The influence of Ala243 (Gly247), Arg215 and Thr226 (Asn230) on the bioluminescence spectra and pH-sensitivity of railroad worm, click beetle and firefly luciferases.

abstract：:Work in the past 8 years, particularly in the past 1-2 years, has greatly expanded our understanding of the mechanisms by which ultraviolet irradiation stimulates melanogenesis in the skin. A direct effect of UV photons on DNA results in up-regulation of the gene for tyrosinase, the rate-limiting enzyme in melanin syn...

journal_title：Photochemistry and photobiology

authors： Gilchrest BA,Park HY,Eller MS,Yaar M

更新日期：1996-01-01 00:00:00

abstract：:The C-40 xanthophylls zeaxanthin and astaxanthin were confirmed to form radical cations, Car.+, in the electron-accepting solvent chloroform by direct excitation using subpicosecond time-resolved absorption spectroscopy in combination with spectroelectrochemical determination of the near-infrared absorption of Car.+. ...

journal_title：Photochemistry and photobiology

authors： Han RM,Tian YX,Wu YS,Wang P,Ai XC,Zhang JP,Skibsted LH

更新日期：2006-03-01 00:00:00

abstract：:Muscle injuries represent ca 30% of sports injuries and excessive stretching of muscle causes more than 90% of injuries. Currently the most used treatments are nonsteroidal anti-inflammatory drugs (NSAIDs), however, in last years, low-level laser therapy (LLLT) is becoming an interesting therapeutic modality. The aim ...

journal_title：Photochemistry and photobiology

authors： de Paiva Carvalho RL,Leal-Junior EC,Petrellis MC,Marcos RL,de Carvalho MH,De Nucci G,Lopes-Martins RA

更新日期：2013-03-01 00:00:00

abstract：:Luciferin-regenerating enzyme (LRE) contributes to in vitro recycling of D-luciferin. In this study, reinvestigation of the luciferase-based LRE assay is reported. Here, using quick change site-directed mutagenesis seven T-LRE (Lampyris turkestanicusLRE) mutants were constructed and the most functional mutant of T-LRE...

journal_title：Photochemistry and photobiology

authors： Hemmati R,Hosseinkhani S,Sajedi RH,Azad T,Tashakor A,Bakhtiari N,Ataei F

更新日期：2015-07-01 00:00:00

abstract：:Photosensitized inhibition of mitochondrial succinoxidase by hypericin was measured in vitro and found to be drug-dose, light-dose, and wavelength dependent. Singlet oxygen generation, monitored using the singlet oxygen trap tetramethylethylene, and oxygen consumption in isolated mitochondria sensitized by hypericin w...

journal_title：Photochemistry and photobiology

authors： Thomas C,MacGill RS,Miller GC,Pardini RS

更新日期：1992-01-01 00:00:00

abstract：:The photophysical properties of Piroxicam, a nonsteroidal anti-inflammatory drug (NSAID), were investigated at different pHext values in reversed micelles of Aerosol-OT (AOT) in iso-octane, using both steady-state and picosecond time-resolved fluorescence spectroscopy. In contrast with the very complex data obtained i...

journal_title：Photochemistry and photobiology

authors： Andrade SM,Costa SM,Pansu R

更新日期：2000-04-01 00:00:00

abstract：:The photodynamic therapy (PDT) activity of the bis(dimethylthexylsiloxy)silicon 2,3-naphthalocyanine (SiNc 8) was evaluated against the EMT-6 tumor implanted intradermally in BALB/c mice. The SiNc 8 was formulated in aqueous emulsions based on Cremophor EL or Solutol HS 15. The formulation was shown to affect plasma c...

journal_title：Photochemistry and photobiology

authors： Brasseur N,Ouellet R,Lewis K,Potter WR,van Lier JE

更新日期：1995-12-01 00:00:00

abstract：:Cumulative and intermittent sun exposure are risk factors for skin cancer, highlighting the need to monitor exposure during childhood. There is currently very little available information concerning the accuracy of self-reported levels of sun exposure, particularly for very young children. In this study, UV radiation ...

journal_title：Photochemistry and photobiology

authors： O'Riordan DL,Stanton WR,Eyeson-Annan M,Gies P,Roy C

更新日期：2000-01-01 00:00:00

abstract：:The growing resistance to antibiotics has rendered antimicrobial photodynamic inactivation (PDI) an attractive alternative treatment modality for infectious diseases. Chitosan (CS) was shown to further potentiate the PDI effect of photosensitizers and was therefore used in this study to investigate its ability to pote...

journal_title：Photochemistry and photobiology

authors： Chen CP,Chen CT,Tsai T

更新日期：2012-05-01 00:00:00

abstract：:The energy and oscillator strength of electronic transitions of chlorophyll (Chl)-amino acid complexes were calculated by using molecular orbital methods. The energies varied widely with coordinated amino acids and the difference between the maximum and minimum energy was about 830 cm-1. This energy difference was com...

