Nanoscale hydroxyl radical generation from multiphoton ionization of tryptophan.

Abstract:

:Exposure of solutions containing both tryptophan and hydrogen peroxide to a pulsed ( approximately 180 fs) laser beam at 750 nm induces luminescence characteristic of 5-hydroxytryptophan. The results indicate that 3-photon excitation of tryptophan results in photoionization within the focal volume of the laser beam. The resulting hydrated electron is scavenged by hydrogen peroxide to produce the hydroxyl radical. The latter subsequently reacts with tryptophan to form 5-hydroxytryptophan. The involvement of hydroxyl radicals is confirmed by the use of ethanol and nitrous oxide as scavengers and their effects on the fluorescence yield in this system. It is postulated that such multiphoton ionization of tryptophanyl residues in cellular proteins may contribute to the photodamage observed during imaging of cells and tissues using multiphoton microscopy.

journal_name

Photochem Photobiol

authors

Bisby RH,Crisostomo AG,Botchway SW,Parker AW

doi

10.1111/j.1751-1097.2008.00447.x

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

353-7

issue

1

eissn

0031-8655

issn

1751-1097

pii

PHP447

journal_volume

85

pub_type

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