Abstract:
:Poly(N-methylvinylamines) with secondary amines can form complexes with plasmid DNA (pDNA) and provide transfection efficiency in HeLa cells in the same order as linear polyethyleneimine but with higher cell viability. Chemical modifications of poly(N-methylvinylamine) backbones are performed to further improve transfection efficiency while maintaining low degree of cytotoxicity. In a first type of polymer, primary amino groups are incorporated via a copolymerization strategy. In a second one, primary amino and imidazole groups are incorporated also via a copolymerization strategy. In a third one, secondary amino groups are substituted with methylguanidine functions through a postpolymerization reaction. Thus, novel polymers of various molecular masses are synthesized, characterized, and their interaction with pDNA studied. Then, their transfection efficiency and cytotoxicity are tested in HeLa cells. Two polymethylvinylamine-based copolymers, one containing 20% of imidazole moieties and another one composed of 12% of guanidinyl units allow remarkable transfection efficiency of HeLa, pulmonary (16HBE), skeletal muscle (C2C12), and dendritic (DC2.4) cells. Overall, this work thus identifies new promising DNA carriers and chemical modifications that improve the transfection efficiency while maintaining low degree of cytotoxicity.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Dréan M,Debuigne A,Jérôme C,Goncalves C,Midoux P,Rieger J,Guégan Pdoi
10.1002/mabi.201700353subject
Has Abstractpub_date
2018-04-01 00:00:00pages
e1700353issue
4eissn
1616-5187issn
1616-5195journal_volume
18pub_type
杂志文章abstract::Injectable hydrogels are extensively used in drug delivery and tissue engineering to administer drugs, genes, growth factors and live cells. We report a method to produce tough, in-situ thermogelling, non-toxic, injectable hydrogels made of chitosan and hyaluronic acid co-crosslinked with β-glycerophophate and genipin...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201400406
更新日期:2015-04-01 00:00:00
abstract::Bioinks play a key role in determining the capability of the biofabricatoin processes and the resolution of the printed constructs. Excellent biocompatibility, tunable physical properties, and ease of chemical or biological modifications of gelatin methacryloyl (GelMA) have made it an attractive choice as bioinks for ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000317
更新日期:2021-01-01 00:00:00
abstract::Basic fibroblast growth factor (bFGF) is an important protein for wound healing and angiogenesis in tissue engineering, but the lack of a viable delivery system hampers its clinical application. This study aims to maintain the long-term controlled release of bFGF by utilizing a collagen/heparin bi-affinity multilayer ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800086
更新日期:2018-11-01 00:00:00
abstract::BSA adsorption onto negatively and positively charged polystyrene nanoparticles was investigated. The nanoparticles were characterized in terms of particle size, zeta potential, surface group density, and morphology. The adsorption behavior of BSA on the particle surface, as a function of pH and overall charge of the ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000395
更新日期:2011-05-12 00:00:00
abstract::Emphasizing the role of hydrogel stiffness and cellular differentiation, this study develops collagen and elastin-like polypeptide (ELP)-based bone regenerative hydrogels loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) and doxycycline with mechanical properties suitable for osteogenesis. The drug-...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900142
更新日期:2019-09-01 00:00:00
abstract::Although recently a growing number of reports demonstrate that topography or geometry of the substrate also plays an important role in the fate of the stem cells, most of these studies are usually completed by a few distinct patterns such as simple lines, posts, etc. As a result, there is a lack of quantitative analys...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000477
更新日期:2011-07-07 00:00:00
abstract::The design of a novel polymer-modified overlayer composed of PPAPE and GPMS on a silicon wafer for immobilization of DNA molecules is described. After hydroxylation of Si(100) surfaces, GPMS molecules were self-assembled onto these surfaces. PPAPE molecules were then covalently attached to the epoxy-terminated surface...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800226
更新日期:2009-05-13 00:00:00
