Abstract:
:In the last decade, substantial research in the field of post-plasma grafting surface modification has focussed on the introduction of carboxylic acids on surfaces by grafting acrylic acid (AAc). In the present work, we report on an alternative approach for biomaterial surface functionalisation. Thin poly-ε-caprolactone (PCL) films were subjected to a dielectric barrier discharge Ar-plasma followed by the grafting of 2-aminoethyl methacrylate (AEMA) under UV-irradiation. X-ray photoelectron spectroscopy (XPS) confirmed the presence of nitrogen. The ninhydrin assay demonstrated, both quantitatively and qualitatively, the presence of free amines on the surface. Confocal fluorescence microscopy (CFM), atomic force microscopy (AFM) and scanning electron microscopy (SEM) were used to visualise the grafted surfaces, indicating the presence of pAEMA. Static contact angle (SCA) measurements indicated a permanent increase in hydrophilicity. Furthermore, the AEMA grafted surfaces were applied for comparing the physisorption and covalent immobilisation of gelatin. CFM demonstrated that only the covalent immobilisation lead to a complete coverage of the surface. Those gelatin-coated surfaces obtained were further coated using fibronectin. Osteosarcoma cells demonstrated better cell-adhesion and cell-viability on the modified surfaces, compared to the pure PCL films.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Desmet T,Billiet T,Berneel E,Cornelissen R,Schaubroeck D,Schacht E,Dubruel Pdoi
10.1002/mabi.201000147subject
Has Abstractpub_date
2010-12-08 00:00:00pages
1484-94issue
12eissn
1616-5187issn
1616-5195journal_volume
10pub_type
杂志文章abstract::Hydroxypropyl chitosan-graft-carboxymethyl beta-cyclodextrin (HPCH-g-CM beta-CD) was synthesized by grafting CM beta-CD onto HPCH using water soluble 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as the condensing agent. Due to the presence of hydrophobic beta-CD rings onto the HPCH backbone, this polymer can be...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500087
更新日期:2005-10-20 00:00:00
abstract::Multivalent carbohydrate-lectin interactions play a crucial role in bacterial infection. Biomimicry of multivalent glycosystems represents a major strategy in the repression of bacterial growth. In this study, a new kind of glycopeptide (Naphthyl-Phe-Phe-Ser-Tyr, NMY) scaffold with mannose modification is designed and...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900124
更新日期:2019-09-01 00:00:00
abstract::Chitosan is non-toxic, biocompatible, and biodegradable polysaccharide from renewable resources, known to have inherent antibacterial activity, which is mainly due to its polycationic nature. The combining of all assets of chitosan and its derivatives with the unique properties of electrospun nanofibrous materials is ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201300058
更新日期:2013-07-01 00:00:00
abstract::To tailor the erosion rate of polyanhydrides while retaining their surface erosion characteristics, new three-component polyanhydrides of sebacic acid, 1,3-bis(p-carboxyphenoxy)propane and poly(ethylene glycol) were synthesized. The hydrophilicity of the polymer increased and its mechanical strength decreased with inc...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600256
更新日期:2007-05-10 00:00:00
abstract::Nanotechnology is finding ever increasing application in the life science arena where nanoparticles can be used to deliver cargoes in cells. However, a clear understanding of the relationship between the chemical properties of the particle and its uptake efficiency is lacking. Herein, the effects on particle cellular ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200455
更新日期:2013-06-01 00:00:00
abstract::The use of PLA and PLA-g-AA/starch as materials for the controlled release of encapsulated PSB was evaluated. The results showed that the bacterium degraded both the PLA and the PLA-g-AA/starch composite films, resulting in cell release. Severe disruption of the film structure occurred after incubation for 60-90 d. Th...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700181
更新日期:2008-06-11 00:00:00
abstract::Conducting polymers (CPs) is one of intelligent biomaterials with the specific properties of reversible redox states, which have a significant effects on the cell behaviors and nerve tissue regeneration. However, the effects of CPs with different electrical conductivity on the behaviors of nerve cells are rarely repor...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900147
更新日期:2019-10-01 00:00:00
abstract::In this work we report, compare and discuss the results obtained from fully atomistic molecular dynamics simulations of generations 4, 5, and 6 of PAMAM-based dendrimers having NH(3) and triethanolamine as cores, forming complexes with a short interfering RNA (siRNA) at different pH values and at physiological ionic ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100276
更新日期:2012-02-01 00:00:00
abstract::The chain length and end groups of linear PEG grafted on smooth surfaces is known to influence protein adsorption and thrombocyte adhesion. Here, it is explored whether established structure function relationships can be transferred to application relevant, rough surfaces. Functionalization of poly(ether imide) (PEI) ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300309
更新日期:2013-12-01 00:00:00
abstract::A nanoformulation composed of curdlan, a linear polysaccharide of 1,3-β-linked d-glucose units, hydrogen bonded to poly(γ -glutamic acid) (PGA), was developed to stimulate macrophage. Curdlan/PGA nanoparticles (C-NP) are formulated by physically blending curdlan (0.2 mg mL-1 in 0.4 m NaOH) with PGA (0.8 mg mL-1 ). For...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000358
更新日期:2021-01-01 00:00:00
abstract::This paper presents the analysis of the volume phase transition of ionic thermo-sensitive hydrogels to thermal stimulus through mathematical modelling. The model is termed the multi-effect-coupling thermal-stimulus (MECtherm) model and it considers the effects of multi-phases and multi-physics. Its application to stea...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500082
更新日期:2005-09-16 00:00:00
abstract::The supramolecular self-assembly of macromonomers may serve as a first step to prepare well-defined, highly functionalized, hierarchically structured, conjugated polymers. Functional diacetylene macromonomers equipped with an oligopeptide segment designed to promote self-assembly into parallel beta-sheet type structur...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600226
