Abstract:
:The mode of cell death following photodynamic therapy was investigated from the perspective of programmed cell death or apoptosis. Human prostate carcinoma cells (PC3), human non-small cell lung carcinoma (H322a) and rat mammary carcinoma (MTF7) were treated by photodynamic therapy. An examination of extracted cellular DNA by gel electrophoresis showed the characteristic DNA ladder indicative of internucleosomal cleavage of DNA during apoptosis. The magnitude of the response and the photodynamic therapy dosage required to induce DNA fragmentation were different in PC3 and MTF7. The MTF7 cells responded with rapid apoptosis at the dose of light and drug that yielded 50% cell death (LD50). In contrast, PC3 showed only marginal response at the LD50 but had a marked response at the LD85. Thus, apoptosis did not ensue as quickly in PC3 as in MTF7. The H322a cells were killed by photodynamic therapy but failed to exhibit any apoptotic response. The results also suggested that apoptosis in these cell lines has a minor requirement for de novo protein synthesis and no requirement for de novo RNA synthesis. This study indicates that although apoptosis can occur during photodynamic therapy-induced cell death, this response is not universal for all cancer cell lines.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
He XY,Sikes RA,Thomsen S,Chung LW,Jacques SLdoi
10.1111/j.1751-1097.1994.tb05066.xsubject
Has Abstractpub_date
1994-04-01 00:00:00pages
468-73issue
4eissn
0031-8655issn
1751-1097journal_volume
59pub_type
杂志文章abstract::We have been able to identify a blue fluorophore from the low-molecular weight soluble fraction of human adult nondiabetic brunescent cataract lenses as xanthurenic acid 8-O-beta-D-glucoside (XA8OG) (excitation = 338 nm and emission = 440 nm). To determine the role of this fluorophore in the lens, we have examined its...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)076<0368:roxaod>2.0.co;2
更新日期:2002-09-01 00:00:00
abstract::We have studied the damage of alcohol dehydrogenase (ADH) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) induced by Fe++/EDTA + H2O2 in combination with UV-A (main output at 365 nm). Enzyme inactivation, formation of hydroxyl radicals (measured in the absence of enzymes), increase in protein carbonyls, oxidation ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1992.tb02171.x
更新日期:1992-09-01 00:00:00
abstract::A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results sugge...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1992.tb02227.x
更新日期:1992-11-01 00:00:00
abstract::The photocycle in photoactive yellow protein (PYP) crystals was studied by single-crystal absorption spectroscopy with experimental setups for low-temperature and time-resolved measurements. Thin and flat PYP crystals, suitable for light absorption studies, were obtained using special crystallization conditions. Illum...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)078<0131:copiic>2.0.co;2
更新日期:2003-08-01 00:00:00
abstract::The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity wa...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1995.tb03948.x
更新日期:1995-02-01 00:00:00
abstract::Photodynamic therapy (PDT), carried out at low fluence rates, may enhance tumor response as well as affect treatment selectivity. We have studied the effects of fluence rate on the response of the murine radiation-induced fibrosarcoma (RIF) to PDT using Photofrin (5 mg/kg). Tumor response was tested over a large range...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章,评审
doi:10.1111/php.12716
更新日期:2017-07-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13231
更新日期:2020-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12836
更新日期:2018-01-01 00:00:00
abstract::A 2-year field study on the effect of different light environments, obtained by using cladding materials (polyethylene films and shade net) able to cut off specific regions of the photosynthetically active radiation and ultraviolet wavebands, on the growth and phenylpropanoids content of Achillea collina grown in the ...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12150
更新日期:2014-01-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.13183
更新日期:2020-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1990.tb08674.x
更新日期:1990-10-01 00:00:00
abstract::A new water-soluble porphyrin derivative, 2,4-bis(1-decyloxyethyl)-deuteroporphyrinyl-6,7-bisaspart ic acid (C10-DP), and its metal complexes (Ga, I(n), Zn, Mn, Cu, Ni and Fe) were examined for their physicochemical properties (absorption, fluorescence, triplet lifetime and partition coefficient) and photocytotoxicity...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1993.tb02928.x
更新日期:1993-04-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2004-04-22-RA-147
更新日期:2004-09-01 00:00:00
abstract::Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects o...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:
更新日期:1998-05-01 00:00:00
abstract::International patterns suggest germ cell testicular cancer (GCTC) incidence may be lower in lower latitudes. To investigate this possibility, we examined GCTC incidence by latitude (population centroid in 2000) for men ≥15 years within two reasonably homogeneous countries, the United States and Australia. In the Unite...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12617
更新日期:2016-09-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/php.12144
更新日期:2013-11-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.1989.tb08436.x
更新日期:1989-05-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2002)075<0126:puorcr>2.0.co;2
更新日期:2002-02-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)071<0737:miamft>2.0.co;2
更新日期:2000-06-01 00:00:00
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journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2000)071<0321:pduroh>2.0.co;2
更新日期:2000-03-01 00:00:00
abstract::We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHC...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2010.00830.x
更新日期:2011-01-01 00:00:00
abstract::It has been proposed that photodegradation of folates may be the reason for the pigmentation of races living under high fluence rates of ultraviolet radiation. The photodegradation of folic acid (FA) induced by ultraviolet-A (UV-A) radiation, in solution and in the presence of human serum albumin (HSA), was studied wi...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/2005-11-23-RA-739
更新日期:2006-05-01 00:00:00
abstract::We conducted a thermovisual comparison of mean hand surface temperature changes upon local heating with two different IR sources. Sixty-six patients with rheumatoid arthritis (47 women and 19 men; average age, 56.1 ± 8.6 years) were subjected to topical heat therapy for one hand with either the standard IR radiator (S...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2011.00975.x
更新日期:2011-11-01 00:00:00
abstract::The photophysical properties of two energy-transfer dyads that are potential candidates for near-infrared (NIR) imaging probes are investigated as a function of solvent polarity. The dyads (FbC-FbB and ZnC-FbB) contain either a free base (Fb) or zinc (Zn) chlorin (C) as the energy donor and a free base bacteriochlorin...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2008.00532.x
更新日期:2009-07-01 00:00:00
abstract::Cyanines are emerging as useful agents for photoreleasing biological compounds because of their capability of utilizing near-infrared (NIR) light. Another benefit is their ability to self-sensitize to produce singlet oxygen for the release of aryl amines, a process that has not been as feasible in the past. Here, we h...
journal_title:Photochemistry and photobiology
pub_type: 评论,杂志文章
doi:10.1111/php.13102
更新日期:2019-09-01 00:00:00
abstract::Dark-grown leaves of maize (Zea mays), wheat (Triticum aestivum), wild-type pea (Pisum sativum) and its light-independent photomorphogenesis mutant (lip1) have different proportions of protochlorophyllide (Pchlide) forms as revealed by low-temperature fluorescence emission spectra. Four discrete spectral forms of Pchl...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2003)078<0205:flopip>2.0.co;2
更新日期:2003-08-01 00:00:00
abstract::This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1111/j.1751-1097.2009.00570.x
更新日期:2009-09-01 00:00:00
abstract::Low-power lasers are commonly used in human medicine for treatment of various pathological conditions, but mechanisms of their healing effects are still poorly understood. The results of this study provide information related to these effects at the cellular level. Two different protozoan species, Euglena gracilis and...
journal_title:Photochemistry and photobiology
pub_type: 杂志文章
doi:10.1562/0031-8655(2004)080<0531:EOLLIO>2.0.CO;2
更新日期:2004-11-01 00:00:00