当前位置： SCI文献检索 > PHOTOCHEMISTRY AND PHOTOBIOLOGY期刊下所有文献 > Encapsulation of p-THPP into nanoparticles: cellular uptake, subcellular localization and effect of serum on photodynamic activity.

Encapsulation of p-THPP into nanoparticles: cellular uptake, subcellular localization and effect of serum on photodynamic activity.

Abstract：

:The cellular uptake, localization and efflux of meso-tetra-(4-hydroxyphenyl)porphyrin (p-THPP)-loaded nanoparticles have been studied in EMT-6 tumor cells. The effect of blood serum on photocytotoxicity has also been evaluated. Sub-130 nm nanoparticles based on poly(D,L-lactide-co-glycolide) (PLGA) (50:50 PLGA and 75:25 PLGA) and poly(D,L-lactide) (PLA) have been examined in comparison with free p-THPP. For all formulations tested, uptake of photosensitizer into cells was dependent on concentration, time and temperature. All nanoparticulate formulations accumulated within the cells to a greater extent relative to free drug. Indeed, the fluorescence intensities measured on EMT-6 cells treated with p-THPP-loaded nanoparticulate formulations were at least two-fold higher than those obtained with free dye. Furthermore, the highest accumulation level was found with PLGA nanoparticles. Fluorescence microscopy revealed that endocytosis is a major intracellular sequestration mechanism of these p-THPP formulations and that these were localized into early and late endosomes. The efflux study performed on both nonirradiated and irradiated cells indicated that free and p-THPP-loaded nanoparticles gradually escaped from EMT-6 cells as a function of time. This was more pronounced when cells were treated with nanoparticles and irradiated, reflecting important photodamage. It was also found that regardless of the nanoparticulate formulations tested, p-THPP photocytotoxicity was influenced by the concentration of the serum.

journal_name

Photochem Photobiol

journal_title

Photochemistry and photobiology

authors

Konan YN,Chevallier J,Gurny R,Allémann E

doi

10.1562/0031-8655(2003)077<0638:eopinc>2.0.co;2

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

638-44

issue

6

eissn

0031-8655

issn

1751-1097

journal_volume

77

pub_type

杂志文章

相关文献

PHOTOCHEMISTRY AND PHOTOBIOLOGY文献大全
  • Retarded photooxidation of cyamemazine in biomimetic microenvironments.

    abstract：:Cyamemazine (CMZ) is a neuroleptic drug that mediates cutaneous phototoxicity in humans. Here, the photobehavior of CMZ has been examined within α1 -acid glycoproteins, β- and γ-cyclodextrins and SDS micelles. In all these microenvironments, CMZ emission was enhanced and blue-shifted, and its lifetime was longer. Irra...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12303

    authors： Limones-Herrero D,Pérez-Ruiz R,Jiménez MC,Miranda MA

    更新日期：2014-09-01 00:00:00

  • Resonance Raman spectra of bacteriorhodopsin mutants with substitutions at Asp-85, Asp-96, and Arg-82.

    abstract：:Detergent solubilized bacteriorhodopsin (BR) proteins which contain alterations made by site-directed mutagenesis (Asp-96----Asn, D96N; Asp-85----Asn, D85N; and Arg-82----Gln, R82Q) have been studied with resonance Raman spectroscopy. Raman spectra of the light-adapted (BRLA) and M species in D96N are identical to tho...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1991.tb03638.x

    authors： Lin SW,Fodor SP,Miercke LJ,Shand RF,Betlach MC,Stroud RM,Mathies RA

    更新日期：1991-03-01 00:00:00

  • Analysis of two kinds of tree as physical barriers against erythemal UVB radiation received.

    abstract：:Differences between global radiation UVER (erythemal ultraviolet solar radiation) received under full sun and diffuse radiation received under the shadow of two types of tree are analyzed to check the importance of these components on human exposure to UV radiation. Blue Line spores dosimeters of VioSpor were used for...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12020

    authors： Ysasi GG,Ribera LJ

    更新日期：2013-05-01 00:00:00

  • Novel Collimated Beam Setup to Study the Kinetics of VUV-Induced Reactions.

