Abstract:
:The use of glycopolymer-functionalized resins (Resin-Glc), as a solid support, in column mode for bacterial/protein capture and quantification is explored. The Resin-Glc is synthesized from commercially available chloromethylated polystyrene resin and glycopolymer, and is characterized by fourier transform infrared spectroscopy, thermogravimetry, and elemental analysis. The percentage of glycopolymer functionalized on Resin-Glc is accounted to be 5 wt%. The ability of Resin-Glc to selectively capture lectin, Concanavalin A, over Peanut Agglutinin, reversibly, is demonstrated for six cycles of experiments. The bacterial sequestration study using SYBR (Synergy Brands, Inc.) Green I tagged Escherichia coli/Staphylococcus aureus reveals the ability of Resin-Glc to selectively capture E. coli over S. aureus. The quantification of captured cells in the column is carried out by enzymatic colorimetric assay using methylumbelliferyl glucuronide as the substrate. The E. coli capture studies reveal a consistent capture efficiency of 105 CFU (Colony Forming Units) g-1 over six cycles. Studies with spiked tap water samples show satisfactory results for E. coli cell densities ranging from 102 to 107 CFU mL-1 . The method portrayed can serve as a basis for the development of a reusable solid support in capture and detection of proteins and bacteria.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Ajish JK,Abraham HM,Subramanian M,Kumar KSAdoi
10.1002/mabi.202000342subject
Has Abstractpub_date
2020-12-18 00:00:00pages
e2000342eissn
1616-5187issn
1616-5195pub_type
杂志文章abstract::Although recently a growing number of reports demonstrate that topography or geometry of the substrate also plays an important role in the fate of the stem cells, most of these studies are usually completed by a few distinct patterns such as simple lines, posts, etc. As a result, there is a lack of quantitative analys...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000477
更新日期:2011-07-07 00:00:00
abstract::The polyethylenimine (PEI) derivatives (PTn) are prepared by treating PEI25k with Tris(hydroxymethyl) acrylamidomethane via the Michael addition. These PTns can effectively condense nucleic acids into nanosized particles with positive surface charges. The PTns show lower cytotoxicity and better serum-resistant capacit...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200346
更新日期:2013-04-01 00:00:00
abstract::Surface structural characterisation of three different starch-based blends (with poly[ethylene-co-(vinyl alcohol)], cellulose acetate and polycaprolactone) was carried out. The results show that there is a difference between the bulk and the surface composition of all studied blends. Two different hypotheses were inve...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700076
更新日期:2008-02-11 00:00:00
abstract::A facile and easily reproducible technique for assembling biohybrid nanoparticles is a core feature that is highly desired for biomedical applications, considering the nature and limited lifespan of the biopolymers used. Here we show a simple and effective method to enfold single-walled carbon nanotubes (SWNTs) using ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200900349
更新日期:2010-03-10 00:00:00
abstract::Peptide dendrimers assembled by solid-phase peptide synthesis using a branching diamino acid at every 2(nd) or 3(rd) position provide readily accessible synthetic model systems for proteins and enzymes. They adopt a globular shape by topology rather than by folding. Peptide dendrimers of 2(nd) and 3(rd) generation fun...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200700036
更新日期:2007-08-07 00:00:00
abstract::A combination of hard sphere and high internal phase emulsion templating gives a platform for synthesizing hierarchically porous polymers with a unique topology exhibiting interconnected spherical features on multiple levels. Polymeric spheres are fused by thermal sintering to create a 3D monolithic structure while an...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700306
更新日期:2018-02-01 00:00:00
abstract::A poly(o-methoxyaniline) (POMA)/DNA [weight fraction of DNA (W(DNA)) = 0.45] hybrid was prepared by mixing their solutions in sterilized double distilled water. The solution turned green upon aging for a longer time, and the doping of POMA by DNA was complete after about 15 d of aging. The doping was confirmed from th...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400185
更新日期:2005-05-23 00:00:00
