Abstract:
:A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins when the alginate drop separates from the mineral oil shell and comes into contact with Ca(2+) ions in the continuous phase. Alginate hydrogel microparticles with diameters ranging from 60 to 230 µm are obtained. 65% of the cells encapsulated in the alginate microparticles were viable after one week. The technique provides a useful means to encapsulate the living cells in monodisperse hydrogel microparticles.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Martinez CJ,Kim JW,Ye C,Ortiz I,Rowat AC,Marquez M,Weitz Ddoi
10.1002/mabi.201100351subject
Has Abstractpub_date
2012-07-01 00:00:00pages
946-51issue
7eissn
1616-5187issn
1616-5195journal_volume
12pub_type
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