Abstract：
:The density of bovine peripheral-type benzodiazepine receptors (PBR) in four tissues was highest in adrenal cortex. The adrenal cortex PBR cofractionated with a mitochondrial membrane marker enzyme and could be solubilized with intact ligand binding properties using digitonin. The membrane bound and soluble mitochondrial receptors were pharmacologically characterized and showed the rank order of potency to inhibit [3H]PK 11195 binding was PK 11195 greater than protoporphyrin IX greater than benzodiazepines (clonazepam, diazepam, or Ro5-4864). [3H]PK 11195 binding to bovine adrenal mitochondria was unaffected by diethylpyrocarbonate, a histidine residue modifying reagent that decreased binding to rat liver mitochondria by 70%. [3H]PK 14105 photolabeled the bovine PBR and the Mr was estimated under nondenaturing (200 kDa) and denaturing (17 kDa) conditions. These results demonstrate the bovine peripheral-type benzodiazepine receptor is pharmacologically and biochemically distinct from the rat receptor, but the receptor component photolabeled by an isoquinoline ligand has a similar molecular weight.
journal_name
Life Scijournal_title
Life sciencesauthors
Parola AL,Laird HE 2nddoi
10.1016/0024-3205(91)90090-xsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
757-64issue
8eissn
0024-3205issn
1879-0631pii
0024-3205(91)90090-Xjournal_volume
48pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Immunotherapy methods using potential tumor microenvironment modulators have elicited durable therapeutic responses in cancer treatment. Immune checkpoint molecule programmed cell death-ligand 1 (PD-L1) and oncogenic transcription factor STAT3 (signal transducer and activator of transcription-3) assigned as inhibitory...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118847
更新日期：2021-02-01 00:00:00
abstract：AIMS:Dorsal root ganglia contain heterogeneous populations of primary afferent neurons that transmit various sensory stimuli. This functional diversity may be correlated with differential expression of voltage-gated K(+) (Kv) channels. Here, we examine cellular distributions of Kv4 pore-forming and ancillary subunits t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.07.007
更新日期：2012-09-17 00:00:00
abstract：:This study examined the protective effect of phenobarbital on kainic acid-induced deficits in acquisition learning. A single kainic acid injection (9 mg/kg i.p.) was administered five days prior to testing using the Morris water maze test. Kainic acid produced deficits in the acquisition of spatial information observe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00658-5
更新日期：2000-06-30 00:00:00
abstract：:Pitavastatin is a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor that is used for suppressing cholesterol biosynthesis. Previously, we have reported that pitavastatin induces the activation of endothelial nitric oxide synthase (eNOS) and increases nitric oxide (NO) production in vascular endothelial cells (...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.12.003
更新日期：2005-03-25 00:00:00
abstract：:Sexual interactions have multiple effects on oxytocin systems in the brain. In the present study we observed that allowing estrogen-progesterone treated ovariectomized rats to be mounted ten times by a male significantly decreased the density (beta max) of thymic oxytocin receptors. Animals were ovariectomized and aft...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90467-h
更新日期：1993-01-01 00:00:00
abstract：:Synthetic human pancreatic Growth Hormone-Releasing Factor (hpGRF) elevated the plasma concentration of growth hormone (GH) in young and adult domestic fowl. This in vivo effect of hpGRF appeared to be largely similar for both the 32 amino-acid (hpGRF 1-32) or 40 amino-acid (hpGRF 1-40) polypeptide, although the effec...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90083-3
更新日期：1984-03-19 00:00:00
abstract：:Two-dimensional and line-scan analyses of the early phase Ca2+ transients in rat cardiomyocytes were performed with a rapid-scanning laser confocal microscope and fluo-3 to elucidate the mechanism of activation of Ca2+ release from the sarcoplasmic reticulum in atrial myocytes which lack a well developed T-tubular net...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01436-9
更新日期：2001-12-28 00:00:00
abstract：:The epicardial release of immunoreactive atrial natriuretic peptides (ir-ANPs) in inside-out perfused rabbit atria has been reported. In order to determine the presence of ir-ANPs in pericardial fluid and to evaluate their biochemical characteristics, we measured the concentration of ir-ANPs in pericardial fluid obtai...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90514-r
