of both clonogenic survival and DNA damage. The response of normal fibroblasts was then compared with Gorlin syndrome-derived fibroblasts (basal cell nevus syndrome [B...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2003)078<0337:ddarig>2.0.co;2

    authors： Haylett AK,Ward TH,Moore JV

    更新日期：2003-10-01 00:00:00

  • Photosensitization by Photofrin II delivered to WI26VA4 SV40-transformed human fibroblasts by low density lipoproteins: inhibition of lipid synthesis and fatty acid uptake.

    abstract：:Irradiation with 365 nm light of Wi26VA4 SV40-transformed human fibroblasts cultured for 24 h in the presence of low density lipoproteins loaded with the anticancer porphyrin mixture Photofrin II resulted in a near complete inhibition of [14C]oleic acid incorporation into triacylglycerols, cholesteryl esters and phosp...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1992.tb04209.x

    authors： Biade S,Mazière JC,Mora L,Santus R,Morlière P,Mazière C,Salmon S,Gatt S,Dubertret L

    更新日期：1992-01-01 00:00:00

  • Comparative analysis of GPCR crystal structures.

    abstract：:The phototransduction cascade is perhaps the best understood model system for G protein-coupled receptor (GPCR) signaling. Phototransduction links the absorption of a single photon of light to a decrease in cytosolic cGMP. Depletion of the cGMP pool induces closure of cGMP-gated cation channels resulting in the hyperp...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1751-1097.2008.00516.x

    authors： Lodowski DT,Angel TE,Palczewski K

    更新日期：2009-03-01 00:00:00

  • Enhancement of fluoresceinyl cypridina luciferin analog chemiluminescence by human serum albumin for singlet oxygen detection.

    abstract：:Fluoresceinyl cypridina luciferin analog (FCLA) is a chemiluminescence (CL) probe for detecting reactive oxygen species (ROS). Its efficiency for detecting singlet oxygen (1O2) can be significantly enhanced in the presence of human serum albumin (HSA). This phenomenon may apply to important applications for both resea...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/2005-12-02-ra-744

    authors： Zhou J,Xing D,Chen Q

    更新日期：2006-07-01 00:00:00

  • X-ray induced visible luminescence of porphyrins.

    abstract：:A concept has been presented whereby X-ray irradiation can be used to induce visible luminescence of chromophores such as porphyrins. The essential aspects of the idea have been demonstrated in a supramolecular assembly consisting of a water-dispersed polystyrene latex with an embedded scintillating compound, 2,5-diph...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1994.tb05052.x

    authors： Kokotov S,Lewis A,Neumann R,Amrusi S

    更新日期：1994-03-01 00:00:00

  • Luciferin-Regenerating Enzyme Mediates Firefly Luciferase Activation Through Direct Effects of D-Cysteine on Luciferase Structure and Activity.

    abstract：:Luciferin-regenerating enzyme (LRE) contributes to in vitro recycling of D-luciferin. In this study, reinvestigation of the luciferase-based LRE assay is reported. Here, using quick change site-directed mutagenesis seven T-LRE (Lampyris turkestanicusLRE) mutants were constructed and the most functional mutant of T-LRE...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12430

    authors： Hemmati R,Hosseinkhani S,Sajedi RH,Azad T,Tashakor A,Bakhtiari N,Ataei F

    更新日期：2015-07-01 00:00:00

  • The Mechanism of CIRP in Regulation of STAT3 Phosphorylation and Bag-1/S Expression Upon UVB Radiation.

    abstract：:Cold-inducible RNA binding protein (CIRP) is a stress-inducible protein, which could be activated by various cellular stresses, such as hypothermia, hypoxia and UV irradiation. Our previous study indicated that UVB radiation (3 mJ cm-2 ) induces CIRP expression, which promotes keratinocytes growth, survival and eventu...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12981

    authors： Sun W,Liao Y,Yi Q,Wu S,Tang L,Tong L

    更新日期：2018-11-01 00:00:00

  • Effects of UVR and UVR-induced cytokines on production of extracellular matrix proteins and proteases by dermal fibroblasts cultured in collagen gels%.

    abstract：:Synthesis of extracellular matrix (ECM) proteins and their degradation by matrix metalloproteinases (MMP) are part of the dermal remodeling resulting from chronic exposure of skin to ultraviolet radiation (UVR). We have compared two alternative mechanisms for these responses, namely, a direct mechanism in which UV-B o...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：

    authors： Kossodo S,Wong WR,Simon G,Kochevar IE

    更新日期：2004-01-01 00:00:00

  • Hypocrellin A photosensitization involves an intracellular pH decrease in 3T3 cells.

    abstract：:The fluorescent pH probe carboxy-seminaphtorhodafluor-1 (C-Snarf-1) has been used for laser microspectro-fluorometric assays of intracellular pH in 3T3 mouse fibroblasts treated with hypocrellin A. These results are compared to those previously obtained with the structurally related hydroxylated polycyclic quinone, hy...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：

    authors： Chaloupka R,Sureau F,Kocisova E,Petrich JW

    更新日期：1998-07-01 00:00:00

  • Sensitized photooxidation of Di- and tripeptides of tyrosine.

    abstract：:This paper studies the dye-sensitized photooxidation of tyrosine (tyr) and tyr di- and tripeptides (tyr-tyr and tyr-tyr-tyr) mediated by singlet molecular oxygen (O2[1 delta g]) in alkaline media. Photooxidation quantum efficiencies (phi r) were obtained by determining the overall and reactive rate constants of intera...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：

    authors： Criado S,Soltermann AT,Marioli JM,García NA

    更新日期：1998-10-01 00:00:00

  • Daily duration of vitamin D synthesis in human skin with relation to latitude, total ozone, altitude, ground cover, aerosols and cloud thickness.

