Abstract：
:The design, synthesis and application of a new fluorescent-labeling reagent for collagen has been developed as a prerequisite for the design of a photoactivated collagen-crosslinking compound for surgical wound closure. The amine groups in collagen are the targets of a rational design for a new fluorophore because natural collagen crosslinks are formed between primary (1(o)) amine groups of lysine and hydroxylysine. The availability of 1(o) amines for crosslinking in native collagenous tissues was evaluated by reacting tendon and corneal samples with o-phthalaldehyde and dansyl chloride, fluorophores commonly used for the detection of 1(o) and 2(o) amines. The resulting fluorescent collagen fibrils indicated the presence of amines in native tissue. Subsequently, a photoactivated fluorescent label for 1(o) and 2(o) amines, coumaryl gamma-amino-butyric acid diazopyruvate (CGDP), was designed and synthesized. CGDP was first used to photolabel poly-L-lysine, forming a fluorescent, covalent bond to the 1(o) amine. CGDP was then photoreacted with corneal and tendon tissue samples to produce CGDP fluorescent-labeled samples that were statistically significantly more fluorescent than were the controls. These experiments support the postulate that 1(o) or 2(o) (or both) amines in native collagenous tissues are available to serve as targets for photoactivated collagen crosslinkers for wound closure.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Timberlake GT,Reddy GK,Stehno-Bittel L,Weber JF,Amslinger S,Givens RSdoi
10.1562/0031-8655(2002)076<0473:pcdflf>2.0.co;2subject
Has Abstractpub_date
2002-11-01 00:00:00pages
473-9issue
5eissn
0031-8655issn
1751-1097journal_volume
76pub_type
杂志文章abstract：:The ultraviolet (UV) component of solar radiation is the driving force of life on earth, but it can cause photoaging and skin cancer. In this study, we investigated the effects of the glucosamine-derivative 2-(N-Acetyl)-L-phenylalanylamido-2-deoxy-β-D-glucose (NAPA) on human primary fibroblasts (FBs) stimulated in vit...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13185
更新日期：2020-01-01 00:00:00
abstract：:Urinary cytology is a noninvasive and unconstraining technique for urothelial cancer diagnosis but lacks sensitivity for detecting low-grade lesions. In this study, the fluorescence properties of classical Papanicolaou-stained urothelial cytological slides from patients or from cell lines were monitored to investigate...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00079.x
更新日期：2007-09-01 00:00:00
abstract：:The ciliate Stentor coeruleus exhibits photodispersal, that is, these cells swim away from light sources and collect in dimly lighted areas. We imaged and reconstructed the tracks of 48 Stentor to determine which swimming behaviors produced their photodispersal. We observed that their photodispersal is not due to a ch...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2004)080<0401:BPPISC>2.0.CO;2
更新日期：2004-11-01 00:00:00
abstract：:Phage T7 can be used as a biological UV dosimeter. Its reading is proportional to the inactivation rate expressed in HT7 units. To understand the influence of phage proteins on the formation of DNA UV photoproducts, cyclobutane pyrimidine dimers (CPD) and (6-4)photoproducts ((6-4)PD) were determined in T7 DNA exposed ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:UV-induced toxicity is characterized by marked oxidative stress, accompanied by the depletion of key cellular antioxidants, particularly glutathione (GSH). Replenishing cellular GSH may represent a means of counteracting UV-induced toxicity: however, treatment with free GSH is not therapeutically effective due to its ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2012.01231.x
更新日期：2013-03-01 00:00:00
abstract：:An approximate formula for the UV Index (UVI) under cloud-free, unpolluted, low surface albedo conditions is: UVI approximately 12.5mu(o)(2.42)(Omega/300)(-1.23) where mu(o) is the cosine of the solar zenith angle and Omega is the total vertical ozone column (in Dobson Units, DU). The dependence on mu(o) and Omega is ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2007.00200.x
更新日期：2007-11-01 00:00:00
abstract：:The photostability of drugs administered topically on unprotected skin is a complex phenomenon that could be connected with the loss of activity or, rather rarely, the occurrence of toxic degradation products. In this study, an in-depth investigation of the photostability of terbinafine, in both solutions and formulat...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13075
更新日期：2019-07-01 00:00:00
abstract：:Pyocyanin (PyO-) (1-hydroxy-5-methylphenazine) is a cytotoxic compound secreted by Pseudomonas aeruginosa, an omnipresent bacterium and a human pathogen. We report that visible light illumination in the presence of rose bengal, or riboflavin, in aerated solutions (pH 7.0-7.2) induces irreversible loss of the pigment's...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2005-07-29-RA-626
