Photodynamic therapy with photofrin II induces programmed cell death in carcinoma cell lines.

Abstract:

:The mode of cell death following photodynamic therapy was investigated from the perspective of programmed cell death or apoptosis. Human prostate carcinoma cells (PC3), human non-small cell lung carcinoma (H322a) and rat mammary carcinoma (MTF7) were treated by photodynamic therapy. An examination of extracted cellular DNA by gel electrophoresis showed the characteristic DNA ladder indicative of internucleosomal cleavage of DNA during apoptosis. The magnitude of the response and the photodynamic therapy dosage required to induce DNA fragmentation were different in PC3 and MTF7. The MTF7 cells responded with rapid apoptosis at the dose of light and drug that yielded 50% cell death (LD50). In contrast, PC3 showed only marginal response at the LD50 but had a marked response at the LD85. Thus, apoptosis did not ensue as quickly in PC3 as in MTF7. The H322a cells were killed by photodynamic therapy but failed to exhibit any apoptotic response. The results also suggested that apoptosis in these cell lines has a minor requirement for de novo protein synthesis and no requirement for de novo RNA synthesis. This study indicates that although apoptosis can occur during photodynamic therapy-induced cell death, this response is not universal for all cancer cell lines.

journal_name

Photochem Photobiol

authors

He XY,Sikes RA,Thomsen S,Chung LW,Jacques SL

doi

10.1111/j.1751-1097.1994.tb05066.x

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

468-73

issue

4

eissn

0031-8655

issn

1751-1097

journal_volume

59

pub_type

杂志文章
  • Role of xanthurenic acid 8-O-beta-D-glucoside, a novel fluorophore that accumulates in the brunescent human eye lens.

    abstract::We have been able to identify a blue fluorophore from the low-molecular weight soluble fraction of human adult nondiabetic brunescent cataract lenses as xanthurenic acid 8-O-beta-D-glucoside (XA8OG) (excitation = 338 nm and emission = 440 nm). To determine the role of this fluorophore in the lens, we have examined its...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2002)076<0368:roxaod>2.0.co;2

    authors: Thiagarajan G,Shirao E,Ando K,Inoue A,Balasubramanian D

    更新日期:2002-09-01 00:00:00

  • Potentiation of oxidative damage to proteins by ultraviolet-A and protection by antioxidants.

    abstract::We have studied the damage of alcohol dehydrogenase (ADH) and glyceraldehyde 3-phosphate dehydrogenase (GAPD) induced by Fe++/EDTA + H2O2 in combination with UV-A (main output at 365 nm). Enzyme inactivation, formation of hydroxyl radicals (measured in the absence of enzymes), increase in protein carbonyls, oxidation ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1992.tb02171.x

    authors: Hu ML,Tappel AL

    更新日期:1992-09-01 00:00:00

  • The distance between the phytochrome chromophore and the N-terminal chain decreases during phototransformation. A novel fluorescence energy transfer method using labeled antibody fragments.

    abstract::A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results sugge...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1992.tb02227.x

    authors: Farrens DL,Cordonnier MM,Pratt LH,Song PS

    更新日期:1992-11-01 00:00:00

  • Characterization of photocycle intermediates in crystalline photoactive yellow protein.

    abstract::The photocycle in photoactive yellow protein (PYP) crystals was studied by single-crystal absorption spectroscopy with experimental setups for low-temperature and time-resolved measurements. Thin and flat PYP crystals, suitable for light absorption studies, were obtained using special crystallization conditions. Illum...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)078<0131:copiic>2.0.co;2

    authors: Kort R,Ravelli RB,Schotte F,Bourgeois D,Crielaard W,Hellingwerf KJ,Wulff M

    更新日期:2003-08-01 00:00:00

  • Sequence specificity of tetrahydrobenzopsoralen photobinding to DNA.

    abstract::The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity wa...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1995.tb03948.x

    authors: Mobilio S,Tondelli L,Capobianco M,Gia O

    更新日期:1995-02-01 00:00:00

  • The effect of fluence rate on tumor and normal tissue responses to photodynamic therapy.

    abstract::Photodynamic therapy (PDT), carried out at low fluence rates, may enhance tumor response as well as affect treatment selectivity. We have studied the effects of fluence rate on the response of the murine radiation-induced fibrosarcoma (RIF) to PDT using Photofrin (5 mg/kg). Tumor response was tested over a large range...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Sitnik TM,Henderson BW

    更新日期:1998-04-01 00:00:00

  • Type I and Type II Photosensitized Oxidation Reactions: Guidelines and Mechanistic Pathways.

