Abstract：
:Cellular autofluorescence spectra were monitored in a single-beam gradient force optical trap ("optical tweezers") in order to probe the physiological effects of near infrared and UVA (320-400 nm) microirradiation. Prior to trapping, Chinese hamster ovary cells exhibited weak UVA-excited autofluorescence with maxima at 455 nm characteristic of beta-nicotinamide adenine dinucleotide (phosphate) emission. No strong effect of a 1064 nm NIR microbeam on fluorescence intensity and spectral characteristics was found during trapping, even for power densities up to 70 MW/cm2 and radiant exposures of 100 GJ/cm2. In contrast to the 1064 nm trap, a 760 nm trapping beam caused a two-fold autofluorescence increase within 5 min (about 20 GJ/cm2). Exposure to 365 nm UVA (1 W/cm2) during 1064 nm trapping significantly altered cellular autofluorescence, causing, within 10 min, a five-fold increase and a 6 nm red shift versus initial levels. We conclude that 1064 nm microbeams can be applied for an extended period without producing autofluorescence changes characteristic of alterations in the cellular redox state. However, 760 nm effects may occur via a two-photon absorption mechanism, which, in a manner similar to UVA exposure, alters the redox balance and places the cell in a state of oxidative stress.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
König K,Liu Y,Sonek GJ,Berns MW,Tromberg BJdoi
10.1111/j.1751-1097.1995.tb09143.xsubject
Has Abstractpub_date
1995-11-01 00:00:00pages
830-5issue
5eissn
0031-8655issn
1751-1097journal_volume
62pub_type
杂志文章abstract：:The exposure of outdoor car guards to solar ultraviolet radiation (UVR), the majority with deeply pigmented skin, to solar UVR was measured for five consecutive days during early spring (September 2017) in South Africa using electronic UVR dosimeters attached to the upper arm of each participant. The exposure of the n...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13008
更新日期：2019-01-01 00:00:00
abstract：:Bacteriochlorophyll (BChl) e was coassembled with BChl c in Triton X-100 micelles in aqueous solutions. The Qy absorption bands of the coaggregates were positioned between those of aggregates consisting solely of BChl c or e. The electronic absorption spectra of the coaggregates could not be reproduced by linear combi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12219
更新日期：2014-05-01 00:00:00
abstract：:Raman spectroscopy (RS) has potential for disease classification within the gastrointestinal tract (GI). A near-infrared (NIR) fiber-optic RS system has been developed previously. This study reports the first in vivo Raman spectra of human gastrointestinal tissues measured during routine clinical endoscopy. This was a...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0146:IVNIRS>2.0.CO;2
更新日期：2000-07-01 00:00:00
abstract：:This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00570.x
更新日期：2009-09-01 00:00:00
abstract：:This review deals with the biophysical aspects of flavin-based photosensors, comprising cryptochromes, LOV (Light, Oxygen and Voltage) and BLUF (Blue Light sensing Using FAD) proteins. Special emphasis is given to structural issues, photocycle quantum yields and energetics, mechanism of the light-triggered reactions, ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.2007.00196.x
更新日期：2007-11-01 00:00:00
abstract：:In the vertebrate retina, rods mediate twilight vision and cones mediate daylight vision. Their photoresponse characteristics are different. The light-sensitivity of a cone is 10(2)-10(3) times lower than that of a rod. In addition, the photoresponse time course is much faster in cones. The mechanism characterizing co...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1562/2006-02-28-IR-823
更新日期：2007-01-01 00:00:00
abstract：:Schinus terebinthifolius is a plant rich in phenolic compounds, which have antioxidant properties and can provide new opportunities for treatment and prevention of diseases mediated by ultraviolet radiation like photoaging and skin cancer. The aim of this study was to evaluate the photoprotective potential and ex vivo...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12419
更新日期：2015-05-01 00:00:00
abstract：:Antisense oligodeoxynucleotides directed against a 24-mer RNA derived from the long terminal repeat (LTR) region of HIV were linked to proto- and methylpyrroporphyrin and their zinc derivatives. The oligonucleotide-porphyrin conjugates were tested for their ability to induce photodamage on the target RNA. Upon hybridi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05110.x
