Abstract:
:RNase-sensitive DNA polymerase activity (RSDP) was tested in different cell fractions of Neurospora crassa cell types and its morphological mutants. This RSDP was found localized in the microsomal pellet fraction and absent in the purified nuclear pellets isolated from different N. crassa cell types: conidia, germinated conidia, and mycelia. This enzyme is capable of synthesizing a DNA product only in the presence of all four deoxyribonucleoside-5' - triphosphates and Mg2+. Removal of RNA from the pellet fraction by RNase strongly inhibited the DNA synthesis. The endogenous synthesis of DNA in the microsomal pellet fraction was associated with the formation of an RNA:DNA hybrid as analyzed by Cs2SO4 equilibrium density gradient centrifugation. The DNA product after alkali hydrolysis hybridizes with the RNA isolated from the same pellet fraction, as analyzed by elution from hydroxylapatite column at 60C. This DNa product did not hybridize with poly (A). A few mutants tested showed this RNase-sensitive DNA polymerase activity.
journal_name
Biochem Genetjournal_title
Biochemical geneticsauthors
Dutta SK,Mukhopadhyay DK,Bhattachryya Jdoi
10.1007/BF00484590subject
Has Abstractpub_date
1980-08-01 00:00:00pages
743-53issue
7-8eissn
0006-2928issn
1573-4927journal_volume
18pub_type
杂志文章abstract::The stem-loop potential of a nucleic acid segment (expressed as a FONS value), decomposes into base composition-dependent and base order-dependent components. The latter, expressed as a FORS-D value, is derived by subtracting the value of the base composition-dependent component (FORS-M) from the FONS value. FORS-D an...
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更新日期:1996-10-01 00:00:00
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更新日期:1998-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
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更新日期:1981-06-01 00:00:00
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journal_title:Biochemical genetics
pub_type: 杂志文章
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journal_title:Biochemical genetics
pub_type: 杂志文章
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更新日期:1978-04-01 00:00:00