Abstract:
:Cells in physiological 3D environments differ considerably in morphology and differentiation from those in 2D tissue culture. Naturally derived polymer systems are frequently used to study cells in 3D. These 3D matrices are complex with respect to their chemical composition, mechanical properties, and geometry. Therefore, there is a demand for well-defined 3D scaffolds to systematically investigate cell behavior in 3D. Here, fabrication techniques, materials, architectures, biochemical functionalizations, and mechanical properties of 3D scaffolds are discussed. In particular, work focusing on single cells and small cell assemblies grown in tailored synthetic 3D scaffolds fabricated by computer-based techniques are reviewed and the influence of these environments on cell behavior is evaluated.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Greiner AM,Richter B,Bastmeyer Mdoi
10.1002/mabi.201200132subject
Has Abstractpub_date
2012-10-01 00:00:00pages
1301-14issue
10eissn
1616-5187issn
1616-5195journal_volume
12pub_type
杂志文章,评审abstract::The nanostructure of a biomaterial surface has strong influence on cell behavior. The migration of cells on nanostructured surfaces, however, has not been investigated so far. In this study, we used PCL/PEG diblock copolymers as model surfaces to examine the effect of nanoislands on migration of different cells, inclu...
journal_title:Macromolecular bioscience
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doi:10.1002/mabi.200600248
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abstract::A nanoformulation composed of curdlan, a linear polysaccharide of 1,3-β-linked d-glucose units, hydrogen bonded to poly(γ -glutamic acid) (PGA), was developed to stimulate macrophage. Curdlan/PGA nanoparticles (C-NP) are formulated by physically blending curdlan (0.2 mg mL-1 in 0.4 m NaOH) with PGA (0.8 mg mL-1 ). For...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000358
更新日期:2021-01-01 00:00:00
abstract::Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200471
更新日期:2013-05-01 00:00:00
abstract::A simple, robust one pot procedure for the preparation of waterborne multifunctional poly(vinyl amine)s (PVAms) is presented. By post-polymerization modification of PVAm with a bifunctional coupler and functional couplers cationic, reactive azetidinium groups, and alkyl chains are introduced in the side chains of PVAm...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300576
更新日期:2014-08-01 00:00:00
abstract::3D printing has become an essential part of bone tissue engineering and attracts great attention for the fabrication of bioactive scaffolds. Combining this rapid manufacturing technique with chemical precipitation, biodegradable 3D scaffold composed of polymer matrix (polylactic acid and polyethylene glycol), ceramics...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800068
更新日期:2018-06-01 00:00:00
abstract::Unique features of nanofibers provide enormous potential in the field of biomedical and healthcare applications. Many studies have proven the extreme potential of nanofibers in front of current challenges in the medical and healthcare field. This review highlights the nanofiber technologies, unique properties, fabrica...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201800256
更新日期:2019-02-01 00:00:00
abstract::Transparent, biodegradable, mechanically robust, and surface-patterned silk films were evaluated for the effect of surface morphology on human corneal fibroblast (hCF) cell proliferation, orientation, and ECM deposition and alignment. A series of dimensionally different surface groove patterns were prepared from optic...
journal_title:Macromolecular bioscience
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doi:10.1002/mabi.200900452
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abstract::pH-responsive amphiphilic graft macromolecules consisting of a polyphosphazene backbone, hydrophilic PEG branches and pH-sensitive DPA were successfully synthesized and characterized. The copolymer can self-assemble into vesicles in an aqueous solution with unique inner structure and homogeneously encapsulate both lip...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000333
更新日期:2011-03-10 00:00:00
abstract::In this paper we report on the synthesis of diversified linear polyamine architectures with different chain lengths and compositions and their interaction with phosphate groups of DNA/siRNA. The polyplex formation between model nucleotide (dsDNA) and these linear polyamines has been determined at different nitrogen to...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000082
更新日期:2010-09-09 00:00:00
abstract::[Image: see text] Oligosaccharides at cell surfaces are known to play a critical role in many biological processes such as biorecognition, interactions between cells and with artificial surfaces, immune response, infection and inflammation. In order to facilitate studies of the role of sugars, an increasing number of ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600090
更新日期:2006-08-07 00:00:00
abstract::The effects of RGD peptide conjugation to alginate hydrogel on the adipogenic differentiation of ASCs was investigated. After 3 d of culture, RGD-modified alginate hydrogels significantly stimulated FAK and integrin α1 gene expressions and vinculin expression in ASCs. In addition, RGD-modified alginate hydrogels signi...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000479
更新日期:2011-05-12 00:00:00
abstract::Photo-crosslinking and self-healing have received considerable attention for the design of intelligent materials. A novel photostimulated, self-healing, and cytocompatible hydrogel system is reported. A coumarin methacrylate crosslinker is synthesized to modify the polyacrylamide-based hydrogels. With the [2+2] cyclo-...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500457
更新日期:2016-09-01 00:00:00
abstract::L-, P-, and E-Selectin are cell adhesion molecules that play a crucial role in leukocyte recruitment from the blood stream to the afflicted tissue in an acute and chronic inflammatory setting. Since selectins mediate the initial contact of leukocytes to the vascular endothelium, they have evolved as a valuable therape...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100051
更新日期:2011-08-11 00:00:00
