Abstract:
:Branched polyethylenimine (25 kDa) is thiolated and compared with redox-sensitive crosslinked derivatives. Both polymers thiol contents are assessed; the thiolated polymers have 390-2300 mmol SH groups/mol, whereas the crosslinked polymers have lower thiol contents. Cytotoxicity assays show that both modified polymers give lower hemolysis than unmodified PEI. Increased thiol content increases gene transfer efficiency but also elevates cytotoxicity. Crosslinking improves plasmid DNA condensation and enhances transfection efficiency, but extensive crosslinking overstabilizes the polyplexes and decreases transfection, emphasizing the need to balance polyplex stabilization and unpacking. Thus, at low levels of crosslinking, 25 kDa PEI can be an efficient redox-sensitive carrier system.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Aravindan L,Bicknell KA,Brooks G,Khutoryanskiy VV,Williams ACdoi
10.1002/mabi.201300103subject
Has Abstractpub_date
2013-09-01 00:00:00pages
1163-73issue
9eissn
1616-5187issn
1616-5195journal_volume
13pub_type
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