    abstract::Here, 10 guidelines are presented for a standardized definition of type I and type II photosensitized oxidation reactions. Because of varied notions of reactions mediated by photosensitizers, a checklist of recommendations is provided for their definitions. Type I and type II photoreactions are oxygen-dependent and in...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章,评审

    doi:10.1111/php.12716

    authors: Baptista MS,Cadet J,Di Mascio P,Ghogare AA,Greer A,Hamblin MR,Lorente C,Nunez SC,Ribeiro MS,Thomas AH,Vignoni M,Yoshimura TM

    更新日期:2017-07-01 00:00:00

  • Quantum yields of triplet and O2(1 delta g) formation of 4-thiouridine in water and acetonitrile.

    abstract::The quantum yield of triplet formation, phi T, and that of the photosensitized formation of singlet molecular oxygen, phi delta, were determined for a rare nucleoside, 4-thiouridine (4t-Urd), in water and in acetonitrile, using singlet molecular oxygen phosphorescence, laser-induced optoacoustics and time-resolved the...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Heihoff K,Redmond RW,Braslavsky SE,Rougée M,Salet C,Favre A,Bensasson RV

    更新日期:1990-06-01 00:00:00

  • Site-specific Bioconjugation and Convergent Click Chemistry Enhances Antibody-Chromophore Conjugate Binding Efficiency.

    abstract::Photosensitizer (PS)-antibody conjugates (photoimmunoconjugates, PICs) enable cancer cell-targeted photodynamic therapy (PDT). Nonspecific chemical bioconjugation is widely used to synthesize PICs but gives rise to several shortcomings. The conjugates are heterogeneous, and the process is not easily reproducible. More...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13231

    authors: Sadiki A,Kercher EM,Lu H,Lang RT,Spring BQ,Zhou ZS

    更新日期:2020-05-01 00:00:00

  • Facile Fabrication of Porous ZnS and ZnO Films by Coaxial Electrospinning for Highly Efficient Photodegradation of Organic Dyes.

    abstract::Porous ZnS and ZnO nano-crystal films were fabricated via a three-step procedure. First, Zn(CH3 COO)2 /Silk Fibroin nanofiber mats were prepared by coaxial electrospinning. Second, Zn(CH3 COO)2 /Silk Fibroin mats were immersed in NaS solution to react with S2- to obtain ZnS/Silk Fibroin nanofiber mats. Finally, ZnO po...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12836

    authors: Wang H,Liang L,Cheng X,Luo Y,Sun S

    更新日期:2018-01-01 00:00:00

  • Effect of Light Environment on Growth and Phenylpropanoids of Yarrow (Achillea collina cv. SPAK) Grown in the Alps.

    abstract::A 2-year field study on the effect of different light environments, obtained by using cladding materials (polyethylene films and shade net) able to cut off specific regions of the photosynthetically active radiation and ultraviolet wavebands, on the growth and phenylpropanoids content of Achillea collina grown in the ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12150

    authors: Giorgi A,Manzo A,Vagge I,Panseri S

    更新日期:2014-01-01 00:00:00

  • Pluronic F-127: An Efficient Delivery Vehicle for 3-(1'-hexyloxy)ethyl-3-devinylpyropheophorbide-a (HPPH or Photochlor).

    abstract::To determine the impact of delivery vehicles in photosensitizing efficacy of HPPH, a hydrophobic photosensitizer was dissolved in various formulations: 1% Tween 80/5% dextrose, Pluronic P-123 and Pluronic F-127 in 0.5%, 1% and 2% phosphate buffer solutions (PBS). HPPH was also conjugated to Pluronic F-127, and the res...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.13183

    authors: Cacaccio J,Durrani F,Cheruku RR,Borah B,Ethirajan M,Tabaczynski W,Pera P,Missert JR,Pandey RK

    更新日期:2020-05-01 00:00:00

  • In vitro photodynamic activity of kryptocyanine.

    abstract::The photophysical properties of 1,1'-diethyl-4,4'-carbocyanine chloride (kryptocyanine) have been measured in methanol solution and for the dye bound to human serum albumin, incorporated in neutral micelles and after incubation with leukemia cells. In all cases, it is found that formation of the triplet state of the d...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1990.tb08674.x

    authors: Harriman A,Luengo G,Gulliya KS

    更新日期:1990-10-01 00:00:00

  • Photocytotoxicity of water-soluble metalloporphyrin derivatives.

    abstract::A new water-soluble porphyrin derivative, 2,4-bis(1-decyloxyethyl)-deuteroporphyrinyl-6,7-bisaspart ic acid (C10-DP), and its metal complexes (Ga, I(n), Zn, Mn, Cu, Ni and Fe) were examined for their physicochemical properties (absorption, fluorescence, triplet lifetime and partition coefficient) and photocytotoxicity...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1993.tb02928.x

    authors: Ando T,Irie K,Koshimizu K,Takemura T,Nishino H,Iwashima A,Takeda N,Nakajima S,Sakata I

