Isolation and characteristics of the PSI-LHCI-LHCII supercomplex under high light.

Abstract:

:We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHCII under high light. In addition, we compared light-induced denaturation of the core protein subunits in both PSI-LHCI and PSI-LHCI-LHCII. Differences in denaturation and photochemical activity indicated that binding of LHCII increased the photosensitivity of the PSI core. Increased energy delivered to the PSI core during illumination accelerated damage to the core complex.

journal_name

Photochem Photobiol

authors

Qin X,Wang W,Wang K,Xin Y,Kuang T

doi

10.1111/j.1751-1097.2010.00830.x

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

143-50

issue

1

eissn

0031-8655

issn

1751-1097

journal_volume

87

pub_type

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