Autoinducer Sensing Microarrays by Reporter Bacteria Encapsulated in Hybrid Supramolecular-Polysaccharide Hydrogels.

Abstract:

:A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported. For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking. The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating. Templating affords reporter bacteria-loaded hydrogels with controllable shape and size. Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression. This approach can serve as a potentially generally applicable strategy to sensitively detect bacteria via their secreted autoinducers.

journal_name

Macromol Biosci

authors

Li P,Dou X,Müller M,Feng C,Chang MW,Frettlöh M,Schönherr H

doi

10.1002/mabi.201700176

subject

Has Abstract

pub_date

2017-11-01 00:00:00

issue

11

eissn

1616-5187

issn

1616-5195

journal_volume

17

pub_type

杂志文章
  • Strong fibers and films of microbial polyesters.

    abstract::Poly[(R)-3-hydroxybutyrate] (P(3HB)) and its copolymers are accumulated by a wide variety of microorganisms as intracellular carbon and energy material, and are extensively studied as biodegradable and biocompatible thermoplastics. However, these microbial polyesters have not been recognized as practical because of th...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章,评审

    doi:10.1002/mabi.200500066

    authors: Iwata T

    更新日期:2005-08-12 00:00:00

  • Nanofiber formation of hydroxylpropylcellulose (HPC).

    abstract::The aggregative behaviors of hydroxypropylcellulose (HPC) molecules in aqueous solution and on substrates have been observed by employing laser light scattering (LLS) and, after deposition on a mica surface, atomic force microscopy (AFM). LLS studies showed that the HPC molecules formed large aggregates through self-a...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200600067

    authors: Yan L,Lin W,Bangal PR

    更新日期:2006-07-14 00:00:00

  • A Novel Small-Caliber Bacterial Cellulose Vascular Prosthesis: Production, Characterization, and Preliminary In Vivo Testing.

    abstract::Vascular grafts are used to bypass damaged or diseased blood vessels. Bacterial cellulose (BC) has been studied for use as an off-the-shelf graft. Herein, we present a novel, cost-effective, method for the production of small caliber BC grafts with minimal processing or requirements. The morphology of the graft wall p...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201500251

    authors: Leitão AF,Faria MA,Faustino AM,Moreira R,Mela P,Loureiro L,Silva I,Gama M

    更新日期:2016-01-01 00:00:00

  • CHO cells adhering to nitrogen-rich plasma-polymerised ethylene exhibit high production of a specific recombinant protein.

    abstract::In many industrial applications, inadequate cell attachment can be a limitation, especially when serum-free media are used. Nitrogen-rich plasma-polymerised ethylene (PPE:N) exhibits high concentrations of polar groups that can help to promote the attachment of weakly adherent cell types. Tissue plasminogen activator-...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200900079

    authors: Gigout A,Levasseur S,Girard-Lauriault PL,Buschmann MD,Wertheimer MR,Jolicoeur M

    更新日期:2009-10-08 00:00:00

  • Amylose's recognition of chirality in polylactides on formation of inclusion complexes in vine-twining polymerization.

    abstract::Amylose selectively includes poly(L-lactide) (PLLA) among the poly(lactide)s (PLAs) to produce an inclusion complex when the phosphorylase-catalyzed polymerization of α-D-glucose 1-phosphate is performed in the presence of PLLA, poly(D-lactide) (PDLA), or poly(DL-lactide) (PDLLA) (vine-twining polymerization). This re...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100133

    authors: Kaneko Y,Ueno K,Yui T,Nakahara K,Kadokawa J

    更新日期:2011-10-10 00:00:00

  • Characterization of Hydrogels Made of a Novel Spider Silk Protein eMaSp1s and Evaluation for 3D Printing.

    abstract::Recombinantly produced spider silk proteins have high potential for bioengineering and various biomedical applications because of their biocompatibility, biodegradability, and low immunogenicity. Here, the recently described small spider silk protein eMaSp1s is assembled into hydrogels, which can be 3D printed into sc...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201700141

    authors: Thamm C,DeSimone E,Scheibel T

    更新日期:2017-11-01 00:00:00

  • Development of mucoadhesive drug delivery system using phenylboronic acid functionalized poly(D,L-lactide)-b-dextran nanoparticles.

    abstract::Mucoadhesive NP drug carriers have attracted substantial interest as a potential treatment for anterior eye diseases. NPs composed of PLA-Dex surface functionalized with a mucoadhesive ligand, PBA, were developed as drug carriers with particle sizes ranging from 25 to 28 nm. Using CycA as a model drug, we showed that ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200216

    authors: Liu S,Jones L,Gu FX

    更新日期:2012-12-01 00:00:00

  • Fluoropolymer based on a polyaspartamide containing 1,2,4-oxadiazole units: a potential artificial oxygen (O2) carrier.