    abstract：:Vitamin D production in human skin occurs only when incident UV radiation exceeds a certain threshold. From simulations of UV irradiances worldwide and throughout the year, we have studied the dependency of the extent and duration of cutaneous vitamin D production in terms of latitude, time, total ozone, clouds, aeros...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/2004-11-19-RN-375

    authors： Engelsen O,Brustad M,Aksnes L,Lund E

    更新日期：2005-11-01 00:00:00

  • Cyanine Modification Tuned for Amine Photorelease.

    abstract：:Cyanines are emerging as useful agents for photoreleasing biological compounds because of their capability of utilizing near-infrared (NIR) light. Another benefit is their ability to self-sensitize to produce singlet oxygen for the release of aryl amines, a process that has not been as feasible in the past. Here, we h...

    journal_title：Photochemistry and photobiology

    pub_type： 评论,杂志文章

    doi：10.1111/php.13102

    authors： Mathew JP,Greer A

    更新日期：2019-09-01 00:00:00

  • Potentiation of oxidative damage to proteins by ultraviolet-A and protection by antioxidants.

    abstract：:We have studied the damage of alcohol dehydrogenase (ADH) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) induced by Fe++/EDTA + H2O2 in combination with UV-A (main output at 365 nm). Enzyme inactivation, formation of hydroxyl radicals (measured in the absence of enzymes), increase in protein carbonyls, oxidation ...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1992.tb02171.x

    authors： Hu ML,Tappel AL

    更新日期：1992-09-01 00:00:00

  • Nanoscale hydroxyl radical generation from multiphoton ionization of tryptophan.

    abstract：:Exposure of solutions containing both tryptophan and hydrogen peroxide to a pulsed ( approximately 180 fs) laser beam at 750 nm induces luminescence characteristic of 5-hydroxytryptophan. The results indicate that 3-photon excitation of tryptophan results in photoionization within the focal volume of the laser beam. T...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2008.00447.x

    authors： Bisby RH,Crisostomo AG,Botchway SW,Parker AW

    更新日期：2009-01-01 00:00:00

  • 6-Nitro-Quinazolin-4(3H)-one Exhibits Photodynamic Effects and Photodegrades Human Melanoma Cell Lines. A Study on the Photoreactivity of Simple Quinazolin-4(3H)-ones.

    abstract：:Photochemo and photodynamic therapies are minimally invasive approaches for the treatment of cancers and powerful weapons for competing bacterial resistance to antibiotics. Synthetic and naturally occurring quinazolinones are considered privileged anticancer and antibacterial agents, with several of them to have emerg...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.13376

    authors： Panagopoulos A,Balalas T,Mitrakas A,Vrazas V,Katsani KR,Koumbis AE,Koukourakis MI,Litinas KE,Fylaktakidou KC

    更新日期：2021-01-01 00:00:00

  • Light-induced starch degradation in non-dormant turions of Spirodela polyrhiza.

    abstract：:Continuous red light controls starch degradation in turions of Spirodela polyrhiza [Dölger, K., U. K. Tirlapur and K.-J. Appenroth [1997] Photochem. Photobiol. 66, 126-127 (1997)]. This light could be replaced by repeated red light pulses with the reciprocity law fulfilled over a large range of fluence rates. The effe...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2001)073<0077:lisdin>2.0.co;2

    authors： Appenroth KJ,Gabrys H

    更新日期：2001-01-01 00:00:00

  • Contact transcleral ciliary body photodynamic therapy with verteporfin in pigmented rabbits: effect of repeated treatments.

    abstract：:We studied the effect on the intraocular pressure (IOP) and the ciliary body (CB) morphology after four consecutive contact transcleral photodynamic treatments of the ciliary body (CB-PDT) with verteporfin in pigmented rabbits. Twenty-two pigmented rabbits underwent CB-PDT (study group), performed once (six rabbits) o...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2009.00638.x

    authors： Charisis SK,Naoumidi II,Ginis HS,Detorakis ET,Tsilimbaris MK

    更新日期：2010-01-01 00:00:00

  • Chlorophyll fluorescence imaging for the noninvasive assessment of anthocyanins in whole grape (Vitis vinifera L.) bunches.

    abstract：:The distribution of anthocyanins in grape (Vitis vinifera L.) bunches from the Sangiovese cultivar was measured nondestructively by chlorophyll fluorescence imaging using two excitation light bands at 550 and 650 nm in sequence. The pixel intensity in the derived logarithm of the fluorescence excitation ratio image wa...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2008.00424.x

    authors： Agati G,Traversi ML,Cerovic ZG

    更新日期：2008-11-01 00:00:00

  • Low-level laser therapy and sodium diclofenac in acute inflammatory response induced by skeletal muscle trauma: effects in muscle morphology and mRNA gene expression of inflammatory markers.

    abstract：:Pharmacological therapy is widely used in the treatment of muscle injuries. On the other hand, low-level laser therapy (LLLT) arises as a promising nonpharmacological treatment. The aim of this study was to analyze the effects of sodium diclofenac (topical application) and LLLT on morphological aspects and gene expres...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2012.01232.x

    authors： de Almeida P,Lopes-Martins RÁ,Tomazoni SS,Albuquerque-Pontes GM,Santos LA,Vanin AA,Frigo L,Vieira RP,Albertini R,de Carvalho Pde T,Leal-Junior EC

    更新日期：2013-03-01 00:00:00