journal_title：Photochemistry and photobiology

authors： Nishigaki A,Ohshima S,Nakayama K,Okada M,Nagashima U

更新日期：2001-03-01 00:00:00

abstract：:Ultraviolet radiation can inhibit immune responses locally as well as systemically. Such effects have been measured in animals and humans exposed to ultraviolet B (wavelength 280-315 nm) (UVB) and ultraviolet A (315-400 nm) (UVA). The precise wavelength dependence is important for the identification of possible molecu...

journal_title：Photochemistry and photobiology

authors： Garssen J,de Gruijl F,Mol D,de Klerk A,Roholl P,Van Loveren H

更新日期：2001-04-01 00:00:00

abstract：:There are controversial reports in the literature concerning the reactivity of singlet oxygen ((1)O(2)) with the redox probe 2',7'-dichlorodihydrofluorescein (DCFH). By carefully preparing solutions in which (1)O(2) is quantitatively generated in the presence of DCFH, we were able to show that the formation rate of th...

journal_title：Photochemistry and photobiology

authors： Daghastanli NA,Itri R,Baptista MS

更新日期：2008-09-01 00:00:00

abstract：:Microscopic fluorescence photometry incorporating a light-sensitive thermo-electrically cooled charge-coupled device (CCD) camera was employed to investigate the fluorescence distribution of 5-aminolevulinic acid (ALA)-induced porphyrins in 22 patients with a total number of 52 noduloulcerative basal cell carcinomas (...

journal_title：Photochemistry and photobiology

authors： Peng Q,Warloe T,Moan J,Heyerdahl H,Steen HB,Nesland JM,Giercksky KE

更新日期：1995-11-01 00:00:00

abstract：:Singlet oxygen reacts preferentially with three amino acids in proteins, His, Trp and Met. In order to study the specific molecular events that result from such oxidations, calf alpha-crystallin was photooxidized in the presence of uroporphyrin and the reactions were investigated by high performance liquid chromatogra...

journal_title：Photochemistry and photobiology

authors： Dillon J,Chiesa R,Wang RH,McDermott M

更新日期：1993-03-01 00:00:00

abstract：:Photodynamic therapy (PDT) appears as a promising alternative in the treatment of breast cancer since it can be highly effective in curing cancer while preserving normal tissue. However, predicting outcomes in PDT still constitutes a great challenge. One of the parameters that are usually empirically determined is the...

journal_title：Photochemistry and photobiology

authors： Dos Santos AF,de Almeida DRQ,Terra LF,Wailemann RAM,Gomes VM,Arini GS,Ravagnani FG,Baptista MS,Labriola L

更新日期：2020-05-01 00:00:00

abstract：:Herpes simplex virus (HSV) normally causes vescular lesions on mucocutaneous surfaces but can also cause encephalitis. The virus can reactivate from the latent state in neurons to form recrudescent lesions. One common stimulus for reactivation is exposure to sunlight. In the present study, the effects of irradiating r...

journal_title：Photochemistry and photobiology

authors： Garssen J,van der Molen R,de Klerk A,Norval M,van Loveren H

更新日期：2000-11-01 00:00:00

abstract：:We developed a novel method for the isolation of the PSI-LHCI-LHCII complex from spinach leaves. The supercomplex was resolved into a core complex (CPI), LHCII trimers, LHCI dimers and LHCII monomers using green gel electrophoresis. We then investigate changes in the fluorescence and absorption spectra of PSI-LHCI-LHC...

journal_title：Photochemistry and photobiology

authors： Qin X,Wang W,Wang K,Xin Y,Kuang T

更新日期：2011-01-01 00:00:00

abstract：:All-trans retinoic acid (ATRA) plays essential roles in the normal biological processes and the treatment of cancer and skin diseases. Considering its photosensitive property, many studies have been focused on the photochemistry of ATRA. In this study, we investigated the transient phenomena in the laser flash photoly...

journal_title：Photochemistry and photobiology

authors： Li K,Wang M,Wang T,Sun D,Zhu R,Sun X,Wu X,Wang SL

更新日期：2013-09-01 00:00:00

abstract：:Efficacy of ionizing radiation (I/R) was compared with phototoxic effects of photodynamic therapy (PDT) in vitro using two cell lines derived from patients with head and neck squamous cell carcinoma (HNSCC). A cell line derived from a donor with a human papilloma virus (HPV) infection was more responsive to I/R but si...

journal_title：Photochemistry and photobiology

authors： Kessel D,Cho WJ,Rakowski J,Kim HE,Kim HC

更新日期：2020-05-01 00:00:00

abstract：:A structural subunit (called B816) has been isolated from the B875 light-harvesting complex of Rhodobacter capsulatus using a detergent-mediated dissociation of chromatophores. Rb. capsulatus MW442 (B800-850- B875+ car+) chromatophores were extracted with benzene and titrated with octyl glucoside (OG) to shift the nea...