    abstract::In this preliminary work we have prepared a fluorinated polymer capable of solubilizing an appreciable amount of O(2) and, at the same time, maintaining a higher water solubility than perfluoroalkanes investigated as injectable O(2) carriers. In particular, we describe the synthesis and characterization of a new macro...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200600266

    authors: Mandracchia D,Piccionello AP,Pitarresi G,Pace A,Buscemi S,Giammona G

    更新日期:2007-06-07 00:00:00

  • Stereoblock poly(lactic acid): synthesis via solid-state polycondensation of a stereocomplexed mixture of poly(L-lactic acid) and poly(D-lactic acid).

    abstract::Stereoblock poly(lactic acid) consisting of D- and L-lactate stereosequences can be successfully synthesized by solid-state polycondensation of a 1:1 mixture of poly(L-lactic acid) and poly(D-lactic acid). In the first step, melt-polycondensation of L- and D-lactic acids is conducted to synthesize poly(L-lactic acid) ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200400121

    authors: Fukushima K,Furuhashi Y,Sogo K,Miura S,Kimura Y

    更新日期:2005-01-14 00:00:00

  • Oxidative Epigallocatechin Gallate Coating on Polymeric Substrates for Bone Tissue Regeneration.

    abstract::Plant derived flavonoids have not been well explored in tissue engineering applications due to difficulties in efficient formulations with biomaterials for controlled presentation. Here, the authors report that surface coating of epigallocatechin gallate (EGCG) on polymeric substrates including poly (L-lactic acid) (P...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201800392

    authors: Madhurakkat Perikamana SK,Lee SM,Lee J,Ahmad T,Lee MS,Yang HS,Shin H

    更新日期:2019-04-01 00:00:00

  • Reactive Self-Assembly and Specific Cellular Delivery of NCO-sP(EO-stat-PO)-Derived Nanogels.

    abstract::This study presents the reactive self-assembly of isocyanate functional and amphiphilic six-arm, star-shaped polyether prepolymers in water into nanogels. Intrinsic molecular amphiphilicity, mainly driven by the isophorone moiety at the distal endings of the star-shaped molecules, allows for the preparation of spheric...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201800094

    authors: Hildebrandt H,Paloheimo O,Mäntylä E,Willman S,Hakanen S,Albrecht K,Groll J,Möller M,Vihinen-Ranta M

    更新日期:2018-10-01 00:00:00

  • Isolation and structure determination of complexed poly(3-hydroxyalkanoate) from beet (Beta vulgaris L.).

    abstract::Complexed poly(3-hydroxyalkanoate)s (cPHAs), one of two types of natural PHAs, occur in both prokaryotes and eukaryotes as a complex with biomacromolecules and could be involved in various physiological functions. In this study, a cPHA-component derived from a complex with calcium polyphosphate was isolated from sugar...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200500111

    authors: Suzuki Y,Esumi Y,Koshino H,Doi Y

    更新日期:2005-09-16 00:00:00

  • Nonionic Dendritic and Carbohydrate Based Amphiphiles: Self-Assembly and Transport Behavior.

    abstract::Herein, a new series of non-ionic dendritic and carbohydrate based amphiphiles is synthesized employing biocompatible starting materials and studied for supramolecular aggregate formation in aqueous solution. The dendritic amphiphiles 12 and 13 possessing poly(glycerol) [G2.0] as hydrophilic unit and C-10 and C-18 hyd...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201800019

    authors: Prasad S,Achazi K,Schade B,Haag R,Sharma SK

    更新日期:2018-07-01 00:00:00

  • Cell-Laden Hydrogels for Multikingdom 3D Printing.

    abstract::Living materials are created through the embedding of live, whole cells into a matrix that can house and sustain the viability of the encapsulated cells. Through the immobilization of these cells, their bioactivity can be harnessed for applications such as bioreactors for the production of high-value chemicals. While ...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000121

    authors: Johnston TG,Fillman JP,Priks H,Butelmann T,Tamm T,Kumar R,Lahtvee PJ,Nelson A

    更新日期:2020-08-01 00:00:00

  • Non-adhesive behavior of new nanostructured PNIPAM surfaces towards specific neurodegenerative proteins: application to storage and titration of Tau proteins.