    abstract：:Vacuum UV (VUV) process is an incipient advanced oxidation process, which can be used for water treatment. This process relies on the formation of hydroxyl radicals through the VUV-induced photolysis of water. In particular, the use of ozone-generating mercury vapor lamps, which emit 10% of the radiation at 185 nm and...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12157

    authors： Duca C,Imoberdorf G,Mohseni M

    更新日期：2014-01-01 00:00:00

  • Chaetopterus variopedatus Bioluminescence: A Review of Light Emission within a Species Complex.

    abstract：:Chaetopterus variopedatus has been studied for over a century in terms of its physiology, ecology and life history. One focus of research is on its intrinsic bioluminescent emissions, which can be observed as a blue light emitted from the extremities of individual body segments, or as a secreted mucus. Even though res...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章,评审

    doi：10.1111/php.13221

    authors： Mirza JD,Migotto ÁE,Yampolsky IV,de Moraes GV,Tsarkova AS,Oliveira AG

    更新日期：2020-07-01 00:00:00

  • Applicability of the polysulphone horizontal calibration to differently inclined dosimeters.

    abstract：:Polysulphone (PS) dosimetry has been a widely used technique for more than 30 years to quantify the erythemally effective UV dose received by anatomic sites (personal exposure). The calibration of PS dosimeters is an important issue as their spectral response is different from the erythemal action spectrum. It is perf...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2011.01006.x

    authors： Casale GR,Siani AM,Diémoz H,Kimlin MG,Colosimo A

    更新日期：2012-01-01 00:00:00

  • Synergistic effect of ultrasound and thiazone-PEG 400 on human skin optical clearing in vivo.

    abstract：:In this paper, we propose a new physical method in combination with mixed solution of thiazone and polyethylene glycol 400 (thiazone PEG 400 solution) penetration into tissue to assess the skin optical clearing. Four treatments were performed: (1) control group (C); (2) polyethylene glycol 400 (PEG400); (3) 0.25% thia...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2010.00710.x

    authors： Zhong H,Guo Z,Wei H,Guo L,Wang C,He Y,Xiong H,Liu S

    更新日期：2010-05-01 00:00:00

  • Photophysical properties of tin ethyl etiopurpurin I (SnET2) and tin octaethylbenzochlorin (SnOEBC) in solution and bound to albumin.

    abstract：:The photophysical characteristics of two second-generation PDT photosensitizers, tin ethyl etiopurpurin I (SnET2) and tin octaethylbenzochlorin (SnOEBC), have been measured in homogeneous solution and when bound to bovine serum albumin (BSA). The ground state and triplet state absorption spectra have been characterize...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：

    authors： Pogue BW,Redmond RW,Trivedi N,Hasan T

    更新日期：1998-12-01 00:00:00

  • Promotion of photodynamic therapy-induced apoptosis by the mitochondrial protein Smac/DIABLO: dependence on Bax.

    abstract：:Photodynamic therapy (PDT) using the second-generation photosensitizer phthalocyanine (Pc) 4 causes mitochondrial damage and induces apoptosis through the release of cytochrome c to the cytosol. Another protein of the mitochondrial intermembrane space, Smac/DIABLO (second mitochondria-derived activator of caspase/dire...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2002)076<0217:poptia>2.0.co;2

    authors： Usuda J,Chiu SM,Azizuddin K,Xue LY,Lam M,Nieminen AL,Oleinick NL

    更新日期：2002-08-01 00:00:00

  • Application of CdTe/ZnSe quantum dots in in vitro imaging of chicken tissue and embryo.

    abstract：:The present work is aimed to synthesize CdTe/ZnSe core/shell quantum dots (QDs) in an easy way and to explore the possibilities of its application in in vitro imaging of chicken tissue and embryo. The QDs were prepared using microwave irradiation with different temperatures, which is a very easy and less time-consumin...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12398

    authors： Moulick A,Blazkova I,Milosavljevic V,Fohlerova Z,Hubalek J,Kopel P,Vaculovicova M,Adam V,Kizek R

