Abstract:
:BSA adsorption onto negatively and positively charged polystyrene nanoparticles was investigated. The nanoparticles were characterized in terms of particle size, zeta potential, surface group density, and morphology. The adsorption behavior of BSA on the particle surface, as a function of pH and overall charge of the particle, was studied using ITC. Different thermodynamic data such as enthalpy changes upon binding and stoichiometry of the systems were determined and discussed. The degree of surface coverage with BSA was calculated using the thermodynamic data. The cellular uptake of particles before and after BSA adsorption was studied using HeLa cells in the presence and absence of supplemented FCS in the cell culture medium.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Baier G,Costa C,Zeller A,Baumann D,Sayer C,Araujo PH,Mailänder V,Musyanovych A,Landfester Kdoi
10.1002/mabi.201000395subject
Has Abstractpub_date
2011-05-12 00:00:00pages
628-38issue
5eissn
1616-5187issn
1616-5195journal_volume
11pub_type
杂志文章abstract::Highly crystalline amylose-polytetrahydrofuran (PTHF) complexes can be obtained by employing organic solvents as washing agents after complex formation. The X-ray diffraction (XRD) of the washed complexes appear sharp at 12.9°-13.2° and 19.6°-20.1°, clear signs of the presence of V6I -amylose. Other diffraction peaks ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300174
更新日期:2014-01-01 00:00:00
abstract::Three different microbial wild-type strains are compared with respect to their potential as industrial scale polyhydroxyalkanoate (PHA) producers from the feed stock whey lactose. The halophilic archaeon Haloferax mediterranei as well as two eubacterial strains (Pseudomonas hydrogenovora and Hydrogenophaga pseudoflava...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600211
更新日期:2007-02-12 00:00:00
abstract::The properties of poly(D,L-lactide)-block-poly(2-hydroxyethyl acrylate) (PLA-b-PHEA) block copolymers by means of in vitro / in vivo (rat) degradation are investigated and compared to those of PLA homopolymer. Over 12 weeks, we observe mass loss and molecular weight decrease. In vitro and in vivo findings are very sim...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100067
更新日期:2011-09-09 00:00:00
abstract::In this paper we report on the synthesis of diversified linear polyamine architectures with different chain lengths and compositions and their interaction with phosphate groups of DNA/siRNA. The polyplex formation between model nucleotide (dsDNA) and these linear polyamines has been determined at different nitrogen to...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000082
更新日期:2010-09-09 00:00:00
abstract::The naturally occurring edible biopolymer poly(gamma-glutamic acid) (gamma-PGA) is shown to be an efficient chelating agent of vanadium(IV). The structure of poly(gamma-glutamic acid)oxovanadium(IV) (VO-gamma-PGA) complex in solution has been analyzed by electron spin resonance and UV-visible absorption spectra. The e...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700121
更新日期:2008-02-11 00:00:00
abstract::Mucins are high molecular-weight glycoproteins having oligosaccharides attached to serine or threonine residues of the mucin core protein backbone by O-glycosidic linkages. They are major components of mucus, covering the luminal surfaces of epithelial respiratory, gastrointestinal and reproductive tracts, and respons...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.200300099
更新日期:2004-05-17 00:00:00
abstract::The preclinical development of nanomedicines raises several challenges and requires a comprehensive characterization. Among them is the evaluation of the biodistribution following systemic administration. In previous work, the biocompatibility and in vitro targeting ability of a glycol chitosan (GC) based nanogel have...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500267
更新日期:2016-03-01 00:00:00
abstract::A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins w...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100351
更新日期:2012-07-01 00:00:00
abstract::Cell adhesion to a scaffold is a prerequisite for tissue engineering. Many studies have been focused on enhancing cell adhesion to synthetic materials that are used for scaffold fabrication. In this study, we applied an avidin-biotin binding system to enhance chondrocyte adhesion to biodegradable polymers. Biotin mole...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400144
更新日期:2005-03-15 00:00:00
abstract::Two different composite scaffolds, solid-freeform-fabricated PCL/β-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular ac...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200393
更新日期:2013-05-01 00:00:00
abstract::In situ self-assemblies of new biodegradable triblock PLLA-b-PBS-b-PLLA and PDLA-b-PBS-b-PDLA have been investigated in acetonitrile solution. At first, two series of PLLA-b-PBS-b-PLLA and PDLA-b-PBS-b-PDLA, respectively denoted as the P and Q triblock copolyester series, were prepared with fixed PBS block ((overline)...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400227
更新日期:2005-06-24 00:00:00
abstract::Poly(N-methylvinylamines) with secondary amines can form complexes with plasmid DNA (pDNA) and provide transfection efficiency in HeLa cells in the same order as linear polyethyleneimine but with higher cell viability. Chemical modifications of poly(N-methylvinylamine) backbones are performed to further improve transf...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700353
更新日期:2018-04-01 00:00:00
abstract::Desferrioxamine (DFO) is a clinically approved, high affinity iron chelator used for the treatment of iron overload. Due to its short half-life and toxicity, DFO is administered for 8-12 h per day, 5-7 d per week. In this manuscript, the influence of molecular properties of hyperbranched polyglycerol (HPG)-DFO conjuga...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600244
更新日期:2017-03-01 00:00:00
abstract::Currently, there is no gadolinium-based contrast agent available for conventional magnetic resonance imaging (MRI) detection of amyloidal beta (Aβ) plaques in Alzheimer's disease (AD). Its timely finding would be vital for patient survival and quality of life. Curcumin (CUR), a common Indian spice effectively binds to...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500062
更新日期:2015-09-01 00:00:00