    更新日期:1993-04-01 00:00:00

  • Radical cation generation from singlet and triplet excited states of all-trans-lycopene in chloroform.

    abstract::On direct photoexcitation, subpicosecond time-resolved absorption spectroscopy revealed that the 1B(u)-type singlet excited state of all-trans-lycopene in chloroform was about seven times more efficient than all-trans-beta-carotene in generating the radical cation. The time constant of radical cation generation from t...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2004-04-22-RA-147

    authors: Han RM,Wu YS,Feng J,Ai XC,Zhang JP,Skibsted LH

    更新日期:2004-09-01 00:00:00

  • Characterization of endogenous protoporphyrin IX induced by delta-aminolevulinic acid in resting and activated peripheral blood lymphocytes by four-color flow cytometry.

    abstract::Lymphocytes treated with delta-aminolevulinic acid (ALA) can accumulate the photoactive, fluorescent heme precursor, protoporphyrin IX (PpIX). With visible light illumination, PpIX can be used in photodynamic therapy (ALA-PDT) to kill or functionally alter cells. The aim of this study was to characterize the effects o...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:

    authors: Hryhorenko EA,Rittenhouse-Diakun K,Harvey NS,Morgan J,Stewart CC,Oseroff AR

    更新日期:1998-05-01 00:00:00

  • Germ Cell Testicular Cancer Incidence, Latitude and Sunlight Associations in the United States and Australia.

    abstract::International patterns suggest germ cell testicular cancer (GCTC) incidence may be lower in lower latitudes. To investigate this possibility, we examined GCTC incidence by latitude (population centroid in 2000) for men ≥15 years within two reasonably homogeneous countries, the United States and Australia. In the Unite...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12617

    authors: Biggar RJ,Baade PD,Sun J,Brandon LE,Kimlin M

    更新日期:2016-09-01 00:00:00

  • Solar UV exposure of seafarers along subtropical and tropical shipping routes.

    abstract::Seafarers working on decks of vessels at low latitudes are exposed to extremely high solar UV radiation. Their risk of developing skin cancer may be enhanced. Solar erythemal UV irradiance and exposure were measured for the first time on merchant vessels going along typical international routes at low latitudes. The m...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/php.12144

    authors: Feister U,Meyer G,Kirst U

    更新日期:2013-11-01 00:00:00

  • Interaction of dibucaine.HCl local anesthetics with bacteriorhodopsin in purple membrane: a spectroscopic study.

    abstract::Several spectroscopic techniques (absorption, emission, transient absorption and differential scanning calorimetry--DSC) were used to investigate the deprotonation of dibucaine.HCl in a hydrophobic environment, and the interaction sites and mechanisms of the local anesthetic dibucaine.HCl on bacteriorhodopsin (bR) in ...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.1989.tb08436.x

    authors: Lin CT,Chyan YG,Kresheck GC,Bitting HC Jr,el-Sayed MA

    更新日期:1989-05-01 00:00:00

  • Proton uptake of rhodobacter capsulatus reaction center mutants modified in the primary quinone environment.

    abstract::Flash-induced absorbance spectroscopy was used to analyze the proton uptake and electron transfer properties of photosynthetic reaction centers (RC) of Rhodobacter capsulatus that have been genetically modified near the primary quinone electron acceptor (Q(A)). M246Ala and M247Ala, which are symmetry-related to the po...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2002)075<0126:puorcr>2.0.co;2

    authors: Tandori J,Miksovska J,Valerio-Lepiniec M,Schiffer M,Maróti P,Hanson DK,Sebban P

    更新日期:2002-02-01 00:00:00

  • Multispectral imaging autofluorescence microscopy for the analysis of lymph-node tissues.

    abstract::Although histochemical and immunohistochemical methods are the standard procedures in diagnosis of lymphoproliferative disorders, useful improvements in evidencing histopathologic manifestations can be obtained with the introduction of tissue autofluorescence analyses. We used microspectrofluorometry and a Multispectr...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0737:miamft>2.0.co;2

    authors: Rigacci L,Alterini R,Bernabei PA,Ferrini PR,Agati G,Fusi F,Monici M

    更新日期:2000-06-01 00:00:00

  • P53-dependent UVB responsiveness of human keratinocytes can be altered by cultivation on cell cycle-arrested dermal fibroblasts.

