Abstract:
:Poly(vinyl alcohol)-pyrene-anti-metadherin (PVA-Py-(Anti-MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA-Py prepared by "Click chemistry" approach is targeted by Anti-MTDH antibody and applied to the MCF-7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non-targeted structure (PVA-Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Medine EI,Odaci D,Gacal BN,Gacal B,Sakarya S,Unak P,Timur S,Yagci Ydoi
10.1002/mabi.201000009subject
Has Abstractpub_date
2010-06-11 00:00:00pages
657-63issue
6eissn
1616-5187issn
1616-5195journal_volume
10pub_type
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