更新日期：2006-03-01 00:00:00
abstract：:Quantum yields for the formation of superoxide ions, O2-., and singlet oxygen, 1O2, were determined during the photolyses of gilvocarcin M (GM) in air-saturated dry dimethylsulfoxide (DMSO) and in 45:55 (vol/vol) DMSO-water mixtures. The quantum yield for the photoreduction of methyl viologen by GM in nitrogen-saturat...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1995.tb02361.x
更新日期：1995-09-01 00:00:00
abstract：:2-Aminoacetophenone (AAP) was recognized as the key compound for the so-called untypical aging off-flavor (UTA) in Vitis vinifera wines. In this study, it was shown that AAP can be formed by photooxidation of free and protein-bound tryptophan (TRP) in combination with a subsequent storage in model wine. Solutions of T...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12321
更新日期：2014-11-01 00:00:00
abstract：:A careful study of the linoleic acid hydroperoxide (LOOH) profile obtained upon peroxidation of linoleic acid (LA) photosensitized by tiaprofenic acid (TPA) and analogous ketones has been undertaken to distinguish between type-I and type-II photoperoxidation mechanisms. 1,4-Cyclohexadiene and 1,2-dimethylcyclohexa-2,5...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0359:molppb>2.0.co;2
更新日期：2001-04-01 00:00:00
abstract：:In current clinical practice, photodynamic therapy (PDT) is carried out with prescribed drug doses and light doses as well as fixed drug-light intervals and illumination fluence rates. This approach can result in undesirable treatment outcomes of either overtreatment or undertreatment because of biological variations ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)075<0398:mpfapb>2.0.co;2
更新日期：2002-04-01 00:00:00
abstract：:Ring-fused retinal analogs were designed to examine the hula-twist mode of the photoisomerization of the 9-cis retinylidene chromophore. Two 9-cis retinal analogs, the C11-C13 five-membered ring-fused and the C12-C14 five-membered ring-fused retinal derivatives, formed the pigments with opsin. The C11-C13 ring-fused a...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2002)076<0606:coosot>2.0.co;2
更新日期：2002-12-01 00:00:00
abstract：:Normal hematopoietic progenitor cells from 129S6/SvEv mice are substantially less sensitive to Merocyanine 540 (MC540)-mediated photodynamic therapy (PDT) than hematopoietic progenitors from sex- and age-matched C57BL/6 mice. When exposed to a combination of MC540 and light commonly used for the extracorporeal purging...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2000)072<0810:gvitro>2.0.co;2
更新日期：2000-12-01 00:00:00
abstract：:This study compares the abilities of the glutathione (GSH) and thioredoxin (Trx) antioxidant systems in defending cultured human lens epithelial cells (LECs) against UVA light. Levels of GSH were depleted with either L-buthionine-(S,R)-sulfoximine (BSO) or 1-chloro-2,4-dinitrobenzene (CDNB). CDNB treatment also inhibi...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12404
更新日期：2015-03-01 00:00:00
abstract：:A novel Cs0.33 WO3 /LDHs (CWLDH) composite was synthesized by simple two steps solvothermal method and first investigated as the photocatalyst for tetracycline (TC) and Congo red (CR) degradation under visible light irradiation. The CWLDH heterostructures catalysts were characterized by XRD, UV-Vis, SEM, XPS and BET. ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12838
更新日期：2018-03-01 00:00:00
abstract：:N2-hydroxyisocytosine and 1-methyl-N2-hydroxyisocytosine were studied using a matrix isolation technique combined with infrared absorption spectroscopy. For N2-hydroxyisocytosine isolated in an Ar matrix (at 10 K), two imino-oxo isomers, one with the hydroxyimino =N-OH group directed toward the N1-H group (the form ca...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2003)077<0243:psairi>2.0.co;2
更新日期：2003-03-01 00:00:00
abstract：:Photoactive materials based on dye molecules incorporated into thin films or bulk solids are useful for applications as photosensitization, photocatalysis, solar cell sensitization and fluorescent labeling, among others. In most cases, high concentrations of dyes are desirable to maximize light absorption. Under these...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12107
更新日期：2013-11-01 00:00:00
abstract：:This paper focuses on the estimation of the UV Index (UVI) for all sky conditions using a simple analytical parameterization involving three independent variables: the solar zenith angle, the total ozone column and the clearness index. Measurements of the UVI made at Badajoz and Cáceres (Southwestern Spain) from Janua...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2010.00860.x
更新日期：2011-03-01 00:00:00
abstract：:Cyanines are emerging as useful agents for photoreleasing biological compounds because of their capability of utilizing near-infrared (NIR) light. Another benefit is their ability to self-sensitize to produce singlet oxygen for the release of aryl amines, a process that has not been as feasible in the past. Here, we h...