abstract::Three types of multilayer films made from poly(L-lysine)/hyaluronan, chitosan/hyaluronan, and poly(allylamine hydrochloride)/poly(L-glutamic acid), were used to investigate the interplay between film mechano-chemical properties and cell adhesion. We showed that C2C12 myoblast adhesion and proliferation depended on the...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000301
更新日期:2011-01-10 00:00:00
abstract::To explore the potential of a star-shaped 8-armed poly(ethylene glycol)35K-block-poly(L-lactide)37K (8-armed PEG35K-b-PLLA37K: M(n) of PEG = 35 000, M(n) of PLLA = 37 000) film as a novel bioabsorbable adhesion-prevention membrane, the water structure, surface contact angle, protein adsorption, and cell and platelet a...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500249
更新日期:2006-06-16 00:00:00
abstract::Although chronic lymphocytic leukemia (CLL) is the most common adult leukemia in Western world, it remains incurable with conventional chemotherapeutic agents. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is an antitumor candidate in cancer therapy. This study examines the proapoptotic effects...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600169
更新日期:2017-05-01 00:00:00
abstract::This work focuses on the development of ultrasound contrast vesicles for ultrasound-mediated enhanced transfection of nucleic acids in the cancer cells and projects its application as a tool for diagnostic imaging. The ultrasound contrast vesicles are stable, anionic, nanoscaled vesicles with ultrasound contrast equiv...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000173
更新日期:2020-12-01 00:00:00
abstract::The aim of this study is to design a novel two-component hybrid scaffold using the fibrin/alginate porous hydrogel Smart Matrix combined to a backing layer of plasma polymerized polydimethylsiloxane (Sil) membrane to make the fibrin-based dermal scaffold more robust for the treatment of the clinically challenging pres...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700185
更新日期:2017-11-01 00:00:00
abstract::Thermosensitive macroporous scaffolds of poly(N-isopropylacrylamide) (polyNIPA) loaded with chitosan/bemiparin nanoparticles are prepared by the free radical polymerization in cryogenic conditions. Chitosan/bemiparin nanoparticles of 102 ± 6.5 nm diameter are prepared by complex coacervation and loaded into polyNIPA c...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300184
更新日期:2013-11-01 00:00:00
abstract::Fluorescent dye labeled unfunctionalized and functionalized poly(n-butylcyanoacrylate) nanoparticles were prepared using a miniemulsion technique. Amino acid and methoxyPEG functionalization could be introduced by using aqueous solutions as an initiator for the anionic polymerization in the heterophase. All the partic...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700046
更新日期:2007-07-09 00:00:00
abstract::Redox-responsive micelles are versatile nanoplatforms for on-demand drug delivery, but the in situ evaluation of drug release is challenging. Fluorescence resonance energy transfer (FRET) technique shows potential for addressing this, while the aggregation-caused quenching effect limits the assay sensitivity. The aim ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700339
更新日期:2018-03-01 00:00:00
abstract::Amino acid ester substituted polyphosphazenes are osteoactive benefiting from their phosphorus-containing chemical structure, which highlights interests in bone tissue engineering. To correlate their chemical structures with cell activities, in this study, poly[(ethyl alanato)0.3 (ethyl glycinato)0.7 phosphazene] (PAG...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800464
更新日期:2019-06-01 00:00:00
abstract::Melanin is an effective absorber of light and can extend to near infrared (NIR) regions. In this study, a natural melanin is presented as a photothermal therapeutic agent (PTA) because it provides a good photothermal conversion efficiency, shows biodegradability, and does not induce long-term toxicity during retention...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600371
更新日期:2017-05-01 00:00:00
abstract::Diblock and multiblock copolymers composed of a poly(D,L-lactide) (PLA) or poly(trimethylene carbonate) (PTMC) core with a hydrophilic chain of poly(ethylene glycol) (PEG) were prepared. These copolymers, in which the core is connected to PEG through a polyfunctional molecule such as citric, mucic, or tartaric acid, m...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600165
更新日期:2006-12-08 00:00:00
abstract::Novel block copolymers using the monomers 5-(2-methoxyethyoxymethyl)-5-methyl-[1,3]-dioxa-2-one (TMCM-MOE1OM) as a hydrophilic segment and lactides as a hydrophobic segment were designed in order to prepare controllable degradation polymers by dynamic polymer rearrangement based on the hydrophilicity. When the copolym...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200143
更新日期:2012-10-01 00:00:00
abstract::The repair of large crushed or sectioned segments of peripheral nerves remains a challenge in regenerative medicine due to the complexity of the biological environment and the lack of proper biomaterials and architecture to foster reconstruction. Traditionally such reconstruction is only achieved by using fresh human ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201500367
更新日期:2016-04-01 00:00:00
abstract::Current methods of tissue fixation rely on mechanical-related technologies developed from the clothing and carpentry industries. Herein, a novel bioadhesive method that allows tuneable adhesion and is also applicable to biodegradable polyester substrates is described. Diazirine is the key functional group that allows ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300380
更新日期:2014-04-01 00:00:00
abstract::Open-pore biodegradable foams with controlled porous architectures were prepared by combining gas foaming and microparticulate templating. Microparticulate composites of poly(epsilon-caprolactone) (PCL) and micrometric sodium chloride particles (NaCl), in concentrations ranging from 70/30 to 20/80 wt.-% of PCL/NaCl we...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700278
更新日期:2008-07-07 00:00:00
abstract::The biorecognition-based control of attachment/detachment of MCF-7 cancer cells from polymer-coated surfaces is demonstrated. A glass surface is coated with a thermoresponsive statistical copolymer of poly(N-isopropylacrylamide-co-acrylamide) [p(NIPAm-co-Am)], which is end-capped with the Gly-Arg-Gly-Asp-Ser (GRGDS) p...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000277
更新日期:2020-11-04 00:00:00