    更新日期：2015-03-01 00:00:00

  • Cyclobutane dimers and (6-4) photoproducts in human cells are mended with the same patch sizes.

    abstract：:The size of excision repair patches corresponding to excision of (6-4) pyrimidine-pyrimidone photoproducts and (5-5, 6-6) cyclobutane dimers have been independently determined by using bromodeoxyuridine substitution and density increases in isopycnic gradients of small DNA fragments. The two classes of photoproducts w...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1991.tb02033.x

    authors： Cleaver JE,Jen J,Charles WC,Mitchell DL

    更新日期：1991-09-01 00:00:00

  • Enhanced response to daunomycin of normal, tumor and metastatic cell lines via drug photoactivation.

    abstract：:We have compared the cytotoxicity of daunomycin in vitro to highly differentiated normal epithelial cells (Fisher rat thyroid cells, FRTL-5) and to two neoplastic cell lines, a thyroid carcinoma (TK-6) and its lung metastasis (MPTK-6). Whereas the cell lines are equally sensitive to the drug in the dark, if irradiated...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.1993.tb09223.x

    authors： Andreoni A,Colasanti A,Kisslinger A,Mastrocinque M,Portella G,Riccio P,Roberti G

    更新日期：1993-05-01 00:00:00

  • Functional insights gained from structural analyses of DNA duplexes that contain UV-damaged photoproducts.

    abstract：:Ultraviolet photolesions endow DNA with distinct structural and dynamic properties. Biophysical studies of photoproduct-containing DNA have shown that these lesions affect the mutagenic properties of DNA and damage recognition by DNA repair systems. Recently obtained high-resolution cocrystal structures of damaged DNA...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章,评审

    doi：10.1562/2006-02-28-IR-820

    authors： Park CJ,Lee JH,Choi BS

    更新日期：2007-01-01 00:00:00

  • A Sharp Jump in Photocatalytic Activity of Elemental Sulfur for Dye Degradation in Alkaline Solution.

    abstract：:Elemental sulfur is a low-cost and abundant substance as one of the largest by-products of the oil industry which was widely used in many industrial activities. Cyclo-octasulfur (S8 ) is one of the sulfur allotropes that is a very stable substance in standard conditions. In this study, we report a low-cost and fast me...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.13301

    authors： Peyman VA,Azizian S

    更新日期：2020-11-01 00:00:00

  • The Anthocyanins, Oenin and Callistephin, Protect RPE Cells Against Oxidative Stress.

    abstract：:The retinal pigment epithelium (RPE) is a highly metabolic layer of postmitotic cells lining Bruch's membrane in the retina. While these cells contain endogenous photosensitizers that mediate blue light-induced damage, it has also been shown that blue light exposure damages mitochondrial DNA in RPE cells resulting in ...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12683

    authors： Yacout SM,Gaillard ER

    更新日期：2017-03-01 00:00:00

  • Biodistribution and pharmacokinetic studies of a porphyrin dimer photosensitizer (Oxdime) by fluorescence imaging and spectroscopy in mice bearing xenograft tumors.

    abstract：:Herein, we present a study of the pharmacokinetics and biodistribution of a butadiyne-linked conjugated porphyrin dimer (Oxdime) designed to have high near-infrared (NIR) 2-photon absorption cross-section for photodynamic therapy (PDT). Changes in biodistribution over time were monitored in mice carrying B16-F10 melan...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2012.01194.x

    authors： Khurana M,Ulrich S,Kim A,Moriyama Y,Netchev G,Akens MK,Anderson HL,Wilson BC

    更新日期：2012-11-01 00:00:00

  • Application of molecular orbital calculations to interpret the chlorophyll spectral forms in pea photosystem II.