    abstract::New nonfouling tubes are developed and their influence on the adhesion of neuroproteins is studied. The biomarkers are considered as single components (recombinant prion and Tau proteins) or in a solution of native and pathological forms. The samples are stored for 24 h at 4 °C in virgin and treated tubes layered with...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200116

    authors: Vrlinic T,Debarnot D,Legeay G,Coudreuse A,El Moualij B,Zorzi W,Perret-Liaudet A,Quadrio I,Mozetic M,Poncin-Epaillard F

    更新日期:2012-10-01 00:00:00

  • Evaluation of Surface Microtopography Engineered by Direct Laser Interference for Bacterial Anti-Biofouling.

    abstract::Modification of the biomaterial surface topography is a promising strategy to prevent bacterial adhesion and biofilm formation. In this study, we use direct laser interference patterning (DLIP) to modify polystyrene surface topography at sub-micrometer scale. The results revealed that three-dimensional micrometer stru...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201500107

    authors: Valle J,Burgui S,Langheinrich D,Gil C,Solano C,Toledo-Arana A,Helbig R,Lasagni A,Lasa I

    更新日期:2015-08-01 00:00:00

  • Development and Characterization of Ultrasound Activated Lipopolyplexes for Enhanced Transfection by Low Frequency Ultrasound in In Vitro Tumor Model.

    abstract::This work focuses on the development of ultrasound contrast vesicles for ultrasound-mediated enhanced transfection of nucleic acids in the cancer cells and projects its application as a tool for diagnostic imaging. The ultrasound contrast vesicles are stable, anionic, nanoscaled vesicles with ultrasound contrast equiv...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000173

    authors: Shah H,Tariq I,Engelhardt K,Bakowsky U,Pinnapireddy SR

    更新日期:2020-12-01 00:00:00

  • Vitamin Bc -Bearing Hydrophilic Photosensitizer Conjugate for Photodynamic Cancer Theranostics.

    abstract::The accurate diagnosis and proper therapy for cancer are essential to improve the success rate of cancer treatment. Here, we demonstrated that the vitamin Bc -bearing hydrophilic photosensitizer conjugate folic acid-polyethylene glycol-pheophorbideA (FA-PEG-PheoA) has been synthesized for the intracellular diagnosis a...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201500060

    authors: Kim J,Kim KS,Park SJ,Na K

    更新日期:2015-08-01 00:00:00

  • Poly(anhydride-ester) and poly(N-vinyl-2-pyrrolidone) blends: salicylic acid-releasing blends with hydrogel-like properties that reduce inflammation.

    abstract::Polymers such as poly(N-vinyl-2-pyrrolidone) (PVP) have been used to prepare hydrogels for wound dressing applications but are not inherently bioactive. For enhanced healing, PVP was blended with salicylic acid-based poly(anhydride-esters) (SAPAE) and shown to exhibit hydrogel properties upon swelling. In vitro releas...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201400238

    authors: Ouimet MA,Fogaça R,Snyder SS,Sathaye S,Catalani LH,Pochan DJ,Uhrich KE

    更新日期:2015-03-01 00:00:00

  • Crosslinking of trimethylene carbonate and D, L-lactide (co-) polymers by gamma irradiation in the presence of pentaerythritol triacrylate.

    abstract::High-molecular-weight (co)polymers of trimethylene carbonate and D,L-lactide are efficiently crosslinked using PETA during gamma irradiation. Form-stable networks with gel contents of 86 ± 5 to 96 ± 1 are obtained from non-crystalline (co)polymers. Glass transition temperatures and elastic moduli of the networks can b...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100031

    authors: Bat E,van Kooten TG,Feijen J,Grijpma DW

    更新日期:2011-07-07 00:00:00

  • Synthesis and Validation of Functional Nanogels as pH-Sensors in the Hair Follicle.

    abstract::In the present study, a pH responsive dendritic polyglycerol nanogel (dPG-NG) is developed to measure the pH values inside the hair follicle (HF) using an ex vivo porcine ear model. The macromolecular precursors are labeled with a pH sensitive indodicarbocyanine dye (pH-IDCC) and a control dye (indocarbocyanine dye: I...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201600505

    authors: Dimde M,Sahle FF,Wycisk V,Steinhilber D,Camacho LC,Licha K,Lademann J,Haag R

    更新日期:2017-10-01 00:00:00

  • Non-woven fibrous materials with antibacterial properties prepared by tailored attachment of quaternized chitosan to electrospun mats from maleic anhydride copolymer.

    abstract::In order to impart antibacterial properties to microfibrous electrospun materials from styrene/maleic anhydride copolymers, quaternized chitosan derivatives (QCh) containing alkyl substituents of different chain lengths are covalently attached to the mats. A complete inhibition of the growth of bacteria, S. aureus (Gr...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201100178

    authors: Ignatova M,Petkova Z,Manolova N,Markova N,Rashkov I

    更新日期:2012-01-01 00:00:00

  • Mastoid obliteration using three-dimensional composite scaffolds consisting of polycaprolactone/β-tricalcium phosphate/collagen nanofibers: an in vitro and in vivo study.

