Abstract:
:The present study investigates the effect of galactosylated gelatin on encapsulated HepG2 cells. Methacrylamide modified gelatin is evaluated and compared with its galactosylated counterpart with respect to effects on viability, morphological characteristics, proliferation, and the expression of hepatocyte specific markers. The research reveals that further modifications of methacrylamide modified gelatin are possible without affecting the survival of the encapsulated cells (viability of 90%). Moreover, the study demonstrates a clear and long-term (up to 21 d) improvement in hepatocyte specific gene expression when the cells are encapsulated in the galactosylated gelatin. It is concluded that the use of galactosylated gelatin derivates supports the hepatocyte phenotype.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Gevaert E,Billiet T,Declercq H,Dubruel P,Cornelissen Rdoi
10.1002/mabi.201300320subject
Has Abstractpub_date
2014-03-01 00:00:00pages
419-27issue
3eissn
1616-5187issn
1616-5195journal_volume
14pub_type
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