更新日期:2007-02-12 00:00:00
abstract::Novel molecular ecological techniques were used to study changes in microbial community structure and population during degradation of polylactide (PLA)/organically modified layered silicates (OMLS) nanocomposites. Cloned gene sequences belonging to members of the phyla Actinobacteria and Ascomycota comprized the most...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800276
更新日期:2009-07-07 00:00:00
abstract::This study presents the reactive self-assembly of isocyanate functional and amphiphilic six-arm, star-shaped polyether prepolymers in water into nanogels. Intrinsic molecular amphiphilicity, mainly driven by the isophorone moiety at the distal endings of the star-shaped molecules, allows for the preparation of spheric...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800094
更新日期:2018-10-01 00:00:00
abstract::The immobilization of enzymes into polymer hydrogels is a versatile approach to improve their stability and utility in biotechnological and biomedical applications. However, these systems typically show limited enzyme activity, due to unfavorable pore dimensions and low enzyme accessibility. Here, 3D jet writing of wa...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000154
更新日期:2020-09-01 00:00:00
abstract::Implant failure is one of the major concerns in the biomaterials field. Several factors have been related to the fail but in general these biomaterials do not exhibit comparable physical, chemical or biological properties to natural tissues and ultimately, these devices can lead to chronic inflammation and foreign-bod...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500010
更新日期:2005-08-12 00:00:00
abstract::The synthesis of a new glycomonomer based on mannose, prepared via CuAAC, is reported. The resulting 1,2,3-triazole linkage between mannose and the polymer backbone ensures the formation of highly stable glycopolymers, which will not undergo hydrolysis. The monomer 2'-(4-vinyl-[1,2,3]-triazol-1-yl)ethyl-O-alpha-D-mann...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900199
更新日期:2010-02-11 00:00:00
abstract::Chronic non-healing wounds show delayed and incomplete healing processes and in turn expose patients to a high risk of infection. Treatment currently focuses on dressings that prevent microbial infiltration and keep a balanced moisture and gas exchange environment. Antibacterial delivery from dressings has existed for...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201300561
更新日期:2014-06-01 00:00:00
abstract::The antiviral effects of the cationic acrylate polymer E100 on a panel of lipid-enveloped viruses and the interactions involved are studied. The treatment of several common viruses with E100 induced a dose-dependent inhibition of the infectivity of viruses below the detection limit of the assays employed. Similarly, t...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700102
更新日期:2007-09-11 00:00:00
abstract::Manipulated roughness has been etched on nanofibers to modulate adsorption of serum proteins for serum-free cells cultivation. Mixtures of Polycaprolactone and Pluronic with various blending ratios are electrospun to nanofibers and Pluronic is subsequently leached out by methanol. Electron microscopy reveals that surf...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700057
更新日期:2017-08-01 00:00:00
abstract::Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200471
更新日期:2013-05-01 00:00:00
abstract::A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported. For reinforcing ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700176
更新日期:2017-11-01 00:00:00
abstract::In-depth understanding of the biophysicochemical interactions at the nano-bio interface is important for basic cell biology and applications in nanomedicine and nanobiosensors. Here, the extracellular surface potential and topography changes of live cell membranes interacting with polymeric nanomaterials using a scann...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800271
更新日期:2019-02-01 00:00:00
abstract::Two different composite scaffolds, solid-freeform-fabricated PCL/β-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular ac...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200393
更新日期:2013-05-01 00:00:00
abstract::A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins w...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100351
更新日期:2012-07-01 00:00:00
abstract::A series of enzymatically recyclable poly(ester-urethane)s consisting of a biodegradable diurethane moiety as a hard segment and an ester moiety as an enzymatically cleavable linkage was chemo-enzymatically prepared by two routes. The poly(ester-urethane) was prepared by a) the ring-opening polymerization of a cyclic ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400176
更新日期:2005-04-19 00:00:00
abstract::Multicellular tumor spheroid (MCTS) mimics microenvironment for tumor formation and provides predictive insight for in vivo tests. The hanging drop (HD) method of spheroid generation is cost effective, but it is limited by a long time duration for spheroid development and a low rate of formation of larger spheroids. T...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000180
更新日期:2020-08-01 00:00:00
abstract::Biodegradable hydrogels were synthesized by the click reaction of 4-arm azido-terminated PEG differing in molecular weight (2,100 and 8,800) and two alkyne-terminated peptides: [alkyne]-GFLGK-[alkyne] and ([alkyne]-GFLG)(2)K. The physical properties of in situ formed hydrogels were examined. The hydrogels were highly ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900295
更新日期:2010-04-08 00:00:00
abstract::In this study we formed and characterized dynamic hydrogel microspheres in which a protein conformational change was used to control microsphere volume changes and the release of an encapsulated drug. In particular, a specific biochemical ligand, trifluoperazine, induced calmodulin's nanometer scale conformation chang...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900382
更新日期:2010-06-11 00:00:00
abstract::"Three-layered micelles" (3LM) composed of two triblock copolymers, poly(L-lactide)-b-polyethyleneimine-b-poly(L-lactide) (PLLA-PEI-PLLA) and poly(L-lactide)-b-poly(ethylene glycol)-b-poly(L-lactide) (PLLA-PEG-PLLA) are designed to combine electrostatic interaction and solvent-induced condensation of DNA. The low mole...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201400488
更新日期:2015-05-01 00:00:00