    abstract::Two different composite scaffolds, solid-freeform-fabricated PCL/β-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular ac...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200393

    authors: Jang CH,Cho YB,Yeo MG,Kim GH

    更新日期:2013-05-01 00:00:00

  • Poly(N-methylvinylamine)-Based Copolymers for Improved Gene Transfection.

    abstract::Poly(N-methylvinylamines) with secondary amines can form complexes with plasmid DNA (pDNA) and provide transfection efficiency in HeLa cells in the same order as linear polyethyleneimine but with higher cell viability. Chemical modifications of poly(N-methylvinylamine) backbones are performed to further improve transf...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201700353

    authors: Dréan M,Debuigne A,Jérôme C,Goncalves C,Midoux P,Rieger J,Guégan P

    更新日期:2018-04-01 00:00:00

  • Formation of wheat-protein-based biomaterials through polymer grafting and crosslinking reactions to introduce new functional properties.

    abstract::An insoluble wheat proteins-based polymer networks with new functional groups and positive charges were prepared through grafting-coupling reactions. The networks were also flexible with a lower glass transition temperature since the existence of the grafted mobile segments. The multi-component/multi-phase systems bec...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200800156

    authors: Kurniawan L,Qiao GG,Zhang X

    更新日期:2009-01-09 00:00:00

  • Improved anti-inflammatory and pharmacokinetic properties for superoxide dismutase by chemical glycosidation with carboxymethylchitin.

    abstract::O-carboxymethylchitin (molecular weight = 1.07 x 10(5), degree of carboxymethylation = 80%, degree of N-acetylation = 91%) was chemically attached to superoxide dismutase by the formation of amide linkages through a carbodiimide catalyzed reaction. The glycosidated enzyme contained about 1.8 mole of polysaccharide per...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200400114

    authors: Valdivia A,Perez Y,Dominguez A,Caballero J,Gomez L,Schacht EH,Villalonga R

    更新日期:2005-02-23 00:00:00

  • Quantifying Thermoswitchable Carbohydrate-Mediated Interactions via Soft Colloidal Probe Adhesion Studies.

    abstract::Thermosensitive polymers enable externally controllable biomolecular interactions but hysteresis effects hamper the reversibility and repeated use of these materials. To quantify the temperature-dependent interactions and hysteresis effects, an optical adhesion assay based on poly(ethylene glycol) microgels (soft coll...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.202000186

    authors: Strzelczyk AK,Paul TJ,Schmidt S

    更新日期:2020-10-01 00:00:00

  • Thermosensitive macroporous cryogels functionalized with bioactive chitosan/bemiparin nanoparticles.

    abstract::Thermosensitive macroporous scaffolds of poly(N-isopropylacrylamide) (polyNIPA) loaded with chitosan/bemiparin nanoparticles are prepared by the free radical polymerization in cryogenic conditions. Chitosan/bemiparin nanoparticles of 102 ± 6.5 nm diameter are prepared by complex coacervation and loaded into polyNIPA c...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201300184

    authors: Peniche H,Reyes-Ortega F,Aguilar MR,Rodríguez G,Abradelo C,García-Fernández L,Peniche C,San Román J

    更新日期:2013-11-01 00:00:00

  • Functionalized polystyrene nanoparticles trigger human dendritic cell maturation resulting in enhanced CD4+ T cell activation.

    abstract::Nanoparticles (NP) represent a promising tool for biomedical applications. Here, sulfonate- and phosphonate-functionalized polystyrene NP are analyzed for their interaction with human monocyte-derived dendritic cells (DC). Immature dendritic cells (iDC) display a higher time- and dose-dependent uptake of functionalize...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.201200223

    authors: Frick SU,Bacher N,Baier G,Mailänder V,Landfester K,Steinbrink K

    更新日期:2012-12-01 00:00:00

  • Response of human corneal fibroblasts on silk film surface patterns.

    abstract::Transparent, biodegradable, mechanically robust, and surface-patterned silk films were evaluated for the effect of surface morphology on human corneal fibroblast (hCF) cell proliferation, orientation, and ECM deposition and alignment. A series of dimensionally different surface groove patterns were prepared from optic...

    journal_title:Macromolecular bioscience

    pub_type: 杂志文章

    doi:10.1002/mabi.200900452

    authors: Gil ES,Park SH,Marchant J,Omenetto F,Kaplan DL

    更新日期:2010-06-11 00:00:00