journal_title：Photochemistry and photobiology

authors： Heller BA,Loach PA

更新日期：1990-05-01 00:00:00

abstract：:More scheduled outdoor stay is increasingly advocated for school children. This study measured 2(nd) , 5(th) and 8(th) graders' erythemal UV-exposure in September, March and May at four Swedish schools. We related those exposures, as fractions of total available ambient radiation, to the schools outdoor environments d...

journal_title：Photochemistry and photobiology

authors： Pagels P,Wester U,Söderström M,Lindelöf B,Boldemann C

更新日期：2016-01-01 00:00:00

abstract：:A diffusible multitherapy resistance factor (MTRF) is produced by Cloudman S91 melanoma cells in vitro. The MTRF decreases sensitivity of the target cell line, S91/amel, to gamma-irradiation, UVC (200-280 nm) and mitomycin C (MMC). In the present study, we demonstrate that MTRF also increases the survival of S91/amel ...

journal_title：Photochemistry and photobiology

authors： Hill HZ,Hill GJ,Cieszka K,Azure M,Chowdhary I,Sayre RM

更新日期：1995-05-01 00:00:00

abstract：:Photoreduction of cytochrome c (Cyt c) has been reinvestigated using femtosecond-to-nanosecond transient absorption and stationary spectroscopy. Femtosecond spectra of oxidized Cyt c, recorded in the probe range 270-1000 nm, demonstrate similar evolution upon 266 or 403 nm excitation: an ultrafast 0.3 ps internal conv...

journal_title：Photochemistry and photobiology

authors： Löwenich D,Kleinermanns K,Karunakaran V,Kovalenko SA

更新日期：2008-01-01 00:00:00

abstract：:Vibrio fischeri strain Y1 emits yellow light in vivo due to the participation of a yellow fluorescent protein (YFP) in the luciferase reaction. In this study it was found that the organism also produces a protein (referred to as Y1-BFP) emitting strong blue fluorescence. Its molecular weight, about 25 kDa, is the same...

journal_title：Photochemistry and photobiology

authors： Karatani H,Wilson T,Hastings JW

更新日期：1992-02-01 00:00:00

abstract：:The bimolecular rate constants (k) of quenching of molecular singlet oxygen 1O2 (1 delta g) by organo-selenium compounds exhibiting glutathione peroxidase activity and by sulfur analogs have been determined by time resolved phosphorescence detection of 1O2 in CD3OD and C6D6, with no solvent effect. The rate constants ...

journal_title：Photochemistry and photobiology

authors： Scurlock R,Rougée M,Bensasson RV,Evers M,Dereu N

更新日期：1991-11-01 00:00:00

abstract：:In this manuscript, we demonstrate the control of film thickness and surface wettability in the photochemical immobilization of poly (vinyl alcohol) (PVA) to a self-assembled monolayer (SAM) containing a phthalimide chromophore. Surface attachment is characterized by ellipsometry and contact angle measurements. The we...

journal_title：Photochemistry and photobiology

authors： Carroll GT,Turro NJ,Mammana A,Koberstein JT

更新日期：2017-10-01 00:00:00

abstract：:In the present study, hypericin analogs with an increased hydrophilic character were synthesized. As chemical modifications alter the lipophilicity/hydrophilicity balance together with the photophysical/chemical background of the molecule the influence of these structural changes on the cellular uptake, retention and ...

journal_title：Photochemistry and photobiology

authors： Delaey EM,Obermuëller R,Zupkó I,De Vos D,Falk H,de Witte PA

更新日期：2001-08-01 00:00:00

abstract：:To improve the understanding of human skin autofluorescence emission, the spectroscopic and microscopic characteristics of skin autofluorescence were studied using a combined fluorescence and reflectance spectroanalyzer and a fiber optic microspectrophotometer. The autofluorescence spectra of in vivo human skin were m...

journal_title：Photochemistry and photobiology

authors： Zeng H,MacAulay C,McLean DI,Palcic B

更新日期：1995-06-01 00:00:00

abstract：:We have demonstrated that it is possible to launch UVC LED light into bacterial contaminated polymer tubes/catheters and disinfect the intraluminal space of these tubes. This can be achieved by UVC treatment of the catheters on a regular basis. Catheters are in the distal end equipped with an exit hole for administrat...

journal_title：Photochemistry and photobiology

authors： Bak J,Jørgensen TM,Helfmann J,Gravemann U,Vorontsova I

更新日期：2011-03-01 00:00:00

abstract：:A novel antibody-fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N-terminal bound, fluorescently labeled Oat-25 Fab antibody fragments to the phytochrome chromophore was measured. The results sugge...

journal_title：Photochemistry and photobiology

authors： Farrens DL,Cordonnier MM,Pratt LH,Song PS

更新日期：1992-11-01 00:00:00