abstract::Bleeding complications usually cause significant morbidity and mortality in civilian and military populations. In clinical application, hemostatic sponges, gauzes, hydrogel, and bandages are widely used as the traditional effective hemostatic products for hemorrhage. However, the traditional hemostatic devices or agen...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201900370
更新日期:2020-04-01 00:00:00
abstract::Mucoadhesive NP drug carriers have attracted substantial interest as a potential treatment for anterior eye diseases. NPs composed of PLA-Dex surface functionalized with a mucoadhesive ligand, PBA, were developed as drug carriers with particle sizes ranging from 25 to 28 nm. Using CycA as a model drug, we showed that ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200216
更新日期:2012-12-01 00:00:00
abstract::The fabrication of 3D cell microenvironments exploiting versatile, long-term stable passivating poly(acryl amide) brushes in a microwell format and the study of the behavior of fibroblast and pancreatic tumor cells in wells of systematically varied shape and size is reported. The microwells, which are obtained by comb...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600451
更新日期:2017-05-01 00:00:00
abstract::Aggregation of denatured or unfolded proteins establishes a large energy barrier to spontaneous recovery of protein form and function following traumatic injury, tissue cryopreservation, and biopharmaceutical storage. Some tissues utilize small heat shock proteins (sHSPs) to prevent irreversible aggregation, which all...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600217
更新日期:2017-02-01 00:00:00
abstract::Novel block copolymers using the monomers 5-(2-methoxyethyoxymethyl)-5-methyl-[1,3]-dioxa-2-one (TMCM-MOE1OM) as a hydrophilic segment and lactides as a hydrophobic segment were designed in order to prepare controllable degradation polymers by dynamic polymer rearrangement based on the hydrophilicity. When the copolym...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200143
更新日期:2012-10-01 00:00:00
abstract::O-carboxymethylchitin (molecular weight = 1.07 x 10(5), degree of carboxymethylation = 80%, degree of N-acetylation = 91%) was chemically attached to superoxide dismutase by the formation of amide linkages through a carbodiimide catalyzed reaction. The glycosidated enzyme contained about 1.8 mole of polysaccharide per...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200400114
更新日期:2005-02-23 00:00:00
abstract::The preparation of mono- and multifilament sutures incorporating ibuprofen as an anti-inflammatory agent is considered. Poly(p-dioxanone) monofilament samples can be loaded by a molecular diffusion process using a swelling agent such as dichloromethane. The mechanical properties have been measured and have not shown a...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600084
更新日期:2006-09-15 00:00:00
abstract::Cell-free approaches to in situ tissue engineering require materials that are mechanically stable and are able to control cell-adhesive behavior upon implantation. Here, the development of mechanically stable grafts with non-cell adhesive properties via a mix-and-match approach using ureido-pyrimidinone (UPy)-modified...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500278
更新日期:2016-03-01 00:00:00
abstract::This work focuses on the development of ultrasound contrast vesicles for ultrasound-mediated enhanced transfection of nucleic acids in the cancer cells and projects its application as a tool for diagnostic imaging. The ultrasound contrast vesicles are stable, anionic, nanoscaled vesicles with ultrasound contrast equiv...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000173
更新日期:2020-12-01 00:00:00
abstract::Herein, a new series of non-ionic dendritic and carbohydrate based amphiphiles is synthesized employing biocompatible starting materials and studied for supramolecular aggregate formation in aqueous solution. The dendritic amphiphiles 12 and 13 possessing poly(glycerol) [G2.0] as hydrophilic unit and C-10 and C-18 hyd...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800019
更新日期:2018-07-01 00:00:00
abstract::Redox-responsive micelles are versatile nanoplatforms for on-demand drug delivery, but the in situ evaluation of drug release is challenging. Fluorescence resonance energy transfer (FRET) technique shows potential for addressing this, while the aggregation-caused quenching effect limits the assay sensitivity. The aim ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201700339
更新日期:2018-03-01 00:00:00