更新日期：1994-10-01 00:00:00
abstract：:We present an in vivo study of the optical properties of melanin present in melanocytic nevi of human subjects with Fitzpatrick skin type III (Caucasian descent) using optical spectroscopy. We show that the melanin absorption spectrum exhibits an exponential dependence on wavelength with a decay constant which follows...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2008.00436.x
更新日期：2009-01-01 00:00:00
abstract：:Biophysical and photobiological properties of a group of bacteriochlorins were compared with efficacy of these products for photodynamic therapy of murine tumors. Predictive factors for selective photosensitization in vivo include affinity binding to lipoproteins greater than albumin, extinction coefficient at the wav...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1993.tb09549.x
更新日期：1993-08-01 00:00:00
abstract：:Catalases are oxidized by singlet oxygen giving rise to more acidic conformers detected in zymograms after electrophoresis in polyacrylamide gels. This shift in catalase mobility can be indicative of singlet oxygen production in vivo. Catalase from human cells, as from many organisms, is susceptible to in vitro modifi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Endogenous reduced nicotinamide adenine dinucleotide (NADH) fluorescence provides an intrinsic indicator of the cellular metabolic state, but prolonged monitoring is limited by photobleaching and/or phototoxicity. Multiphoton excitation of NADH by ultrashort, 740-nm laser pulses provides a significant improvement over...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-09-19-RA-689
更新日期：2006-05-01 00:00:00
abstract：:The UV radiation environment on planetary surfaces and within atmospheres is of importance in a wide range of scientific disciplines. Solar UV radiation is a driving force of chemical and organic evolution and serves also as a constraint in biological evolution. In this work we modeled the transmission of present and ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)077<0034:suicot>2.0.co;2
更新日期：2003-01-01 00:00:00
abstract：:Several studies in the literature indicate that histones (lysine rich proteins found associated with DNA in eukaryotic chromatin), as well as poly-L-lysine, can be photocross-linked by ultraviolet (UV) light to DNA in which 5-bromo-2'-deoxyuridine has been substituted for thymidine. To gain some insight into the possi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1991.tb08486.x
更新日期：1991-05-01 00:00:00
abstract：:Patients infected with human immunodeficiency virus (HIV) have a high prevalence of UV radiation-responsive skin diseases including psoriasis, pruritus, eosinophilic folliculitis and eczemas. On the other hand, UV has been shown to suppress T cell-mediated immune responses and to induce activation and replication of H...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/j.1751-1097.1996.tb02450.x
更新日期：1996-08-01 00:00:00
abstract：:Gel sequencing experiments with the 5'- and 3'-end-labeled oligonucleotides d(A3GA4GA5GA6GA3G) and d(AT)10 have demonstrated that dimeric adenine photoproducts and thymine-adenine photoadducts constitute alkali-labile lesions in UV-irradiated DNA. On treatment with hot piperidine, DNA strand breakage occurs predominan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05022.x
更新日期：1994-02-01 00:00:00
abstract：:The octahedral rhodium complex, cis-dichloro bis(1,10 phenanthroline)rhodium(III) chloride (BISPHEN), is known to form covalent linkages with DNA involving the attachment of the metal to a base. In order to determine the sequence selectivity of this chemistry, solutions of the complex containing one of the double-stra...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0731:hsawtp>2.0.co;2
更新日期：2000-12-01 00:00:00
abstract：:This paper studies the dye-sensitized photooxidation of tyrosine (tyr) and tyr di- and tripeptides (tyr-tyr and tyr-tyr-tyr) mediated by singlet molecular oxygen (O2[1 delta g]) in alkaline media. Photooxidation quantum efficiencies (phi r) were obtained by determining the overall and reactive rate constants of intera...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:A diffusible multitherapy resistance factor (MTRF) is produced by Cloudman S91 melanoma cells in vitro. The MTRF decreases sensitivity of the target cell line, S91/amel, to gamma-irradiation, UVC (200-280 nm) and mitomycin C (MMC). In the present study, we demonstrate that MTRF also increases the survival of S91/amel ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb02348.x
更新日期：1995-05-01 00:00:00