journal_title：Photochemistry and photobiology
pub_type： 评论,杂志文章
doi：10.1111/php.13102
更新日期：2019-09-01 00:00:00
abstract：:The crystal and molecular structure of the title compound 4 have been determined by single crystal X-ray crystallography. The structure shows the typical S4-ruffled conformation observed for Ni(II) tetrapyrroles. Compared to the structure of the methyl ester of (13(2)-demethoxycarbonyl-pheophorbidato a)nickel(II), 4 s...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05080.x
更新日期：1994-08-01 00:00:00
abstract：:The objective of this study was to assess the diagnostic potential of synchronous fluorescence (SF) spectroscopy (SFS) technique for the detection and characterization of normal and different malignancy stages of moderately differentiated squamous cell carcinoma (MDSCC), poorly differentiated squamous cell carcinoma (...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00628.x
更新日期：2010-01-01 00:00:00
abstract：:Cypridina luciferase (Cluc), a secreted luminescent protein identified from Cypridina noctiluca, has two N-glycosylation sites. In this study, we evaluated the effects of N-glycosylation on Cluc properties by creating site-directed mutagenic modifications at the consensus sequence for N-glycosylation (Asn-X-Ser/Thr). ...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12847
更新日期：2018-03-01 00:00:00
abstract：:A novel laser system has been developed to study the effects of multiple laser pulses of differing wavelengths on cutaneous blood vessels in vivo, using the hamster dorsal skin flap preparation and in vitro, using cuvettes of whole or diluted blood. The system permits sequenced irradiation with well-defined intrapulse...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/0031-8655(2001)073<0642:cpitpt>2.0.co;2
更新日期：2001-06-01 00:00:00
abstract：:A solution of iodouracil (0.2 mg/mL) and KI (0.1 M) in 0.23 M borate buffer (pH 7) forms triiodide upon exposure to UVB. In the presence of thyodene, the blue starch-iodine complex is formed, the intensity of which is proportional to the amount of UVB exposure. Studies were conducted using either a sunlamp or solar ir...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:Photolysis of ester 1 in argon-saturated methanol and acetonitrile does not produce any product, whereas irradiation of 1 in oxygen-saturated methanol yields peroxide 2. Laser flash photolysis studies demonstrate that 1 undergoes intramolecular H atom abstraction to form biradical 3 (λmax ~340 nm), which intersystem c...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.12590
更新日期：2016-05-01 00:00:00
abstract：:Photodynamic therapy (PDT) is an established therapeutic modality that uses nonionizing near-infrared light to activate photocytotoxicity of endogenous or exogenous photosensitizers (PSs). An ongoing avenue of cancer research involves leveraging PDT to stimulate antitumor immune responses; however, these effects appea...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13153
更新日期：2020-03-01 00:00:00
abstract：:The interaction with amino acids of the excited states of the N-oxide resazurin and its deoxygenation product resorufin, has been studied in aqueous solution at pH 7.5. Steady-state and time-resolved studies show that the fluorescence is quenched by amino acids. Complexation of the dyes in the ground state with aromat...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-10-12-RA-342
更新日期：2005-07-01 00:00:00
abstract：:Photodynamic therapy (PDT) appears as a promising alternative in the treatment of breast cancer since it can be highly effective in curing cancer while preserving normal tissue. However, predicting outcomes in PDT still constitutes a great challenge. One of the parameters that are usually empirically determined is the...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/php.13182
更新日期：2020-05-01 00:00:00
abstract：:We report here preliminary pilot study data of the effect of sunless tanning spray with 9% [Correction added after online publication (August 24th, 2009): The concentration of Dihydroxyacetone used in the study was 9% and not 3% as previously stated] dihydroxyacetone (DHA) on 25-hydroxyvitamin D [25(OH)D] serum levels...
journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.2009.00574.x
更新日期：2009-09-01 00:00:00
