Abstract:
:Plasmid DNA (pDNA) is encapsulated into biocompatible microgels by an inverse microemulsion polymerization method. Plasmid DNA and doxorubicin are successfully released from pDNA microgels and their release profiles are characterized by appropriate release models. The co-delivery of genes and drugs from the microgels is evaluated as an enhancer of clinical treatment. Moreover, the release of the encapsulated pDNA is capable of transfection in vitro resulting in the expression of p53 protein. As a whole, a novel pDNA-based system is described that may find biomedical uses, especially in the cancer treatment through the combined action of chemotherapy and gene delivery approach.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Costa D,Valente AJ,Miguel MG,Queiroz Jdoi
10.1002/mabi.201200096subject
Has Abstractpub_date
2012-09-01 00:00:00pages
1243-52issue
9eissn
1616-5187issn
1616-5195journal_volume
12pub_type
杂志文章abstract::This work focuses on the development of ultrasound contrast vesicles for ultrasound-mediated enhanced transfection of nucleic acids in the cancer cells and projects its application as a tool for diagnostic imaging. The ultrasound contrast vesicles are stable, anionic, nanoscaled vesicles with ultrasound contrast equiv...
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