更新日期：1990-01-01 00:00:00
abstract：AIMS:Resveratrol (Res) which is a polyphenolic phytoalexin, has various biological properties. In the present study, we investigated whether Res extracted from Polygonum cuspidatum can reduce oxidative damage and cognitive impairment in senescence-accelerated mouse (SAM). MAIN METHODS:Senescence-accelerated mice were ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.08.033
更新日期：2012-10-29 00:00:00
abstract：:Arsenic has been proposed as a chemotherapeutic agent for leukemia and other solid tumors. However, its environmental exposure has been linked epidemiologically with an elevated carcinoma risk (i.e. skin, bladder and lung), with cellular oxidative stress being implicated in both induced-arsenic toxicity and carcinogen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.09.008
更新日期：2007-10-13 00:00:00
abstract：:TNF production has been studied in peritoneal macrophages and splenic T cells of Arctic Yakutian ground squirrel (Citellus Undulatus Pallas) in hibernating and awake animals in winter and in prehibernating autumn as well as in active euthermic spring-summer animals. A high level of TNF production in macrophages of gro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00698-6
更新日期：2000-01-01 00:00:00
abstract：:Dopamine receptors in human parathyroid were studied in vitro using ligand binding techniques. With 3H-piflutixol as ligand, binding characteristic of the dopamine D1 receptor was observed. Administration of apomorphine, flupenthixol or metoclopramide to normal controls or acute schizophrenic patients at doses produci...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90779-8
更新日期：1983-08-22 00:00:00
abstract：:These experiments were designed to test the hypothesis that cyclosporine A (CSA) inhibits renin secretion and stimulates renal prostaglandin E2 (PGE2) release in vitro. In rat renal cortical slices incubated at 37 degrees C in a buffered and oxygenated physiological saline solution containing 4 mM KCl, CSA concentrati...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90187-v
更新日期：1990-01-01 00:00:00
abstract：:Alpha adrenergic agonists and antagonists as clonidine, guanfacine, yohimbine, phenylephrine and prazosin inhibited the [3H]-QNB binding to rat brain cortex muscarinic acetylcholine receptor (mAChR, M-1 subtype), heart (M-2 subtype) and parotid gland homogenate (M-3 subtype) in a dose-dependent competitive fashion. Ki...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90436-u
更新日期：1990-01-01 00:00:00
abstract：:P-glycoprotein (P-gp) is found to play a very significant role in intestinal and biliary transport of irinotecan and its active metabolite, SN-38. This makes P-gp inhibition a logical strategy for improving irinotecan's oral efficacy and reducing its toxicity. The objective of the present study was to identify the mos...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.06.007
更新日期：2008-08-15 00:00:00
abstract：:Alpha-inhibin-92 (alpha-IB-92) has been characterized from human seminal plasma and found to be active in suppressing FSH release in vitro and in vivo. In order to determine if smaller fragments of this 92 amino acid peptide would still be active to suppress FSH release, we have evaluated 5 of these fragments for thei...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90100-7
更新日期：1994-01-01 00:00:00
abstract：:We explored the mechanism whereby maltitol causes an increase of calcium absorption in the lower small intestine using everted ileal segments of rats. Under the calcium concentrations tested (1-20mM), which enabled us to assess the diffusional calcium transfer, maltitol in the mucosal-side medium (100mM) caused a 155%...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00430-4
更新日期：1996-01-01 00:00:00
abstract：:Both in men and rats, most of the ethanol ingested at a low dose is metabolized before it reaches the systemic circulation. Oxidation of ethanol (mainly in the stomach) accounts for the bulk of this effect. This "first pass" metabolism (FPM) may be viewed as a barrier which protects against the systemic toxicity of et...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90470-9
更新日期：1985-08-12 00:00:00
