Abstract:
:Four chemical preservatives commonly used in ophthalmic solutions were tested for their toxic and mutagenic potential in mouse lymphoma cells with and without exposure of the cells to ultraviolet A (UVA) radiation. The preservatives tested were benzalkonium chloride (BAK), chlorhexidine, thimerosal and ethylenediaminetetraacetic acid (EDTA). Cell survival and mutagenesis were measured using the L5178Y mouse lymphoma (TK +/-) system. Cells were exposed to varying amounts of preservatives for 1 h at 37 degrees C, and then aliquots were irradiated with UVA radiation (during the exposure to preservative). Cells were then assayed for survival, and for mutagenesis at the thymidine kinase (TK) locus. In concentrations commonly found in ophthalmic solutions, BAK, chlorhexidine, and thimerosal were toxic to cells, and thimerosal was slightly mutagenic. When cells were exposed to preservative and UVA radiation, chlorhexidine was mutagenic and the mutagenic activity of thimerosal was enhanced.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Withrow TJ,Brown NT,Hitchins VM,Strickland AGdoi
10.1111/j.1751-1097.1989.tb04174.xsubject
Has Abstractpub_date
1989-09-01 00:00:00pages
385-9issue
3eissn
0031-8655issn
1751-1097journal_volume
50pub_type
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