abstract::Primary human megakaryocytes, the precursor cells of platelets, are difficult to collect and cultivate. Polymers that enrich these cells without affecting their regulation or function are of interest for basic research as well as for cord blood transplantation purposes since co-transplantation of enriched megakaryocyt...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200368
更新日期:2013-04-01 00:00:00
abstract::In search for effective non-viral gene vectors for the delivery of siRNA, a copolymer was designed and synthesized by grafting hydrophobic poly(gamma-benzyl L-glutamate) segment (PBLG) to hyperbranched polyethylenimine (PEI-PBLG). PEI-PBLG could efficiently deliver siRNA to cells to silence the targeted gene. Markedly...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900249
更新日期:2009-12-08 00:00:00
abstract::A microfluidic technique is described to encapsulate living cells in alginate hydrogel microparticles generated from monodisperse double-emulsion templates. A microcapillary device is used to fabricate double emulsion templates composed of an alginate drop surrounded by a mineral oil shell. Hydrogel formation begins w...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100351
更新日期:2012-07-01 00:00:00
abstract::Multivalent carbohydrate-lectin interactions play a crucial role in bacterial infection. Biomimicry of multivalent glycosystems represents a major strategy in the repression of bacterial growth. In this study, a new kind of glycopeptide (Naphthyl-Phe-Phe-Ser-Tyr, NMY) scaffold with mannose modification is designed and...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201900124
更新日期:2019-09-01 00:00:00
abstract::Research and development in the design, synthesis, modification, evaluation, and characterization of polysaccharide-based bioactive polymeric materials for guiding and promoting new tissue in-growth is reviewed. Emphasis is given in this interdisciplinary field of tissue engineering (TE) with particular reference to b...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201200409
更新日期:2013-04-01 00:00:00
abstract::Novel water-insoluble, and reduction-responsive nonwoven scaffolds were fabricated from gamma-PGA and tested in cell culture. An electrospinning method was developed to produce scaffolds of fibers with diameters of 0.05-0.5 microm. Crosslinking of the fibers with cystamine in the presence of EDC resulted in water-inso...
journal_title:Macromolecular bioscience
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abstract::A nanoscale biodegradable polymersome with pH-tuning on-off membrane is prepared via the self-assembly of poly(β-amino ester)-based amphiphilic copolymers. The pH-sensitive polymersome-like vesicle structure includes two layers that can encapsulate either hydrophobic or hydrophilic therapeutic drugs at physiological p...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200468
更新日期:2013-07-01 00:00:00
abstract::Chronic non-healing wounds show delayed and incomplete healing processes and in turn expose patients to a high risk of infection. Treatment currently focuses on dressings that prevent microbial infiltration and keep a balanced moisture and gas exchange environment. Antibacterial delivery from dressings has existed for...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201300561
更新日期:2014-06-01 00:00:00
abstract::The role viscoelasticity in fibrotic disease progression is an emerging area of interest. Here, a fast-relaxing hydrogel system is exploited to investigate potential crosstalk between calcium signaling and mechanotransduction. Poly(ethylene glycol) (PEG) hydrogels containing boronate and triazole crosslinkers are synt...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000268
更新日期:2020-12-01 00:00:00
abstract::Bio-based fibrous nanocomposites of cellulose nanofibres and non-crosslinked/crosslinked collagen were prepared by in situ pH-induced fibrillation of collagen phase and sterilized using gamma rays at 25 KGy. Collagen phase is crosslinked using genipin, a bio-based crosslinker that introduces flexible crosslinks. Micro...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200317
更新日期:2013-03-01 00:00:00
abstract::When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue" that binds strongly and q...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200092
更新日期:2012-08-01 00:00:00
abstract::The present study describes the synthesis of novel polypeptides containing O-phospho-L-threonine [Thr(PO(3)H(2))] and L-aspartic acid. Random copolypeptides copoly[Thr(PO(3)H(2))(X)Asp(Y)] (X:Y = 25:75, 50:50, 75:25), were conventionally prepared by copolymerization of Thr(PO(3)Ph(2)) N-carboxyanhydride (NCA) and Asp(...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500211
更新日期:2006-03-14 00:00:00
abstract::New data are constantly gathered to show the role of oxidative stress and the involvement of reactive oxygen species in the pathogenesis of degenerative diseases. InsP6 is able to coordinate iron metal in order to prevent iron-catalyzed free radical formation. The aim of the present paper is to describe a new syntheti...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200500136
更新日期:2005-11-04 00:00:00
abstract::The preclinical development of nanomedicines raises several challenges and requires a comprehensive characterization. Among them is the evaluation of the biodistribution following systemic administration. In previous work, the biocompatibility and in vitro targeting ability of a glycol chitosan (GC) based nanogel have...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500267
更新日期:2016-03-01 00:00:00
abstract::Two chondrogenic factors, Dex and TGF-beta1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-beta1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were s...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200800187
更新日期:2009-03-10 00:00:00
abstract::Three different microbial wild-type strains are compared with respect to their potential as industrial scale polyhydroxyalkanoate (PHA) producers from the feed stock whey lactose. The halophilic archaeon Haloferax mediterranei as well as two eubacterial strains (Pseudomonas hydrogenovora and Hydrogenophaga pseudoflava...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600211
更新日期:2007-02-12 00:00:00
abstract::Artificial ECMs that not only closely mimic the hybrid nature of the natural ECM but also provide tunable material properties and enhanced biological functions are attractive candidates for tissue engineering applications. This review summarizes recent advances in developing multicomponent hybrid hydrogels by integrat...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.200800284
更新日期:2009-02-11 00:00:00