abstract：AIMS:We investigated the involvement of the renin angiotensin system (RAS) on the cardiorespiratory control in rats from dams fed with a low-protein diet. MAIN METHODS:Male offspring were obtained from dams fed a normoprotein diet (NP, 17% casein) and low-protein diet (LP, 8% casein) during pregnancy and lactation. Di...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118574
更新日期：2020-12-15 00:00:00
abstract：:Human, dog and rabbit corpus cavernosum type 5 phosphodiesterases (PDE5) were isolated and their characteristics were compared. The three enzymes showed Km values of 0.8, 2.1 and 2.3 uM, respectively. They exhibited similar pH-dependence with optimal pH being 7.5. They required Mg++ for activity and the activity was s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)00989-4
更新日期：2001-03-16 00:00:00
abstract：:Electrically evoked release of dopamine from the caudate nucleus is reduced by the dopamine receptor agonists, apomorphine and bromocriptine, and facilitated by neuroleptic drugs, which act as dopamine autoreceptor antagonists. The potencies of chlorpromazine, fluphenazine, levomepromazine and their hydroxy-metabolite...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90088-9
更新日期：1990-01-01 00:00:00
abstract：:The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.02.023
更新日期：2006-07-10 00:00:00
abstract：AIM:We previously reported the possible involvement of mucosal nitric oxide (NO)-triggered 5-HT release in luminal glutamate sensing by the gastric vagus nerve, and we proposed that the stomach, like the duodenum, could "taste" luminal nutrients. Nitric oxide synthase (NOS) is widely distributed in the gastric mucosa, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.10.009
更新日期：2009-12-16 00:00:00
abstract：AIMS:Despite impressive survival benefits from new agents to treat metastasized prostate cancer (PCa), progressive drug resistance hinders long-term response and restricts the efficacy of subsequent therapy. Due to reported antitumor activity of amygdalin and growing popularity for complementary and alternative medicin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.01.039
更新日期：2016-02-15 00:00:00
abstract：:Regulation of glucose-induced oscillations in intracellular Ca2+ concentration ([Ca2+]i) was investigated by using a novel technique, electroporation from an electrolyte-filled capillary, to deliver energy metabolites to the intracellular compartment of mouse islets. Intracellular application of ATP resulted in a nife...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.05.053
更新日期：2005-10-21 00:00:00
abstract：:Thymol induced superoxide production (O2-) by blood leukocytes was examined in various primates including man. Leukocytes of chimpanzee and hamadryas baboon cells showed only 35% of the maximal O2- production rate obtained in human cells, and those of the Japanese monkey and orang-utan failed to respond. In contrast, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90735-1
更新日期：1987-09-28 00:00:00
abstract：:Metabolic flux through the purine salvage pathway appears to modulate superoxide secretion by elicited macrophages. Exogenous adenosine, the first substrate of this pathway, stimulates superoxide secretion, and Allopurinol, a specific inhibitor of xanthine oxidase, inhibits superoxide secretion. The effects of these a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90811-1
更新日期：1983-03-21 00:00:00
abstract：:Quinolinic acid (QUIN) levels are elevated in patients and animals suffering from chronic infectious diseases. In the present study, male Sprague-Dawley rats were used to test the anti-inflammatory effects of QUIN using the carrageenan (CGN)-induced paw edema assay and the CGN sponge assay. Results of these studies in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00049-1
更新日期：1999-01-01 00:00:00
abstract：AIMS:Perinatal hypoxic-ischemic insult has acute and long term deleterious effects on many organs including heart. Although tumor necrosis factor alpha (TNF-α) has been reported to increase soon after hypoxia, the inhibition of this mediator has not been documented. The aim of this study was to investigate the effects ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.03.036
更新日期：2012-06-06 00:00:00
abstract：:The effects of central administration of two serotonin receptor agonists, 8-OH-DPAT and mCPP, on tuberoinfundibular dopaminergic (TIDA) neuron activity and serum prolactin (PRL) levels in ovariectomized, estrogen-treated rats were determined. 8-OH-DPAT dose-dependently (0.1-10 microgram/rat, icv) stimulated serum PRL ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00077-x
更新日期：1996-01-01 00:00:00