    abstract：:The energy and oscillator strength of electronic transitions of chlorophyll (Chl)-amino acid complexes were calculated by using molecular orbital methods. The energies varied widely with coordinated amino acids and the difference between the maximum and minimum energy was about 830 cm-1. This energy difference was com...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2001)073<0245:aomoct>2.0.co;2

    authors： Nishigaki A,Ohshima S,Nakayama K,Okada M,Nagashima U

    更新日期：2001-03-01 00:00:00

  • Effects of N-Glycosylation Deletions on Cypridina Luciferase Activity.

    abstract：:Cypridina luciferase (Cluc), a secreted luminescent protein identified from Cypridina noctiluca, has two N-glycosylation sites. In this study, we evaluated the effects of N-glycosylation on Cluc properties by creating site-directed mutagenic modifications at the consensus sequence for N-glycosylation (Asn-X-Ser/Thr). ...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12847

    authors： Yasuno R,Mitani Y,Ohmiya Y

    更新日期：2018-03-01 00:00:00

  • Photophysics and photochemistry of z-chlorprothixene in acetonitrile.

    abstract：:Chlorprothixene (CPTX, Taractan) is a low potency antipsychotic mainly used for the treatment of psychotic disorders (e.g. schizophrenia) and acute mania occurring as part of bipolar disorders. As in the case of other numerous drugs used in the treatment of psychiatric disorders, CPTX presents geometric isomerism. The...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2009.00584.x

    authors： Piñero LE,García C,Lhiaubet-Vallet V,Oyola R,Miranda MA

    更新日期：2009-07-01 00:00:00

  • Photochemical and Photodynamical Properties of Sulfur-Substituted Nucleic Acid Bases.

    abstract：:Sulfur-substituted nucleobases (a.k.a., thiobases) are among the world's leading prescriptions for chemotherapy and immunosuppression. Long-term treatment with azathioprine, 6-mercaptopurine and 6-thioguanine has been correlated with the photoinduced formation of carcinomas. Establishing an in-depth understanding of t...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章,评审

    doi：10.1111/php.12975

    authors： Ashwood B,Pollum M,Crespo-Hernández CE

    更新日期：2019-01-01 00:00:00

  • Type I and Type II Photosensitized Oxidation Reactions: Guidelines and Mechanistic Pathways.

    abstract：:Here, 10 guidelines are presented for a standardized definition of type I and type II photosensitized oxidation reactions. Because of varied notions of reactions mediated by photosensitizers, a checklist of recommendations is provided for their definitions. Type I and type II photoreactions are oxygen-dependent and in...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章,评审

    doi：10.1111/php.12716

    authors： Baptista MS,Cadet J,Di Mascio P,Ghogare AA,Greer A,Hamblin MR,Lorente C,Nunez SC,Ribeiro MS,Thomas AH,Vignoni M,Yoshimura TM

    更新日期：2017-07-01 00:00:00

  • Cooperative phenomena in two-pulse, two-color laser photocoagulation of cutaneous blood vessels.

    abstract：:A novel laser system has been developed to study the effects of multiple laser pulses of differing wavelengths on cutaneous blood vessels in vivo, using the hamster dorsal skin flap preparation and in vitro, using cuvettes of whole or diluted blood. The system permits sequenced irradiation with well-defined intrapulse...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/0031-8655(2001)073<0642:cpitpt>2.0.co;2

    authors： Barton JK,Frangineas G,Pummer H,Black JF

    更新日期：2001-06-01 00:00:00

  • Scrambled self-assembly of bacteriochlorophylls c and e in aqueous Triton X-100 micelles.

    abstract：:Bacteriochlorophyll (BChl) e was coassembled with BChl c in Triton X-100 micelles in aqueous solutions. The Qy absorption bands of the coaggregates were positioned between those of aggregates consisting solely of BChl c or e. The electronic absorption spectra of the coaggregates could not be reproduced by linear combi...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12219

    authors： Saga Y,Saiki T,Takahashi N,Shibata Y,Tamiaki H

    更新日期：2014-05-01 00:00:00

  • Fluence Rate Determines PDT Efficiency in Breast Cancer Cells Displaying Different GSH Levels.

