Abstract:
:We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHCII under high light. In addition, we compared light-induced denaturation of the core protein subunits in both PSI-LHCI and PSI-LHCI-LHCII. Differences in denaturation and photochemical activity indicated that binding of LHCII increased the photosensitivity of the PSI core. Increased energy delivered to the PSI core during illumination accelerated damage to the core complex.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Qin X,Wang W,Wang K,Xin Y,Kuang Tdoi
10.1111/j.1751-1097.2010.00830.xsubject
Has Abstractpub_date
2011-01-01 00:00:00pages
143-50issue
1eissn
0031-8655issn
1751-1097journal_volume
87pub_type
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