Abstract：
:Synthesis of extracellular matrix (ECM) proteins and their degradation by matrix metalloproteinases (MMP) are part of the dermal remodeling resulting from chronic exposure of skin to ultraviolet radiation (UVR). We have compared two alternative mechanisms for these responses, namely, a direct mechanism in which UV-B or UV-A is absorbed by fibroblasts and an indirect mechanism in which cytokines, produced in skin in response to UVR, stimulate production of the ECM proteins and MMP. These studies were carried out on human dermal fibroblasts grown in contracted, free-floating 9 day old collagen gels as a dermal equivalent. Synthesis of tropoelastin, collagen, fibrillin, MMP-1, -2, -3 and -9 and tissue inhibitors of metalloproteinases (TIMP)-1 and -2 were measured. Tropoelastin, collagen and fibrillin levels were stable between days 4 and 10, and MMP and TIMP decreased by day 10. Neither UV-B (2.5-50 mJ/cm2) nor UV-A (2-12 J/cm2) altered synthesis of ECM proteins, but UV-A increased MMP-1 and -3 production. Tropoelastin synthesis increased in response to transforming growth factor-beta1 (5 ng/mL) treatment. Both interleukin-1beta and tumor necrosis factor-alpha (10 ng/mL) decreased fibrillin messenger RNA levels but increased MMP-1, -3 and -9 synthesis markedly. Collagen synthesis was not modulated by UV-B, UV-A or cytokine treatment. These results indicate that certain cytokines may have greater effects on production of ECM proteins and MMP than absorption of UV-B and UV-A by fibroblasts grown in dermal equivalents and suggest that the former pathway may play a role in the dermal remodeling in photoaged skin.
journal_name
Photochem Photobioljournal_title
Photochemistry and photobiologyauthors
Kossodo S,Wong WR,Simon G,Kochevar IEsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
86-93issue
1eissn
0031-8655issn
1751-1097journal_volume
79pub_type
杂志文章abstract：:Chronic exposure of the gray, short-tailed oppossum, Monodelphis domestica to ultraviolet radiation (UVR) induces mesenchymal tumors of the cornea. High molecular weight DNA samples from 6 UVR-induced corneal tumors were assayed for their ability to transform NIH 3T3 cells to tumorigenicity. NIH 3T3 cells transfected ...
journal_title：Photochemistry and photobiology
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doi：10.1111/j.1751-1097.1992.tb04256.x
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doi：10.1111/j.1751-1097.1992.tb04209.x
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doi：10.1111/j.1751-1097.1996.tb01863.x
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journal_title：Photochemistry and photobiology
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journal_title：Photochemistry and photobiology
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更新日期：1990-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
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更新日期：1990-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/php.12833
更新日期：2017-11-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2015-09-01 00:00:00
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doi：10.1111/php.13153
更新日期：2020-03-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05147.x
更新日期：1994-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1751-1097.1994.tb05080.x
更新日期：1994-08-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1562/2004-10-12-RA-342
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更新日期：2018-03-01 00:00:00
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journal_title：Photochemistry and photobiology
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doi：
更新日期：1998-10-01 00:00:00
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更新日期：2000-05-01 00:00:00
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更新日期：2007-07-01 00:00:00
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更新日期：2014-01-01 00:00:00
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更新日期：2009-03-01 00:00:00
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更新日期：2006-11-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1996.tb02421.x
更新日期：1996-07-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：Photochemistry and photobiology
pub_type： 杂志文章
doi：10.1111/j.1751-1097.1992.tb02144.x
更新日期：1992-08-01 00:00:00