abstract::The present study describes the synthesis of novel polypeptides containing O-phospho-L-threonine [Thr(PO(3)H(2))] and L-aspartic acid. Random copolypeptides copoly[Thr(PO(3)H(2))(X)Asp(Y)] (X:Y = 25:75, 50:50, 75:25), were conventionally prepared by copolymerization of Thr(PO(3)Ph(2)) N-carboxyanhydride (NCA) and Asp(...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500211
更新日期:2006-03-14 00:00:00
abstract::A new anhydroribotrisaccharide monomer, A2B3LR (1), was synthesized and ROP was carried out to elucidate the polymerizability and to obtain oligosaccharide-branched polysaccharides with defined structures. The new trisaccharide monomer was found to be polymerized readily with BF(3) . OEt(2) as a catalyst at -40 degree...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800316
更新日期:2009-07-07 00:00:00
abstract::Surface structural characterisation of three different starch-based blends (with poly[ethylene-co-(vinyl alcohol)], cellulose acetate and polycaprolactone) was carried out. The results show that there is a difference between the bulk and the surface composition of all studied blends. Two different hypotheses were inve...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700076
更新日期:2008-02-11 00:00:00
abstract::Vascular grafts are used to bypass damaged or diseased blood vessels. Bacterial cellulose (BC) has been studied for use as an off-the-shelf graft. Herein, we present a novel, cost-effective, method for the production of small caliber BC grafts with minimal processing or requirements. The morphology of the graft wall p...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500251
更新日期:2016-01-01 00:00:00
abstract::The use of glycopolymer-functionalized resins (Resin-Glc), as a solid support, in column mode for bacterial/protein capture and quantification is explored. The Resin-Glc is synthesized from commercially available chloromethylated polystyrene resin and glycopolymer, and is characterized by fourier transform infrared sp...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000342
更新日期:2020-12-18 00:00:00
abstract::Extraction processes of natural fibers can be performed by different procedures that include mechanical, chemical and biological methods. Each method presents different advantages or drawbacks according to the amount of fiber produced or the quality and properties of fiber bundles obtained. In this study, biological n...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400041
更新日期:2004-10-20 00:00:00
abstract::When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue" that binds strongly and q...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200092
更新日期:2012-08-01 00:00:00
abstract::A poly(o-methoxyaniline) (POMA)/DNA [weight fraction of DNA (W(DNA)) = 0.45] hybrid was prepared by mixing their solutions in sterilized double distilled water. The solution turned green upon aging for a longer time, and the doping of POMA by DNA was complete after about 15 d of aging. The doping was confirmed from th...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400185
更新日期:2005-05-23 00:00:00
abstract::Living materials are created through the embedding of live, whole cells into a matrix that can house and sustain the viability of the encapsulated cells. Through the immobilization of these cells, their bioactivity can be harnessed for applications such as bioreactors for the production of high-value chemicals. While ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000121
更新日期:2020-08-01 00:00:00
abstract::The effects of RGD peptide conjugation to alginate hydrogel on the adipogenic differentiation of ASCs was investigated. After 3 d of culture, RGD-modified alginate hydrogels significantly stimulated FAK and integrin α1 gene expressions and vinculin expression in ASCs. In addition, RGD-modified alginate hydrogels signi...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000479
更新日期:2011-05-12 00:00:00
abstract::Manipulated roughness has been etched on nanofibers to modulate adsorption of serum proteins for serum-free cells cultivation. Mixtures of Polycaprolactone and Pluronic with various blending ratios are electrospun to nanofibers and Pluronic is subsequently leached out by methanol. Electron microscopy reveals that surf...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700057
更新日期:2017-08-01 00:00:00
abstract::The synthesis of a zwitterionic polymer, poly-sulfobetaine (poly-SPB), which shows remarkable efficiency in the suppression of insulin aggregation is described. Hydrophobic modification of the polymer results in almost complete inhibition at very low polymer concentrations. Further studies reveal that these polymers f...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800016