abstract：:Persister bacteria tolerate bactericidal antibiotics due to transient and reversible phenotypic changes. As these bacteria can limit the effectiveness of antibiotics to eradicate certain infections, their elimination is a relevant issue. Photodynamic therapy seems suitable for this purpose, but phenotypic tolerance to...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12843
更新日期：2018-01-01 00:00:00
abstract：:Photocatalytic degradation of methyl orange (MO) as a model of an organic pollution was accomplished with magnetic and porous TiO(2)/ZnO/Fe(3)O(4)/PANI and ZnO/Fe(3)O(4)/PANI nanocomposites under visible light irradiation. The structures of nanocomposites were characterized by various techniques including UV-Vis absor...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01209.x
更新日期：2013-01-01 00:00:00
abstract：:The modifications induced by reactive oxygen species (ROS) on fluorescent proteins (FPs) may have important implications for live cell fluorescence imaging. Using quantitative gamma-radiolysis, we have studied the ROS-induced biochemical and photophysical perturbations on recombinant cyan fluorescent protein (CFP). Af...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00617.x
更新日期：2010-01-01 00:00:00
abstract：:Cryptococus gattii is an emergent primary human pathogen that causes meningismus, papilledema, high intracranial pressure and focal involvement of the central nervous system in immunocompetent hosts. Prolonged antifungal therapy is the conventional treatment, but it is highly toxic, selects for resistant strains, cont...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00868.x
更新日期：2011-03-01 00:00:00
abstract：:The synthesis and the properties of 1,4-dibutoxy-2,3-dicyanotriptycene, of a metal-free tetradibenzobarrelenooctabutoxyphthalocyanine, and of the corresponding zinc phthalocyanine are described. The two phthalocyanines do not aggregate when dissolved in benzene at concentrations up to 450 microM. For the metal-free an...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb08510.x
更新日期：1992-05-01 00:00:00
abstract：:The incidence and prevalence of inflammatory bowel disease (IBD) are increasing worldwide. Some ecological studies show increasing incidence with increasing latitude. Ambient ultraviolet radiation varies inversely with latitude, and sun exposure of the skin is a major source of vitamin D. Vitamin D deficiency is commo...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章,评审
doi：10.1111/php.13007
更新日期：2019-01-01 00:00:00
abstract：:Psoralen is a furocoumarin natural product that intercalates within DNA and forms covalent adducts when activated by ultraviolet radiation. It is well known that this property contributes to psoralen's clinical efficacy in several disease contexts, which include vitiligo, psoriasis, graft-versus-host disease and cutan...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13263
更新日期：2020-09-01 00:00:00
abstract：:The binding interactions of three naphthalimide derivatives with heteropoly nucleic acids have been evaluated using fluorescence, absorption and circular dichroism spectroscopies. Mono- and bifunctionalized naphthalimides exhibit sequence-dependent variations in their affinity toward DNA. The heteropoly nucleic acids,...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00155.x
更新日期：2007-07-01 00:00:00
abstract：:Polysulphone (PS) dosimetry has been a widely used technique for more than 30 years to quantify the erythemally effective UV dose received by anatomic sites (personal exposure). The calibration of PS dosimeters is an important issue as their spectral response is different from the erythemal action spectrum. It is perf...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2011.01006.x
更新日期：2012-01-01 00:00:00
abstract：:Skh:HR-1 hairless mice were irradiated chronically with sub-erythemal doses of UVB radiation, and a number of biochemical parameters in the skin were determined after 6, 12, 18, and 24 wk of exposure. The parameters measured were water, collagen, elastin, and glycosaminoglycan content; collagenase and elastase levels;...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1990.tb01688.x
更新日期：1990-01-01 00:00:00
abstract：:Cyamemazine (CMZ) is a neuroleptic drug that mediates cutaneous phototoxicity in humans. Here, the photobehavior of CMZ has been examined within α1 -acid glycoproteins, β- and γ-cyclodextrins and SDS micelles. In all these microenvironments, CMZ emission was enhanced and blue-shifted, and its lifetime was longer. Irra...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12303
更新日期：2014-09-01 00:00:00
