Abstract:
:The intrinsic autofluorescence properties of biological tissues can be affected by the occurrence of histological and biochemical alterations induced by pathological processes. In this study the potential of autofluorescence to distinguish tumor from normal tissues was investigated with the view of a real-time diagnostic application in neurosurgery to delineate glioblastoma resection margins. The autofluorescence properties of nonneoplastic and neoplastic tissues were analyzed on tissue sections and homogenates by means of a microspectrofluorometer, and directly on patients affected by glioblastoma multiforme, during surgery, with a fiber-optic probe. Scan-microspectrofluorometric analysis on tissue sections evidenced a reduction of emission intensity and a broadening of the main emission band, along with a redshift of the peak position, from peritumoral nonneoplastic to neoplastic tissues. Differences in both spectral shape and signal amplitude were found in patients when the glioblastoma lesion autofluorescence was compared with those of cortex and white matter taken as healthy tissues. Both biochemical composition and histological organization contribute to modify the autofluorescence emission of neoplastic, with respect to nonneoplastic, brain tissues. The differences found in the in vivo analysis confirm the prospects for improving the efficacy of tumor resection margin delineation in neurosurgery.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Croce AC,Fiorani S,Locatelli D,Nano R,Ceroni M,Tancioni F,Giombelli E,Benericetti E,Bottiroli Gdoi
10.1562/0031-8655(2003)077<0309:dpoafa>2.0.co;2subject
Has Abstractpub_date
2003-03-01 00:00:00pages
309-18issue
3eissn
0031-8655issn
1751-1097journal_volume
77pub_type
杂志文章abstract::Steady state photolysis and time resolved near infrared luminescence detection were employed to study the reaction kinetics of singlet oxygen with three different lipid-soluble probes incorporated in large unilamellar dioctadecyldimethylammonium chloride (DODAC) vesicles. The probes: 2-(4-(N,N,N-trimethylamine)-butyl)...
journal_title:Photochemistry and photobiology
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doi:10.1562/2006-08-01-RA-991
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abstract::G protein-coupled receptors (GPCRs) are involved in the control of virtually all aspects of our behavior and physiology. Activated receptors catalyze nucleotide exchange in heterotrimeric G proteins (composed of alpha.GDP, beta and gamma subunits) on the inner surface of the cell membrane. The GPCR rhodopsin and the G...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00304.x
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
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doi:10.1111/j.1751-1097.2012.01188.x
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2012.01105.x
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-03-17-RA-117
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb04930.x
更新日期:1993-10-01 00:00:00
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00393.x
更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2011-09-01 00:00:00
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journal_title:Photochemistry and photobiology
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doi:
更新日期:2002-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2011.01006.x
更新日期:2012-01-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13085
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
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更新日期:2008-11-01 00:00:00
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journal_title:Photochemistry and photobiology
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
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更新日期:2006-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.13348
更新日期:2020-10-24 00:00:00