更新日期:2018-06-01 00:00:00
abstract::The supramolecular self-assembly of macromonomers may serve as a first step to prepare well-defined, highly functionalized, hierarchically structured, conjugated polymers. Functional diacetylene macromonomers equipped with an oligopeptide segment designed to promote self-assembly into parallel beta-sheet type structur...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600226
更新日期:2007-02-12 00:00:00
abstract::Metastasis is a pathogenic spread of cancer cells from the primary site to surrounding tissues and distant organs, making it one of the primary challenges for effective cancer treatment and the major cause of cancer mortality. Heparin-based biomaterials exhibit significant inhibition of cancer cell metastasis. In this...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800409
更新日期:2019-05-01 00:00:00
abstract::Given the major structural role phosphodiesters play in the organism it is surprising they have not been more widely adopted as a building block in sophisticated biomimetic hydrogels and other biomaterials. The potential benefits are substantial: phosphoester-based materials show excellent compatibility with blood, ce...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900090
更新日期:2019-07-01 00:00:00
abstract::Mucoadhesive NP drug carriers have attracted substantial interest as a potential treatment for anterior eye diseases. NPs composed of PLA-Dex surface functionalized with a mucoadhesive ligand, PBA, were developed as drug carriers with particle sizes ranging from 25 to 28 nm. Using CycA as a model drug, we showed that ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200216
更新日期:2012-12-01 00:00:00
abstract::Current methods of tissue fixation rely on mechanical-related technologies developed from the clothing and carpentry industries. Herein, a novel bioadhesive method that allows tuneable adhesion and is also applicable to biodegradable polyester substrates is described. Diazirine is the key functional group that allows ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300380
更新日期:2014-04-01 00:00:00
abstract::Local, micromechanical environment is known to influence cellular function in heterogeneous hydrogels, and knowledge gained in micromechanics will facilitate the improved design of biomaterials for tissue regeneration. In this study, a system comprising microstructured resilin-like polypeptide (RLP)-poly(ethylene glyc...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900360
更新日期:2020-05-01 00:00:00
abstract::The biorecognition-based control of attachment/detachment of MCF-7 cancer cells from polymer-coated surfaces is demonstrated. A glass surface is coated with a thermoresponsive statistical copolymer of poly(N-isopropylacrylamide-co-acrylamide) [p(NIPAm-co-Am)], which is end-capped with the Gly-Arg-Gly-Asp-Ser (GRGDS) p...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000277
更新日期:2020-11-04 00:00:00
abstract::Stereolithography-assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω-methacrylate poly(d,l-lactide-block-ethylene glycol-block- d,l-lactide) (MA-PDLLA-PEG-PDLLA-MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800403
更新日期:2019-04-01 00:00:00
abstract::Polymeric nanostructures obtained through self-assembly of reduction-sensitive amphiphilic triblock copolymers were investigated as potential drug delivery systems. The characteristic feature of these polymers is their cleavable disulfide bond in the center of the hydrophobic block. Therefore, the triblock copolymers ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201400400
更新日期:2015-04-01 00:00:00
abstract::The present review is aimed at elucidating relatively new aspects of mucoadhesion/mucus interaction and related phenomena that emerged from a Mucoadhesion workshop held in Munster on 2-3 September 2015 as a satellite event of the ICCC 13th-EUCHIS 12th. After a brief outline of the new issues, the focus is on mucus des...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201600534
更新日期:2017-08-01 00:00:00
abstract::Open-pore biodegradable foams with controlled porous architectures were prepared by combining gas foaming and microparticulate templating. Microparticulate composites of poly(epsilon-caprolactone) (PCL) and micrometric sodium chloride particles (NaCl), in concentrations ranging from 70/30 to 20/80 wt.-% of PCL/NaCl we...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700278
更新日期:2008-07-07 00:00:00