    abstract：:Photodynamic therapy (PDT) appears as a promising alternative in the treatment of breast cancer since it can be highly effective in curing cancer while preserving normal tissue. However, predicting outcomes in PDT still constitutes a great challenge. One of the parameters that are usually empirically determined is the...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.13182

    authors： Dos Santos AF,de Almeida DRQ,Terra LF,Wailemann RAM,Gomes VM,Arini GS,Ravagnani FG,Baptista MS,Labriola L

    更新日期：2020-05-01 00:00:00

  • Effectiveness of Photobiomodulation as an Adjunct to Nonsurgical Periodontal Therapy in the Management of Periodontitis- A Systematic Review of in vivo Human Studies.

    abstract：:Photobiomodulation therapy (PBMT) has anti-inflammatory, analgesic and regenerative properties. This systematic review aimed to critically appraise the published data of in vivo human randomized clinical trials (RCTs), and present a comprehensive overview of the efficacy of PBMT, as an adjunct to the nonsurgical perio...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章,评审

    doi：10.1111/php.13348

    authors： Dalvi S,Benedicenti S,Hanna R

    更新日期：2020-10-24 00:00:00

  • Phototaxis of Haloarcula marismortui revealed through a novel microbial motion analysis algorithm.

    abstract：:Haloarcula marismortui has been described to be nonmotile prior to the recent identification of flagellar filaments, suggesting the motile nature of H. marismortui. Here we observed the locomotion of freshly cultured H. marismortui cells and tracked the swimming trajectories via ImageJ. Trajectories of H. marismortui ...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2010.00768.x

    authors： Lin YC,Fu HY,Yang CS

    更新日期：2010-09-01 00:00:00

  • Physicochemical studies of demetalation of light-harvesting bacteriochlorophyll isomers purified from green sulfur photosynthetic bacteria.

    abstract：:Demetalation kinetics of bacteriochlorophylls (BChls) c, d and e from green sulfur photosynthetic bacteria were studied under weakly acidic conditions. Demetalation rate constants of BChl e possessing a formyl group at the 7-position were significantly smaller than those of BChls c and d, which had a methyl group at t...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/j.1751-1097.2009.00580.x

    authors： Hirai Y,Tamiaki H,Kashimura S,Saga Y

    更新日期：2009-09-01 00:00:00

  • Estimating the Diurnal Cycle and Daily Insolation of Ultraviolet and Photosynthetically Active Radiation at the Sea Surface.

    abstract：:Accurate determination of the diurnal variability and daily insolation of surface (0(+) ) and subsurface (0(-) ) irradiance are essential to estimate several physical, chemical and biological processes occurring at the surface layer of marine environments. Natural downwelling PAR and spectral UVR were examined on eigh...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.12474

    authors： Kuwahara VS,Taguchi S

    更新日期：2015-09-01 00:00:00

  • Ultraviolet B but not A radiation activates suppressor B cells in draining lymph nodes.

    abstract：:Immunosuppressive doses of solar-simulated UV radiation activate lymph node B cells that can suppress primary immunity by inhibiting the function of dendritic cells. The aim of this study was to determine the waveband responsible for activation of these suppressor B cells. We exposed C57BL/6 mice to various doses of e...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1562/2005-04-20-RA-495

    authors： Byrne SN,Ahmed J,Halliday GM

    更新日期：2005-11-01 00:00:00

  • Asiatic Acid Glucosamine Salt Alleviates Ultraviolet B-induced Photoaging of Human Dermal Fibroblasts and Nude Mouse Skin.

    abstract：:Herbal extracts including asiatic acid (AA) have become popular candidates of anti-photoaging agents due to their anti-inflammatory and antioxidant properties and minimal side effect. Nevertheless, low bioavailability due to poor solubility limits their practical application. In this study, a highly bioavailable form ...

    journal_title：Photochemistry and photobiology

    pub_type： 杂志文章

    doi：10.1111/php.13160

    authors： Huang J,Tu T,Wang W,Zhou G,Zhang W,Wu X,Liu W

    更新日期：2020-01-01 00:00:00