Abstract:
:In this paper we report on the synthesis of diversified linear polyamine architectures with different chain lengths and compositions and their interaction with phosphate groups of DNA/siRNA. The polyplex formation between model nucleotide (dsDNA) and these linear polyamines has been determined at different nitrogen to phosphorus (N/P) ratios using small-angle neutron scattering (SANS) and atomic force microscopy (AFM) techniques. AFM images showed that while linear poly(ethylene imine) (PEI)/DNA complex results in bigger spherical aggregates, poly(propylene imine)s forms torroid and cigar shaped structures upon complexation with DNA. The poly(butylene imine)s (LPBI)s form compact and soluble DNA complexes with a radii range of R(g) = 15-30 nm. Among the studied linear polyamines, the LPBIs did show the best transfection efficiency.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Fischer W,Brissault B,Prévost S,Kopaczynska M,Andreou I,Janosch A,Gradzielski M,Haag Rdoi
10.1002/mabi.201000082subject
Has Abstractpub_date
2010-09-09 00:00:00pages
1073-83issue
9eissn
1616-5187issn
1616-5195journal_volume
10pub_type
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