    abstract::In cultured human keratinocytes, the tumor suppressor p53 acts as a control element in the protective response to UVB radiation and is affected by a variety of factors linked to cellular adhesion and differentiation. Because keratinocytes within the epidermis are not a homogeneous population but differ in their prolif...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2000)071<0321:pduroh>2.0.co;2

    authors: Will K,Neben M,Schmidt-Rose T,Deppert W,Wittern KP,Bergemann J

    更新日期:2000-03-01 00:00:00

  • Isolation and characteristics of the PSI-LHCI-LHCII supercomplex under high light.

    abstract::We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHC...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2010.00830.x

    authors: Qin X,Wang W,Wang K,Xin Y,Kuang T

    更新日期:2011-01-01 00:00:00

  • Influence of human serum albumin on photodegradation of folic acid in solution.

    abstract::It has been proposed that photodegradation of folates may be the reason for the pigmentation of races living under high fluence rates of ultraviolet radiation. The photodegradation of folic acid (FA) induced by ultraviolet-A (UV-A) radiation, in solution and in the presence of human serum albumin (HSA), was studied wi...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/2005-11-23-RA-739

    authors: Vorobey P,Steindal AE,Off MK,Vorobey A,Moan J

    更新日期:2006-05-01 00:00:00

  • Thermal effectiveness of different IR radiators employed in rheumatoid hand therapy as assessed by thermovisual examination.

    abstract::We conducted a thermovisual comparison of mean hand surface temperature changes upon local heating with two different IR sources. Sixty-six patients with rheumatoid arthritis (47 women and 19 men; average age, 56.1 ± 8.6 years) were subjected to topical heat therapy for one hand with either the standard IR radiator (S...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2011.00975.x

    authors: Rutkowski R,Straburzyńska-Lupa A,Korman P,Romanowski W,Gizińska M

    更新日期:2011-11-01 00:00:00

  • Chlorin-bacteriochlorin energy-transfer dyads as prototypes for near-infrared molecular imaging probes: controlling charge-transfer and fluorescence properties in polar media.

    abstract::The photophysical properties of two energy-transfer dyads that are potential candidates for near-infrared (NIR) imaging probes are investigated as a function of solvent polarity. The dyads (FbC-FbB and ZnC-FbB) contain either a free base (Fb) or zinc (Zn) chlorin (C) as the energy donor and a free base bacteriochlorin...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2008.00532.x

    authors: Kee HL,Diers JR,Ptaszek M,Muthiah C,Fan D,Lindsey JS,Bocian DF,Holten D

    更新日期:2009-07-01 00:00:00

  • Cyanine Modification Tuned for Amine Photorelease.

    abstract::Cyanines are emerging as useful agents for photoreleasing biological compounds because of their capability of utilizing near-infrared (NIR) light. Another benefit is their ability to self-sensitize to produce singlet oxygen for the release of aryl amines, a process that has not been as feasible in the past. Here, we h...

    journal_title:Photochemistry and photobiology

    pub_type: 评论,杂志文章

    doi:10.1111/php.13102

    authors: Mathew JP,Greer A

    更新日期:2019-09-01 00:00:00

  • Fluorescence lifetimes of protochlorophyllide in plants with different proportions of short-wavelength and long-wavelength protochlorophyllide spectral forms.

    abstract::Dark-grown leaves of maize (Zea mays), wheat (Triticum aestivum), wild-type pea (Pisum sativum) and its light-independent photomorphogenesis mutant (lip1) have different proportions of protochlorophyllide (Pchlide) forms as revealed by low-temperature fluorescence emission spectra. Four discrete spectral forms of Pchl...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2003)078<0205:flopip>2.0.co;2

    authors: Myśliwa-Kurdziel B,Amirjani MR,Strzałka K,Sundqvist C

    更新日期:2003-08-01 00:00:00

  • Infrared radiation influence on molt and regeneration of Neohelice granulata Dana, 1851 (Grapsidae, Sesarminae).

    abstract::This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups....

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1111/j.1751-1097.2009.00570.x

    authors: Gonzalez VC,Lena AB,Espindola WA,de Souza Votto AP,Jorge MB,de Moraes Vaz Batista Filgueira D,Bianchini A,Sérgio Varela Júnior A,Nery LE,Trindade GS

    更新日期:2009-09-01 00:00:00

  • Effects of low-power laser irradiation on cell locomotion in protozoa.

    abstract::Low-power lasers are commonly used in human medicine for treatment of various pathological conditions, but mechanisms of their healing effects are still poorly understood. The results of this study provide information related to these effects at the cellular level. Two different protozoan species, Euglena gracilis and...

    journal_title:Photochemistry and photobiology

    pub_type: 杂志文章

    doi:10.1562/0031-8655(2004)080<0531:EOLLIO>2.0.CO;2

    authors: Koutna M,Janisch R,Unucka M,Svobodnik A,Mornstein V

    更新日期:2004-11-01 00:00:00