Abstract:
:Primary human megakaryocytes, the precursor cells of platelets, are difficult to collect and cultivate. Polymers that enrich these cells without affecting their regulation or function are of interest for basic research as well as for cord blood transplantation purposes since co-transplantation of enriched megakaryocyte concentrates increase the success of stem cell therapy. Herein, polymer microarrays were used for the discovery of substrates for MEG-01 cells, with five polymers identified which selectively bound cells of the megakaryocytic lineage. Flow cytometry and miRNA profiling revealed that immobilisation had only a minor effect on the cellular maturation status, making the identified substrates potential candidates for concentrating megakaryocytes from patients prior to transplantation.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Hansen A,Corless S,Cleland A,Petrik J,Gilbert N,Bradley Mdoi
10.1002/mabi.201200368subject
Has Abstractpub_date
2013-04-01 00:00:00pages
437-43issue
4eissn
1616-5187issn
1616-5195journal_volume
13pub_type
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