abstract::Multicomponent systems for self-assembled molecular gels provide huge opportunities to generate collective or new functions that are not inherent in individual single-component gels. However, gelation tends to require careful and complicated procedures, because, among a myriad of kinetically trapped structures related...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.202000187
更新日期:2020-09-01 00:00:00
abstract::Chitosan is non-toxic, biocompatible, and biodegradable polysaccharide from renewable resources, known to have inherent antibacterial activity, which is mainly due to its polycationic nature. The combining of all assets of chitosan and its derivatives with the unique properties of electrospun nanofibrous materials is ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章,评审
doi:10.1002/mabi.201300058
更新日期:2013-07-01 00:00:00
abstract::Oligomerization of thiol-unprotected L-cysteine ethyl ester (Cys-OEt) catalyzed by proteinase K in aqueous solution has been used to synthesize oligo(L-cysteine) (OligoCys) with a well-defined chemical structure and relatively large degree of polymerization (DP) up to 16-17 (average 8.8). By using a high concentration...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201500255
更新日期:2016-01-01 00:00:00
abstract::Novel chitosan (CHS) and cellulose sulfates (CSs) are studied regarding their mitogenic activity and their protective effect against proteolytic digestion of FGF-2. An intermediate degree of sulfation (DS(S) ) and lower concentration of CHS have superior effect on 3T3 cell growth while the mitogenic activity of CS inc...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201100518
更新日期:2012-06-01 00:00:00
abstract::Microparticle drug carriers made of biodegradable functional polyesters were produced. The polyesters consist of a poly(ε-caprolactone) backbone bearing pendant acryloyloxy and methacryloyloxy groups. Stable microparticles were prepared via an oil/water emulsion-solvent evaporation technique eventually combined with a...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000023
更新日期:2010-08-11 00:00:00
abstract::Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201200471
更新日期:2013-05-01 00:00:00
abstract::Biopolymers are an attractive class of compounds for being used in biomedical applications as they are widely available from biomass. Their drawback is the lack of mechanical stability and the ability to tune this properly. Covalent chemical cross-linking is an often used approach but it limits usability due to legisl...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201600214
更新日期:2016-11-01 00:00:00
abstract::Solution-grown chain-folded lamellar single crystals of poly(tetramethylene adipate) (PTMA) were prepared from a dilute solution of 2-methyl-1-propanol by isothermal crystallization. PTMA crystals were hexagonal-shaped and polyethylene decoration of the crystals resulted in a "six cross-sector" surface morphology and ...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200300086
更新日期:2004-03-15 00:00:00
abstract::The effects of RGD peptide conjugation to alginate hydrogel on the adipogenic differentiation of ASCs was investigated. After 3 d of culture, RGD-modified alginate hydrogels significantly stimulated FAK and integrin α1 gene expressions and vinculin expression in ASCs. In addition, RGD-modified alginate hydrogels signi...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201000479
更新日期:2011-05-12 00:00:00
abstract::Branched polyethylenimine (25 kDa) is thiolated and compared with redox-sensitive crosslinked derivatives. Both polymers thiol contents are assessed; the thiolated polymers have 390-2300 mmol SH groups/mol, whereas the crosslinked polymers have lower thiol contents. Cytotoxicity assays show that both modified polymers...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201300103
更新日期:2013-09-01 00:00:00
abstract::The synthesis of a zwitterionic polymer, poly-sulfobetaine (poly-SPB), which shows remarkable efficiency in the suppression of insulin aggregation is described. Hydrophobic modification of the polymer results in almost complete inhibition at very low polymer concentrations. Further studies reveal that these polymers f...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.201800016
更新日期:2018-06-01 00:00:00
abstract::Three different microbial wild-type strains are compared with respect to their potential as industrial scale polyhydroxyalkanoate (PHA) producers from the feed stock whey lactose. The halophilic archaeon Haloferax mediterranei as well as two eubacterial strains (Pseudomonas hydrogenovora and Hydrogenophaga pseudoflava...
journal_title:Macromolecular bioscience
pub_type: 杂志文章
doi:10.1002/mabi.200600211
更新日期:2007-02-12 00:00:00